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http://dx.doi.org/10.4491/eer.2019.224

Molecular identification of dye degrading bacterial isolates and FT-IR analysis of degraded products

Khan, Shellina (Department of Biosciences, Mody University of Science and Technology)
Joshi, Navneet (Department of Biosciences, Mody University of Science and Technology)

Publication Information

Environmental Engineering Research / v.25, no.4, 2020 , pp. 561-570 More about this Journal

Abstract

In the present study, dye decolorizing bacteria were isolated from water and soil samples, collected from textile industries in Jodhpur province, India. Two bacterial species namely, Bacillus pumilis and Paenibacillus thiaminolyticus were screened and identified based on biochemical characterization. The degradation efficiency of these two microorganisms was compared through optimization of pH, incubation time, initial dye concentration and inoculum size. B. pumilis and P. thiominolyticus were able to degrade 61% and 67% Red HE3B, 81% and 75% Orange F2R, 49.7% and 44.2% Yellow ME4GL and 61.6% and 59.5% Blue RC CT dyes of 800mg/l concentration respectively. The optimum pH and time were found to be 8 within 24 hours. The FT-IR analysis confirmed that microorganisms were able to degrade toxic azo dyes into a non-toxic product as proved through structural modifications to analyze chemical functions in materials by detecting the vibrations that characterize chemical bonds. It is based on the absorption of infrared radiation by the microbial product. Therefore, Bacillus pumilis and Paenibacillus thiaminolyticus are a promising tool for decolorization of dyes due to its potential to effectively decolorize higher azo dye concentrations (10-800 mg/L) and can be exploited for bioremediation.

Keywords

Bioremediation; Characterization; Decolorization; Dyes; FT-IR;

Citations & Related Records

Reference

1	Sarkar S, Banerjee A, Halder U, Biswas R, Bandopadhyay R. Degradation of synthetic Azo Dyes of textile industry: A Sustainable Approach Using Microbial Enzymes. Water Conserv. Sci. Eng. 2017;2:121-131. DOI
2	Asad S, Amoozegar MA, Pourbabaee AA, Sarbolouki MN, Dastghei SMM. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresour. Technol. 2007;98:2082-2088. DOI
3	Guadie A, Tizazu S, Melese M, Guo W, Ngo HH, Xia S. Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake. Biotechnol. Rep. 2017;15:92-100. DOI
4	Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: A review. J. Taiwan Inst. Chem. Eng. 2011;42:138-157. DOI
5	Ramalho PA, Cardoso MH, Cavaco-Paulo A, Ramalho MT. Characterization of azo reduction activity in a novel ascomycete yeast strain. Appl. Environ. Microbiol. 2004;70:2279-2288. DOI
6	Wang H, Su JQ, Zheng XW, Tian Y, Xiong XJ, Zheng TL. Bacterial decolorization and degradation of the reactive dye Reactive Red 180 by Citrobacter sp. CK3. Int. Biodeterior. Biodegrad. 2009;63:395-399. DOI
7	Banat IM, Nigam P, Singh D, Marchant R. Microbial decolorization of textile dye containing effluents: A review. Bioresour. Technol. 1996;58:217-227. DOI
8	Singh L, Singh VP. Textile dyes degradation: A microbial approach for biodegradation of pollutants. Microbial degradation of synthetic dyes in wastewaters. Environ. Sci. Eng. 2015;187-204.
9	Arora S, Sain HS, Singh K. Decolorization optimization of a mono azo disperse dye with Bacillus firmus: Identification of a degradation product. Color. Technol. 2007;123:184-190. DOI
10	Chen K, Wu J, Liou D, Hwang S. Decolorization of the textile dyes by newly isolated bacterial strains. J. Biotechnol. 2003;101:57-68. DOI
11	Munesh K, Maulin PS, Swaranjit SC. Bioremediation of Remazol Black B by newly isolated Bacillus endophyticus LWIS strain. Adv. Biotech. Micro. 2016;1:001-007.
12	Forss J, Lindh MV, Pinhassi J, Welander U. Microbial biotreatment of actual textile wastewater in continuous sequential Rice Husk Biofilter and Microbial Community Involved. PLoS One 2017;12:1-16.
13	Cervantes F, Dos Santa A. Reduction of azo dyes by anaerobic bacteria: Morphological and biochemical aspects. Rev. Environ. Sci. Biotechnol. 2011;10:125-137. DOI
14	Khaled A, Nagui S, Abdel- Khalek A, El- Sayed SM, Abdullah SS. Bioremoval of crystal violet dye from Egyptian textile dye effluent. Int. Res. J. Eng. Technol. 2015;2:1038-1043.
15	Joshi PA, Jaybhaye S, Matre K. Biodegradation of dyes using consortium of bacterial strains isolated from textile effluent. Euro. J. Exp. Biol. 2015;5:36-40.
16	Geetha N, Illakkiam D, Subha D, Ahila V. Decolorization of Alizarin Red dye by bacterial strains isolated from Industrial effluents. Int. J. Plant Animal Environ. Sci. 2016;6:268-275.
17	Khaled A, Arshad J, Mahmood S, Aziz I, Arshad M. Effect of chromium forms on the biodegradation of reactive black-5 azo dye by Psychrobacter and Klebsiella species. Int. J. Agric. Biol. 2015;17:1260-1264. DOI
18	Chand N, Sajedi RH, Nateri AS, Khajeh K, MRassa. Fermentative desizing of cotton fabric using alpha-amylase-producing Bacillus strain: Optimization of simultaneous enzyme production and desizing. Process Biochem. 2014;49:1884-1888. DOI
19	Rajeswari K, Subashkumar R, Vijayaraman K. Biodegradation of mixed textile dyes by Bacterial strains isolated from dyewaste effluent. Res. J. Environ. Toxicol. 2011;5:97-107. DOI
20	Olukanni OD, Osuntoki AA, Awotula AO, Kalyani DC, Gbenle GO, Govindwar sp. Decolorization of dye house effluent and biodegradation of Congo red by bacillus thuringiensis RUN1. J. Microbiol. Biotechnol. 2013;23:843-849. DOI
21	Cho YG, Rhee SK, Lee ST. Influence of environment parameters on bioremediation of chlorophenol-contaminated soil by indigenous microorganisms. Environ. Eng. Res. 2000;5:165-173
22	Dey U, Chetterjee S, Mondal NM. Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation. Biotechnol. Reports 2016;10:1-7. DOI
23	Khadijah O, Lee KK, Mohd FF Abdullah. Isolation, screening and development of local bacterial consortia with azo dyes decolourising capability. Malaysian. J. Micro. 2009;5:25-32.
24	Pelczar MJ, Bard RC, Burnett GW, et al. Manual of microbiological methods. Society of American Bacteriology. New York: McGraw-Hill; 1957; p. 315.
25	Aoshima I, Tozawa Y, Ohmomo S, Ueda K. Production of decolorizing activity for molasses pigment by Coriolus versicolor Ps4a. Agric. Biol. Chem. 1985;49:2041-2045. DOI
26	Talavera G, Castresana J. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst. Biol. 2007;56:564-577. DOI
27	Telke AA, Joshi SM, Jadhav SU, Tamboli DP, Govindwar SP. Decolorization and detoxification of Congo Red and textile industry effluent by an isolated bacterium Pseudomonas sp. SU-EBT. Biodegradation 2010;21:283-296. DOI
28	Shah MP, Patel KA, Nair SS, Darji AM, Maharaul SJ. Microbial decolorization and degradation of Orange 16 dye by a newly isolated Aeromonas spp. Etl-1949. J. Bioremed. Biodeg. 2013;4:194.
29	Lone TA, Revathi C, Lone RA. Isolation of dye degrading Bacillus species from the soil near dyeing industry and its potential application in dye effluent treatment. Am-Euras. J. Toxicol. Sci. 2015;7:129-135.
30	Feng J, Cerniglia CE, Chen H. Toxicological significance of azo dye metabolism by human intestinal micro biota. Front Biosci. 2012;1:568-586. DOI
31	Govindasamy V, Senthilkumar M, Magheshwaran V, Upendra K, Bose P, Sharma V. Bacillus and Paenibacillus spp.: Potential PGPR for sustainable agriculture. In: Maheshwari DK, eds. Plant Growth and Health Promoting Bacteria, Microbiology Monographs 18. Berlin: Springer-Verlag; 2010. p. 334-364.
32	Edgar RC. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004,32:1792-1797. DOI
33	Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H. The redox- sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis. Proteomics 2010;10:3155-3164. DOI
34	Leena R, Raj SD. Bio-decolourization of textile effluent containing reactive Black-B by effluent-adapted and non-adapted bacteria. Afr. J. Biotechnol. 2008;7:3309-3313.
35	Olukanni OD, Osuntoki AA, Gbenle GO. Textile effluent biodegradation potentials of textile effluent-adapted and non-adapted bacteria. Afr. J. Biotechnol. 2006;5:1980-1984.
36	Mohan V, Madhumitha M, Menon S, Saranya DK. Isolation and Screening of potential Dye decolorizing bacteria from Textile dye effluents in Tamil Nadu. Indian J. Acad. Ind. Res. 2013;2:3678-3783.
37	Prasad ASA, Rao KVB. Aerobic biodegradation of Azo dye acid black-24 by Bacillus halodurans. J. Environ. Biol. 2014;35:549-554.
38	Anjaneya O, Souche SY, Santoshkumar M, Karegoudar TB. Decolorization of sulfonated azo dye Metanil Yellow by newly isolated bacterial strains: Bacillus sp. strain AK1 and Lysinibacillus sp. strain AK2. J. Hazard. Mater. 2011;190:351-358. DOI
39	Ramya M, Anusha B, Kalavathy S. Decolorization and biodegradation of indigo carmine by a textile soil isolate Paenibacillus larvae. Biodegradation 2008;19:283-291. DOI
40	Ahmed F, Dewani R, Pervez MK, Mahboob SJ, Soomro SA. Non-destructive FT-IR analysis of mono azo dyes. Bulgarian Chem. Comm. 2016;48:71-77.
41	Sarayu K, Sandhya S. Aerobic biodegradation pathway for Remazol Orange by Pseudomonas aeruginosa. Appl. Biochem. Biotechnol. 2010;160:1241-1253. DOI
42	Ayaz N, Thankappan R, Srinivasan SV, TamilSelvi A. Studies on decolorisation of Acid Blue 113 using Staphylococcus Aureus and Escherichia Coli isolated from tannery wastewater. 2015;4:938-948. DOI