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Effects of He-Ne Laser Irradiation on the Activity of the Cultured Fibroblast (He-Ne 레이저 조사가 배양 섬유모세포의 활성에 미치는 영향)

  • Song In-Young;Lee Jae-Hyoung
    • The Journal of Korean Physical Therapy
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    • v.9 no.1
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    • pp.71-79
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    • 1997
  • The purpose of this study were to determined the effect of laser irradiation on the fibroblast activity. Cultures of 3T3 fibroblasts were subjected to Helium Neon laser(632.8 nm) irradiation of various energy density. On one, two and three consecutive days the fibroblast monolayers wert irradiated for period from 0 to 32 minutes with 8 mW of average output power. The fibroblast activity was determined by the quantitative assay of MTT, SRB and NR after incubation of the fibroblasts for 24 hours. Results show that exposure duration from 2 min to 32 min could increase MTT at three consecutive days, whereas control and 1 min, one and two days irradiation had were not inclosed. The SRB and NR were inclosed at two and three consecutive days from 2 min to 32 min, whereas control and 1 min, and once radiation were not increased. These result demonstrate that energy density from 0.48 to 7.64 J/cm could increase cellular protein contents and fibroblast activity at more than twice irradiation of laser, whereas low energy density (less than 0.24 J/m) and once irradiation of laser had no effect. The results suggest that the beneficial effect of the He-Ne laser with adequate dose on fibroblast activity in vitro.

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Si Based Photoelectric Device with ITO/AZO Double Layer (ITO/AZO 투명전극을 이용한 Si 기반의 광전소자)

  • Jang, Hee-Joon;Yoon, Han-Joon;Lee, Gyeong-Nam;Kim, Joondong
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.31 no.2
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    • pp.85-89
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    • 2018
  • In this study, functional transparent conducting layers were investigated for Si-based photoelectric applications. Double transparent conductive oxide (TCO) films were deposited on a Si substrate in the sequence of indium tin oxide (ITO) followed by aluminum-doped zinc oxide (AZO). First, we observed that the conductivity and transparency of AZO dominate the overall performance of the double TCO layers. Secondly, the double layered TCO film (consisting of AZO/ITO) deposited by sputtering was compared to a AZO-only film in terms of their optical and electrical properties. We prepared three different AZO films: ITO:3min/AZO:10min, ITO:5min/AZO:7min, and ITO:7min/AZO:4min. The results show that the optical properties (transmittance, absorbance, and reflection) can be controlled by the film composition. This may provide a significant pathway for the manipulation of the optical and electrical properties of photoelectric devices.

Quantitative analysis of Spirulina platensis growth with CO2 mixed aeration

  • Kim, Yong Sang;Lee, Sang-Hun
    • Environmental Engineering Research
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    • v.23 no.2
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    • pp.216-222
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    • 2018
  • The growth characteristics of Spirulina platensis were investigated using four photo-bioreactors with $CO_2$-mixed air flows. Each reactor was operated under a specific condition: 3% $CO_2$ at 50 mL/min, 3% $CO_2$ at 150 mL/min, 6% $CO_2$ at 50 mL/min, and 6% CO2 at 150 mL/min. The 3% $CO_2$ at 150 mL/min condition produced the highest algal growth rate, while the 6% $CO_2$ at 150 mL/min conditioned produced the lowest. The algal growth performance was suitably assessed by the linear growth curve rather than the exponential growth. The medium pH decreased from 9.5 to 8.7-8.8 (3% $CO_2$) and 8.4-8.5 (6% $CO_2$), of which trends were predicted only by the pH-carbonate equilibrium and the reaction kinetics between dissolved $CO_2$ and $HCO_3{^-}$. Based on the stoichiometry between the nutrient amounts and cell elements, it was predicted that depleted nitrogen (N) at the early stage of the cultivation would reduce the algal growth rates due to nutrient starvation. In this study, use of the photobioreactors capable of good light energy distribution, proper ranges of $CO_2$ in bubbles and medium pH facilitated production of high amounts of algal biomass despite N limitation.

Physiological Changes in Jeju Crossbred Riding Horses by Swim Training

  • Kang, Ok-Deuk;Ryu, Youn-Chul;Yun, Young-Min;Kang, Min-Soo
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.2
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    • pp.200-206
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    • 2012
  • The changes in physiologic parameters by swim exercise duration were examined in five female well-trained Jeju crossbred riding horses that had riding experience of more than three years without swim training experience. The horses were performed with swim exercise for 10 min (60.0 m/min) once a day for 14 days. Physiologic characteristics and haematic parameters were measured before swimming, immediately after swimming, and after a 10 min rest at first day ($D_0$), 7 days ($D_7$), and 14 days ($D_{14}$) of training. After 14 days of swim training, heart rate (p<0.05), blood glucose (p<0.05), lactate concentration (p<0.001), packed cell volume (p<0.01), and hemoglobin (p<0.01) measured immediately after swim and after 10 min rest showed significant lower values than those of $D_0$. The results illustrate the benefits of swim training for riding horses and the need for the establishment of swimming routines of appropriate duration and intensity to maximize the advantages of swim training.

The Physicochemical Properties of $\alpha$-Amylase Inhibitors from Black Bean and Naked Barey in Korea (한국산 검정콩 및 쌀보리 $\alpha$-Amylase 저해물질의 이화학적 특성)

  • 심기환;문주석;배영일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.3
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    • pp.367-375
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    • 1998
  • The physicochemical properties of the $\alpha$-amylase inhibitors from black bean and naked barley is Korea were investigated. Preincubation time for maximum inhibition was 30min and no activity change was seen after that time. Optimum pH of the $\alpha$-amylase inhibitors from the black bean and naked barley was pH 7.0 and the inhibitory activities were stable in the range of pH 6.0~8.0 in both phosphate and Tris-HCI buffer solutions. Both inhibitors maintained more than 50% of activity after incubation for 17 min at 7$0^{\circ}C$. The inhibitors from the black bean and naked barley maintained more than 50% of activities after treatment for 40 min and 30 min with pepsin, and 30 min and 50 min with trypsin, respectively. Both inhibitors functioned via a noncompetitive mechanism and were active against porcine pancreatic and human salivary $\alpha$-amylases. The activities of both inhibitors were linear for the ionic stength ranging from 0 to 0.9. The addition of 70 mM maltose to the reaction mixture caused a maximum increase in the relative activities of both inhibitors, but it did not affect the dissociation of the EI complex. The activities of both inhibitors were significantly enhanced by adding 1mM of K+ or Mg2+.

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A Study on the Short Break Time on VDT Work using EMG (근전도를 이용한 VDT 작업시 짧은 휴식시간에 관한 연구)

  • Kim, Yu-Chang;Lee, Jun-Pal
    • Journal of the Ergonomics Society of Korea
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    • v.26 no.4
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    • pp.41-47
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    • 2007
  • This paper suggested the best work conditions including short break time and the number of mouse clicks on the computer work for the prevention of MSDs on VDT work. Fatigue measures included EMG based parameters. The short break time conditions are grouped into 7, 15, and 30 seconds after every work for 10 min and the number of mouse clicks are divided into 10, 20, and 30 clicks/min. The result of the ANOVA of the shift value of %MVC(Maximum Voluntary Contraction) showed the following: 1) There was a considerable difference as regards to the break time except the number of mouse clicks on the upper trapezius muscle(p$<$0.05). The best conditions were shown in 15 sec after every 10 min and 30 clicks/min. 2) There were considerable differences as regards to the number of mouse clicks except the break time on the extensor digitorum muscle and extensor carpi ulnaris muscle(p$<$0.05). The best conditions were shown in 7 sec after every 10min and 10 clicks/min.

A Study on Tensile Properties and HAZ Softening Depending on the Amount of Heat Input in MIG Welding of Al6082-T6 (Al6082-T6의 MIG용접부에서 입열량에 따른 열영향부의 연화와 인장특성에 관한 연구)

  • Baek, Sang-Yeob;Park, Kyung-Do;Kim, Won-Il;Cho, Sang-Myung
    • Journal of Welding and Joining
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    • v.29 no.1
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    • pp.59-64
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    • 2011
  • Al6082-T6 is widely used because of its corrosion resistance and excellent strength. HAZ softening occurs in MIG welding process for this aluminium alloys because this aluminium alloy is heated to higher temperature than its aging temperature during welding. Therefore, low heat input and minimum standard deviation of heat input are required for narrow HAZ width and, for higher strength of welds. In this study, Al6082-T6 was used to examine for HAZ softening with various heat input in aluminium MIG welding. For weldments, micro hardness was measured and tensile test was carried out. Minimum hardness was increased at high speed welding such as 80cm/min and 120cm/min in welding speed comparing with 40cm/min. Also, in case of high speed welding such as 80cm/min and 120cm/min, tensile strength of weldments was increased about 10% comparing with low speed welding(40cm/min).

Monitoring of petroleum hydrocarbon degradative potential of indigenous microorganisms in ozonated soil

  • ;;Rameshwar;Tatavarty
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.152-157
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    • 2003
  • Diesel-contaminated soils were ozonated for different times (0 - 900 min) and incubated for 9 wk to monitor petroleum hydrocarbons (PH)-degradative potential of indigenous microorganisms in the soils. Increased ozonation time decreased not only concentration of PH but also number of microorganisms in the soils. Microorganisms in the ozonated soils increased during 9-wk incubation as monitored by culture- and nonculture-based methods. Higher (1-2 orders of magnitude) cell number was observed by quantitative analysis of soil DNA using probes detecting genes encoding 165 rRNA(rrn), naphthalene dioxygenase (nahA), toluene dioxygenase (todC), and alkane hydroxylase (alkB) than microbial abundance estimated by culture-based methods. Such PH-degraders were relatively a few or under detection limit in 900-min ozonated soil. Further PH-removal observed during the incubation period supported the presence of PH-degraders in ozonated soils. Highest reduction (25.4%) of total PH (TPH) was observed in 180-min ozonated soil white negligible reduction was shown in 900-min ozonated soil during the period, resulting in lowest TPH-concentration in 180-min ozonated soil among the ozonated soils. Microbial community composition in 9-wk incubated soils revealed slight difference between 900-min ozonated and unozonated soils as analyzed by whole cell hybridization using group-specific rRNA-targeted oligonucleotides. Results of this study suggest that appropriate ozonation and subsequent biodegradation by indigenous microorganisms may be a cost-effective and successful remediation strategy for PH-contaminated soils.

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현장 규모 biobarrier의 수리학적 특성과 기초 설계

  • 최영화;오재일;왕수균;배범한
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.09a
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    • pp.426-430
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    • 2003
  • Subsurface biobarrier technology has potential applications to contain contaminated groundwater and/or to degrade toxic pollutants in groundwater. Effective biobarrier formation is need to assess of hydrogeologic characteristics and to conduct practical operation strategies and design based on this prior to design biobarrier. Thus, in this study, we examined hydrogeologic characteristics in biobarrier construction site. Hydraulic conductivities which calculated from slug test data have shown difference with each well as 1.20$\times$10$^{-3}$ -6.00$\times$10$^{-5}$ cm/sec. Tracer test is a method in which concentration of tracer solution during withdrawal in each well by vacuum extraction system is measured with time. Tracer solution was continuously injected by constant head tank. Measured tracer concentration versus time data were fitted to analytical solution of convection dispersion equation (CDE). The fitting data of CDE to the measured data at each extraction well yielded were 0.61cm/min(pore velocity), 5.38$\textrm{cm}^2$/min(dispersion coefficient) for discharge rate of 0.47 1/min and 1.75cm/min(pore velocity), 36.34$\textrm{cm}^2$/min(dispersion coefficient) for discharge rate of 0.93 1/min. As a result, we acquired fundamental parameters which need to design biobarrier and operation strategies.

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Removal of Taste and Odor by Powdered Activated Carbon Adsorption and Air Stripping (분말활성탄 흡착 및 탈기에 의한 이취미 제거)

  • Jun, Hang-Bae;Na, Kwang-Ju;Seo, Tae-Kyeong;Park, Sang-Min
    • Journal of Korean Society of Water and Wastewater
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    • v.22 no.4
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    • pp.455-460
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    • 2008
  • Powered activated carbon(PAC) has been widely applied for controling odor causing compounds(OCCs) from water treatment plants. Because of their volatility, the OCCs can also be removed from water by air stripping methods. In this study, OCCs removal was tested with PAC adsorption, air stripping, and both PAC adsorption and air stripping from the Taecheong lake water. Removal efficiency of OCCs in terms of threshold odor number(TON) were 39.6% by both PAC (15mg/L) adsorption and aeration for 30 min, 33.6% by PAC(15mg/L) adsorption alone for 30 min, and 22.9% by aeration alone for 30 min, respectively. OCCs could be removed up to 50% by aeration for 120 min without PAC adsorption. At an extended aeration with 15mg/L of PAC, OCCs removal occurred mainly by PAC adsorption within 30 min aeration while it continued by air stripping afterward. At simulated jar tests with the raw water, removal efficiencies of geosmin and MIB were 48.3, 36.1% by coagulation and sedimentation without PAC addition. With 15mg/L of PAC on the same jar tests, the removal efficiencies were 83.1, 60.1%, respectively. Without PAC, OCCs could be possibly removed by stripping during the agitation processes.