• 제목/요약/키워드: micropatterning

검색결과 39건 처리시간 0.028초

N-hydroxysuccinimidyl phenyl azide와 광반응을 이용한 펩타이드의 마이크로형태 고정화 (Micropatterning of Peptides to Solid Surface by Deep-UV Lithography using N-hydroxysuccinimidyl phenol azide)

  • 김진희;김현정;김종원;장준근;민병구;최태부
    • 대한의용생체공학회:의공학회지
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    • 제19권5호
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    • pp.441-448
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    • 1998
  • 폴리머 표면에 생체물질의 고정화 방법은 생체적합성을 자닌 재료의 개발에있어서 중요한 방법중의 하나이다. 광반응을 이용한 photolithography방법을 사용하여 재료 표면의 원하는 부위에 단백질을 고정할 수 있다. 본 연구에서는 파이브로넥틴의 세포부탁 리간드, GRGDS펩타이드를 N-hydroxysuccinimidyl phenol azide를 이용하여 미세한 선의 형태로 표면에 광반응으로써 고정하였다. 광반응 유도체가 고정된 표면은 형광물질을 사용하여 확인하였다. 또한 혈관내피 세포는 GRGDS펩타이드가 고정화된 표면에서만 부탁됨을 관찰하였다.

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Polyelectrolyte Micropatterning Using Agarose Plane Stamp and a Substrate Having Microscale Features on Its Surface

  • Lee, Min-Jung;Lee, Nae-Yoon;Lee, Sang-Kil;Park, Sung-Su;Kim, Youn-Sang
    • Bulletin of the Korean Chemical Society
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    • 제26권10호
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    • pp.1539-1542
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    • 2005
  • We have introduced polyelectrolyte micro-patterning technique employing agarose plane stamp and a hard substrate having microscale features on its surface. With this method, chemically micropatterned surfaces with both positive and negative functionalities were successfully embedded in well-defined microstructures, and selective impartment of charge functionalities was confirmed by patterning bead bearing surface charge. Furthermore, this technique allows highly sensitive immobilization of protein onto targeted surface simply by endowing functionalities, which extends the potential of its use as a tool for high-throughput protein microarray and proteomics. Because plane agarose stamp is free of structures on its surface, there is no concern for pattern collapse, and the combination of agarose plane stamp with patterned substrate is more suited for selective protein patterning compared with adopting surface-patterned agarose stamp with flat substrate. Our technique using agarose plane stamp and a substrate having microscale features on its surface suggests a range of possible applications, including the micropatterning of biofunctionalized copolymer having polyelectrolyte block, immobilization of micro- and nanoparticle with biofunctionalities such as biotin and streptavidine, and establishing optoelectronic microstructures with micro-beads on various surfaces.

Fluorescence Micropatterning Based on the Polymeric Photobase Generator Containing Oxime-Urethane Groups by Dansylation

  • Choi, Won San;Kim, Hak Soo;Chae, Kyu Ho
    • Rapid Communication in Photoscience
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    • 제2권1호
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    • pp.24-27
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    • 2013
  • A polymeric photobase generator containing oxime-urethane groups is applied to a fluorescence micropatterning material. Polymer bearing oxime-urethane groups was prepared by copolymerization of methyl methacrylate with methacryloyloxyethyl benzophenoneoxime urethane (MBU). The reaction of amino groups in the irradiated copolymer film with dansyl chloride (Dns-Cl) was monitored by using UV absorption, IR absorption and fluorescence spectroscopy. The fluorescence spectrum of the Dns-Cl-treated irradiated copolymer film shows a strong fluorescence with a fluorescence maximum wavelength at 510 nm. A blue fluorescent micropattern with a line width of $2{\sim}3{\mu}m$ was obtained. Treatment of the irradiated copolymer film with Dns-Cl and rhodamine B mixture led to the formation of green, red, and orange-colored fluorescence micropatterns. Thus, various colored micropatterns on a single polymer film can be obtained by selective excitation of each dye molecules.

고정 마스크에 의한 레이저 미세패터닝 쾌속 제작 (Rapid Manufacturing of Laser Micro-Patterning Using Fixed Masks)

  • 신보성;오재용
    • 한국레이저가공학회지
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    • 제9권1호
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    • pp.17-23
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    • 2006
  • The technologies of laser micromachining are changed toward more complex-micropatterning, from the micro circle-shaped hole drilling to the micro arbitrary-shaped hole drilling. In this paper, the fundamental experiments by using DPSS 3rd harmonic $Nd:YVO_4\;laser({\lambda}=355nm)$ were carried out in order to obtain the feasibility of flexible micropatterning by various fixed masks. Fixed masks and Galvano scanners were investigatde to make micro patterns. from these experimental results, micropatterns on PEN film were rapidly manufactured in large area.

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Laser Ablation : Fundamentals and applications in Micropatterning and Thin Film Formation

  • J. Heitz;D. Bauerle;E. Arenholz;N. Arnold;J.T. Dickinson
    • Journal of Photoscience
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    • 제6권3호
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    • pp.103-108
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    • 1999
  • We present recent results on ablation mechanism, single-pulse laser micropatterning , pulsed-laser deposition(PLD) and particulates formation accompanying laser ablation, with special emplasis on polymers, in particular polymide, (PI), and polytetrafluoroethylene, (PTFE). Ablation of polymers is described on the basis of photothermal bond breaking within the bulk material. Here, we assume a first order chemical reaction, which can be described by an Arrhenius law. Ablation starts when the density of broken bonds at the surface reaches a certain critical value. Single-pulse laser ablation of polyimide shows a clear-length dependence of the threshold fluence. This experimental result strongly supports a thermal ablation model. We discuss the various possibilities and drawbacks of PLD and describe the morphology, physical properties and applications of PTFE films.

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