• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.899-902
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• 2005

Peptide assimilation by Helicobacter pylori was investigated using L-phenylalanyl-3-thia-phenylalanine (PSP) as a detector peptide; the release of thiophenol upon enzymatic hydrolysis of PSP was spectrophotometrically detected with the aid of 5,5'-dithiobis[2-nitrobenzoic acid] (DTNB). By adding PSP to whole-cell suspension, thiophenol was produced progressively, resembling that found in Esherichia coli or Staphylococcus aureus. Interestingly, the rate of thiophenol production by H pylori in particular was markedly reduced when cells were pretreated with trypsin, indicating surface exhibition of peptidase. According to the competitive spectrophotometry using alanyl-peptides, H pylori did not appear to assimilate PSP through the peptide transport system. No discernible PSP assimilation could be ascertained in H pylori cells, unless provided with some additives necessary for peptidase activity, such as $Ni^{2+}\;or\;Mg^{2+}$ and an appropriate concentration of potassium or ammonium salts. These observations strongly suggest that, regardless of a presumptive peptide transport system, peptide assimilation of H. plori appears to be highly dependent upon milieu conditions, due to unique peptidase exhibition on the cell surface.

• Journal of Microbiology and Biotechnology
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• 제20권6호
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• pp.978-984
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• 2010

This paper examines the probiotic bacterium Lactobacillus rhamnosus GG, and how it reacts to the presence of mucin in its extracellular milieu. Parameters studied included cell clustering, adhesion to mucin, extracellular protein production, and formation of final metabolites. L. rhamnosus GG was found to grow efficiently in the presence of glucose, N-acetylglucosamine, or mucin (partially purified or purified) as sole carbon sources. However, it was unable to grow using other mucin constituents, such as fucose or glucuronic acid. Mucin induced noticeable changes in all the parameters studied when compared with growth using glucose, including in the formation of cell clusters, which were easily disorganized with trypsin. Mucin increased adhesion of the bacterium, and modulated the production of extracellular proteins. SDS-PAGE revealed that mucin was not degraded during L. rhamnosus GG growth, suggesting that this bacterium is able to partially use the glucidic moiety of glycoprotein. This study goes some way towards developing an understanding of the metabolic and physiological changes that L. rhamnosus GG undergoes within the human gastrointestinal tract.

• The Korean Journal of Physiology and Pharmacology
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• 제2권1호
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• pp.41-48
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• 1998

The present study was undertaken to characterize homocysteic acid (HCA)-and cysteic acid (CA)-mediated formation of inositol phosphates (InsP) in primary culture of rat cerebellar granule cells. HCA and CA stimulated InsP formation in a dose-dependent manner, which was prevented by the N-methyl-D-aspartate (NMDA) receptor antagonist D,L-2-amino-5-phosphopentanoic acid (APV). CA-, but not HCA-, mediated InsP formation was in part prevented by the metabotropic glutamate receptor antagonist ?${\alpha}$-methyl-4-carboxyphenylglycine ($({\pm})$-MCPG). Both HCA- and CA-mediated increases in intracellular calcium concentration were completely blocked by APV, but were not altered by $({\pm})$-MCPG. CA-mediated InsP formation was in part prevented by removal of endogenous glutamate. In contrast, the glutamate transport blocker L-aspartic acid-${\beta}$-hydroxamate synergistically increased CA responses. These data indicate that in cerebellar granule cells HCA mediates InsP formation wholly by activating NMDA receptor. In contrast, CA stimulates InsP formation by activating both NMDA receptor and metabotropic glutamate receptor, and in part by releasing endogenous glutamate into extracellular milieu.

• 대한수학교육학회지:수학교육학연구
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• 제9권2호
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• pp.473-491
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• 1999

The learning of mathematics happens in some situations. It is natural that students should learn mathematics in more appropriate situations. But, so far It has been hardly studied about concrete situation and milieu where math can be successfully taught. In today's math education, the situation of education as a external circumstance become realized more and more importantly with influence of open education. But they don't embody situation as an internal circumstance where the intrinsic concept of mathematics can be obtained. We started this thesis from this tried to answer it on the basis of Brousseau's question, have theory about the didactical situations. One of the purpose of this study is to understand the theory of didactical situations, which focuses on how we can elaborate situations which really make a mathematical notion function. In this study, It is attempted clarify some concepts of the theory of didactical situations. The other is to discuss about what the theory of didactical situations suggests us in math zeducation. The method of math teaching and learning and the teacher's role were discussed in the viewpoint of Brousseau's theory. Finally, We elaborated and presented some didactical situations which make the notion of the area of rectangle.

• 대한수학교육학회지:수학교육학연구
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• 제10권2호
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• pp.183-197
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• 2000

The purpose of this paper is to present the history of the research in mathematics education, its characteristic and some theories as its results in France. The french research in mathematics education really began with the inauguration of IREM in the institutional aspect, referring to Bachelard in the epistemological aspect and to Piaget in the psychological aspect. It aimed at appreciating the mathematics education as a independent science and focused on the theoretical research through its own object(didactic system) and its own method(didactic engineering). Therefore, it can be characterized by the dense and elaborate theoretical arguments. Consequently, it is known that four major theories in french mathematics education were developed: the theory of didactic situations by trousseau, the theory of didactic transposition by Chevallard, the theory of conceptual fields by Vergnaud, the theory of tool-object dialectic by Douady. Among them, this paper is focused on the situation of institutionalization and the structurization of milieu in the theory of Brousseau and the motive of didactic transposition and the didactic time in the theory of Chevallard. In that the french research in mathematics education has been founded on its own theoretical models, it may contribute to us who envy the basic theories of mathematics education.

• Journal of Korean Neurosurgical Society
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• 제45권3호
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• pp.151-156
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• 2009

Objective : It is well known that changes in cerebral hemodynamics occur after traumatic brain injury (TBI). Osmo-regulation in the brain is important for maintaining a constant milieu in the central nervous system. Nevertheless, to our knowledge, early osmolarity changes after minor head injury have not been studied until now. Methods : In this study, serum osmolarity was measured in 99 patients with minor head trauma. As a control group, blood samples were drawn from 99 patients who had a minor trauma in an extremity. Serum osmolarity was estimated using a fully automatic biochemical autoanalyzer within the first 3 hours after the trauma. Results : The mean serum osmolarity levels were $286.08{\pm}10.1\;mOsm/L$ in the study group and $290.94{\pm}5.65\;mOsm/L$ in the control group (p<0.001). However, after age adjustment between the study and control groups, this statistical significance was found to be valid only for patients over 30 years of age. Conclusion : It was noted that serum osmolarity levels decrease in the first 3 hours following minor head trauma in patients over 30 years of age. Further studies into this area could provide guidance for the management/treatment of elderly patients.

• 혜화의학회지
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• 제8권2호
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• pp.341-359
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• 2000

The following result are obtained through that comparative study on the psychotherapy of oriental and occidental medicine. 1. The shamanism(祝由) and active emotion therapy(移精變氣療法) are a primitive form of psychotherapy. It is an opinion that between active emotion therapy(移精變氣療法) and psychoanalytic therapy or supportive psychotherapy are similar. Also, the basic cognition pattern of shamanism(祝由) is projection. 2. Taoism is inseparably related to the psychotherapy of korean oriental medicine, the major thought has become the ideal base of oriental psychotherapy. 3. According to study of clinical document in relation to oriental psychotherapy, psychotherapy had been widely used by medical cure. 4. The five emotion restraint therapy is a high level psychotherapy of oriental medicine. Oriental psychotherapy that disease caused by surprising must be cure by surprising is like to systematic desensitization in western, and that pseudopsychosis must be cure by patient rely on doctor is like to suggestive therapy in western. 5. There are psychotherapy in western that Supportive psychotherapy, Short-term dynamic psychotherapy, Psychoanalytic therapy, Group psychotherapy, Family therapy, Morita therapy, Hypnotherapy, Autogenic training, Behavioral therapy, Milieu therapy, Occupational therapy, Psychodrama, the others. I think that they have wide application to the psychotherapy of korean oriental medicine.

• KIEAE Journal
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• 제8권2호
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• pp.47-52
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• 2008

A nations' cultural heritage embodies its intellectual and spiritual contributions to its civilization of mankind. Cultural properties, whether tangible or intangible, represent both the essence and the basis of national cultures. Both the Philippines and South Korea's cultural heritages have survived various unfortunate chapters of their long histories. This study's purpose is to explore and evaluate the phenomenon of the architecture of Hahoe village in Andong city, Korea and Vigan city of Ilocos Sur, Philippines and to understand and cherish the cultural heritage of both countries. The historic city of Vigan has a unique architecture that blend Ilocano, Filipino, Chinese and Spanish styles in a tropical Asian setting, with a typical Spanish colonial urban layout as specified by the Ley Delas Indias. Hahoe village also represents a masterpiece of human creation and exhibit an important interchange of human values in architecture, monumental arts, town planning and landscape design. Though diverse in milieu, Hahoe village and Vigan city share a common phenomenon which is architecture that is called cultural heritage.

• Fibers and Polymers
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• 제2권2호
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• pp.64-70
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• 2001

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

• Advances in Traditional Medicine
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• 제9권4호
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• pp.339-349
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• 2009

An attempt has been made to assess whether the dose dependent effect of benzene extract of Ocimum sanctum leaves on the morphological changes in the cauda epididymal spermatozoa and sperm parameters in male albino rats. Scanning Electron Microscope observations illustrate the disturbance in plasma membrane as well as acrosomal membrane. Most of the sperms appear morphologically abnormal in the mid region of the tail; there is formation of balloon like cytoplasmic droplet. Sperm parametric study exhibits decrease in the total sperm count, sperm motility, forward velocity and increase in the percentage of abnormal sperms in dose dependent manner on treatment benzene extract of Ocimum sanctum leaves. The results suggest that the effects may have resulted from a general disturbance in the proteins and alteration in cauda epididymal milieu probably due to androgen deficiency consequent upon antiandrogenic property of Ocimum sanctum leaves.