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Effect of butanol fraction from Cassia tora L. seeds on glycemic control and insulin secretion in diabetic rats

  • Nam, Jeong-Su;Choi, Hyun-Ju
    • Nutrition Research and Practice
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    • v.2 no.4
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    • pp.240-246
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    • 2008
  • Cassia tora L. seeds have previously been reported to reduce blood glucose level in human and animals with diabetes. In the present study, the effects of Cassia tora L. seed butanol fraction (CATO) were studied on postprandial glucose control and insulin secretion from the pancreas of the normal and diabetic rats. Diabetes was induced by an i.p. injection of Streptozotocin (55 mg/kg BW) into the male Sprague-Dawley rats. The postprandial glucose control was monitored during a 240 min-period using a maltose loading test. In normal rats, rats fed CATO (20 mg/l00 g BW/d) showed lower postprandial glucose levels in all the levels from 30 min up to 180 min than those in the control rats without CATO (p<0.05). In diabetic rats, those levels in the CATO group seemed to be lower during the $30{\sim}180$ min, but only glucose level at 30 min showed significant difference compared to that in the control group. Moreover, CATO delayed the peak time of the glucose rise in both normal and diabetic rats in the glucose curves. On the other hand, when CATO was administered orally to the diabetic rats for 5 days, 12 hr fasting serum glucose level was decreased in the diabetic rats (p<0.05). Degree of a decrease in 12 hr fasting serum insulin levels was significantly less in the diabetic CATO rats as compared to diabetic control rats. On the last day of feeding, P cells of the pancreas were stimulated by 200 mg/dL glucose through a 40 min-pancreas perfusion. Amounts of the insulin secreted from the pancreas during the first phase ($11{\sim}20$ min) and the second phase ($21{\sim}40$ min) in the CATO fed diabetic rats were significantly greater than those in the diabetic control group (p<0.05). These findings indicated that constituents of Cassia tora L. seeds have beneficial effect on postprandial blood glucose control which may be partially mediated by stimulated insulin secretion from the pancreas of the diabetic rats.

Formation Behavior of Anodic Oxide Films on Al 6061 Alloy in Sulfuric Acid Solution (황산 용액에서 Al6061 합금의 아노다이징 피막 형성거동)

  • Moon, Sungmo;Jeong, Kihun;Lim, Sugun
    • Journal of Surface Science and Engineering
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    • v.51 no.6
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    • pp.393-399
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    • 2018
  • Formation behavior of aluminum anodic oxide (AAO) films on Al6061 alloy was studied in view of thickness, morphology and defects in the anodic films in 20 vol.% sulfuric acid solution at a constant current density of $40mA/cm^2$, using voltage-time curve, observation of anodized specimen colors and surface and cross-sectional morphologies of anodic films with anodization time. With increasing anodizing time, voltage for film formation increased exponentially after about 12 min and its increasing rate decreased after 25 min, followed by a rapid decrease of the voltage after about 28 min. Surface color of anodized specimen became darker with increasing anodizing time up to about 20 min, while it appeared to be brighter with increasing anodizing time after 20 min. The darkened and brightened surfaces with anodizing time are attributed to an increase in thickness of porous anodic oxide film and a chemical damage of the films due to heat generated by increased resistance of the film, respectively. Cross-sectional observation of AAO films revealed the formation of defects of crack shape at the metal/oxide interface after 15 min which prevents the growth of AAO films. Width and length of the crack-like defect increased with anodizing time up to 25 min of anodizing, and finally the outer part of AAO films was partly dissolved or detached after 30 min of anodizing, resulting in non-uniform surface structures of the AAO films.

A Study on Wastewater Treatment by Electrochemical Treatment with Various Electrode Interval (전극 간격에 따른 전기화학적 처리를 통한 폐수처리에 관한 연구)

  • Song, Ju Yeong
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.2
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    • pp.417-423
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    • 2019
  • A new wastewater treatment system was developed to remove nitrate nitrogen and phosphorus in synthetic wastewater through electrochemical treatment. Higher removal efficiencies of nitrate nitrogen were obtained as the current density increased. Higher nitrate removal efficiencies were obtained when the switching interval was 1 min. The total phosphorus removal rate according to the current density was found to be over 90% without being greatly affected by the change in current density and interval, and the total removal rate increased with increasing switching time (1 min interval). On the other hand, COD was not treated by electrochemical treatment, but rather increased as the electrode eluted. Also, the consumption rate of the electrode was smaller as the switching interval was shorter. Finally, removal efficiencies of 98.1% of nitrate and 90% of phosphorus were obtained through electrochemical treatment (current density $50mA/cm^2$, switching interval 1 min, flow rate 540 mL/min).

Evaluation of Non-Thermal Decontamination Processes to Have the Equivalence of Thermal Process in Raw Ground Chicken

  • Park, Eunyoung;Park, Sangeun;Hwang, Jeong Hyeon;Jung, Ah Hyun;Park, Sung Hee;Yoon, Yohan
    • Food Science of Animal Resources
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    • v.42 no.1
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    • pp.142-152
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    • 2022
  • The present study was aimed at examining the antibacterial effects of nonthermal decontamination processes, which are equivalent to thermal treatment, to ensure microbiological safety of raw ground chicken. Escherichia coli or Salmonella were inoculated into 25 g of raw ground chicken samples. The raw ground chicken samples were non-treated or treated with high hydrostatic pressure (HHP) at 500 MPa (1-7 min), light-emitting diode (LED) irradiation at 405 nm wavelength (30-120 min), and heat at 70℃, 90℃ (1-60 min), and 121℃ (1-15 min). E. coli and Salmonella cell counts were enumerated after treatments. Moreover, the color parameters of treated raw ground chicken were analyzed. HHP treatment reduced E. coli and Salmonella cell counts by more than 5 Log CFU/g and more than 6 Log CFU/g after 7 min and 1 min, respectively; these effects were equivalent to those of thermal treatment. However, LED irradiation reduced Salmonella cell counts by only 0.9 Log CFU/g after 90 min of treatment, and it did not reduce E. coli cell counts for 90 min. Compared with those of the non-treated samples, the ΔE (total color difference) values of the samples treated with HHP were high, whereas the ΔE values of the samples treated with LED irradiation were low (1.93-2.98). These results indicate that despite color change by HHP treatment, HHP treatment at 500 MPa could be used as a non-thermal decontamination process equivalent to thermal treatment.

Cytotoxicity of Impregnated Dental Gingival Retraction Cord Extracts in Immortalized Human Oral Fibroblasts and Keratinocytes in vitro

  • Myung-Jin Lee;Song-Yi Yang
    • Korean Journal of Materials Research
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    • v.33 no.4
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    • pp.130-134
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    • 2023
  • This study evaluated cell viability and cytokine release in immortalized human oral fibroblasts (hTERT-hNOFs) and keratinocytes (IHOK) exposed to a dental-impregnated gingival retraction cord. To prepare the extracts, dental gingival retraction cords impregnated with aluminum chloride hexahydrate were immersed in a cell culture medium for 24 h at 37 ℃. hTERT-hNOFs and IHOK were cultured for 24 h. The cell culture medium was removed and extracts of the dental gingival retraction cords were added. After incubation with the extract solution, cell viability was evaluated using an MTT assay. The levels of the cytokines IL-1α and IL-8 were measured in the supernatants of each cell type. The cell viability after exposure to the extract solution for 10 min exceeded 70 % in both cell types. The ET50 values for hTERT-hNOF and IHOK were 35.75 and 28.98 min, respectively. For IHOK, the IL-1α level was (5.35 ± 5.22) pg/mL at 10 min, (3.58 ± 5.38) pg/mL at 20 min, and (2.85 ± 4.28) pg/mL at 60 min of exposure (p > 0.05). The IL-8 level in IHOK was (67.16 ± 18.70) pg/mL at 10 min, (78.36 ± 7.50) pg/mL at 20 min, and (111.9 ± 26.10) pg/mL at 60 min of exposure (p > 0.05). Cytokine release was not observed from hTERT-hNOFs. Based on these results, cell viability and cytokine release were confirmed in cells exposed to the impregnated gingival retraction cord. In addition, the application of the extracts to hTERT-hNOF and IHOK during the actual contact time and determination of ET50 may be beneficial for evaluating the biocompatibility of dental-impregnated gingival retraction cords.

Development of a multi-purpose driving platform for Radish and Chinese cabbage harvester (무·배추 수확 작업을 위한 다목적 주행플랫폼 개발)

  • H. N. Lee;Y. J. Kim
    • Journal of Drive and Control
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    • v.20 no.3
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    • pp.35-41
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    • 2023
  • Radish and Chinese cabbage are the most produced and consumed vegetables in Korea. The mechanization of harvesting operations is necessary to minimize the need for manual labor. This study to develop and evaluate the performance of a multi-purpose driving platform that can apply modular Radish and Chinese cabbage harvesting devices. The multi-purpose driving platform consisted of driving, device control, engine, hydraulic, harvesting, conveying, and loading part. Radish and Chinese cabbage harvesting conducted using the multi-purpose driving platform each harvesting module. The performance of the multi-purpose driving platform was evaluated the field efficiency and loss rate. The total Radish harvesting operation time 34.3 min., including 28.8 min., of harvesting time, 1.9 min., of turning time, and 3.6 min., of replacement time of bulk bag. During Radish harvesting, the field efficiency and average loss rate of the multi-purpose driving platform were 2.0 hr/10a and 3.1 %. Chinese cabbage harvesting operation 49.3 min., including 26.6 min., of harvesting time, 4.6 min., of turning time, and 18.1 min., of replacement time of bulk bag. During Chinese cabbage harvesting, the field efficiency and average loss rate of the multi-purpose driving platform 2.1 hr/10a and 0.1 %. Performance evaluation of the multi-purpose driving platform that harvesting work was possible by installing Radish and Chinese cabbage harvest modules. Performance analysis through harvest performance evaluation in various Radish and Chinese cabbage cultivation environments is necessary.

Assessment on Gastrointestinal Transit Movement of Capsule Endoscopy in Beagle Dogs (비글견에서 캡슐 내시경의 위장관 이동분석 평가)

  • Chang, Hwa-Seok;Yang, Hee-Taek;Kim, Sang-Youn;Woo, Dong-Cheol;Park, Woo-Dae;Yong, Joon-Hwan;Choe, Bo-Young;Kim, Hwi-Yool;Choi, Chi-Bong
    • Progress in Medical Physics
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    • v.19 no.2
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    • pp.125-130
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    • 2008
  • The object of this study is to measure the transit time and passage rate of capsule endoscopy (CE) in the gastrointestinal tract in medium sized beagle dogs ($7{\sim}13\;kg$). Animals were divided into four groups: only capsule (group 1, n=10), capsule+water (group 2, n=10), mettoclopramide+capsule (group 3, n=10), metoclopramide +capsule+water (group 4, n=10). The capsule transit times through the stomach and small bowel were evaluated by radiography findings. Gastric transit time (GTT), small intestinal transit time (SITT) and complete passage rate were measured in four groups. GTT's for each group were as follows; $45{\pm}20\;min$ (group 1), $117{\pm}35\;min$ (group 2), $150{\pm}40\;min$ (group 3), and $154{\pm}65\;min$ (group 4), while SITT's were $75{\pm}20\;min$ (group 1), $195{\pm}55\;min$ (group 2), $70{\pm}15\;min$ (group 3), and $76{\pm}15\;min$ (group 4). The complete passage rates were 20% (group 1), 40% (group 2), 20% (group 3), 50% (group 4). In all groups, if CE could pass through the pylorus, it passed all small intestinal tracts within 8 hours (battery life). Administration of water helped CE to pass pylori, except in case of metoclopramide administration. These results indicate that CE could be an useful tool for examining gastrointestinal diseases in the veterinary medicine.

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Predictions of VO2max Using Metabolical Responses in Submaximal Exercise and 1,200 m Running for Male, and the Validity of These Prediction Models (성인 남성의 최대하 운동시 대사반응 및 1,200 m 달리기 기록을 이용한 최대산소섭취량 추정식 개발 및 타당도)

  • Im, J.H.;Jeon, Y.J.;Jang, H.K.;Kim, H.J.;Kim, K.H.;Lee, B.K.
    • Exercise Science
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    • v.21 no.2
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    • pp.231-242
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    • 2012
  • The purpose of this study was to develop the prediction model of VO2max using submaximal metabolic responses from the Bruce protocol, HR responses at several stages and 1,200 m running record, and to compare and analyse the validity of these prediction models. The subjects were consisted of 255 male(133 male for 1,200 m running). They were participated maximal exercise testing with Bruce protocol, and the metabolic responses were measured in the end of the first(3 minute), second stage(6 minute), and 1,200 m running record. Measurement items were VO2(㎖/kg/min), VCO2(㎖/kg/min), VE(L/min), HR(bpm) of 3 and 6 minute, time to HR 150 bpm and 170 bpm, HR difference between Bruce protocol 6 and 3 minute, 1,200 m running record. Analyzing with all variables using enter method, the multiple R of total variable model was 0.642(p<.01), SEE was 4.38 ㎖/kg/min, CV was 10.8 %, but multicolinearity was appeared. The multiple R of 3 minutes model 1 and model 2 were 0.341 and 0.461, SEE was 6.05 and 5.72 ㎖/kg/min, CV was 14.9 and 14.1%, and multicolinearity did not appeared. The multiple R of 6 minutes model 1 and model 2 were 0.350 and 0.456, SEE was 6.03 and 5.74 ㎖/kg/min, CV was 14.9 and 14.2%, and multicolinearity did not appeared. The R of HR 170 and HR 170 model were 0.151 and 0.154, SEE were 6.36~6.37 ㎖/kg/min, CV were 15.7%. The R of 1,200 m running model was 0.444, SEE was 4.82 ㎖/kg/min, CV were 11.9%. In conclusion, with considering usefulness and convenience through the validity of these prediction models, the prediction model of VO2max recommended 6 and 3 minute model, and the validity of HR model and 1,200 m running model were moderately low.