• Title/Summary/Keyword: microbiological test

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Clinical and microbiological effects of the supplementary use of an erythritol powder air-polishing device in non-surgical periodontal therapy: a randomized clinical trial

  • Park, Eon-Jeong;Kwon, Eun-Young;Kim, Hyun-Joo;Lee, Ju-Youn;Choi, Jeomil;Joo, Ji-Young
    • Journal of Periodontal and Implant Science
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    • v.48 no.5
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    • pp.295-304
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    • 2018
  • Purpose: This study was undertaken to evaluate the clinical and microbiological effects of an erythritol powder air-polishing device (EPAP) as a supplement to scaling and root planing (SRP) therapy in patients with moderate chronic periodontitis. Methods: Clinical and microbiological evaluations were performed at 21 sites treated with SRP (control) and 21 sites treated with SRP+EPAP (test). All examinations were performed before treatment, 1 month after treatment, and 3 months after treatment. Results: There were no significant clinical differences between the test group and the control group. Microbiological analysis revealed that the relative expression level of Porphyromonas gingivalis was significantly lower in the test group than in the control group at 1 month after treatment. Clinical and microbiological results showed improvements at 1 month compared to baseline; in contrast, the results at 3 months after treatment were worse than those at 1 month after treatment. Conclusions: In this study, both SRP and SRP+EPAP were clinically and microbiologically effective as non-surgical periodontal treatments. In particular, the SRP+EPAP group showed an antimicrobial effect on P. gingivalis, a keystone bacterium associated with the onset of chronic periodontitis, in a short-term period. Periodic periodontal therapy, at intervals of at least every 3 months, is important for sustaining the microbiological effects of this treatment.

The Microbiological and Sensorial Properties of frozen bibimbap namul during storage (저장기간에 따른 냉동 비빔밥 나물의 미생물학적, 관능적 특성)

  • 한영실;박지영
    • Korean journal of food and cookery science
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    • v.17 no.2
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    • pp.149-155
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    • 2001
  • Namul cooked with the standard recipe was examined by research of microbiological test for three months and sensory evaluation of frozen namul after reheating. When the namul was freezed storage, in the microbiological test namul began to change on the 40th day, but there was no problem about stability of storage until 3 months. The overall qualities of taste, flavor, color and texture were examined by sensory evaluation of frozen namul after reheating. The pH was seemed to change slowly, its color was changed on the 20th day from the beginning of storage. Radish root represented substantial difference in texture and overall quality on the 20th day. The off-flavor of immature pumpkin stated on the 25th day. Later 10days nettle tree mushroom began to be changed in its taste, texture, overall quality and appearance, then on the 25th day it was seemed to have low preference. But oak mushroom kept its quality good for 25 days. Oyster mushroom was changed in color, appearance and overall quality on the 20th day. Bracken had low preference in taste, texture and moisture on the 25th day. The color of spinach was changed on the 15th day, and its taste on the 20th day. Soybean sprout was changed in taste, texture and overall quality on the 15th day, and overall quality marked low preference on the 25th day Root of bell flower was changed on the 25th day(p<0.05).

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Effects of Cosmetic Pigments on the Bactericidal Activities of Parabens (파라벤류의 방부력에 대한 화장품용 안료의 영향)

  • Cho, Wan-Goo;Lee, Young-Hwa;Hwang, Seong-Jin
    • Journal of the Korean Applied Science and Technology
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    • v.27 no.4
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    • pp.501-507
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    • 2010
  • In this study, we evaluate the anti-microbiological activity of paraben in eye shadows that are composed of pigments and oil binders using various analytical methods and microbiological tests. Paraben does not show the microbiological activity properly when it was used with Nylon SP$^{(R)}$ 10, Talc RF SSA$^{(R)}$, OMC Talc AS$^{(R)}$ and $BaSO_4$. In the test of fungi, Nylon SP$^{(R)}$ 10 causes the decrease of microbiological activity regardless of the type of oil binders. The pigment of Mango violet also causes the decrease of microbiological activity when ester oil binder was used. Regardless of the type of oil binder, samples containing nylon SP 10, 0.15% of methyl paraben and 0.05% of propyl paraben had not been able to maintain microbiological activity only if the concentration of parabens were increased. Trace amounts of metal ions present in pigments reduced the activity of preservatives by inactivation of hydroxyl group of paraben. It is thought that swollen nylon SP 10 in ester oil increase the absorption or interaction of parabens and swollen nylon powder causes the inactivation of paraben.

Changes in Physicochemical Properties of Packaged Backsulgi during Storage (포장한 백설기의 저장과정 중 이화학적 변화)

  • 이경아;김경자
    • Korean Journal of Human Ecology
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    • v.5 no.2
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    • pp.45-54
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    • 2002
  • Packaged Backsulgi cooked by steam/convection oven and then rapidly chilled was examined by research of microbiological test and sensory evaluation while storing them at the temperatures of 3$^{\circ}C$ and 3$0^{\circ}C$ for 0, 2, 4, 6, 10 days . The pH and reducing sugar content were seemed to change little at 3$^{\circ}C$. However the pH was rapidly reduced until 4 days and then decreased a little at 3$0^{\circ}C$, the reducing sugar content was inclosed little by little. In the microbiological test, any microbial growth in total aerobic, psychrophilic, anaerobic, spore forming bacteria, yeast and molds was not observed until 10 days at 3$^{\circ}C$, but microbial changes of aerobic, psychrophilic and anaerobic bacteria increased to 6 logCFU/g until 10 days at 3$0^{\circ}C$. However microbial changes of them decreased from 6 logCFU/g to 5 logCFU/g. As a result of the sensory evaluation, appearance, taste, color, softness, chewiness and overall Quality were significantly decreased during storage times(p<0.05), but scores of taste and overall quality on 6th days were 7.38${\pm}$1.06, 7.00${\pm}$0.93. Therefore we concluded that there was no problem about stability of storage 6 days at 3$^{\circ}C$.

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Hazard Analysis of Commissary School Foodservice Operations (공동조리 학교급식의 미생물적 품질보증을 위한 위험요인 분석)

  • 곽동경;남순란;김정리;박신정;서소영;김성희;최은희
    • Korean journal of food and cookery science
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    • v.11 no.3
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    • pp.249-260
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    • 1995
  • 6 Central commissary and 2 conventional school foodservice operations were assessed in terms of time-temperature relationship and microbiological quality, and monitoring control methods were identified through hazard analysis during the phases of prodution and distribution. 2 conventional schools from Seoul and 6 commissary schools from Kyungkido were participated in the survey. Meals produced in central commissary were distributed to satellites, therefore delivery practices of foods were identified as critical. Microbiological test results for commissary and conventional schools revealed that microbiological quality of foods was mainly related to time-temperature management, types of food, and equipment sanitation not to the foodservice system used. Time-temperature profiles at temperature danger zone (7.2-60$^{\circ}C$) observed were to be related to the following sanitary practices: cooked vegetables were held at temperature danger zone for relatively longer delayed time (15-38$^{\circ}C$: 15-226 min, 7-60$^{\circ}C$: 75-226 min), and same results were observed for deep-fat fried cutlets (15-38$^{\circ}C$: 15-151 min, 7-60$^{\circ}C$: 33-151 min). Menu items with various ingredients and frequent contacts with hands and equipments during the production flow were held at temperature danger zone for longer delaying time than other menu items with brief prodution stages. Based on hazard analysis critical control points, microbiological quality was collectively affected by time-temperature relationships, equipment sanitation, proper cooking methods, and sanitary management competencies of dietitians. Microbiological test results of working equipments and surface of dishes and trays showed that immediate action should be taken. Cutting boards used in central kitchen were also showed similar results of potential dager of cross-contamination. Effective sanitary control methods were urgently needed.

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The comparison of microbiological control level between HACCP-accredited ice cream manufacturers and non-accredited ones in the southern part of Gyeonggi province, Korea (경기 남부지역 HACCP 인증 아이스크림 업체와 미인증 업체의 미생물 관리 수준 비교)

  • Bae, Jin-Gyu;Jeon, Oh-Sook;Park, Hye-Won;Bok, Min-Soon;Yang, Ho-Yul;Bark, Jun-Jo;Ko, Tae-Oh
    • Korean Journal of Veterinary Service
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    • v.36 no.2
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    • pp.95-103
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    • 2013
  • The hazard analysis critical control point (HACCP) system was introduced to the Korean livestock slaughter and product processing business in 1997. Ice cream manufacturers focus on the sterilization process in order to reduce microorganism, so the HACCP-accredited ice cream manufacturers particularly set the sterilization process as critical control point (CCP). Therefore, the microbiological test results on the finished products can be considered as one of the indicators which show the level of microbiological control in the ice cream manufacturers. Accordingly, this study was carried out to examine if there is a meaningful difference in terms of the microbiological control level between the HACCP-accredited and non-accredited ice cream manufacturer groups by comparing aerobic plate count (APC) of ice creams. The mean of APC was 1.68 Log CFU/g in the HACCP-accredited ice cream manufacturer group and 2.59 Log CFU/g in the non-accredited ones respectively and there was a statistically significant difference (P<0.05). The test results suggest that the level of microbiological control in the HACCP-accredited ice cream manufactures is much higher than that of non-accredited ones.

MICROBIOLOGICAL QUALITY OF BRAZILIAN LIPSTICKS AFTER NORMAL USE BY CONSUMERS

  • D.M.M. Pedroso;G.R. Dias;Gesztesi, J-L
    • Proceedings of the SCSK Conference
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    • 2003.09b
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    • pp.524-526
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    • 2003
  • Lipsticks are cosmetics which do not contain water and are usually preserved with parabens. When submitted to the Challenge Test, theses products did not reach the CTFA criteria, which means that microbiological contamination could occur before the end of its shelf life. The aim of this study was to evaluate the contamination level of 130 lipsticks after its use. Microorganisms were isolated from 14,6 % of the samples. However, only in two samples (1,5%) the contamination level exceeded the 100 CFU/g level, which means that, although the preservative system was not efficient to eliminate bacteria, the lack of free water was enough to prevent the microbial development. Total bacteria and fungi were determined by conventional methodology, according to CTFA Microbiological Guidelines. The microbes were then isolated and characterized as normal skin flora microorganisms. This suggest that products were contaminated by the constant application of lipsticks by consumers. This could lead to cross contamination when the same product is shared by several people. Extra care should be taken into account when this type of products are available to be tested by several consumers in demonstration displays.

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Evaluation of Shelf-life of Bojungikgi-tang by Long-term Storage Test (장기보존시험에 따른 보중익기탕 전탕팩의 유통기한 평가)

  • Seo, Chang-Seob;Kim, Jung-Hoon;Kim, Seong-Sil;Lim, Soon-Hee;Shin, Hyeun-Kyoo
    • Korean Journal of Pharmacognosy
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    • v.44 no.2
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    • pp.200-208
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    • 2013
  • The aim of this study was to evaluate the shelf-life of Bojungikgi-tang (Buzhongyiqi-tang in Chinese) by long-term storage test. Experiments were performed to evaluate the stability such as the selected physicochemical, pH, identification, heavy metal, microbiological experiment, and amount of marker compounds under a long-term storage test of Bojungikgi-tang decoction. The significant change was not showed in pH, heavy metal, microbiological, and identification test based on long-term storage test. Furthermore, the HPLC analysis was performed for the determinations of liquiritin, glycyrrhizin, nodakenin, and hesperidin in Bojungikgi-tang by long-term storage test. We were calculated shelf-life of Bojungikgi-tang decoction based on the amount change of four constituents. Consequently, Shelf-life by four compounds at room temperature was predicted 23 month. The suggested shelf-life would be helpful on the storage and distribution of herbal medicine.

Establishment of Shelf-life of Ssanghwa-tang by Long-term Storage Test (장기보존시험에 따른 쌍화탕의 유통기한 설정)

  • Seo, Chang-Seob;Kim, Jung-Hoon;Lim, Soon-Hee;Shin, Hyeun-Kyoo
    • Korean Journal of Pharmacognosy
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    • v.43 no.3
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    • pp.257-264
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    • 2012
  • The purpose of this study was to estimate the shelf-life of Ssanghwa-tang by long-term storage test. Experiments were conducted to evaluate the stability such as the selected physicochemical, pH, identification, heavy metal, microbiological experiment under a long-term storage test of Ssanghwa-tang. The significant change was not showed in pH, heavy metal, microbiological, identification test and quantitative analysis based on long-term storage test. The contents of albiflorin, paeoniflorin, cinnamic acid, liquiritin, and glycyrrhizin in long-term storage test were 66.8-93.1 ${\mu}g/mL$, 429.0-495.0 ${\mu}g/mL$, 3.8-4.4 ${\mu}g/mL$, 32.0-38.1 ${\mu}g/mL$, and 66.8-71.7 ${\mu}g/mL$, respectively. Shelf-lifes by 5 compounds about 3 lots at room temperature were predicted 21-37, 14-21, and 16-72 months, respectively. The suggested shelf-life would be helpful on the storage and distribution of herbal medicine.

Experimental Study on the Irradiation and Surface Sterilization Effect of Ultra Violet Ray in Air Conditioning System (공조시스템에서 UV Ray의 조사 및 표면살균성능에 관한 실험적 연구)

  • 홍진관
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.16 no.3
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    • pp.250-257
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    • 2004
  • Recently, the use of UVC lamps inside building air-conditioning system has been increasing in both medical and nonmedical buildings for the control of environmental microorganisms. In the present study, irradiance performance test of UVC lamp was carried out and surface sterilization effect of UV ray was investigated by using UV ray irradiation experimental chamber and pilot system. Experimental results show that the effective irradiance of UVC lamp is strongly dependent on air velocity and temperature with exception of relative huminity in air-conditioning system. An individual microbiological kill effectiveness experiment also shows that the fractional kill of two microbiological samples such as E. Coli and Legionella is roughly the same as the estimated fractional kill in the case of chamber test and pilot system test.