• 한국염색가공학회지
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• 제11권2호
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• pp.19-26
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• 1999

Strains degrading and decolorizing acid dyes, Nylosan red E-BL 150%. were isolated from natural system, was named as ARK3. The optimal culture conditions of temperature and pH were $35^\circ{C}$, 7.0, respectively. Growth rate of cells in conditions of aerobic shaking more than standing culture conspicuously increased, and optical density of those to strain ARK3 were found as 1.38 and 0.25 after 42 hrs. Decolorization efficiency in batch culture which used as immobilization media to natural zeolite was 15% after 6 hrs, while suspension culture was 5%, also its of immobilization and suspension culture were 90% and 85% after 48 hrs, respectively. Decolorization efficiency of air-lift bioreactor was more than 90% to a dilution rate of $0.038hr^{-1}$, but that was decreased as 70%, when the dilution rate was $0.05hr^{-1}$. Even though at maximum dilution rate of this study, there was not appeared "wash out" phenomienon of biomass. Decolorization efficiency was 97.7% at a dilution rate of $0.025hr^{-1}$, when influent dye concentration was $100mg/\ell$. But if influent dye concentration increased as $150mg/\ell$, even though MLVSS increased, that of treatment water decreased as 93%. Also, when influent dye concentration increased as $200mg/\ell$ and $300mg/\ell$, decolorization efficiencies of treatment water abruptly decreased as 85% and 63%, respectively. Decolorization efficiency was more than 92% to the limit volumetric loading rate of $3.75mg/\ell\cdot{hr}$hr, without regard to variation of influent dye concentration or hydraulic retention time. if volumetric loading rate was more than $3.80mg/\ell\cdot{hr}$, at same condition, decolorization efficiency was lower decrease of retention time than increase of influent dye concentration.entration.

• 생명과학회지
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• 제14권4호
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• pp.577-581
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• 2004

Azo계 염색 폐수 처리에 활용되었던 활성 슬러지의 미생물 군집체로부터 diazo계 반응성 염료인 Reactive Black 5를 유일 탄소원으로 성장할 수 있는 Comamonas sp. AEBL-85를 분리ㆍ동정하고, Comamonas sp. AEBL-85를 이용한 Reactive Slacks에 대한 회분식 탈색 특성을 평가하였다. 염료탈색 반응 효율 향상을 위해 보조 탄소원 및 질소원 첨가하고, pH, 온도 등의 분해조건이 탈색율에 미치는 영향을 분석한 결과, 3% (w/v)의 포도당, 0.5% (w/v)의 yeast extract를 첨가한 MSM에서 pH 6.0, 온도 35$^{\circ}C$의 조건에서 탈색효율이 가장 높았다. 초기농도 50 mg/l의 Reactive Black 5에 대하여 40시간의 회분식 탈색반응을 통해 약 95% 이상의 탈색율을 얻을 수 있었다.

• 대한환경공학회지
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• 제28권7호
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• pp.738-745
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• 2006

본 연구에서는 염색폐수의 색도제거 기전을 미생물 floc에의 물리 화학적 흡착과 미생물의 대사에 의한 생물학적 제거의 두 가지로 분류하였다. 색도제거의 반응 조건, 즉 혐기/호기 조건, cosubstrate의 종류와 주입량 등을 회분식 실험에 의해 규명하고, 활성슬러지와 비활성슬러지의 biosorption 실험을 통하여 색도제거 기전을 확인하였다. 염색폐수의 색도는 호기조건과 혐기조건에서 각각 102 ${\Delta}$unit/g MLSS, 123 ${\Delta}$unit/g MLSS가 제거되어 혐기조건에서의 제거율이 높았으며, 유기물은 호기조건에서 82 $mg{\Delta}$COD/gMLSS, 혐기조건에서 75 $mg{\Delta}$COD/gMLSS 제거되어 호기조건에서 제거율이 더 높게 나타났다. Cosubstrate로서 실폐수인 가정하수와 acetate를 이용하여 주입량에 따른 염색폐수의 제거능을 분석한 결과, cosubsrate의 주입에 따라 색도 및 유기물 제거량이 증가함을 확인하였으며 가정하수보다는 acetate가 색도제거에 있어서 더 효율적인 cosubstrate임을 알 수 있었다. 활성슬러지와 멸균된 비활성슬러지를 이용한 색도제거 실험 결과. 비활성슬러지의 색도제거량은 가정하수의 주입에 따라 $20.3{\sim}37.3$ ${\Delta}$unit/g MLSS, 활성슬러지는 $102.0{\sim}159.0$ ${\Delta}$unit/g MLSS로 나타났다. 또한 반응 초기에는 물리 화학적 흡착이 우세하였으나 시간이 지나면서 생물대사 작용에 의한 제거가 증가하는 경향을 보였으며, cosubstrate의 주입에 따른 미생물의 대사에 의한 제거분율이 증가하는 것으로 분석되었고, 이는 호흡율 측정결과와도 그 경향이 일치하였다.

• Environmental Engineering Research
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• 제25권4호
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• pp.561-570
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• 2020

In the present study, dye decolorizing bacteria were isolated from water and soil samples, collected from textile industries in Jodhpur province, India. Two bacterial species namely, Bacillus pumilis and Paenibacillus thiaminolyticus were screened and identified based on biochemical characterization. The degradation efficiency of these two microorganisms was compared through optimization of pH, incubation time, initial dye concentration and inoculum size. B. pumilis and P. thiominolyticus were able to degrade 61% and 67% Red HE3B, 81% and 75% Orange F2R, 49.7% and 44.2% Yellow ME4GL and 61.6% and 59.5% Blue RC CT dyes of 800mg/l concentration respectively. The optimum pH and time were found to be 8 within 24 hours. The FT-IR analysis confirmed that microorganisms were able to degrade toxic azo dyes into a non-toxic product as proved through structural modifications to analyze chemical functions in materials by detecting the vibrations that characterize chemical bonds. It is based on the absorption of infrared radiation by the microbial product. Therefore, Bacillus pumilis and Paenibacillus thiaminolyticus are a promising tool for decolorization of dyes due to its potential to effectively decolorize higher azo dye concentrations (10-800 mg/L) and can be exploited for bioremediation.

• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.43-49
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• 2011

As a rare sugar alcohol, L-arabitol can be used in food and can prevent extra fat deposits in the intestinal tract. Commercially, L-arabitol is prepared from pure L-arabinose by hydrogenation, which needs a high temperature and high pressure, leading to a high production cost for Larabitol. Therefore, this study describes a novel L-arabitol production method based on biological purification from the xylitol mother liquor, a cheap and readily available raw material that contains a high concentration of Larabitol. First, a novel Bacillus megaterium strain was screened that can utilize xylitol, sorbitol, and mannitol, yet not L-arabitol. The isolated strain was inoculated into a medium containing the xylitol mother liquor under formulated culture conditions, where a high L-arabitol yield (95%) and high purity (80%) were obtained when the medium was supplemented with 50 g/l of xylitol mother liquor. Upon further purification of the fermentation broth by ion exchange and decolorization, L-arabitol was crystallized with a purity of 98.5%.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 하계학술대회 논문집 Vol.4 No.2
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• pp.818-821
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• 2003

Ozonic use of sterilization and heat treatment of raw material to extend the conservation period of food recently is increased by hard vegetable or microorganism sterilization purpose of fruit. If ozone can create as is easy comparatively because do air or oxygen by raw material and schedule period passes, there is advantage that do not leave the second contaminant because being gotten restored by oxygen. Also, because the effect is big to decolorization beside sterilization effect about microorganism, deodorization, disjointing of venomousness hazardous substance, food save, indoor air purge, good hand processing, hydrospace agricultural chemicals processing etc. the use extent is wide. This research ran parallel a sterilization experiment of E.coli colitis germs by ozone that manufacture ozonizer that use doped diamond maund electrode (BDD) by boron and searched special quality electrochemistry enemy of the ozonizer and is created. E.coli. After cultivate E.coli colitis germs during 37C 1 day is LB ship, after do ozonation, was sterilized more than 90% by ozone as result that examine sterilizing power by substance microscope and could confirm excellency of diamond electrode.

• 한국식품영양과학회지
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• 제35권1호
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• pp.15-20
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• 2006

백운3호, 대엽조생, 수원뽕, 청일뽕, 대축면, 강원3호, 희학, 카타네오, 국상20호 등 9가지 오디를 이용하여 항산화효과와 Helicobacter pylori에 대한 억제효과를 측정하였다. 페놀함량을 측정한 결과 물 추출물에서는 강원3호가 2.90 mg/g으로 다른 시료에 비하여 높게 나왔으며, $40\%$ ethanol 추출물에서도 강원3호가 3.02 mg/g으로 가장 높게 나타났다. 항산화효과 중 DPPH에 대한 전자공여능은 물 추출물에서 강원3호가 $99.54\%$로 가장 높게 나타났으며, $40\%$ ethanol 추출물에서는 대엽조생이 $89.61\%$로 나타났다. ABTS radical decolorlization을 측정한 결과 물 추출물에서는 청일뽕이 $96.14\%$로 가장 높게 나타났으며, $40\%$ ethanol추출물에서는 백운3호가 $99.9\%$로 나타났다. TBARS값은 control값이 $1.56\times10^{-2}{\mu}M$으로 나타났으며, 청일뽕이 물 추출물에서 가장 낮은 $0.86\times10^{-2}{\mu}M$값을, $40\%$ ethanol 추출물에서도 역시 청일뽕에서 $1.03\times10^{-2}{\mu}M$의 TBARS 값을 나타내어 산화촉진인자를 binding하는 능력이 $40\%$ ethanol 추출물보다 물 추출물이 좋은 것으로 나타났다. TBARS와 같이 지용성 물질의 항산화력을 나타내는 antioxidant protection factor에서는 청일뽕이 물 추출물에서 2.25로 나타났으며, $40\%$ ethanol 추출물에서는 대엽조생에서 1.49로 나타났다. 오디 추출물의 Helicobacter pylori에 대한 항균활성은 물 추출물에서 청일뽕과 국상20에서 강한 저해활성이 나타나 천연 항균제 및 생리활성 물질로의 이용가능성을 추측할 수 있었다.