Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Comparison of Scanning Electron Microscopic Specimens Dried with Different Methods

건조기법을 달리한 SEM 시료상의 비교검토

  • Park, Chang-Hyun (Department of Veterinary Medicine, College of Animal Husbandary, Kon Kuk University) ;
  • Jang, Byung-Joon (Department of Veterinary Medicine, College of Animal Husbandary, Kon Kuk University) ;
  • Cho, Kang-Yong (Department of Pathology, College of Medicine, Korea University)
  • 박창현 (건국대학교 축산대학 수의학과) ;
  • 장병준 (건국대학교 축산대학 수의학과) ;
  • 조강용 (고려대학교 의과대학 병리학교실)
  • Published : 1995.09.01
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Abstract

To compare the quality of ultrastructural preservation of Scanning Electron Microscopic specimens dried with different methods; pure air-drying, air-drying with using Tetramethylsilane(TMS), four kind of air-drying using Hexamethyldisilazane(HMDS) and critical point drying(CPD), we conducted scanning electron microscopic observation on liver, skeletal muscle and intestinal tissues from laboratory rat treated with each method. In pure air drying group, severe distortion of tissue surface was observed, and in HMDS treated group, only liver tissue showed slight distortion. But in TMS treated group, each tissue showed a good presentation comparable to CPD group. The results suggest that the method of air-drying using TMS may be the former is less expensive and simple be and also time-saving.

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