• Molecules and Cells
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• 제25권3호
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• pp.452-456
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• 2008

The interactions between monocytes and extracellular matrix proteins have been implicated in atherosclerosis pathophysiology. In the present study we evaluated monocyte attachment and migration through oxidized and non-oxidized collagen IV. Monocyte attachment was tested on microwells coated with either native or oxidized collagen IV. Monocyte migration through collagen IV was examined on transwells. Monocytes derived from patients with diabetes mellitus showed an increased ability to attach and migrate through collagen IV as compared to those derived from healthy volunteers. Moreover, control monocytes attached to oxidized collagen at a higher degree, while they migrated through oxidized collagen at a lower degree, as compared to the native protein. Our results also showed the involvement of the alpha2 integrin subunit in the above phenomena suggesting a modified interaction between monocytes and collagen IV in diabetes mellitus.

• 한국신뢰성학회:학술대회논문집
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• 한국신뢰성학회 2005년도 학술발표대회 논문집
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• pp.385-392
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• 2005

The restriction of the use of hazardous substances in electrical and electronic equipment legislation mandates the substitution of lead and other hazardous substances in electronics products by July 2006. Due to this legislative pressure, the electronics industry is moving to adoption of lead free solders. In this paper, we investigated a flux to restrict generating electrochemical migration in lead-free solder. The lead-free solders used in this study were Sn-0.7Cu-0.01P and Sn-3.0Ag-0.5Cu. To measure the resistance of electrochemical migration, the dew-cycle test and water drop test were adopted. As the result, now flux having high durable of electrochemical migration was developed.

• 한국축산식품학회지
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• 제35권4호
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• pp.507-514
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• 2015

Deer velvet antler (DVA) is one of the most popular medicines in China. Numerous studies have demonstrated that velvet antler possess biological effects. However, data regarding its anti-migration activity on prostate cancer is scarce. In this study, we investigated the inhibitory effect of top DVA (T-DVA) on the expression of prostate-specific antigen (PSA) and migration-related genes in the human prostate cancer cell, LNCaP. The T-DVA down-regulated the expression of PSA. In addition, the Radius^TM assay revealed that T-DVA inhibited the migration behavior of prostate cancer cells. Furthermore, the expression of matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF) was also decreased with T-DVA. On the contrary, T-DVA increased the tissue inhibition of metallo-proteinase (TIMP)-1 and (TIMP)-2. Taken together, our findings indicate that the T-DVA possesses anti-migration activity on prostate cancer cells. This is the first study of DVA to report the anti-migration activity on prostate cancer.

• 한국지역지리학회지
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• 제16권2호
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• pp.123-136
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• 2010

부산 대도시권 인구이동의 특성을 밝히기 위해, 본 연구에서는 이동 가구의 특성과 부산 대도시권 인구이동의 선택성을 분석하였다. 주요 연구 결과는 다음과 같다. 첫째, 부산에서 유출되는 가구의 가구주는 25~34세 연령대의비율이 가장 높다. 비이동 가구에 비해 이동 가구는 가구주의 학력 수준이 높고, 1인 가구 비율이 높으며, 자가 거주비율이 낮다. 특히 부산에서 수도권으로 전출하는 가구에서는 이러한 경향이 더욱 강하다. 둘째, 연령과 학력에 따른 인구이동의 선택성이 뚜렷하게 나타난다. 부산은 모든 연령대에서 인구가 유출되는 가운데, 10대와 20대의 전출 초과규모가 크다. 비이동자에 비해 이동자는 고학력자 비율이 높으며, 특히 부산에서 수도권으로 전출하는 인구의 고학력자 비율이 높다. 부산의 4개 사례 고등학교를 대상으로 2008년 진학 현황을 살펴본 결과, 성적이 우수한 학생들은 대부분 서울로 진학하였다. 부산은 인구가 과도하게 유출되고 있는 양적 문제와 함께 두뇌유출이라는 질적 문제를 동시에 겪고 있다.

• 한국임상수의학회지
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• 제36권4호
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• pp.200-206
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• 2019

This study was performed to assess the anti-inflammatory and cartilage regenerative effects of platelet-rich plasma (PRP) on canine chondrocytes. Proliferation and migration assays under both normal and lipopolysaccharide (LPS)-induced inflammatory conditions were performed with various concentrations of PRP (1% to 10%). The expression levels of genes related to osteoarthritis were evaluated in the following groups: PRP group, LPS group and LPS + PRP group. mRNA expression levels were detected using real-time polymerase chain reaction (RT-PCR). Proliferation assays showed significantly enhanced proliferation in all PRP-treated groups compared with the no serum group. Compared with 10% fetal bovine serum (FBS), PRP concentrations above 3% in the normal condition and 1% to 7% PRP in the LPS-induced inflammatory condition were found to significantly promote chondrocyte proliferation. In the normal condition, all PRP-treated groups showed significantly increased cell migration compared with the no serum group. Chondrocyte migration was decreased with LPS-induced inflammation, but PRP treatment resulted in significantly enhanced migration compared with the other groups in this condition. According to RT-PCR, the LPS + PRP group showed significantly higher levels of COL1A1, IL-6, aggrecan and lower levels of $TNF-{\alpha}$, MMP-1, MMP-3 mRNA expression compared to the LPS group. The results of this study suggest that PRP application can enhance the proliferation and migration of canine chondrocytes and improve canine articular cartilage regeneration.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.792-799
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• 2022

As a vital problem in reproductive health, recurrent spontaneous abortion (RSA) affects about 1% of women. We performed this study with an aim to explore the molecular mechanism of interleukin-23 (IL-23) and find optimal or effective methods to improve RSA. First, ELISA was applied to evaluate the expressions of IL-23 and its receptor in HTR-8/SVneo cells after IL-23 treatment. CCK-8, TUNEL, wound healing and transwell assays were employed to assess the proliferation, apoptosis, migration and invasion of HTR-8/SVneo cells, respectively. Additionally, the expressions of apoptosis-, migration-, epithelial-mesenchymal transition- (EMT-) and p38 MAPK signaling pathway-related proteins were measured by western blotting. To further investigate the relationship between IL-23 and p38 MAPK signaling pathway, HTR-8/SVneo cells were treated for 1 h with p38 MAPK inhibitor SB239063, followed by a series of cellular experiments on proliferation, apoptosis, migration and invasion, as aforementioned. The results showed that IL-23 and its receptors were greatly elevated in IL-23-treated HTR-8/SVneo cells. Additionally, IL-23 demonstrated suppressive effects on the proliferation, apoptosis, migration, invasion and EMT of IL-23-treated HTR-8/SVneo cells. More importantly, the molecular mechanism of IL-23 was revealed in this study; that is to say, IL-23 inhibited the proliferation, apoptosis, migration, invasion and EMT of IL-23-treated HTR-8/SVneo cells via activating p38 MAPK signaling pathway. In conclusion, IL-23 inhibits trophoblast proliferation, migration, and EMT via activating p38 MAPK signaling pathway, suggesting that IL-23 might be a novel target for the improvement of RSA.

• 한국정보통신학회논문지
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• 제17권2호
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• pp.488-494
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• 2013

센서네트워크의 능동성과 자율성을 위한 이동에이전트의 효율적 이주 방법과 일관된 네이밍 서비스는 센서네트워크 미들웨어의 주요 요소이다. 따라서 본 논문에서는 센서네트워크 구성 성분들에 대한 다양한 정보를 새로운 메타테이블에 저장하여 RMI 기반의 네이밍 기법을 통하여 능동규칙 이동에이전트의 역 방향 이주와 탑재한 능동규칙 실행을 실험한다. 본 연구는 기존 정 방향 이주[12]의 확장을 기반으로 이루어지며, 실험 결과는 센서네트워크 환경에서의 이동에이전트 미들웨어의 유효성과 효율적인 센서네트워크 응용환경 구축 가능성을 제시하며, 이는 센서네트워크 응용 개발에 있어서 동적인 환경 변화에 대한 적응성을 향상시킬 수 있다.

• Child Health Nursing Research
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• 제26권4호
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• pp.463-469
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• 2020

Reduction of the under-5 mortality rate is a target of the Sustainable Development Goals. Therefore, this study aimed to estimate under-5 child mortality rates in 52 low-migration countries using population data. The study utilized population data from the US Census Bureau from 1990 to 2015. The method involved first estimating mortality rates for countries with negligible net migration and then applying these rates to countries with matching mortality profiles, where it is reasonable to assume that migration is negligible for children under the age of 5 years. The highest child mortality was concentrated in the African region, followed by Asia and the Western region. However, steady progress in child mortality trends was concentrated in low-income countries. This simple method demonstrated that child mortality has significantly improved in high-income countries, followed by middle- and low-income countries. To reduce the under-5 mortality rates even further in these 52 countries, there is a need to accelerate equitable plans and policies related to child health to promote children's longevity and survival.

• 농촌지도와개발
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• 제19권4호
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• pp.1137-1170
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• 2012

귀농 귀촌자의 동기, 태도, 행태에 대한 이해를 위해 설문조사를 통해 귀농 귀촌자를 귀농 귀촌동기에 따라 유형화하였다. 요인분석 결과 귀농 귀촌자는 5개의 귀농 귀촌동기 차원을 가지고 있었다. 5개의 귀농 귀촌동기 차원은 '경제적 성공 추구', '전원적 삶 추구', '가족생활 추구', '도시생활회피 추구', '영농승계 추구'이다. 이 중에서 전체변량을 가장 많이 설명하는 귀농동기 차원은 '경제적 성공 추구'와 '전원적 삶 추구'로 나타났다. 이 같은 결과는 귀농자들은 '전원적 삶 추구'를 가장 중요한 귀농동기로 고려하고 있음을 나타낸다. 귀농 귀촌동기에 따라 귀농 귀촌자를 유형화하기 위하여 귀농 귀촌동기 차원에 기초하여 군집분석을 실시하였다. 군집분석결과 4개의 군집으로 구분되었다. 4개의 군집은 '기업가형', '소규모창업형', '전원형', '영농승계형' 이다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3757-3760
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• 2013

MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells. Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR-485 might play an important role in breast cancer by suppressing cell proliferation and migration.