• Title/Summary/Keyword: microorganism growth

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Effect of Microbial Product on Microorganisms in Soil and the Growth of Leaf Lettuce (EM 활성액, 키토산 및 목초액 처리가 토양 미생물상의 변화 및 잎 상추의 생육에 미치는 영향)

  • Seok, Woon-Young;Oh, Ju-Sung;Kim, Doh-Hoon;Chung, Won-Bok;Jeong, Soon-Jae
    • Korean Journal of Organic Agriculture
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    • v.12 no.4
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    • pp.427-436
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    • 2004
  • This study was conducted to investigate the effect of microbial product on microorganisms in soil and the growth of leaf lettuce. The test material were treated with chitosan, wood vinegar and EM activity liquid, and treatment concentration was 50 times solution and 100 times solution level with foliar application. The results were summarized as follows : Among foliar application of microorganisms treatments diluted by chitosan 100 times solution level was effective considering growth of leaf lettuce as compared other dilutions and control plot. Change of microorganism number in the soil for cultivation of chinese cabbages and leaf lettuce was increased with microorganism treatment plot as compared with control plot. Specially chitosan 100 times solution level showes the most significant effect.

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Effect of Soft Ferrite Ceramic Powders on the Growth and Viability at Low Temperature of Antagonistic Microorganisms (연자성 세라믹 분말이 길항미생물의 생장과 저온 활성에 미치는 영향)

  • Sun, Heung-Suk;Park, Chan-Young
    • Journal of the Korean Society of Industry Convergence
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    • v.2 no.1
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    • pp.85-90
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    • 1999
  • The possibility of employment of ceramics, known to be emitting far infrared waves, to keep the growth and the viability of the antagonistic microorganisms was examined. Among four kinds of commercial ceramics, the ceramic powders composed of soft ferrite as a main component has exhibited to grow Pseudomonas cepacia and Saccharomyces cerevisiae better. The ceramic powder in the growth medium has increased the number of cells of P. cepada about ten times and that of S. cerevisiae two to five times more than that in the control at $27^{\circ}C$. The viability of the microorganism at low temperature which was measured from the regrowth behavior at $27^{\circ}C$ after five days store at $4^{\circ}C$ has shown that the lag time of the two microorganisms reduced about three hours without any defect in the rate of logarithmic growth. These results demonstrated that the ceramic powders was available to the growth and viability at low temperature of antagonistic microorganisms.

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The Effect of Aeration Rate on the Production of Exopolysaccharide, EPS-R by Marine Microorganism 96CJ10356

  • Lee, Hyeon-Sang;Park, Sin-Hye;Lee, Jong-Ho;Lee, Hong-Geum
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.211-214
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    • 2000
  • Marine Microorganism strain 96CJ10356 produced extracellular polysaccharide (EPS-R) accompanied with cell growth. To improve the production of EPS-R, the effect of aeration rate was tested in a 5-liter jar fermentor with STN medium. The production of EPS-R was increased with aeration rate and after 72 hour cultivation, 12.20 g/l of EPS-R was obtained with an aeration rate of 1.5 vvm and the apparent viscosity was measured to be about 1000 cp with culture broth.

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Biodegradable Characteristics of Starch-filled Polyethylene Film by Fungi (전분이 함유된 폴리에틸렌 필름의 곰팡이에 의한 생분해 특성)

  • 김재현;박태현
    • KSBB Journal
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    • v.9 no.4
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    • pp.412-417
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    • 1994
  • The biodegradable characteristics of starch-filled polyethylene film by fungi were investigated. Aspergillus niger showed the highest cell growth among five species of fungi used in the experiment. Higher starch content gave the higher growth rate of A. niger; however, the kind of film or cutting direction did not give any effects on the cell growth. The use of vinyl trimethoxysilane for the stronger binding of starch and polyethylene did not inhibit the cell growth. When the starch content was higher than 10%, the elongation of the film decreased significantly after the growth of microorganism in the case of transverse direction samples.

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Comparison of Mycelial Growth Characteristics According to Culture Conditions of Ulleungdo Collection Strains (울릉도 수집 균주의 배양조건에 따른 균사 생장 특성 비교)

  • Kim, Minkyeong;Ahn, Chorong;Kim, Changmu
    • The Korean Journal of Mycology
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    • v.48 no.2
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    • pp.75-85
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    • 2020
  • The collection of biological data of indigenous species must comply with the Nagoya Protocol. Fungi contain various bioactive substances making them an attractive source of several products, including food and medicines. In this study, we investigated the growth characteristics of five indigenous fungal strains, Fomitiporia punctata, Polyporus ulleungus, P. brumalis, Gymnopus subnudus, and Tyromyces kmetii, isolated from samples collected in the Ulleungdo Island. The growth rates for each strain were assessed across various temperatures (20 ℃ to 35 ℃), culture media (Potato dextrose agar, Malt extract & Yeast extract agar, Malt extract agar, Malt extract & peptone agar, Sabouraud dextrose agar, and Modified Melin-Norkrans agar), and pH conditions (4.0 to 8.0). Additionally, we assessed the mycelial growth characteristics in liquid culture. The mycelial growth in different media varied across species; specifically, F. punctata (in MMNA), G subnudus (in MMNA), and P. brumalis (in MEPA) showed rapid growth. Optimal growth temperatures ranged between 25 ℃ and 30 ℃ for most species, with the exception of T. kmetii and P. brumalis, which were able to grow across all the temperatures tested. P. brumalis showed the best growth rate, whereas P. ulleungus showed the lowest growth potential. The optimal pH conditions for mycelial growth ranged between 4.0 and 5.0. In experiments using culture flasks, the dry weight of the culture filtrates decreased with the increasing incubation time and showed a significant decrease between 1 and 6 months of incubation, indicating that the five strains take longer than a month to fully use the culture media. Our findings highlight and establish the optimal growth conditions for five different fungal species that can be used in future application studies.

Enzymological Evaluation of Bowel Inflammation Inhibitory Activity and Intestinal Microbial Flora Improvement by Enzymatic Hydrolysate of Schizandrae Fructus (오미자 효소가수분해물에 의한 장내 세균총 개선 및 장관 염증 억제 효과의 효소학적 평가)

  • Ryu, Il-Hwan;Kwon, Tae-Oh;Lee, Kang-Soo;Yun, Yong-Gab
    • Korean Journal of Pharmacognosy
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    • v.38 no.4
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    • pp.363-371
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    • 2007
  • Schizandrae Fruits has been used as a traditional Oriental medicine for treatment of many stress-induced diseases. In the present study, we investigated inhibitory activity of enzymatic hydrolysate of Schizandrae fructus (SC-EX) in growth of tested intestinal microorganism and activity of bowel inflammation related enzyme. SC-EX was added to the proteose peptone-yeast extract-fildes (PYF) media to investigation the effect on the growth of type culture of intestinal microorganism. The growth of lactic acid bacteria such as Bifidobacterium species and Lactobacillus species was accelerated by more than 3% concentration of SC-EX. But, growth of harmfulness bacteria such as E.coli, Clostridium sp. Staphylococcus sp. Streptococcus sp. was inhibited by more than 3% concentration of SC-EX. Also, SC-EX was exhibited a concentration-dependent inhibitory activity of the bowel inflammation related enzymes. The SC-EX was showed 76% and 92% inhibitory activity of 5-lipoxygenase and cyclooygenase at 5% additional concentration respectively. Our results indicated that SC-EX may possess improvement effect on the intestinal flora and Anti-inflammatory effect on the bowel.

Evaluation of Optimum Conditions for Bacteriocin Production from Lactobacillus sp. JB-42 Isolated from Kimchi

  • Jo, Young Bae;Kyung Mi Bae;Sung Koo Kim;Hong Ki Jun
    • Journal of Microbiology and Biotechnology
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    • v.6 no.1
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    • pp.63-67
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    • 1996
  • Bacteriocin-producing microorganism was isolated from Kimchi. The microorganism was identified as a Lactobacillus sp. and named Lactobacillus sp. JB-42. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For the maximum yield of bacteriocin from Lactobacillus sp. JB-42, the cell should be harvested at the early stationary phase and the temperature, pH and NaCl concentration should be $30^{\circ}C$, pH 7 and without the addition of NaCl, respectively. Sucrose, glucose or fructose should be used as a carbon source and organic nitrogen such as tryptone should be used as a nitrogen source for the best yield. The production of bacteriocin was related to the cell growth of Lactobacillus sp. JB-42 indicating the role of Lactobacillus in the Kimchi fermentation process.

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THE CONTACT LENS RELATED CASE REPORTS IN PEDIATRIC POPULATION I -Congenital Cataract With Divergent Squint (콘택트렌즈에 연관된 소아집단 I 에서 증례 보고 -개산성 사시를 가지는 선천성 백내장)

  • Chen, Ai-Hong;Kim, Douk Hoon
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.1
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    • pp.75-79
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    • 2004
  • Some ocular complications were connected to the contamination by the contact lens related. Especially the most cause of ocular complications was a microorganism from several processing of infections. Therefore the patients stopped the contact lens wear immediately by the side effects of ocular pathology. We had analyzed the microorganism from the laboratory. It was identified as mixed growth of the Chryseamonas meningosepticum and Enterobacter sp.

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Extraction and Degradation of Alginate from Brown Seaweed Stem Using Microorganism (미생물을 이용한 미역줄기에서 알긴산염 추출 및 저분자화)

  • Ahn Seong-Jun;Kim Young-Sook;Park Kwon-Pil
    • Journal of Environmental Science International
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    • v.13 no.12
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    • pp.1139-1144
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    • 2004
  • We studied a extraction and degradation of alginate from seaweed-stems using microorganism DS-02. DS-02 has a maximum growth rate at $30^{\circ}C$ and the enzyme has a maximum activity of alginate extraction at $35^{\circ}C.$ The yield of alginate extraction using DS-02 is about $16.0{\%}$ for 3.0 hour and molecular weight of the alginate decreased to about 1/8 of initial value after 24 hour extraction. Alginate extraction method by DS-02, compared with general alkali-extraction method, has an advantage of decreasing the molecular weight of alginate during extraction.

In vitro effects of titanium on oral microorganism (In vitro에서 titanium이 구강미생물에 미치는 영향)

  • Lee, H.S.;Bae, B.J.;Kim, J.
    • Journal of Technologic Dentistry
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    • v.21 no.1
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    • pp.145-151
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    • 1999
  • Titanium(Ti) alloys has been mostly concerned with biocompatibility, corrosion resistance, and biofunctionality. However, very little is known, about the biological effects of titanium on microorganism and in particular on the oral flora. So, the effect of titanium on the in vitro growth of oral microorganism forming dental caries was studied under either aerobic or anaerobic condition. In this study, the mostly bacterial species commonly found in dental plaque or gingival sulcus grew well in an aqueous medium containing $100{\mu}g/ml$ of titanium standard solution.

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