• Title/Summary/Keyword: microscopic system

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Scanning Electron Microscopic Observation of Human Skin Replica

  • Rhyu, Yeon-Seung;Chung, Ye-Ji;Uhm, Chang-Sub
    • Applied Microscopy
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    • v.40 no.4
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    • pp.267-270
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    • 2010
  • The skin is the largest organ of the integument system whose surface is closely related with many physiological and pathological conditions. Various methods are used to understand the structural and functional status of human skin. We would like to present usefulness of scanning electron microscopic (SEM) observation of skin replica and its significance of training module for a novice. The silicon replicas from several regions of the body (hand, finger, forearm, lip, and face) were casted by applying Exafine$^{(R)}$ mixture. The positive replicas were prepared by applying EPON 812 mixture on negative silicon replicas. Some of the negative silicon replicas were cut with a razor blade and surface profiles were observed. The negative and positive replicas were coated with platinum and were observed under the scanning electron microscope. We could investigate the detailed structures of the human skin surface without any physical damage to the subject. The positive replicas depicted real surface structure of the human skin vividly. The cross sectional view of the negative silicon replicas provided surface profile clearly. The scanning electron microscopic observation of the human skin replicas would be useful to study skin surface structures and to evaluate medical and esthetical applications.

Analysis of Spatial Characteristics of Business-Type-Changed Parcel in Hongik-University Commercial Area, Seoul - Focused on the View Point of Commercial Gentrification - (서울시 홍대상권 내 업종변화 필지의 공간적 특성 분석 - 상업 젠트리피케이션의 관점에서 -)

  • Kim, Dongjun;Kim, Kijung;Lee, Seungil
    • Journal of Korea Planning Association
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    • v.54 no.2
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    • pp.5-16
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    • 2019
  • The purpose of this study is to analyze the spatial characteristics of business-type-changed parcel in the Hongik-University commercial area, from the view point of commercial gentrification. A commercial gentrification occurs through a business-type-change in a spatial basic unit (microscopic spatial unit such as parcel) of an area which has not been considered in relavent policies and research. So, this study analyzed the spatial characteristics of business-type-changed parcels using the Cox's proportional hazard regression model. The main results of this study are as follows. First, as new developments in the adjacent area occur, the business-type-change probability increases. Second, by the commercial area division, the business-type-change probability is different. Finally, the accessibility is better, the probability is higher. These results could suggest that a consideration of the spatial characteristics form microscopic viewpoint is necessary to understand the commercial gentrification. And these could be used as basic data for a gentrification diagnostic and management system, which can predict gentrification from the view point of business-type-change on the basis of a parcel.

Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing (고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법)

  • Choi, Yun-Joung;Lee, Kyung-Hwan;Je, A-Reum;Chae, Hee-Su;Jang, Ji-Hoon;Lee, Eun-Ji;Kweon, Hee-Seok
    • Applied Microscopy
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    • v.42 no.2
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    • pp.111-114
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    • 2012
  • The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

Endoscopic Fluorescence Angiography with Indocyanine Green : A Preclinical Study in the Swine

  • Cho, Won-Sang;Kim, Jeong Eun;Kim, Sae Hoon;Kim, Hee Chan;Kang, Uk;Lee, Dae-Sic
    • Journal of Korean Neurosurgical Society
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    • v.58 no.6
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    • pp.513-517
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    • 2015
  • Objective : Microscopic indocyanine green (ICG) angiography is useful for identifying the completeness of aneurysm clipping and the preservation of parent arteries and small perforators. Neuroendoscopy is helpful for visualizing structures beyond the straight line of the microscopic view. We evaluated our prototype of endoscopic ICG fluorescence angiography in swine, which we developed in order to combine the merits of microscopic ICG angiography and endoscopy. Methods : Our endoscopic ICG system consists of a camera, a light source, a display and software. This system can simultaneously display real-time visible and near infrared fluorescence imaging on the same monitor. A commercially available endoscope was used, which was 4 mm in diameter and had an angle of $30^{\circ}$. A male crossbred swine was used. Results : Under general anesthesia, a small craniotomy was performed and the brain surface of the swine was exposed. ICG was injected via the ear vein with a bolus dose of 0.3 mg/kg. Visible and ICG fluorescence images of cortical vessels were simultaneously observed on the display monitor at high resolution. The real-time merging of the visible and fluorescent images corresponded well. Conclusion : Simultaneous visible color and ICG fluorescent imaging of the cortical vessels in the swine brain was satisfactory. Technical improvement and clinical implication are expected.

Studies on the Mechanism of Nonastringency and Production of Tannin in Persimmon Fruits -Part IV. Microscopic Observation and Change of Tannin contents in Persimon Leaves during Growth- (감 과실(果實)의 탄닌 물질(物質) 생성(生成) 및 탈삽기구(脫澁機構) -제사보(第四報). 감 엽(葉)의 탄닌 함량(含量)의 변화(變化) 및 현미경적(顯微鏡的) 관찰(觀察)-)

  • Sohn, Tae-Hwa;Seong, Jong-Hwan
    • Applied Biological Chemistry
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    • v.25 no.4
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    • pp.201-205
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    • 1982
  • As a series of study on the removal of astringency and production of tannin substances, this experiment was conducted to investigate changes of tannin contents and microscopic observation on leaves of persimmon(Diospyros kaki L.). Changes of tannin contents of leaves and fruits in Daegu Bansi(astringent variety) were of similar tendency in content of total and soluble tannin during growth. It seemed therefore that tannin contents of leaves in astringent variety were closely concerned with tannin of fruits. But Fuyu(sweet variety) was different from each other during its growth. Histological form of leaves in microscopic observation had similar tendency between astringent and sweet varieties. It was observed that tannin substances were contained in epidermis cells of levels, that tannin substances were transfered in phloems of nerves system, and that tannin cell existed too in the vicinity of nerves system.

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Implementation of Microscopic Pedestrian Simulation by using NetLogo (NetLogo를 이용한 미시적 보행시뮬레이션의 구현)

  • Jang, Sung Min;Rhee, Jong Ho;Oh, Seung Hwoon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.2D
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    • pp.119-125
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    • 2010
  • Transportation system improved with rapid economic growth has helped human being a lot. However, the system has been also created social problems. The fast increasing auto ownership has not only increased transportation accidents but decreased air quality rapidly. One of the serious problems in transportation is that transportation policies have disregarded human factor, especially pedestrian environment. This research has developed microscopic pedestrian simulation with Netlogo. Netlogo is a very useful program for simulating complex systems such as pedestrian movements. The simulated pedestrian movements has been compared with actual surveyed pedestrian movements on a sidewalk. The comparison has been showed that the simulation program can be used for analyzing various pedestrian environments. Also, the impacts of bollard on the sidewalks have been analyed with the simulation program.

Differential Interference Contrast Microscopic Module Using a Polarization Grating for Quantitative Phase Imaging (편광 격자 기반 정량적 위상 이미징을 위한 미분 간섭 현미경 모듈 개발)

  • Jin Hee Cho;Ki-Nam Joo
    • Korean Journal of Optics and Photonics
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    • v.34 no.6
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    • pp.261-268
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    • 2023
  • We propose a compact differential interference contrast microscopic module, which enables snapshot measurements for quantitative phase imaging. The proposed module adopts the lateral shearing interferometric principle, which can obtain self-interference without a reference. Due to the absence of the reference, the system is more stable than the typical interferometric systems. It uses a polarization grating to generate two laterally shifted wavefronts based on its birefringence and polarizing beam-splitting characteristics. Furthermore, the use of a polarization camera does not require sequential measurements for the phase extraction. In the experiments, we observe and measure the timely varying changes of various specimens to verify the system performance with the bright field images and phase contrast images. Because the proposed microscopic module also has the merit of being adaptable to typical microscopy instead of using an imaging camera, it can conveniently replace conventional contrast microscopy.

Performance Analysis of DS/SSMA DPSK Communication System with Diversity and Channel Coding in Mobile Radio Environment (이동 무선 채널에서 다이버시티와 채널 부호를 사용한 DS/SSMA DPSK 통신 시스템의 성능 분석)

  • 강병권;조병진;황금찬
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.30A no.7
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    • pp.1-11
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    • 1993
  • The bit error rates of DS/SSMA DPSK system with diversity and FEC channel coding in Rayleigh fading and log-normal shadowing channel are evaluated. Microscopic postdetection diversity and macroscopic selection diversiity are used to mitigate the effects of Rayleigh fading and log-normal shadowing, respectively. The numerical results show that the composite use of diversity and channel coding is very efficient for improving system performance compared to their respective uses.

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Optimum Speed Simulation for Electronic Toll Collection (Electronic Toll Collection 운영속도 시뮬레이션)

  • Sin, Heung-Gweon;Nam, Doohee
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.3
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    • pp.87-92
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    • 2013
  • This study is to analyze the impacts of different High-Pass lane speed limits. Paramics, microscopic simulation software, was used to perform microscopic simulations. For the simulations, Incheon toll gate traffic count data was used. The Paramics simulation results show that higher High-Pass speed limit (70Km/h) produced better traffic flow condition and less air pollution cost compared to existing High-Pass speed limit (30Km/h).

Finite Step Method for the Constrained Optimization Problem in Phase Contrast Microscopic Image Restoration

  • Adiya, Enkhbolor;Yadam, Bazarsad;Choi, Heung-Kook
    • Journal of Multimedia Information System
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    • v.1 no.1
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    • pp.87-93
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    • 2014
  • The aim of microscopic image restoration is to recover the image by applying the inverse process of degradation, and the results facilitate automated and improved analysis of the image. In this work, we consider the problem of image restoration as a minimization problem of convex cost function, which consists of a least-squares fitting term and regularization terms with non-negative constraints. The finite step method is proposed to solve this constrained convex optimization problem. We demonstrate the convergence of this method. Efficiency and restoration capability of the proposed method were tested and illustrated through numerical experiments.

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