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A Study on the Growth and Disease of Chondrus ocellatus in Korea (한국산 진두발, Chondrus ocellatus의 생장과 질병에 대한 연구)

  • Lee, Soon Jeong;Park, Myoung-Ae;Ogandaga-Maranguy, Cyr Abel;Park, Seo Kyoung;Kim, Hoikyung;Kim, Young Sik;Choi, Han Gil
    • Journal of fish pathology
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    • v.26 no.3
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    • pp.265-274
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    • 2013
  • The growth, reproduction, gametophyte(G)/tetrasporophyte(T) ratio, and diseases of Chondrus ocellatus populations were examined at 3 sites (Samcheok, Youngduk, Pohang) of East coast and at 2 sites (Hakampo, Manripo) of West coast in between July and August, 2013. Average plant lengths were 6.10~9.69 cm and it was minimum at Manripo and maximum at Pohang population. In general, average plant length and weight of C. ocellatus were greater on East coast than West coast populations. The proportion of vegetative plant was between 26.7~66.7 %, and G/T ratio of total plants including vegetative plants after testing resorcinol method was 3:2 on the East coast where is gametophyte dominant area. However, G/T ratio was 1:1 at Hakampo and 1:2.3 at Manripo representing tetrasporophyte dominance. In the present study, Korean C. ocellatus have various diseases (white colour and green colour), an endophytic alga(Ulvella sp.), and many epiphytic macroalgae and diatoms. Healthy C. ocellatus plants were about 20~40 % in summer population and most of plants had disease. Chondrus ocellatus had a filamentous green alga, endophytic Ulvella sp. which was not identified. The endophyte is easily observed in C. crispus growing in Europe and Canada and it is recognised as a pathogen destroying population and reducing yield of C. crispus. Thus more interest and research on the endophytic algae and disease of C. ocellatus are required.

Seasonal Variations of Eelgrass (Zostera marina) and Epiphytic Algae in Eelgrass Beds in Kwangyang Bay (광양만 잘피밭에서 잘피와 착생해조류의 계절 변동)

  • HUK Sung-Hoi;KwAk Seok Nam;NAM Ki Wan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.1
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    • pp.56-62
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    • 1998
  • Seasonal variations of eelgrass and epiphytic algae in eelgrass beds in Kwangyang Bay were studied from January 1994 to December 1994. A peak of the shoot length and standing crop of eelgrass occurred in summer, but low values were observed in fall and winter. The dominant species of epiphytic algae were Callophyllis rhynchocarpa and Champia sp. In spring and summer, while Polysiphonia japonica and Lomentaria hakodatensis in fall and winter. In contrast to the eelgrass, the standing crop of epiphytic algae showed a minimum in summer. There was a gradual increase in the standing crop of epiphytic algae during fall, and a peak of standing crop occurred in winter. Epiphytic algae accounted for approximately $15\~20\%$ of total plant standing crops of the eelgrass meadows. Correlation analysis with environmental factors indicated that temperature influences on both the standing crop of eelgrass and epiphytic algae. There was a positive relationship between the standing crop of eelgrass and temperature, while there was a reverse relationship between that of epiphytic algae and temperature.

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An Increment of Crossing Efficiency with Consideration of Pollen Viability Analysis in Rose (장미 교배 효율 증대를 위한 화분 임성 검정)

  • Hwang, Yoon Jung;Song, Chang Min;Kwon, Min Kyung;Kim, Sung Tae;Kim, Won Hee;Han, Youn Yol;Han, Tae Ho;Lim, Ki Byung
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.193-200
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    • 2010
  • Current rose cultivars are all composed of heterozygous genome due to long history of out crossing including interspecific hybridization. It has been adapted by artificial selection and crossing by breeders that mainly based on the crossing with fertile pollen derived from inter- or intra-specific hybridization. Pollen viability and germination ability tests provide valuable information for the designing of parentage for more successful breeding efficacy. In this study, we tested the pollen viability and germination ability in seven rose cultivars to find any relationship among several factors including pollen size, ploidy levels, and crossing compatibility. The pollen viability showed wide ranges from 39% 'Pinocchio' as minimum to 82% 'Scarlet Mimi' as maximum, whereas pollen germination rate were from 1% 'Mini Rosa' to 41% 'Scarlet Mimi' as a highest. Pollen size ranged from 41.3 to $45.4{\mu}m$ in large sized pollen and 30.7 to $37.4{\mu}m$ in small sized pollen. The mean diameter of large sized pollen is approximately 10-40% bigger than that of small sized pollen. There are positive relationships among ploidy level, total chromosome length, and pollen size. Crossing list showed that seed setting ratio and seed germination were related to pollen viability, pollen germination, and ploidy level.

Characteristics of Soil Water Runoff and Canopy Cover Subfactor in Sloped Land with Different Soil Texture (경사지 밭토양에서 강우량과 토성에 따른 물 유출 양상 및 수관피복인자 구명)

  • Lee, Hyun-Haeng;Ha, Sang-Keon;Hur, Seung-Oh;Jung, Kang-Ho;Park, Chan-Won;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.2
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    • pp.131-135
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    • 2007
  • This study was performed as an effort to reduce soil loss by investigating the phase of water flow according to soil texture and rainfall pattern and by determining the canopy cover subfactor in the RUSLE (revised universal soil loss equation). Red pepper was planted at the 15% sloped lysimeter of $2m{\times}5m{\times}0.5m$ ($width{\times}length{\times}depth$) with three different textured soils (loam, clay loam and sandy loam) and the relationship between amount and intensity of rainfall; soil loss and the amount of runoff; and amount of rainfall and runoff at different soil texture were measured at the experiment station of the National Institute of Agricultural Science and Technology (NIAST) during May to October of 2005. The amount of runoff increased with increasing amount of rainfall, showing difference in the relative increase rate of runoff at different soil texture. The increase rate of runoff with unit increase of rainfall for the lysimeter with red pepper was 0.44, 0.41 and 0.13 for loam, clayey loam and sandy loam, respectively. The minimum amount of rainfall for runoff was 23.53 mm for sandy loam, 10.35 mm for loam and 5.46 mm for clayey loam, respectively. The canopy cover subfactors of red pepper were 0.425, 0.459, and 0.478 for sandy loam, loam and clayey loam, respectively.

'Choyoung', Triticale Cultivar for Forage of Early-Heading, Resistance to Lodging and High Seed Production (조숙 내도복 종실 다수성 조사료용 트리티케일 품종 '조영')

  • Han, Ouk-Kyu;Park, Hyung-Ho;Park, Tae-Il;Oh, Young-Jin;Ahn, Jong-Woong;Ku, Ja-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.2
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    • pp.68-74
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    • 2019
  • 'Choyoung', a winter forage triticale cultivar (X Triticosecale Wittmack), was developed at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2015. The cultivar 'Choyoung' has the leaves of medium width, long length and green color and a medium grain of brown color. The heading date of the cultivar 'Choyoung' was April 30 which was 2 days earlier than that of check cultivar 'Shinyoung'. Its tolerance or resistance to cold, wet injury, powdery mildew, and leaf rust was similar to those of the check cultivar. But the resistance to the lodging of cultivar 'Choyoung' was stronger than that of the check. The average forage fresh and dry matter yield of cultivar 'Choyoung' at milk-ripe stages were 40.5 and $15.7MT\;ha^{-1}$, respectively, which were similar to those (40.3 and $16.1MT\;ha^{-1}$) of the check cultivar. The silage quality of 'Choyoung' was higher than that of the check cultivar 'Shinyoung' in crude protein content (5.9%), while was similar to the check cultivar 'Shinyoung' in acid detergent fiber (33.9%), neutral detergent fiber (57.3%), and total digestible nutrients (62.2%). It showed a grain yield of $5.59MT\;ha^{-1}$, which was 38% higher than that of the check cultivar 'Shinyoung' ($4.05MT\;ha^{-1}$). This cultivar is recommended for fall sowing forage crops in areas in which average daily minimum mean temperatures in January are higher than $-10^{\circ}C$.

Short Bowel Syndrome as the Leading Cause of Intestinal Failure in Early Life: Some Insights into the Management

  • Goulet, Olivier;Nader, Elie Abi;Pigneur, Benedicte;Lambe, Cecile
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.22 no.4
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    • pp.303-329
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    • 2019
  • Intestinal failure (IF) is the critical reduction of the gut mass or its function below the minimum needed to absorb nutrients and fluids required for adequate growth in children. Severe IF requires parenteral nutrition (PN). Pediatric IF is most commonly due to congenital or neonatal intestinal diseases or malformations divided into 3 groups: 1) reduced intestinal length and consequently reduced absorptive surface, such as in short bowel syndrome (SBS) or extensive aganglionosis; 2) abnormal development of the intestinal mucosa such as congenital diseases of enterocyte development; 3) extensive motility dysfunction such as chronic intestinal pseudo-obstruction syndromes. The leading cause of IF in childhood is the SBS. In clinical practice the degree of IF may be indirectly measured by the level of PN required for normal or catch up growth. Other indicators such as serum citrulline have not proven to be highly reliable prognostic factors in children. The last decades have allowed the development of highly sophisticated nutrient solutions consisting of optimal combinations of macronutrients and micronutrients as well as guidelines, promoting PN as a safe and efficient feeding technique. However, IF that requires long-term PN may be associated with various complications including infections, growth failure, metabolic disorders, and bone disease. IF Associated Liver Disease may be a limiting factor. However, changes in the global management of IF pediatric patients, especially since the setup of intestinal rehabilitation centres did change the prognosis thus limiting "nutritional failure" which is considered as a major indication for intestinal transplantation (ITx) or combined liver-ITx.

Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms

  • Fu, Yeqi;Liu, Yunqiu;Abuzeid, Asmaa M.I.;Huang, Yue;Zhou, Xue;He, Long;Zhao, Qi;Li, Xiu;Liu, Jumei;Ran, Rongkun;Li, Guoqing
    • Parasites, Hosts and Diseases
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    • v.57 no.1
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    • pp.9-15
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    • 2019
  • Melting temperature shift ($T_m-shift$) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a $T_m-shift$ method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5' end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of $T_m-shift$ was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The $T_m-shift$ method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of $T_m$- values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was $5.22{\times}10^{-6}$ and $5.28{\times}10^{-6}ng/{\mu}l$ samples of AceP and AtuP, respectively. The accuracy of $T_m-shift$ method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the $T_m-shift$ detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.

Surgical Treatment of Chronic Tophaceous Gout in the 1st Metatarso-Phalangeal Joint (족부 제 1중족 족지 관절에 발생한 만성 결절성 통풍의 수술적 치료)

  • Lee, Tae-Hun;Nam, Il-Hyun;Ahn, Gil-Yeong;Lee, Yeong-Hyeon;Lee, Yong-Sik;Choi, Young-Deuk;Lee, Hee-Hyung
    • Journal of Korean Foot and Ankle Society
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    • v.22 no.4
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    • pp.156-160
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    • 2018
  • Purpose: Chronic tophaceous gout is a painful and disabling inflammatory disease. Surgical treatment for chronic tophaceous gout is very difficult with many complications. This study evaluated the efficacy of shortening scarf osteotomy on the treatment of chronic tophaceous gout in the 1st metatarso-phalangeal (MTP) joint. Materials and Methods: From January 2006 to December 2015, 14 patients (19 cases) who underwent axial shortening scarf osteotomy for chronic tophaceous gout were reviewed. All patients were male. The average age at the time of surgery was 59.6 years (42~66 years). The minimum follow-up was 24 months. Total removal of the tophi mass with the adhered medial capsule of the 1st MTP joint was attempted. Axial shortening scarf osteotomy was done on the 1st metatarsal shaft. The visual analogue scale (VAS) for pain and the American Orthopaedic Foot and Ankle Society (AOFAS) forefoot score was assessed preoperatively and postoperatively. The range of motion (ROM) of the 1st MTP joint was also compared pre- and postoperatively. Results: The average size of the extracted tophaceous mass was 32 mm. The mean amount of the length of metatarsal shortening was 4.9 mm. The mean ROM of the 1st MTP joint was improved from $30.4^{\circ}$ to $62.3^{\circ}$. The mean AOFAS forefoot score improved from 51.4 to 86.6 points. The mean VAS for pain improved from 4.6 to 0.3 points. Conclusion: The axial shortening scarf osteotomy used on chronic tophaceous gout could reconstruct the 1st MTP joint with an improved ROM and was free of pain. Axial shortening scarf osteotomy is suggested as a useful and effective method for the treatment of chronic tophaceous gout.

A Study on Safe Separation Distance between Tunnel and Interchange (터널과 입체 교차로간의 안전한 이격거리 연구)

  • Lee, In-Bae;Kim, Nakseok
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.39 no.2
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    • pp.273-279
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    • 2019
  • Development of mountain area is increasing due to the demand for improvement of traffic convenience and development of underdeveloped area. Therefore, there frequently are sections where tunnels and interchanges are located close to each other. These sections do not only affect tunnel planning, types and length of interchanges, but also affect more on route selection. In Korea, several design criteria present each reference value but these values are very similar. In the situation, the minimum value among them is usually applied when planning roads and it could cause traffic safety problems in different site conditions. In this study, the problems of design speed, illuminance adaptation distance, and lane change intervals are analyzed by simulating the cases that the problem could occur when calculating the separation distance between tunnel and interchange. The results obtained from this study can be summarized as following: the driving speed should be applied in case that the site has a big gap between design speed and driving speed because the uniform application of the design speed is not safe; the illuminance adaptation distance should include the influence distance in the section affected by the direct light; in addition, the lane change distance should include the time to perceive the situation of the next lane after the lane change in the section required for successive lane change.

Capacity Design of Eccentrically Braced Frame Using Multiobjective Optimization Technique (다목적 최적화 기법을 이용한 편심가새골조의 역량설계)

  • Hong, Yun-Su;Yu, Eunjong
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.33 no.6
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    • pp.419-426
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    • 2020
  • The structural design of the steel eccentrically braced frame (EBF) was developed and analyzed in this study through multiobjective optimization (MOO). For the optimal design, NSGA-II which is one of the genetic algorithms was utilized. The amount of structure and interfloor displacement were selected as the objective functions of the MOO. The constraints include strength ratio and rotation angle of the link, which are required by structural standards and have forms of the penalty function such that the values of the objective functions increase drastically when a condition is violated. The regulations in the code provision for the EBF system are based on the concept of capacity design, that is, only the link members are allowed to yield, whereas the remaining members are intended to withstand the member forces within their elastic ranges. However, although the pareto front obtained from MOO satisfies the regulations in the code provision, the actual nonlinear behavior shows that the plastic deformation is concentrated in the link member of a certain story, resulting in the formation of a soft story, which violates the capacity design concept in the design code. To address this problem, another constraint based on the Eurocode was added to ensure that the maximum values of the shear overstrength factors of all links did not exceed 1.25 times the minimum values. When this constraint was added, it was observed that the resulting pareto front complied with both the design regulations and capacity design concept. Ratios of the link length to beam span ranged from 10% to 14%, which was within the category of shear links. The overall design is dominated by the constraint on the link's overstrength factor ratio. Design characteristics required by the design code, such as interstory drift and member strength ratios, were conservatively compared to the allowable values.