• Title/Summary/Keyword: microscopic analysis

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Differences of Physical, Mechanical and Chemical Properties of Korean Red Pine(Pinus densiflora) Between Old and New Wood (소나무 고목재와 건전재의 물리, 기계, 화학적 특성 차이)

  • Shim, Kug-Bo;Lee, Do-Sik;Park, Byung-Soo;Cho, Sung-Taig;Kim, Kwang-Mo;Yeo, Hwan-Myeong
    • Journal of Korea Foresty Energy
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    • v.25 no.2
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    • pp.1-8
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    • 2006
  • The physical, mechanical and chemical properties of old and new Korean red pine (Pinus densiflora) were analyzed. The old woods were from dismantled timbers of Bonjungsa temple. The crystallized resin in the latewood was observed by microscopic analysis. Also, reduction of specific gravity, occurrence of microscopic cleavage of tracheid was observed in the old wood. The angle of microscopic cleavage of tracheid is estimated with the same angle of micro-fibril angle of 52 layer. The bending, compression and shear strength of old world were decreased about 35-27% than those of new wood. Dynamic modulus of elasticity measured by ultrasonic nondestructive test has the tendency of reducing by the time elapse of the wood usage. Therefore, deterioration of wood could be measured by reduction of specific gravity and dynamic MOE. The static MOE and mechanical properties of old wood could be predictable by measuring dynamic MOE in the longitudinal direction. Extractives of the old wood in 1-% NaOH solution are larger quantity than new wood. Therefore the decay of the wood could be evaluated by analyzing the chemical compound, especially 1-% NaOH solution. The results of this research could be used for understanding and prediction of the changing properties with elapsing time of wood.

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Experimental Examination of the Beer's law for Quantitative Electron Tomography (정량적 전자토모그래피를 위한 Beer's law의 실험적 검증)

  • Kim, Jin-Gyu;Song, Kyung;Lee, Su-Jeong;Jou, Hyeong-Tae;Kim, Youn-Joong
    • Applied Microscopy
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    • v.40 no.2
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    • pp.117-123
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    • 2010
  • This study has examined experimentally the Beer's law which is a precondition for quantitative electron tomography. We used carbon support film and latex spheres, which have similar absorption coefficients with biological samples, as the test samples to take a tilt-series of images for electron tomography. First, the 3D information of carbon film and latex spheres was obtained by electron tomography. Then, the regression analysis on the relationship between the intensities of the incident and the transmitted beams in a tilt series was carried out to examine the Beer's law. The regression results with RMS error of 0.976 show the linear intensity variations of the transmitted beam as the tilt angles were increased. In addition, the relative absorption coefficients of carbon support film and latex spheres calculated experimentally through the Beer's law were 1.71 (5) and 2.67 (6)/${\mu}m$, respectively. The absorption coefficients remained constant within a full tilt range. Therefore, it is expected that quantitative electron tomography could be performed for biological samples by applying Beer's law provided the exact intensity of incident beam can be obtained under the thoroughly controlled experimental conditions.

Biological Activity and Chemical Characteristics of Cordyceps militaris Powder Fermented by Several Microscopic Organisms (발효 동충하초의 유용성분 및 생리 활성 작용)

  • Ahn, Hee-Young;Park, Kyu-Rim;Yoon, Kyoung-Hoon;Lee, Jae-Yun;Cho, Young-Su
    • Journal of Life Science
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    • v.25 no.2
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    • pp.197-205
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    • 2015
  • The comparative effects of the fibrinolytic action, antioxidative activity, and tyrosinase inhibition of Cordyceps militaris powder and fermented Cordyceps militaris powders were investigated using several microscopic organisms. The nutritional components such as phenolic compounds, flavonoids, and minerals were also measured. The total phenolic compounds and flavonoid concentrations were highest in the Cordyceps militaris powder fermented by Aspergillus oryzae. Major minerals were K, Ca, Mg, and Zn. Native polyacrylamide gel electrophoresis (native-PAGE) analysis of the total protein patterns of Cordyceps militaris powder and fermented Cordyceps militaris powders revealed slight varietal differences. Fibrinolytic activity was highest in the Cordyceps militaris powder fermented by Bacillus subtilis and Aspergillus kawachii. The DPPH radical scavenging activity was slightly stronger in the powder fermented by Monascus purpureus; however, these samples all exhibited a relatively low activity when compared with butylated hydroxytoluene (BHT). Tyrosinase inhibition activity was stronger in the powder fermented by Aspergillus oryzae than in unfermented powder. These results may provide basic data for understanding the biological activities and chemical characteristics of Cordyceps militaris powder fermented by several microscopic organisms for the development of functional foods.

Comparison of Delay Estimates for Signalized Intersection (신호교차로 지체 산정 비교)

  • Jo, Jun-Han;Jo, Yong-Chan;Kim, Seong-Ho
    • Journal of Korean Society of Transportation
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    • v.23 no.1
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    • pp.67-80
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    • 2005
  • In this paper, the primary objective of the research are to review the methods currently avaliable for estimating the delay incurred by vehicles at signalized intersections. The paper compares the delay estimates from a deterministic queueing model, a model based on shock wave theory , the steady-state Webster model, the queue-based models defined in the 1994 and 2001 version of the High way Capacity Manual, in addition to the delays estimated from the TRANSYT-7F macroscopic simulation and NETSIM microscopic simulation. More especially, this paper is to compare the delay estimates obtained using macroscopic and microscopic simulation tools against state-of-the practice analytical models that are derived from deterministic queueing and shock wave analysis theory. The results of the comparisons indicate that all delay models produce relatively similar results for signalized intersections with low traffic demand, but that increasing differences occur as the traffic demand approaches saturation. In particular, when the TRANSYT-7F and NETSIM are compared, it is highly differences as approach for traffic condition to over-saturation. Also, the NETSIM microscopic simulation is the lowest estimates among the various models.

Fluorescence Molecular Imaging

  • Choi, Heung-Kook;Ntziachristos, Vasilis;Weissleder, Ralph
    • Proceedings of the KSMRM Conference
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    • 2004.09a
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    • pp.23-32
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    • 2004
  • The chemotherapy sensitive Lewis lung carcinoma (LLC) and chemotherapy resistant Lewis lung carcinoma (CR-LLC) tumors concurrently implanted in mice, and compare these findings with histological macroscopic observations against 3D reconstruction of Fluorescence Molecular Tomography (FMT) preformed in vivo on the same animals. For the 3D image reconstruction we used 32 laser source images, a flat image and 3D surface rendering that confused for 3D Fluorescence Molecular Imaging (FMI). A minimum of ten tissue sections were analyzed per tumor for quantification of the TUNEL-positive cells, cell-associated Cy5.5-Annexin and vessel-associated Alexa Fluor-Lectin. These are useful apoptosis and angiogenesis markers, and they serve as validation experiments to data obtained in vivousing a Cy5.5-Annexin V conjugate injected intravenously in chemotherapy-treated animals carrying the tumors studied histologically. We detected higher levels of apoptosis and corresponding higher levels of Cy5.5 fluorescence in the LLC vs. the CR-LLC tumors according to tissue depth and these findings confirm that in vivo staining with the Cy5.5-Annexing conjugate correlates well with in vitro TUNEL staining and is consistent with the higher apoptotic index expected from the LLC line. There appeared to be 1.38% more apoptosis for LLC than CR-LLC. Consequently there is good correlation between the histology results and in vivo fluorescence-mediated optical imaging. In conclusion the apoptotic images of 3D FMI were validated by microscopic histological image analysis. This is a significant result for the continuous progress of fluorescence 3D imaging research.

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Microscopic Approach of Mass Involving Neurovascular Pedicle in the Hand (신경혈관 줄기를 침범한 수부종양의 미세현미경적 접근)

  • Hwang, Min-Kyu;Hwang, So-Min;Lim, Kwang-Ryeol;Jung, Yong-Hui;Song, Jennifer Kim
    • Archives of Reconstructive Microsurgery
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    • v.21 no.2
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    • pp.86-91
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    • 2012
  • Purpose: Mass can compress around tissue and cause deviation of normal anatomical structures. Often, mass grows toward neurovascular pedicle and encircles depending on the nature of mature mass. Neglecting neurovascular involvement of the mass is a serious problem not to be overlooked. Authors have performed microscopic approach regarding mass involving the neurovascular pedicle in the hand. Materials and Methods: From January 2007 through February 2012, retrospective analysis for nine cases of mass involving neurovascular pedicles was done. Patients were evaluated preoperatively by ultrasonography or MRI and checked intraoperative finding. Masses were evaluated by site, preoperative evaluation, involved neurovascular pedicle, histopathologic diagnosis, complication, and recurrence. Results: The site of mass involving neurovascular pedicles was 4 cases on the wrist, 2 cases on the palm, 2 cases on the finger, 1 case on the hand dorsum. Involved neurovascular pedicles were 3 radial arteries and nerves, 3 proper digital arteries and nerves, 1 radial artery, 1 superficial branch of radial nerve, 1 common digital artery and nerve. The histopathologic diagnosis of mass were 3 ganglions, 2 giant cell tumors, 2 epidermal cysts, 1 fibroma, and 1 benign spindle tumor. There were 2 cases of recurrence and secondary excisions were performed. Conclusion: Neurovascular pedicle injury can lead to serious complication like sensory and motor disorders, distal part ischemia, and so on. In case of mass suspected neurovascular invasion, accurate preoperative evaluation such as ultrasonography or MRI is necessary. To prevent any neurovascular related complication during mass excision, delicate surgical technique using a microscope becomes essential.

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Flying Characteristics of Running Tape above Rotating Head (II) -Experimental Analysis- (회전헤드에 대한 주행테이프의 부상특성 (II) -실험해석-)

  • 민옥기;김수경
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.15 no.1
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    • pp.107-119
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    • 1991
  • This dissertation analyzes the running mechanism of flexible and thin tape above rotating head through the experiment. The scope of study is confined to measure the vertical deformation of running tape under hydrodynamic pressure invoking phenomena of elasto-hydrodynamic lubrication between the protruded bump on a rotating cylinder ad the running tape. Experimental system is devised to measure the vertical deflection of the running tape by opto-electronical displacement gauge, which enables to detect microscopic surface deflection of high frequency. Thorough the tests of small specimens of groove and bump, the accuracy and reliability of this experimental method is confirmed and achieved an accuracy within 5%(2.mu.m) error for the microscopic deflection with high frequency. In experimental works, the effects of bump size on flying characteristics of the tape were evaluated and examined. For the vertical deformation of the running tape. the numerical results and its trend agree qualitatively with the experimental ones.

The Coexisting Thyroid Carcinoma in Graves' Disease (Graves'병과 갑상선암)

  • Cho Tae-Hyung
    • Korean Journal of Head & Neck Oncology
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    • v.11 no.2
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    • pp.125-131
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    • 1995
  • The authors present 15 cases in which the diagnosis of thyroid cancer was established pathologically among 300 cases of Graves' disease diagnosed clinically at Chosun University Hospital, from January 1982 to December 1994. These cases were analyzed in order to establish guidelines for prophylactic node dissection as part of the initial management of thyroid cancer in patients with Graves' disease. The analysis revealed the following: 1) The average age of the 15 patients was 34.5 years and the male: female ratio was 1 : 4.0. 2) In 8 of the 15 cases(53.5 %) the occult thyroid carcinoma measured less than 1.5cm. 3) The degree of invasivensess manifested in these fifteen cases may be summarized as follows: In Group 1(6 cases) there was absence of microscopic capsular invasion and of lymphnode metastasis. In Group 11(4 cases) threre was microscopic capsular invasion but absence of lymphnode metastasis: In Group III(4 cases) there was either extrathyrodal soft tissue invasion or regional lymph node metastasis: and in Group IV(1 case) there was lymphnode invasion and distant metastasis. 4) Thirteen patients underwent either subtotal or near total thyroidectomy, and 2 patients underwent total thyroidectomy. Seven patients underwent some type of neck dissection, as follows: anterior compartment dissection in one of the cases in Group I; functional neck dissection in two cases and jugular node dissection in one case in Group II; and anterior compartment dissection in one case and modified radical neck dissection in two cases in Group III. 5) The author propose the following guidelines for prophylactic initial node dissection when a unexpected coexisting thyroid carcinoma in encountered on the frozen section during the surgical management of Graves' disease; Group I cases do not require initial neck dissection in group II, anterior compartment dissection in sufficient. In Group III, either jugular node dissection or functional neck dissection should be performed, and followed by postoperative Ra$^{131}$I therapy, Group IV requires Ra$^{131}$I therapy with or without modified radical neck dissection depending in the patient's condition.

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A Review of Emissions Studies for Transportation Engineering (교통환경분야의 국내외 연구동향 및 시사점 (차량배출량 관련 연구를 중심으로))

  • Gang, Jong-Ho;Lee, Cheong-Won
    • Journal of Korean Society of Transportation
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    • v.25 no.6
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    • pp.7-18
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    • 2007
  • There are few studies on air pollution due to vehicle emissions in spite of the importance of this field. Therefore, this study describes trends and suggests implications through analysis relating to existing emissions research. This study has been divided into three areas. The first part is about estimating vehicle emissions. In this part, the authors analyze limits in ways of calculating emissions in the existing macroscopic view and then suggest the development of a model for calculating emissions considering velocity and acceleration. These variables are a function of traffic and individual driving behavior in the microscopic view. The second part is about management techniques for reducing vehicle emissions. The traffic management techniques for reducing vehicle emissions should conform to regional characteristics. The final part is about traffic operation for reducing vehicle emissions. The authors suggest the development of a micro-simulator and then the development of strategies for traffic operation. It is necessary to design better models estimating emissions and then, using real time data, to make a monitoring system simulating emission rates. This study serves as a literature review to make a foundation for further research about emissions research for transportation engineering.

A Case of Lupus Nephritis with Positive Antiphospholipid Antibodies, Initially Detected Through Analysis of Urinary Mass Screening (학교신체검사에서 발견된 항인지질 항체 양성 낭창성 신염 1례)

  • Lee Taek-Jin;Choi Min Sook;Lee Young-Mock;Kim Ji-Hong;Kim Pyung-Kil;Jeong Hyeon-Joo
    • Childhood Kidney Diseases
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    • v.5 no.2
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    • pp.219-224
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    • 2001
  • Antiphospholipid syndrome is a thrombotic disorder characterized by the association of arterial and venous thrombosis with the antibodies directed toward phospholipids. The presence of these antibodies in systemic lupus erythematosus(SLE) has been shown to be related to several clinical and analytical alterations. We experienced one case of lupus nephritis with positive antiphospholipid antibodies in a 10-year-old girl whose chief complaint was persistent microscopic hematuria. We report this case with a brief review of related literatures. (J. Korean Soc Pediatr Nephrol 2001 ;5 : 219-24)

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