• Title/Summary/Keyword: microscope image processing

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A Development of the Inference Algorithm for Bead Geometry in the GMA Welding Using Neuro-fuzzy Algorithm (Neuro-Fuzzy 기법을 이용한 GMA 용접의 비드 형상에 대한 기하학적 추론 알고리듬 개발)

  • Kim, Myun-Hee;Bae, Joon-Young;Lee, Sang-Ryong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.27 no.2
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    • pp.310-316
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    • 2003
  • One of the significant subject in the automatic arc welding is to establish control system of the welding parameters for controlling bead geometry as a criterion to evaluate the quality of arc welding. This paper proposes an inference algorithm for bead geometry in CMA Welding using Neuro-Fuzzy algorithm. The characteristic welding parameters are measured by the circuit composed of hall sensor, voltage divider tachometer, etc. and then the bead geometry of each weld pool is calculated and detected by an image processing with CCD camera and a measuring with microscope. The relationships between the characteristic welding parameters and the bead geometry have been arranged empirically. From the result of experiments, membership functions and fuzzy rules are tuned and determined by the learning of neural network, and then the relationship between actual bead geometry and inferred bead geometry are concluded by fuzzy logic controller. In the applied inference system of bead geometry using Neuro-Fuzzy algorithm, the inference error percent is within -5%∼+4% in case of bead width, -10%∼+10% in bead height, -5%∼+6% in bead area, -10%∼+10% in penetration. Use of the Neuro-Fuzzy algorithm allows the CMA Welding system to evaluate the quality in bead geometry in real time as the welding parameters change.

Droplet Geometry and Its Volume Analysis (기름방울 형상 및 그 체적 분석법)

  • Yoon, Moon-Chul
    • Tribology and Lubricants
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    • v.24 no.6
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    • pp.320-325
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    • 2008
  • The recent industrial application requires technical methods to get the cutting fluid droplet surfaces in particular from the viewpoint of topography and micro texture. To characterize the surface topography of droplet, the combination of the confocal laser scanning microscope (CLSM) and wavelet filtering is well suited for obtaining the droplet geometry encountered in tribological research. This technique indicates a better agreement in obtaining an appropriate droplet surface obtained by the CLSM over a detail range of surface accuracy (resolution: $2{\mu}m$). And the results allow an excellent accuracy in a measurement of a droplet surface. The combination of extended focal depth measurement configured and multi-scale wavelet filtering has proven that it can construct a droplet surface in a successive and accurate way. A multi-scale approach of wavelet filtering was developed based on the decomposition and reconstruction of droplet surface by 2D wavelet transform using db9 (a mother wavelet of daubechies). Also this technique can be extended to characterize the quantification of droplet properties and other field in a wide range of scales. Finally this method is verified to be a better droplet surface modeling in a micro scale arising in a mist machining.

Flow visualizations and analysis on characteristics of bubbly flows exhausted from a venturi-type bubble generator with an air vent (공기유입구를 가진 벤츄리 형상의 기포발생기에서 토출되는 기포 유동 특성의 가시화 측정 분석)

  • Bae, Hyunwoo;Lee, Seungmin;Song, Moonsoo;Sung, Jaeyong
    • Journal of the Korean Society of Visualization
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    • v.17 no.1
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    • pp.60-68
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    • 2019
  • Flow visualizations have been carried out to analyze the characteristics of bubby flows exhausted from a venturi-type bubble generator with an air vent. For various design parameters and operating conditions of the bubble generator, the images of bubbly flows was recorded using a high-speed camera and a microscope. Then the amount and size distribution of bubble was evaluated by an image processing technique. The results show that for increasing the amount of bubble, it is more effective to reduce the venturi throat than to enlarge the air vent diameter. If the water flow rate increases, the bubble generation rate increases but reaches a status of saturation, whose condition depends on Reynolds number at a given air vent diameter. The bubble size increases as the diameter of venturi throat decreases and Reynolds number increases. However, the air vent diameter is not a significant factor on bubble size.

On the Study of Initializing Extended Depth of Focus Algorithm Parameters (Extended Depth of Focus 알고리듬 파라메타 초기설정에 관한 연구)

  • Yoo, Kyung-Moo;Joo, Hyo-Nam;Kim, Joon-Seek;Park, Duck-Chun;Choi, In-Ho
    • Journal of Broadcast Engineering
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    • v.17 no.4
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    • pp.625-633
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    • 2012
  • Extended Depth of Focus (EDF) algorithms for extracting three-dimensional (3D) information from a set of optical image slices are studied by many researches recently. Due to the limited depth of focus of the microscope, only a small portion of the image slices are in focus. Most of the EDF algorithms try to find the in-focus area to generate a single focused image and a 3D depth image. Inherent to most image processing algorithms, the EDF algorithms need parameters to be properly initialized to perform successfully. In this paper, we select three popular transform-based EDF algorithms which are each based on pyramid, wavelet transform, and complex wavelet transform, and study the performance of the algorithms according to the initialization of its parameters. The parameters we considered consist of the number of levels used in the transform, the selection of the lowest level image, the window size used in high frequency filter, the noise reduction method, etc. Through extended simulation, we find a good relationship between the initialization of the parameters and the properties of both the texture and 3D ground truth images. Typically, we find that a proper initialization of the parameters improve the algorithm performance 3dB ~ 19dB over a default initialization in recovering the 3D information.

THE EFFECT OF GOLD ELECTROFORMING PROCEDURE ON GOLD-SILVER-PALLADIUM ALLOY

  • Hwang, Bo-Yeon;Kim, Chang-Whe;Lim, Young-Jun;Kim, Myung-Joo
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.3
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    • pp.303-309
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    • 2007
  • Statement of problem. The effect of gold electroforming on gold alloy was not studied. Purpose. This in vitro study investigate the effect of gold electroforming on gold-silver-palladium alloy. Material and methods. Three pieces of gold strips had undergone the electroforming procedures on one side and then half of the side again electroformed. The set mode for this study was program 1 ($200{\mu}m$). And the processing time was 15min (1/20 time to form $200{\mu}m$ coping). The confocal laser scanning microscope (PASCAL 5, Carl Zeiss, Bernried, Germany) was used to measure the thickness of the pure gold layer electroformed on the gold strips. Half of the gold strip was coated two times with electroformed gold, and the other half one time. The data from the cone focal laser system was processed to get the vertical profile of the strips and the difference of the vertical height between the double coated and single coated layer was regarded as the thickness of the gold coating. The layer thickness value to built 3D image of the cone-focal laser was set $0.5{\mu}m$. Next to the measurement of the thickness of the coating, the Vicker's hardness test was done. It was performed on the double coated surface, single coated surface and non-coated surface (back side) three times each. Results. The mean thickness value gained from gold electroforming technique was measured to be $22{\mu}m$ for sample 1, $23{\mu}m$ for sample 2, $21{\mu}m$ for sample 3. In the same condition of time, power and the amount of electrolyte, the data showed no difference between samples. According to the results of variance analysis, the differences among the variations in number of coating were statistically insignificant (p>0.05), meaning that the two times of gold electroforming coating did not change the hardness of gold-silver-palladium alloy. Conclusion. The test of thickness of gold coating proved the coherency of the gold electroforming procedure, in other words, when the power, the exposed surface area, processing time and the amount of electrolytes were set same, the same thickness of gold would be coated on. The hardness test showed that the electroformed gold coating did not change the hardness of the gold-silver-palladium alloy when it is coated not more than $45{\mu}m$.

Importance of Impregnation and Polishing for Backscattered Electron Image Analysis for Cementitious Self-Healing Specimen (시멘트계 자기치유 시편에 대한 반사전자현미경 이미지 분석을 위한 함침과 연마의 중요성)

  • Kim, Dong-Hyun;Kang, Kook-Hee;Bae, Seung-Muk;Lim, Young-Jin;Lee, Seung-Heun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.5 no.4
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    • pp.435-441
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    • 2017
  • Studies on self-healing have currently been diversified and the methods to evaluate the studies have become more diversified as well. Among them, the back-scattered electron (BSE) image acquired through the scanning electron microscope (SEM) is attempted as the means to evaluate the self-healing effect on cracks. In order evaluate by the BSE image, sophisticated pre-processing of specimen is critical and this injected inside the particle, pore and artificial crack of the hardener to stabilize the structure of the newly generated self-healing product and it enables to endure the stress on polishing without deformation. The impregnated specimen smoothen the surface to obtain the BSE image of high resolution that polishing is made for diamond suspension for wet polishing after dry polishing. As a result of evaluating the self-healing product on the impregnated and polished self-healing specimen, the generated product is formed from the surface of the artificial crack and the self-healing substances are confirmed as $Ca(OH)_2$ and C-S-H.

Characterization of the Expression of PKCα(Isoform) in DMH-induced Vascular Endothelial Proliferation (DMH에 의한 비정상적인 혈관 내피세포의 증식에서 Protein Kinase C 동종효소 Alpha 단백발현의 특성)

  • Nam, Su Bong;Bae, Yong Chan;Park, Suk Young;Choi, Soo Jong
    • Archives of Plastic Surgery
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    • v.34 no.6
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    • pp.679-684
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    • 2007
  • Purpose: DMH(1,2-dimethylhydrazine) has been known to induce vascular neoplasm such as malignant endothelioma in animal experiment, through induction of abnormal proliferation of HUVECs. In our previous studies, 11 types of PKC isoenzymes were determined by RT-PCR and the expression of $PKC{\alpha}$, and ${\mu}$ was more prominent than other PKC isoenzymes in the DMH-treated group. However, this result was not based on objective assessment. In this study, we further evaluated the role of $PKC{\alpha}$ on the DMH-induced abnormal proliferation of HUVECs by two different methods to identify its presence with high relevance in objective view. $PKC{\mu}$ will be investigated in further study. Methods: The study was conducted with the cultured HUVECs group(control) and the $0.75{\times}10^{-9}M$ DMH-treated group. After processing protein extraction in 0 and 24 hour, extracted protein was treated of quantitative test through BCA protein assay. In the western blot analysis, electrophoresis was performed in the order of gel preparation, sample preparation, and gel running. Electrotransfer to nitrocellulose membrane and reaction with antibody were done. Detection of $PKC{\alpha}$ was achieved through "Gel Image Analysis System". In the fluorescence immunocytochemical analysis, the grading of radiance of the intracellular $PKC{\alpha}$ particles was detected with confocal microscope after treating with primary and fluorescent secondary antibody in 0 and 24 hours. Results: The Western blot analysis showed increased $PKC{\alpha}$ expression from the specimen obtained in 24 hour of the DMH treatment group when compared to those in control group. Under confocal fluorescence microscope, the emitting radiance in the DMH treated group was brighter at 24 hours as well. Conclusion: We believe that $PKC{\alpha}$ plays a role in DMH-induced abnormal proliferation of the vascular endothelium, which may provide insights in understanding the vascular neoplasm.

Sediment monitoring for hydro-abrasive erosion: A field study from Himalayas, India

  • Rai, Anant Kr.;Kumar, Arun
    • International Journal of Fluid Machinery and Systems
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    • v.10 no.2
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    • pp.146-153
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    • 2017
  • Sediment flow through hydropower components causes hydro-abrasive erosion resulting in loss of efficiency, interruptions in power production and downtime for repair/maintenance. Online instruments are required to measure/capture the variations in sediment parameters along with collecting samples manually to analyse in laboratory for verification. In this paper, various sediment parameters viz. size, concentration (TSS), shape and mineral composition relevant to hydro-abrasive erosion were measured and discussed with respect to a hydropower plant in Himalayan region, India. A multi-frequency acoustic instrument was installed at a desilting chamber to continuously monitor particle size distribution (PSD) and TSS entering the turbine during 27 May to 6 August 2015. The sediment parameters viz. TSS, size distribution, mineral composition and shape entering the turbine were also measured and analysed, using manual samples collected twice daily from hydropower plant, in laboratory with instruments based on laser diffraction, dynamic digital image processing, gravimetric method, conductivity, scanning electron microscope, X-ray diffraction and turbidity. The acoustic instrument was able to capture the variation in TSS; however, significant deviations were found between measured mean sediment sizes compared to values found in the laboratory. A good relation was found for turbidity ($R^2=0.86$) and laser diffraction ($R^2=0.93$) with TSS, which indicated that turbidimeter and laser diffraction instrument can be used for continuous monitoring of TSS at the plant. Total sediment load passed through penstock during study period was estimated to be 15,500 ton. This study shall be useful for researchers and hydropower managers in measuring/monitoring sediment for hydro-abrasive erosion study in hydropower plants.

Accuracy of a proposed implant impression technique using abutments and metal framework

  • Lee, Hyeok-Jae;Lim, Young-Jun;Kim, Chang-Whe;Choi, Jung-Han;Kim, Myung-Joo
    • The Journal of Advanced Prosthodontics
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    • v.2 no.1
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    • pp.25-31
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    • 2010
  • PURPOSE. This study compared the accuracy of an abutment-framework (A-F) taken with open tray impression technique combining cement-on crown abutments, a metal framework and resin cement to closed tray and resin-splinted open tray impression techniques for the 3-implant definitive casts. The effect of angulation on the accuracy of these 3 techniques was also evaluated. MATERIAL AND METHODS. Three definitive casts, each with 3 linearly positioned implant analogs at relative angulations 0, 30, and 40 degrees, were fabricated with passively fitted corresponding reference frameworks. Ten impressions were made and poured, using each of the 3 techniques on each of the 3 definitive casts. To record the vertical gap between reference frameworks and analogs in duplicate casts, a light microscope with image processing was used. Data were analyzed by two-way analysis of variance and the Tukey test. RESULTS. The open tray techniques showed significantly smaller vertical gaps compare to closed tray technique (P < .05). The closed tray and the resin-splinted open tray technique showed significantly different vertical gaps according to the angulation of implant (P < .05), but the A-F impression technique did not (P > .05). CONCLUSION. The accuracy of the A-F impression technique was superior to that of conventional techniques, and was not affected by the angulation of the implants.

The Reconstructive Method for The Enhancement of Depth Resolution for Acoustic Image using the Spatial Frequency Response in NPPs' Material (NPP 매질내에서 공간주파수 응답을 이용한 초음파 영상의 깊이 분해능 개선을 위한 복원 방법)

  • Koo, Gil-mo;Kim, Hyun;Park, In-ho
    • Journal of Korea Multimedia Society
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    • v.5 no.4
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    • pp.426-433
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    • 2002
  • In this paper, we have studied the images which have been reconstructed by using combination of images acquired by the variation of operating frequency. When inner images have been reconstructed, they have been superposed by the surface state effect. In this case, the images of the phase object can be enhanced by the contrast of inner images. In this experiment, there are two kinds of specimens, one is a reference block haying 1/4T, 1/2T, 3/4T side drilled holes as main run piping material 111 the steam generator in NPP(Neuclear Power Plant)s and the another is a part of a hemisphere type specimen having about 1-2㎜ distance gap. It has been shown that the two results of defect shapes have better than before in this processing and phase contrast grow about twice. And we have constructed the acoustic microscope by using a quadrature detector that enables to acquire the amplitude and phase of the reflected signal simultaneously Further more ore have studied the reconstruction method of the amplitude and phase images and the enhancement method of the defect images contrast.

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