• Title/Summary/Keyword: mineral oil inoculation method

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Screening of Disease Resistance of Chinese Cabbage Cultivars and Lines to Bacterial Soft Rot (배추 무름병에 대한 저항성 품종 검정)

  • Chung, Eun-Kyoung;Zhang, Xuan-Zhe;Choi, Bo-Ra;Lee, Eun-Ju;Yeoung, Young-Rog;Kim, Byung-Sup
    • Research in Plant Disease
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    • v.9 no.1
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    • pp.39-41
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    • 2003
  • Bacterial soft rot by Erwinia carotovora subsp. carotovora is one of the diseases causing the biggest damages in Chinese cabbage cultivation. This study was conducted to evaluate disease resistance of Chinese cabbage cultivars and breeding lines to E. carotovora subsp. carotovora by new inoculation method, mineral oil inoculation method, inoculating 10 ml of the mixture (4:1, v/v) of bacterial suspension and mineral oil on the central bases of Chinease cabbage seedling. Total 43 Chinese cabbage cultivars and lines obtained from 3 domestic seed companies and universities were screened for disease resistance using the above mentioned inoculation method. This screening test showed that Chinese cabbage C3-26, C3-28, C3-29 and C29-51-51-53 lines were resistant, Gangta, Gumchonyealgali, Mini, DB50, Jibu, Pyungchng, Sanchon and Yellow King No.2 cultivars were susceptible, and the others were moderate resistant.

Suppression of Bacterial Soft Rot on Chinese Cabbage by Calcium Fertilizer Treatment (칼슘비료 처리에 의한 배추 무름병 발생 억제)

  • Kim, Byung-Sup;Yeoung, Young-Rog
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.82-85
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    • 2004
  • Bacterial soft rot by Erwinia carotovora subsp. carotovora is one of the diseases causing the biggest damages in Chinese cabbage cultivation. This study was conducted to evaluate on suppressive effect of calcium fertilizer to bacterial soft rot of Chinese cabbage. Seven calcium fertilizers were selected for evaluation. And screening was conducted to select effective agents for controlling bacterial soft rot. When applied by the nursery test condition using mineral oil inoculation method with Chinese cabbage, calcium hydroxide had more suppressive efficacy than any other calcium fertilizer, While nitrogen fertilizer was induced the disease, calcium hydroxide was suppressed soft rot disease in field test as well as seedling test. Treatment of calcium+nitrogen fertilizer as well as calcium only showed a significant control effect in the field experiment with Chinese cabbage 'Sanchon' in 2003.

Screening of effective control agents against bacterial soft rot on Chinese cabbage in alpine area (고랭지 배추 무름병 방제를 위한 우수약제 선발)

  • Chung, Eun-Kyoung;Zhang, Xuan-Zhe;Yeoung, Young-Rog;Kim, Byung-Sup
    • The Korean Journal of Pesticide Science
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    • v.7 no.1
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    • pp.32-37
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    • 2003
  • Bacterial soft rot by Erwinia carotovora subsp. carotovora is one of the diseases causing the biggest problem in Chinese cabbage. Chemical screening was conducted to select effective agents for controlling bacterial soft rot. Control effect of antibiotics, plant activator, and Biokeeper (avirulent Erwinia) to soft rot were tested by in vitro assay, nursery test, and field experiment. The in vitro assay was done by paper disc method and potato slice method. The nursery test was performed by using mineral oil inoculation method with consistent disease induction. The in vitro assay showed that streptomycin, oxolinic acid, bronopol, and copper hydroxide significantly suppressed the growth of pathogenic bacterium and the decomposition of potato slice. However, plant activators including acibenzolar-S-methyl did not show the suppressive effect on the growth of pathogenic bacterium and the decomposition of potato slice. When applied by the nursery test condition using mineral oil inoculation method with Chinese cabbage 'Kangruckyeurum', Biokeeper, oxolinic acid, antibiotics streptomycin, validamycin, and copper compound provided 83.5%, 95.2%, 91.2%, 57.5% and 79.9% in control efficacy, respectively. However, the control effect of acibenzolar-S-methyl showed to be low to cause phytotoxicity. Also acibenzolar-S-methyl showed a significant control effect in the field experiment with Chinese cabbage 'Sanchon' in 2000, but the field experiment with Chinese cabbage 'Kangruckyeurum' in 2001 revealed it had phytotoxicity to Chinese cabbage. Such a difference was considered to be caused by differences in phytotoxic reaction of Chinese cabbage cultivars to the chemical. Streptomycin+copper, copper hydroxide and Biokeeper showed 79.7%, 71.9% and 60.9% in control efficacy, respectively, in the field experiment with Chinese cabbage 'Sanchon' in 2002.