• Title/Summary/Keyword: microscope image processing

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Deep Learning-based Automatic Wrinkles Segmentation on Microscope Skin Images for Skin Diagnosis (피부진단을 위한 딥러닝 기반 피부 영상에서의 자동 주름 추출)

  • Choi, Hyeon-yeong;Ko, Jae-pil
    • Journal of Advanced Navigation Technology
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    • v.24 no.2
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    • pp.148-154
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    • 2020
  • Wrinkles are one of the main features of skin aging. Conventional image processing-based wrinkle detection is difficult to effectively cope with various skin images. In particular, Wrinkle extraction performance is significantly decreased when the wrinkles are not strong and similar to the surrounding skin. In this paper, deep learning is applied to extract wrinkles from microscopic skin images. In general, the microscope image is equipped with a wide-angle lens, so the brightness at the boundary area of the image is dark. In this paper, to solve this problem, the brightness of the skin image is estimated and corrected. In addition, We apply the structure of semantic segmentation network suitable for wrinkle extraction. The proposed method obtained an accuracy of 99.6% in test experiments on skin images collected in our laboratory.

Observation of the Domain Structures in Soft Magnetic (Fe97A13)85N15/Al2O3 Multilayers

  • Stobiecki, T.;Zoladz, M.
    • Journal of Magnetics
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    • v.8 no.1
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    • pp.13-17
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    • 2003
  • The longitudinal magnetooptical Kerr effect was used to analyse magnetic domains in soft magnetic ${(Fe_{97}A1_3)}_{85}N_{15}$/$Al_{2}O_{3}$ multilayers in order to get microscopic understanding of interlayer exchange coupling. The measuring system consists of a Kerr microscope, a CCIR camera (with an 8-bit framegrabber), 16 bit digital camera and computer system for real-time image processing and to control external magnetic field and cameras. The strength of ferromagnetic (EM) coupling as a function of the spacer thickness of $Al_2O_3$ was investigated. It was found that strong FM-coupling, strong uniaxial anisotropy and coherent rotation of the magnetization have been observed for the spacer thickness in the range of 0.2 nm $\leq$ t $\leq$ 1 m, however, weak FM-coupling, patch domains and $360^{\circ}$-walls occur for the spacer thickness of t = 2.5 nm. At a spacer thickness of t $\geq$ 5 nm transition takes place from weak FM-coupling to the decoupled state where complex interlayer interactions and different types of the domain walls were observed.

Evaluation of soil spatial variability by micro-structure simulation

  • Fei, Suozhu;Tan, Xiaohui;Wang, Xue;Du, Linfeng;Sun, Zhihao
    • Geomechanics and Engineering
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    • v.17 no.6
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    • pp.565-572
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    • 2019
  • Spatial variability is an inherent characteristic of soil, and auto-correlation length (ACL) is a very important parameter in the reliability or probabilistic analyses of geotechnical engineering that consider the spatial variability of soils. Current methods for estimating the ACL need a large amount of laboratory or in-situ experiments, which is a great obstacle to the application of random field theory to geotechnical reliability analysis and design. To estimate the ACL reasonably and efficiently, we propose a micro-structure based numerical simulation method. The quartet structure generation set algorithm is used to generate stochastic numerical micro-structure of soils, and scanning electron microscope test of soil samples combined with digital image processing technique is adopted to obtain parameters needed in the QSGS algorithm. Then, 2-point correlation function is adopted to calculate the ACL based on the generated numerical micro-structure of soils. Results of a case study shows that the ACL can be estimated efficiently using the proposed method. Sensitivity analysis demonstrates that the ACL will become stable with the increase of mesh density and model size. A model size of $300{\times}300$ with a grid size of $1{\times}1$ is suitable for the calculation of the ACL of clayey soils.

Study on Highly Accuracy Quality Evaluation of Spot Weld by use of Image Processing Technique

    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.5 no.4
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    • pp.38-46
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    • 1996
  • This paper discusses the feasibility of Ultrasonic Nondestructive Evaluation (UNDE) technique for sport weld quality. Ultrasonic c-scan image assisted by image processing technique was used for Nondestructive Evaluation(NDE) of spot weld quality. Ultrasonic testing results obtained were confirmed and compared by Optical Microscope and SAM(Scanning Acoustic Mircroscope) observation of the spot-weld cross section, The results show that the nugget dinameter can be successfully measured with the accuracy of 0.5mm. It was ascertained that ultrasonic c-scan technique is very effective method for the sake of the approach to the quantitative measurement of nugget diameter and the discrimination of the corona bond from nugget. Additional support for the above conclusions is provided by the results for galvanized steel. The ultrasonic results for galvanized welds generally correspond to the results for uncoated steel. Finally, it was found that the above-mentioned technique can be sufficiently applied to NDE method for securing the Quality Assurance(QA) of spot welded products in production line.

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Quantification of Melanin Density at Epidermal Basal Layer by Using Confocal Scanning Laser Microscope (CSLM) (Confocal Scanning Laser Microscope (CSLM)을 이용한 피부 기저층 멜라닌 밀도의 정량화)

  • Kim, Dong Hyun;Lee, Sung Ho;Oh, Myoung Jin;Choi, Go Woon;Yang, Woo Chul;Park, Chang Seo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.3
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    • pp.259-268
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    • 2014
  • Non-invasive technologies in skin research have enabled to use a live image of living skin without a biopsy or histologic processing of tissue. Confocal scanning laser microscope (CSLM) operated at a near-infrared wavelength of 830 nm allows visualization of inner structure of skin as a non-invasive manner. According to previous researches using CSLM, melanin cap and papillary ring were clearly observed in pigmented areas between stratum basale and papillary dermis. In this study, conversional analysis of CSLM digital images into numerical estimation using scanning probe image processor (SPIP) software was attempted for the first time. It is concluded that a quantification of CSLM images can pave way to expand the field of applications of CSLM.

Image Analysis Algorithm for the Corneal Endothelium

  • Kim Young-Yoon;Kim Beop-Min;Park Hwa-Joon;Im Kang-Bin;Lee Jin-Su;Kim Dong-Youn
    • Journal of Biomedical Engineering Research
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    • v.27 no.3
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    • pp.125-130
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    • 2006
  • The number of the living endothelial cells and the shape of those are very import clinical parameters for the evaluation of the quality of cornea. In this paper, we developed the automated endothelial cell counting and shape analysis algorithm for a confocal microscope. Since, the endothelial images from the confocal microscope has a non-uniform illumination and low contrast between cell boundaries and cell bodies, it is very difficult to segment the cells from the endothelial images. To cope with these difficulties, we proposed the new two stage image processing algorithm. At first stage algorithm, we used a high-pass filter and histogram equalization to compensate the non-uniform brightness pattern and a morphological filter and a watershed method are applied to detect the boundary of cells. From this stage, we could count the number of cells in an endothelial image. At second stage algorithm, we used a Voronoi diagram method to classify the shape of cells. This cell shape analysis and the percent of hexagonal cells are very sensitive in detecting the early endothelium damage. To evaluate the performance of the proposed system, we p개cessed seven endothelial images obtained using a confocal microscope. The proposed system correctly counted 95.5% cells and classified 92.0% of hexagonal cell shapes. This result is better than any others in this research area.

Development of Electron Beam Monte Carlo Simulation and Analysis of SEM Imaging Characteristics (전자빔 몬테 카를로 시물레이션 프로그램 개발 및 전자현미경 이미징 특성 분석)

  • Kim, Heung-Bae
    • Journal of the Korean Society for Precision Engineering
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    • v.29 no.5
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    • pp.554-562
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    • 2012
  • Processing of Scanning electron microscope imaging has been analyzed in both secondary electron (SE) imaging and backscattered electron (BSE) image. Because of unique characteristics of both secondary electron and backscattered electron image, mechanism of imaging process and image quality are quite different each other. For the sake of characterize imaging process, Monte Carlo simulation code have been developed. It simulates electron penetration and depth profile in certain material. In addition, secondary electron and backscattered electron generation process as well as their spatial distribution and energy characteristics can be simulated. Geometries that has fundamental feature have been imaged using the developed Monte Carlo code. Two, SE and BSE images generation process will be discussed. BSE imaging process can be readily used to discriminate in both material and geometry by simply changing position and direction of BSE detector. The developed MC code could be useful to design BSE detector and their position. Furthermore, surface reconstruction technique is possibly developed at the further research efforts. Basics of Monte Carlo simulation method will be discussed as well as characteristics of SE and BSE images.

Application of Three-Dimensional Light Microscopy for Thick Specimen Studies

  • Rhyu, Yeon Seung;Lee, Se Jeong;Kim, Dong Heui;Uhm, Chang-Sub
    • Applied Microscopy
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    • v.46 no.2
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    • pp.93-99
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    • 2016
  • The thickness of specimen is an important factor in microscopic researches. Thicker specimen contains more information, but it is difficult to obtain well focused image with precise details due to optical limit of conventional microscope. Recently, a microscope unit that combines improved illumination system, which allows real time three-dimensional (3D) image and automatic z-stack merging software. In this research, we evaluated the usefulness of this unit in observing thick samples; Golgi stained nervous tissue and ground prepared bone, tooth, and non-transparent small sample; zebra fish teeth. Well focused image in thick samples was obtained by processing z-stack images with Panfocal software. A clear feature of neuronal dendrite branching pattern could be taken. 3D features were clearly observed by oblique illumination. Furthermore, 3D array and shape of zebra fish teeth was clearly distinguished. A novel combination of two channel oblique illumination and z-stack imaging process increased depth of field and optimized contrast, which has a potential to be further applied in the field of neuroscience, hard tissue biology, and analysis of small organic structures such as ear ossicles and zebra fish teeth.

Measurement of Size Distributions of Submicron Electrosprays Using a Freezing Method and an Image Processing Technique (냉각법 및 영상 처리기법을 이용한 서브마이크론 정전분무 액적의 크기분포 측정)

  • Gu, Bon-Gi;Kim, Sang-Su;Kim, Yu-Dong;Lee, Sang-Yong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.25 no.10
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    • pp.1400-1407
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    • 2001
  • The size distributions of electrospray droplets from the Taylor cone in cone-jet mode are directly measured by using a freezing method and a transmission electron microscope (TEM) image processing technique. These results are compared with the data obtained by an aerodynamic size spectrometer (TSI Aerosizer DSP). The use of glycerol seeded with NaI and a freezing method make it possible to sample droplets with their original sizes preserved. Since pictures of droplets are taken with TEM with very low vapor pressure of the solution, evaporation is suppressed by freezing. For liquid flow rates below 1 nl/sec, the measured droplet diameters by the TEM image processing technique and the aerosizer are in the range of 0.25 to 0.32 m add 0.3B to 0.40m, respectively. Comparing the TEM data with the aerosizer measurements, it has been revealed that the TEM image processing technique can afford more accurate values of droplet size distributions in the submicron range of 0.1 to 0.4m.

Visual Recognition of Magnetc Domain Pattern Using Pixel Value Operation (픽셀값 연산을 이용한 자성체의 자구패턴 시각화)

  • Kim, Young-Hak
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.681-684
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    • 2015
  • Magnetization is very important in the ferro-magnetic physics and provides useful informations in the application field of magnetic devices. Generally, the only first acquired domain pattern is not helpful to recognize domain pattern. Many images are needed to visualize domain pattern through image processing. These images were obtained a 8-bit digital camera. The operation was the subtraction of pixel values of multi domain imanges from the images with 255 of pixel value, which was obtained in the saturated state of magnetic materials. The magnetic domain images was visualized gradually with increasing the number of subtracion operation. LABVIEW was used as an image processing tool and the optic microscope with a polarizer was used in this experiment.

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