• 제목/요약/키워드: milk powder

검색결과 308건 처리시간 0.025초

공액리놀레산 생성 Lactobacillus plantarum 선발 및 이를 이용한 콩-분말 두유에서 공액리놀레산 생산 (Screening of conjugated linoleic acid (CLA) producing Lactobacillus plantarum and production of CLA on soy-powder milk by these stains)

  • 김바오로;이병원;황정은;이유영;이춘우;김병주;박지영;심은영;모하메드 아지줄 하크만;이동훈;이진환;안민주;이희율;고종민;김현태;조계만
    • 미생물학회지
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    • 제51권3호
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    • pp.231-240
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    • 2015
  • 본 연구에서는 발효식품으로부터 16종의 공액리놀레산(CLA) 생성 젖산균을 분리하였다. 이들 균주 중, S48 및 P1201 균주는 다른 젖산균들보다 더 높은 CLA를 생성하였다. 두 균주는 형태학적, 생리학적, 화학적 및 분자유전학적 특징에 따라 Lactobacillus plantarum로 동정하였다. pH 2.0 인공위액산에서 4시간 배양 후 이들 균주의 생존율은 각각 59.57% 및 6.22%을 나타내었다. 이들 균주는 $37^{\circ}C$에서 48시간 동안 상이한 자유 리놀레산 함량이 함유되어 있는 8% 탈지분유 배지에서 cis-9, trans-11 및 trans-10, cis-12 CLA 이성체가 생성되었고, 두 CLA 이성체는 48시간 배양 동안 지속적으로 증가하였다. 발효 48시간 후, 증자콩-분말 두유의 CLA 함량은 생콩 및 볶음콩-분말 두유에서 보다 높은 생성능을 보였다. 특히, 증자콩-분말 두유의 CLA 함량은 각각 S48 및 P1201 균주에서 $183.57{\mu}g/ml$$198.72{\mu}g/ml$ 생성되었다.

Antioxidant, antibacterial, and antiinflammatory effects of yoghurt made with vitamin tree (Hippophae rhamnoides L.) fruit powder

  • Byung Bae Park;Gereltuya Renchinkhand;Woo Jin Ki;Jong Woo Choi;Myoung Soo Nam
    • 농업과학연구
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    • 제49권4호
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    • pp.905-917
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    • 2022
  • It is well known that the fruit of the vitamin tree (Hippophae rhamnoides L.) provides excellent anti-diabetic, antibacterial, immune regulation, anti-inflammatory, and anti-aging effects. In some countries including Europe the fruit has been added to certain foods to develop functional foods. The present research was carried out to elucidate the biological function of vitamin tree fruit powder added to fermented milk. It was found that there was an antioxidant effect of yoghurt made with vitamin tree fruit powder, and this effect was greater with increased incubation time and amount of vitamin powder, as shown by 1,1-diphenyl2-picrylhydrazyl (DPPH) and 2,2-anziobis (3-ehtylbenzothiazoline-6-sulfonic aicd) (ABTS) radical scavenging activities. The antibacterial effect of yoghurt containing vitamin tree fruit powder was shown to be effective against four pathogenic microorganisms, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonela Typhimurium. In particular, yoghurt supplemented with 5% of vitamin tree fruit powder showed the best antibacterial effect. The yogurt containing the vitamin tree fruit powder significantly inhibited the expression of the anti-inflammatory cytokines interleukin (IL)-6 (yogurt [Y] + Hippophae rhamnoides L. powder [HP] and yogurt containing 5% Hippophae rhamnoides L. powder [HPY]) and IL-1β (HP, Y + HP and HPY) in a concentration-dependent manner among tumor necrosis factor (TNF)-α, IL-6, IL-1, and induced nitric oxide synthase (iNOS). Our results clearly demonstrated that vitamin tree fruit powder could be a good functional ingredient for improving health through yoghurt manufactured with vitamin tree.

영아의 수유 및 보충식에 대한 조사연구 (A Survey Of Infant Feeding Practices In Seoul, 1991.)

  • 김효진;박영숙
    • 대한간호학회지
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    • 제23권3호
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    • pp.377-398
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    • 1993
  • A survey of infant practices was conducted to provide information on which to base planned nursing interventions. The subjects were a convenience sample of 168 mothers visiting out - patients departments of five general hospitals and one public health center in Seoul for immunizations or treatment for common colds for their infants from two to 12 months of age. Data collection was carried out from July 8th to September 30th, 1991 using a questionnaire of 84 questions, 31 on the type of feeding, 22 on supplementary feeding and 21 on demographic infer mation. The results are summarized as follows : 1) The type of feeding was primarily artificial feed-ing (63.1%), followed by mixed feeding (22%) and breast feeding (14.9%) of the 59 mothers expecting during pregnancy to breast feed, 54. 2% changed artificial feeding and 30.5% went on to breast feed as they had expected. For mothers expecting to continue breast feeding over seven months. only one infant was being breast fed for over seven months. 2) For the 106 mothers using artificial feeding, 70. 8% hed attempted breast feeding, 64% of them for less than a month. Breast milk had been suppressed by for medication (38.7%) : 34.9% had used no specia] means. 3) The major reasons for replacing breast feeding with artificial feeding were the infants' demand for more milk (47.2% ) and insufficient supply of breast milk (49.3%). 4) Most mixed feeding was started at the age of one to three months (59.5%). Only 34.4% gave an artificial feeding after breast feeding : most (46%) alternated breast feeding with artificial feeding. On the whole, the motive for mixed feeding was the lack of breast milk (70.3%). 5) Many mothers (81.8%) were adding vitamin or mineral supplements to artificial milk and 51.5% were adding something to faciliate digestion. As for the method of sterilizing milk bottles and nipples, 56% had sterilized them together in boiling water from the beginning : 27% were just washing the bottles after boiling only once initially when measuring artificial milk powder, 31. 5% of the mothers over filled the measuring spoon rather than to the level. 6) The mother's occupation was related to her way of feeding. Mothers at home full time did more breast feeding than mothers employed outside the home. (x²=5.72, p=〈0.05). 7) Most mothers began supplementary food, from three to four months (48.8%) : 11.2% began later than seven months. Supplementary food was given between milk feedings by 67.2% of the mothers : 19.2% gave it before a milk feeding. Some mothers(26.4%) made their own supplementary food : 19.2% used ready - made supplementary food products for convenience. Recommendations for nursing interventions included : 1. Prenatal education about the advantages of breast feeding and breast care, and home visits after delivery for counselling related to breast feeding. Correct preparation of artificial feeding methods need to be taught in both pre & postnatal periods. In addition, specific education about supplementary feeding needed. 2. Further research is indicated about the Perceived lack of supply of breast milk and about the effectiveness of nursing interventions to Promote breast feeding.

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동결건조에 따른 살충성 세균 Photorhabdus temperata M1021의 안정성과 살충성 평가 (Insecticidal Activity and Stability by Freeze-drying of Entomopathogenic Bacteria, Photorhabdus temperata M1021)

  • 박건석;장은경;김민성;신재호
    • Journal of Applied Biological Chemistry
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    • 제55권2호
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    • pp.123-127
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    • 2012
  • 오늘날 환경친화적인 생물농약을 개발하기 위한 미생물로는 Bacillus thuringiensis 이외에 Photorhabdus, Xenorhabdus 및 Serratia와 같은 곤충병원성 미생물과 Pseudomonas와 같은 식물유용 미생물에 대한 연구가 활발히 진행되고 있다. 본 연구에서는 곤충병원성 미생물인 Photorhabdus temperata M1021 균주를 동결건조법을 이용하여 제제화 하였으며, 동결건조 시 세포보호를 위하여 skim milk, starch, sodium alginate, glucose와 sodium glutamate를 농도별로 첨가하여 동결 건조 후의 세포 생존율을 확인하였다. 그 결과 7% (w/v)의 skim milk가 첨가된 시료에서 가장 높은 63%의 생존율을 나타내었다. 제제화된 동결 건조균을 공기와 접촉시키면서 저온에서 생존율을 측정한 결과 4주 후에도 75% 이상의 생존율을 나타내었다. 또한 제제를 이용한 살충력 시험에서 꿀벌 부채명나방 유충에 대한 주사독성은 $2.0{\times}10^1$ cells/lavar 이상을 주사할 경우 4일 이내에 전체유충이 사멸하는 것으로 나타났으며, $2.0{\times}10^0$ cells/larva의 아주 낮은 농도에서도 50% 이상의 유충사멸 효과를 확인하였다. 본 연구에서 사용된 P. temperata M1021 균주의 동결건조 분말의 뛰어난 살충효과는 보다 현실적인 생물학적 제제로의 개발가능성을 제시하고 있다.

국민식생활(國民食生活) 향상(向上)을 위(爲)한 곡류제품(穀類製品)의 경제적( 經濟的) 영향강화(營養强化)에 관(關)한 연구(硏究) (A Study on the Economical Nutrition Supplement of Cereal Food for Improvement in our National Eating Habits)

  • 주진순;유종열;김숙희;이기열;한인규
    • Journal of Nutrition and Health
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    • 제6권1호
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    • pp.1-9
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    • 1973
  • I. Subject of the Study: Studies on the economical nutrition supplement of cereal foods for the improvement in our notional eating habits. II. Purpose and Importance of the Study: 1. Our nation is confronted with the situation that the rice, a principal food, short of some essential amino acids, lysine and threonine, leads to imbalanced meals insufficient in the nutrient of protein, to bring many difficulties in the elevation of our national physique. 2. The shortage of even the rice imperfect in the nutrient of protein makes the import of lots of foreign rice inevitable. It is considered that the protein supplement and decrease in the consumption amount, of rice, is a serious key to the solution of our food difficulty, and then a way of the proetin supplement of rice through the addition of essential amino acid is to be rarely applied in the view of the our present finance and situation. 3. In the present experiment, therefore, it aims to the suggestion of an aspect of the improvement in our national eating habits guiding in the nutrition elevation which our nation can afford economically through the development of first, a way of the protein supplement by the mixture of cereals producted plentifully in our country, and second, a way of the decrease in the consumption amount and the improvement in the nutrition of rice through the substitution of the other cereals for rice. III. Contents of Scope of the Study: 1. Objects of the study: Objects of the study are the following three items; a) The nutrition supplement of rice through the mixture of cereals. Our nation makes mainly rice as a principal food, but practically many kinds of cereal are produced in our country. They contain different levels and qualities of each nutrient and they are different from one another in the kinds of essential amino acid consisting protein. For that reason, the mutual complement efficeincy of insufficient nutrients is observed through the mixture of cereals. b) The nutrition supplement of rice through the addition of superior protein sources to rice, a principal food. The development of rice as superior foods in the sense of nutrition is conducted through the risement in protein quality by the addition of protein sources in good quality, for example, fish flour (anchovy flour), egg powder, milk powder, and so on, and through the supplement of vitamins and minerals. c) The decrease in the consumption amount of rice through the substitution of the other cereals for rice, as a principal food. The compensation for the short amount of rice is made by the reduction in the consumption of rice through the discovery of a way of substitution of the other cereals for rice, as a principal food and of the settlement of problems in nutrition and finance subsequent to this. 2. Contents of the study: a) An ideal mixture-ratio of cereals is established for rats by feeding mixed foods(rice-barely or rice-wheat) containing 5%, 15%, 25%, 35% and 45% level of either barely or wheat. b) The nutritive value is determined in the whole subsititution of other foods for rice, and then, a way of the complement of over and under nutrients is devised. c) The ideal combination is investigated for rats through feeding mixed foods of main food, rice and supplement foods of protein sources, soy bean, fish flour, egg powder and milk Powder. d) According to results from the above three experiments, the concise functional test for men and the examination of economical property are made. 3. Scope of the study: a) The observation of the effect of each diet on the growth rate for rats. The growth rate of rats was observed for 15 groups of mixed foods of a main food, rice, and wheat flour, barley powder or soy bean powder, respectively, and 12 groups of wheat flour diets supplemented with $1{\sim}3%$ milk powder, and rice or wheat flour diets supplemented with 5% of milk powder, egg powder, fish flour or soy bean powder, respectively. b) The determination of food consumption. The food consumption was determined at weekly intervals for 27 kinds of diet described in a) item. c) The determination of food efficiency rate. The food efficiency rate for each diet was determined by calculation from the gained body weight and the food consumption amount at the same intervals described in b) item. d) The determination of protein efficiency rate. The protein efficiency rate for each diet was determined by calculation form gained body weight and the protein amount of the food consumption amount at the same intervals described c) item. e) The determination of the body component. The hematocrite and hemoglobin levels in the blood, total nitrogen in the serum, blood sugar, and lipids and glycogen in the liver were determined. f) The observation of nitrogen balance. As a means of the observation of nitrogen balance, the total nitrogen in the urine was determined. g) The analysis of economical property. The economical property was analyzed as the gained body weight to the amount equivalent to one won through the conversion of the food consumption amount into money. h) The functional test for men. The concise functional test for men was made in order to establish if the best diet for experimental animals can be applied to men. IV. Results of the Study: The national food product plan, nationwide nutritive enlightment and the improvement activities in our country eating habits, especially, mixed and powder food problems are to be significantly referred, and the following results must be applied. a) In the mixed foods of cereals, the mixed food of the rice-barley containing $5{\sim}15%$ level of barley is best in terms of nutrition. b) The addition of superior protein sources, egg, Bilk, soy bean, or fish, respectively to either rice or wheat flour makes a great risement in the nutritive value. c) The animal protein is more effective in the elevation of nutritive value of cereals. d) Rice takes the most nutritive operation and has the highest preference, among rice, wheat flour and barley. e) Wheat flour is more economical than rice in evaluation of the gained body weight to the regular money, and the addition of fish or soy bean is more economical than that of any other supplement food. But the above results are true of the range of nutrition and economical property. f) The study on the nutrition composition and barley will lead to the improvement in our national eating habits as mixed food of the rice-barley containing $5{\sim}15%$ level of barley is more nutritive. g) This study on the nutrition only for the growing animal can not be considered as a perfect and entire evaluation. Consequently, the perfect data for our national nutrition can be obtained from the experiment similar to this for the much longer period examining, in details, the growth rate, change of physical strength, mental and bodily change, average life span, and resistance ability to infectious diseases.

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머랭을 이용하여 제조한 녹차증편의 품질특성 (Quality Characteristics of Green Tea Jeungpyun Made with Meringue)

  • 박재희
    • 한국식품조리과학회지
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    • 제33권1호
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    • pp.37-44
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    • 2017
  • Purpose: The purpose of this study was to simplify the traditional manufacturing method of Jeungpyun by developing a new process for Jeungpyun preparation using meringue and to also investigate the quality characteristics of Jeungpyun made with green tea powder. Methods: The Jeungpyun was manufactured as 4 sample preparations (Jeungpyun: J; Jeungpyun+0.5% green tea: J+0.5% GT; Jeungpyun+1% green tea: J+1% GT; and Jeungpyun+1.5% green tea: J+1.5% GT) through mixing rice flour, meringue, green tea powder (0-1.5%), dry yeast, water, and milk, steaming for 4 min at $180^{\circ}C$ and 3 min at $220^{\circ}C$, and then standing for 1 hr at room temperature. The quality characteristics of Jeungpyun were evaluated based on physicochemical properties, total mold, texture analysis, and sensory evaluation. Results: The pH of Jeungpyun batter was significantly lower in J+1.0% GT and J+1.5% GT than J. The specific gravity, weight, volume, and specific volume of J+0.5% GT and J+1% GT was not significantly different, as compared to J. In color, L value decreased and a and b value increased based on the increase of green tea powder. The antifungal activity was the highest in J+1.5% GT, followed by J+1.0% GT, J+0.5% GT, and J, in order. Samples with added green tea powder showed decreased hardness, as compared with J; its effect was significant in J+1.0% GT and J+1.5% GT after storage for 1 day. In sensory evaluation, the color of Jeungpyun was darkened by the addition of green tea powder. The egg smell and hardness significantly decreased by addition of green tea powder. Based on quantitative description analyses, overall acceptability was the highest in J+1% GT. Conclusion: Therefore, 1% green tea powder was the optimal amount for preparing Jeungpyun with meringue. For simplification of the traditional manufacturing method, Jeungpyun could be produced with meringue and green tea powder, which has potent physiological activities.

초석잠 분말을 첨가한 두부의 항산화 활성 및 품질 특성 (Antioxidant Activities and Quality Characteristics of Tofu Supplemented with Chinese Artichoke Powder)

  • 이지은;진소연;한영실
    • 한국식품영양학회지
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    • 제27권1호
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    • pp.10-21
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    • 2014
  • This study is conducted to investigate the effects of Chinese artichoke powder on the antioxidant activities and the quality characteristics of tofu. Tofus are being prepared with different amounts of Chinese artichoke powder in ratios of 0, 0.2, 0.4, 0.6 and 0.8% according to the soy milk quantity. According to the increasing contents of Chinese artichoke powder, the yield and turbidity of tofus are significantly increased (p<0.001) whereas the pH levels of tofus are significantly decreased (p<0.001). In terms of color, the L, a and b values are significantly decreased with increasing Chinese artichoke powder concentrations (p<0.001). From the texture profile analysis of the tofus, the chewiness, gumminess and cohesiveness of tofus are significantly decreased (p>0.01) according to the levels of added Chinese artichoke powder content. The contents of isoflavones such as daidzin and genistin are significantly increased with increasing concentrations of Chinese artichoke powder (p<0.001). The microstructure of tofus is being examined by SEM (Scanning Electron Microscope). In the sensory test, the sample which contains 0.4% of Chinese artichoke powder is ranked significantly higher (p>0.001) than the other groups according to several sensory parameters such as appearance, color, flavor, taste, texture and the overall preferences.

유도결합 플라스마-질량분석법에 의한 혈청, 생우유 및 사료첨가제중 요오드의 분석에 관한 연구 (A Study on Determination of Iodine in Serum, Fresh Milk, and Feed Additive by Inductively Coupled Plasma-Mass Spectrometry)

  • 이원;박경수;김선태;김영만
    • 분석과학
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    • 제12권6호
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    • pp.528-533
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    • 1999
  • 유도결합 플라스마-질랑분석법을 이용하여 혈청, 생우유 및 사료첨가제 중 요오드를 정량하였다. 시료를 매트릭스 변형제인 $NH_4OH$(0.5% v/v)+$CH_3OH$(5% v/v) 혼합용액으로 25배 묽힌 다음, m/z=127에서 이온세기를 측정하였다. 이 방법에 대한 요오드의 검정곡선은 $0-100{\mu}g/L$ 범위에서 상관계수($R^2$)가 0.99인 직선관계를 나타내었으며, 검출한계는 $0.084{\mu}g/L$이었다. 최적 분석 및 기기 조건에서 분유 표준시료를 분석한 결과 상대오차는 2.30-4.73% 범위이었다. 또한 혈청, 생우유, 동물의 사료 첨가제 등과 같은 실제시료를 분석한 결과 요오드의 함유량은 각각 $12.4-40.2{\mu}g/L$, < 0.01-3.11 mg/L,< $10^{-7}-2.60g/kg$ 범위이었다.

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고령자를 위한 영양강화 유제품 개발 II. 고령자 영양강화 적용 기술 현황 (Application of Dairy Food Processing Technology Supplemented with Enriched-nutrients for the Elderly: II. The Applicable Technology of Carefoods for the Elderly)

  • 김범근;장혜원;최가희;문용일;오세종;박동준
    • Journal of Dairy Science and Biotechnology
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    • 제37권4호
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    • pp.213-222
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    • 2019
  • Milk and dairy products are the high value foods for the elderly population. In particular, fermented milk is the best source of calcium for people in the specific age group of over 79 years. It provides a good source of protein. Regular exercise and active lifestyle are recommended to slow down the muscle loss. However, exercising without proper nutrient intake is simply not sufficient at this age. Milk and dairy products provide the iron and protein content required for effective exercise-assisted growth. Milk nutrients have the advantage of being produced in various food forms, such as liquid, semi-solid, and powder types. Fat-soluble vitamins such as retinol and vitamin K can be encapsulated using various technologies for milk and dairy products. Using the encapsulation method, spray drying and fluidized-bed coating have been used for adding the micro-nutrients to the food. Microencapsulation technology is being applied in case of the fermented dairy products too. In particular, various wall materials are being developed to enhance the viability of probiotics. In the near future, advanced high-efficiency technologies that can effectively nourish the dairy products with nutrients will be developed to produce targeted high-nutrition value food for the elderly.

Characterization of Lactobacillus acidophilus Isolated from Piglets and Chicken

  • Ahn, Y.T.;Lim, K.L.;Ryu, J.C.;Kang, D.K.;Ham, J.S.;Jang, Y.H.;Kim, H.U.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권12호
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    • pp.1790-1797
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    • 2002
  • Lactic acid bacteria were isolated from piglets and chicken and characterized. Lactic acid bacteria showing resistance to low pH and bile, adhesion to intestinal epithelium cells, and the inhibition of Escherichia coli and Salmonella spp. were identified as Lactobacillus acidophilus. L. acidophilus PF01 survived for 2 h in MRS broth adjusted to pH 2. L. acidophilus CF07 was less resistant than L. acidophilus PF01 to pH 2, but survived at pH 2.5 for 2 h. Both of isolates were able to grow in MRS broth containing 0.3% (w/v) bile, with L. acidophilus CF07 being more tolerant to bile than L. acidophilus PF01. L. acidophilus PF01 and CF07 adhered specifically to the duodenal and jejunal epithelium cells of piglet, and the cecal and duodenal epithelium cells of chicken, respectively. Both of isolates did not adhere to the epithelium cells of the various animal intestines from which they were isolated. When L. acidophilus was cultured with E. coli and Salmonella spp. in MRS broth, MRS broth containing 2% skim milk powder or modified tryptic soy broth at $37^{\circ}C$, L. acidophilus PF01 and CF07 inhibited the growths of E. coli K88 and K99, and S. enteritidis and S. typhimurium, respectively. Both of isolates were found to possess the essential characteristics of probiotic lactic acid bacteria for piglet and chicken.