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Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography

  • Xie, Wen-Ming;Ko, Kwang-Yong;Kim, Sung-Hun;Chang, Hee-Ra;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.25 no.4
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    • pp.359-364
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    • 2006
  • Reversed-phase high-performance liquid chromatography (HPLC) techniques were developed to quantify abamectin (ABM) in paprika (Capsicum annum). Separation was achieved on a $C_{18}$ ODS column with a mobile phase of acetonitrile/water (96/4, v/v) mixture in an isocratic elution at the flow tate of 1.2 mL/min for avermectins (AVMs). The retention times were 8.0 and 9.7mins for AVM $B_{lb}$ and AVM $B_{1a}$, respectively. Residual AVMs (sum of AVM $B_{1a}$, AVM $B_{1b}$ and 8,9-Z-AVM $B_{1a}$) in the vegetable were extracted with acetonitrile, and the silica solid-phase extraction cartridges were used to purify the extract. AVMs were derivatized using trifluoroacetic acid and 1-methylimidazole, and the derivatives were determined with a fluorescence detector (excitation at 365 nm and emission at 470 nm). High and consistent recoveries, ranging from 93% to 115%, were obtained for AVM $B_{1a}$ and 8, 9-Z-AVM $B_{1a}$ at fortified levels of $20{\mu}g/kg\;and\;200{\mu}g/kg$ for paprika. The limit of quantitation (LOQ) was $2{\mu}g/kg$. The residual levels of AVMs in paprika in a field experiment from one day to seven days after the last application decreased from 18.40 to $7.59{\mu}g/kg$. The half-life $(T_{1/2})$ of AVMs in paprika was 1.47 days.

Biosorption and Desorption of Pb by using Sargassum horneri (괭생이모자반, Sargassum horneri를 이용한 Pb 흡착 및 탈착)

  • SUH Kuen-Hack;AHN Kab-Hwan;KIM Byong-Jin;CHO Jin-Koo;JIN Hyung-Joo;HONH Yong-Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.3
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    • pp.333-337
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    • 1999
  • Biosorption of Pb to Sargassum horneri was evaluated. An adsorption equilibrium was reached in about 15 min. for Pb and Pb uptake was 185.5 mg Pb/g biomass. When Ca and Mg concentration increases in Pb solution, Pb was selectively adsorbed. The Pb adsorbed by S. horneri could be recovered by desorption process, and the desorption efficiency of Pb by 0.1 M HCl, 0.1 M $HNO_3$, and 0.1 M EDTA was above $95\%$, whereas the elution by 0.1 M NaOH was less efficient in the desorption of Pb. S. horneri was reused 6 cycle repeatedly after adsorption/desorption and the sum of uptake was 636.6 mg Pb/g biomass.

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Image Retrieval using Local Color Histogram and Shape Feature (지역별 색상 분포 히스토그램과 모양 특징을 이용한 영상 검색)

  • 정길선;김성만;이양원
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 1999.05a
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    • pp.50-54
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    • 1999
  • This paper is proposed to image retrieval system using color and shape feature. Color feature used to four maximum value feature among the maximum value extracted from local color distribution histogram. The preprocessing of shape feature consist of edge extraction and weight central point extraction and angular sampling. The sum of distance from weight central point to contour and variation and max/min used to shape feature. The similarity is estimated compare feature of query image with the feature of images in database and the candidate of image is retrieved in order of similarity. We evaluate the effectiveness of shape feature and color feature in experiment used to two hundred of the closed image. The Recall and the Precision is each 0.72 and 0.53 in the result of average experiment. So the proposed method is presented useful method.

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Relationships Between Cadmium, Copper, Mercury, Zinc Levels and Metallothionein in the Liver and Kidney Cortex of Korean (한국인 간장 및 신장피질에 함유된 카드뮴, 구리, 수은, 아연 함량과 메탈로치오나인과의 관계)

  • Lee Sang Ki;Yoo Young Chan;Yun Yeo Pyo;Yang Ja YouL;Oh Seung Min;Chung Kyu Hyuck
    • Environmental Analysis Health and Toxicology
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    • v.19 no.4
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    • pp.383-388
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    • 2004
  • In order to elucidate the relationships between cadmium, copper, mercury, zinc levels and metallothionein in the liver and kidney cortex of Korean, the levels of Cd, Zn, Hg, Cu and metallothionein (MT) were determined in the kidney cortex and liver of 50 subjects deceased in the period of January-November, 2001 in the area of Seoul and Gyeonggi Province of Korea. The mean age of the population studied was 36.3+/-12.3 years. The tissues were digested with microwave digestion system and the elements were determined by inductively coupled plasma atomic emission spectrometry. MT was determined by the Cd-hemoglobin affinity assay. The determined levels (mean+/-SD) were: 33.9+/-18.9 micrograms Cd/g wet weight; 47.5+/-12.6 micrograms Zn/g wet weight; 2.5+/-0.57 microgram Cu/g wet weight; 0.26+/-0.31 micrograms Hg/g wet weight, 4.0+/-3.1 mg MT/g wet weight in renal cortex and 2.5+/-1.9 micrograms Cd/g wet weight; 46.9+/-15.0 micrograms Zn/g wet weight; 6.2+/-2.5 micrograms Cu/g wet weight; 0.10+/-0.15 micrograms Hg/g wet weight, 0.92+/-0.57 mg MT/g wet weight in the liver. Positive relationships between Cd and MT, sum of four divalent metal and MT in the kidney cortex were observed. No other correlation was found between Cu and MT, Hg and MT, Zn and MT in either organs.

Design of a High Performance 32$\times$32-bit Multiplier Based on Novel Compound Mode Logic and Sign Select Booth Encoder (새로운 복합모드로직과 사인선택 Booth 인코더를 이용한 고성능 32$\times$32-bit 곱셈기의 설계)

  • Kim, Jin-Hwa;Song, Min-Gyu
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.38 no.3
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    • pp.205-210
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    • 2001
  • In this paper, a novel compound mode logic based on the advantage of both CMOS logic and pass-transistor logic(PTL) is proposed. From the experimental results, the power-delay products of the compound mode logic is about 22% lower than that of the conventional CMOS logic, when we design a full adder. With the proposed logic, a high performance 32$\times$32-bit multiplier has been fabricated with 0.6um CMOS technology. It is composed of an improved sign select Booth encoder, an efficient data compressor based on the compound mode logic, and a 64-bit conditional sum adder with separated carry generation block. The Proposed 32$\times$32-bit multiplier is composed of 28,732 transistors with an active area of 1.59$\times$1.68 mm2 except for the testing circuits. From the measured results, the multiplication time of the 32$\times$32-bit multiplier is 9.8㎱ at a 3.3V power supply, and it consumes about 186㎽ at 100MHz.

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Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

  • Jeong, Ji-Sun;Lee, Jonghae;Pak, Yong-Nam
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3817-3824
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    • 2013
  • Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM10

  • Kim, Hyun-Jin;Yoon, Seok-Jun;Kim, Hyeong-Su;Lee, Kun-Sei;Kim, Eun-Jung;Jo, Min-Woo;Oh, In-Hwan
    • Journal of Environmental Health Sciences
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    • v.37 no.5
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    • pp.387-395
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    • 2011
  • Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

Evaluation of Water Quality Characteristics and Water Quality Improvement Grade Classification of Geumho River Tributaries (금호강 수계 지류하천의 수질 특성 평가 및 수질개선 등급화 방안)

  • Jung, Kang-Young;Ahn, Jung-Min;Kim, KyoSik;Lee, In Jung;Yang, Duk Seok
    • Journal of Environmental Science International
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    • v.25 no.6
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    • pp.767-787
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    • 2016
  • In this study, we analyzed on-site monitoring data for 15 tributaries in Geumho watersheds for 3 years (2011-2013) in order to sort out priorities on water quality characteristics and improvement. As a result of estimating contribution to contamination of the tributary rivers, Dalseocheon showed the highest load densities, despite the smallest watershed area, with 22.7% $BOD_5$, 30.7% $COD_{Mn}$, 31.3% TOC and 47.6% TP. After conducting PCA (principal component analysis) and FA (factor analysis) to analyze water quality characteristics of the tributary rivers, the first factor was classified as $COD_{Mn}$, TOC, EC, TP and $BOD_5$, the second factor as pH, Chl-a and DO, the third factor as water temperature and TN, and the fourth factor as SS and surface flow. In addition, arithmetical sum of each factor's scores based on grading criteria revealed that Dalseocheon and Namcheon were classified into Group A for their highest scores - 96 and 93, respectively -, and selected as rivers that require water environmental management measures the most. Also, water environmental contamination inspection showed that Palgeocheon had the most number of aquatic factors to be controlled: $BOD_5$, $COD_{Mn}$, SS, TOC, T-P, Chl-a, etc.

Study on the Satisfaction Use of Rehabilitation Exercise Room (일부 보건소의 재활운동실 이용 만족도에 관한 연구)

  • Kwon Seon Suk;Kwon Dong Youn
    • Journal of Korean Public Health Nursing
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    • v.17 no.2
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    • pp.203-211
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    • 2003
  • The purpose of this study was to investigate the degree of satisfaction in using rehabilitation exercise rooms in public health centers. A total of 100 subjects were participated in the study who were clients of rehabilitation exercise rooms in two public health centers in one city of Kyunggi Do, Korea. Data were collected from March 1 to 30, 2003 using questionnaire. The data were analyzed using SPSS/PC computer program. The result are as follows : 1. The mean age of subjects was 62.6 years old, and men constituted $67.0\%$ of the participants. Most participants $(64\%)$ were in the middle socioeconomic status, and $(65\%)$ of subjects was diagnosed as stroke. 2. Regarding the source of information about rehabilitation exercise room in public health center, $56.0\%$ obtain information from colleagues or neighbors, and for most participants the time required to travel to rehabilitation exercise room was less than 30min on foot. Among rehabilitation services, Taping therapy $(53.0\%)$ was most frequently used among participants, 3. Sum of all satisfaction score was $71.10(\pm6.60,\;range:\;1-90)$ showed higher level of satisfaction. Cost, reliability, and kindness were main factors that affect the degree of satisfaction among participants. 4. Twenty-four percent of the respondents utilized clinic services in the health centers, and $27.4\%$ stoped rehabilitation services from hospitals and/or clinics due to high cost. 5. In the use of rehabilitation exercise room was positively correlated to age (r=.214, p<0.05) and disease duration(r=.241. p<0.05).

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Feedback Reduction Scheme of SDMA with Quantized CSI using User Restriction (사용자 제한을 이용한 양자화된 채널 상태 정보를 갖는 공간 분할 다중 접속 방식의 되먹임 감소 기법)

  • Seo, Woo-Hyun;Park, Sung-Soo;Min, Hyun-Kee;Hong, Dea-Sik
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.47 no.2
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    • pp.25-33
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    • 2010
  • Introducing the quantized channel state information (CSI), space division multiple access (SDMA) can extract the multiplexing gain with the limited feedback burden. However, huge signaling burden of feedback can still suffer SDMA system because the total feedback data of SDMA is linearly dependent on the number of users. Hence, we propose a new feedback scheme to control the feedback load decided by the number of users. In this scheme, the cut-off level, which restricts the feedbacks of poor conditioned users, is suggested for the reduction of the feedback burden without the performance loss. From simulation results, then, we show that the proposed feedback scheme can achieve not only the sum-rate gain but also the reasonable feedback reduction.