• Applied Biological Chemistry
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• 제25권4호
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• pp.201-205
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• 1982

감 과실(果實)의 탈삽기구(脫澁機構) 및 탄닌생성에 관한 조사의 일환으로 감엽(葉)의 탄닌함량의 변화 및 현미경적 관찰의 결과 삽시(澁枾)에서는 엽(葉)과 과실(果實)의 탄닌함량의 변화 경향이 유사하였으나, 감시(甘枾)에서는 많은 차이가 있었다. 엽(葉)의 탄닌함량 변화는 품종간에 큰 차이가 없었다. 엽(葉)의 상피세포(上皮細胞)는 과실(果實)의 탄닌세포와 같이 다량의 탄닌물질을 함유하고 있었으며 유관속(維管束) 부근에도 탄닌세포가 존재하였다. 사관부내(篩管部內)에는 탄닌물질이 이동하고 있었다. 이상의 결과 감엽(葉)의 탄닌물질과 과실(果實)의 탄닌물질은 밀접한 관계가 있음을 알 수 있었다.

• Journal of Periodontal and Implant Science
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• 제37권2호
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• pp.251-263
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• 2007

The present study was performed to evaluate the effects of Tetracycline-HCI on the microstructure change of SLA implant surface according to application time. In the Tetracycline-HCI group, 6 implants were rubbed with sponges soaked $50mg/m{\ell}$ Tetracycline-HCI solution for O.5min., lmin., 1. 5min., 2min., 2.5min. and 3min. In the saline group. another 6 implants conditioned with sponges soaked saline using same methods. One implant wasn't conditioned anything. Then, the changes of surface roughness values were evaluated by optical interferometer & specimens were processed for scanning electron microscopic observation. The results of this study were as follows: 1. In both Tetracycline-HCI group & saline group, there are no significant differences between surface roughness values before & after surface detoxification. And in scanning electron microscopic observation. there are slightly changes of implant surface structures but this changes were not significant by comparison with no treatment implant surface. 2. In the changes of surface roughness values & the scanning electron microscopic observation, there were no significant differences between saline & Tetracycline-HCI groups. In conclusion, the detoxification with $50mg/m{\ell}$ Tetracycline-HCI within 3 minutes can be applied for treatment of peri-implantitis in SLA surface implants. without surface microstructure changes.

• 한국건설순환자원학회논문집
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• 제11권2호
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• pp.145-152
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• 2023

본 연구에서는 알칼리 이온의 침출을 완화하기 위해 전기화학적 처리로 100일 동안 수중 환경에 침지된 시멘트 페이스트의 표면을 현미경으로 관찰하였다. 계면 영역에서의 미수화물 입자와 공극률을 정량적으로 평가하기 위하여 주사 전자 현미경을 통해 후방 산란 전자(BSE) 이미지를 얻었다. 그 결과, 전기화학적 처리에 의해 표면부의 공극률이 크게 감소한 반면, 전하 상태에서 제한된 수화반응에 의해 미수화 입자는 다소 증가한 것으로 나타났다. 전기화학적 처리에서, C-S-H 겔에 존재하는 Ca²⁺ 이온은 공극수 내의 OH^- 이온과 Ca(OH)₂ 형태로 침전되어 C-S-H 겔을 낮추면서 동시에 Ca(OH)₂를 증대시킬 수 있다. 실질적으로 알칼리 침출에 대한 위험성은 전기화학적인 처리 하에서 제한된 이온화 매트릭스에 의해 감소될 수 있다.

• 생명과학회지
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• 제9권2호
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• pp.127-135
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• 1999

자생식물 중 절화, 분화 및 화단용으로서 가치가 높고, 한약으로서 이용 가능한 용담의 발아성과 발아과정중 형태형성에 관계하는 식물체의 특성을 이해하기 위해 각 부분의 내부조직을 광학현미경과 주사전자현미경으로 관찰한 결과는 다음과 같다. 용담은 채종직 후 발아하여 휴면이 없거나 극히 짧았다. 성숙종자는 미숙종자보다 높은 발아율을 보였고, 미숙종자는 저장기간이 경과할수록 수명이 단축되었다. 종자의 수명은 190일 정도였다. 발아과정중 수반되는 형태형성을 관찰한 결과, 치상직 후에 종자는 수분을 흡수하여 종피가 팽창하였으며, 2일 후에는 유근이 출현되었다. 치상 4일째에는 유근에서 근모가 발생되었고, 파종 6일 후에는 자엽이 전개되었다. 치상 8∼10일 후에는 완전한 식물체로 발육되었다. 잎의 및의 조직을 검경한 결과 중앙맥과 지맥을 구별할 수 있었으며, 관다발이 잘 발달되어 있고, 단층의 표피조직과 책상조직 및 해면조직등이 관찰되었다. 책상조직은 대개 1열의 세포층으로 되어 있었다. 기공은 잎의 이면에만 분포하였고, 기공의 형태는 공변세포 주위에 부세포가 존재하지 않는 불규칙형으로 배열되어 있었다. 줄기 조직은 단층의 표피조직과 유조직으로 구성되어 있는 피층, 그리고 원형의 대를 이루고 있는 유관속을 관찰 할 수 있었다. 줄기의 유관속 조직배열은 둥근 원형의 띠모양을 나타내었다.

• Applied Microscopy
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• 제28권1호
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• pp.107-119
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• 1998

This study was designed to investigate the appearence and the characteristics of the apoptotic cells and the process of the joint cavity formation in mouse knee joint. Fetal mouse knee joints from 15 to 19 days of gestation were used. Paraffin-embedded serial sections, stained with H & E for light microscopic observation, Epon 812 embedded thin sections for electron microscopic observation and Lowicryl HM 20 embedded thin sections for immune-electron microscopic observation were prepared. Monoclonal antibodies to $\beta-tubulin$ and polyclonal antibodies to tissue transglutaminase were used for immune-electron microscopic study. The results obtained were as follows. 1. At 15 days of gestation, blood vessels, which have invaded in the mesenchymal cells, were present in the synovium, to form the joint cavity in the future. 2. At 16 days of gestation, the joint cleft was first appeared and several RBCs were present in the joint cleft. The invasion of blood vessels into the joint cleft was continuing, and apoptotic cells were present in the inner cell layer, adjacent to the joint cleft. Necrotic cells were also present in the outer cell layer; they were present 18 days of gestation, but apoptotic cells did not appear after 17 days of gestation. 3. In the apoptotic cells, transglutaminase were localized around vacuoles and the marginal site of the cytoplasm. 4. In the apoptotic cells, tubulin was around the endoplasmic reticulum and the marginal site of the cytoplasm. In the late stage of apoptotic cells, tubulin was localized diffusely in the cytoplasm. Tubulin was also strongly labeled around in the cytoplasm of the neighboring cell at which the apoptotic body was phagocytosed. Tubulin labeled particles were apparently increased in the seperated apoptotic bodies. On the basis of the above findings, it is proposed that during the development of the mouse knee joint, blood vessel invasion first occurs and then apoptosis and cell necrosis follow it. In the apoptotic cell, present in the synovium of the developing knee joint of the mouse. it is suggested that the redistribution of tubulin is associated with apoptotic process. And transglutaminase overexpressed in the apoptotic cell.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1993년도 정기총회 및 추계학술대회 논문집 학회본부
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• pp.356-358
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• 1993

With a focused laser beam, we make a microscopic change on a thin film, and organize the overall system that can observe the change with a microscope. This system utilizes the energy of a laser beam which is focused by a simple lense system. That is a laser beam which has its waist at working distance of a lense. The microscopic change induced by the energy of laser beam focused through a lense can be observed in real time. The experiment with this system shows that the 10mW He-Ne 632.8nm laser can change the black vinyl instantaneously.

• Applied Microscopy
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• 제25권3호
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• pp.33-39
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• 1995

To compare the quality of ultrastructural preservation of Scanning Electron Microscopic specimens dried with different methods; pure air-drying, air-drying with using Tetramethylsilane(TMS), four kind of air-drying using Hexamethyldisilazane(HMDS) and critical point drying(CPD), we conducted scanning electron microscopic observation on liver, skeletal muscle and intestinal tissues from laboratory rat treated with each method. In pure air drying group, severe distortion of tissue surface was observed, and in HMDS treated group, only liver tissue showed slight distortion. But in TMS treated group, each tissue showed a good presentation comparable to CPD group. The results suggest that the method of air-drying using TMS may be the former is less expensive and simple be and also time-saving.

• Tribology and Lubricants
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• 제5권1호
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• pp.36-43
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• 1989

Scanning electron microscopic observations were carried out on the worn surface and the wear debris of ceramic materials to investigate the wear mechanism of those in dry rolling contact. It was found from the scanning electron microscopic observations that the wear in ceramic materials is related to brittle fracture and has two types of wear mechanisms, small scale wear and larvae scare wear. Plate-like wear debris were created from the initial surface cracks and defects. The small scale wear was related to real contact area and large scale wear was related to HertzJan contact area. A wear model was proposed on the basis of scanning electron microscopic observations.

• Applied Microscopy
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• 제25권1호
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• pp.86-95
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• 1995

This study was accomplished to investigate the ultrastructure of mucous glands in dorsal skin of frog (Rana catesbeiana) by means of scanning and transmission electron microscopes. The dorsal skin of Rana catesbeiana is composed of epidermis and dermis. The cutaneous mucous glands consist of inner glandular epithelial cells and outer myoepithelial cells. Glandular epithelial cells are divided into four types by the microscopic ultrastructure; ER-rich cell, round secretory granule-containing cell, foam-like granule mass-containing cell, mitochondria-rich cell. Myoepithelial cell has a long elliptical nucleus and filled with fibrous materials in the cytoplasm. As a result of scanning microscopic observation, the surface of dorsal skin is covered with cutaneous protrusions. The opening sites of the mucous glands are irregularly distributed in dorsal skin.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 1998년도 Proceedings of International Symposium on Remote Sensing
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• pp.14-19
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• 1998

The MUBEX (MUtsu Bay sea surface temperature validation EXperiment) campaign has been held from 1995 to 1997 in summer. During the MUBEX campaign, a thermal infrared camera (TIC) installed on a research vessel, which was also equipped with other various observation devices, was intensively used to observe microscopic structure of sea skin surface temperature (SSST) behavior. We have now a total number of 500,000 images observed by the TIC under various weather conditions, i.e., very calm or wavy sea condition, and clear, patchy or cloudy sky condition. In this paper, we show typical SSST patterns observed by the TIC, and describe the result of statistical analysis of SSST.