• Title/Summary/Keyword: microbial content

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A study for the Quality Depending on Sanitization and Storage Method of Raw Vegetables in Foodservice Operation (단체급식소에서 이용되는 일부 생채소의 소독방법 및 저장에 따른 품질연구)

  • Kim Heh-Young
    • Korean journal of food and cookery science
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    • v.20 no.6 s.84
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    • pp.684-694
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    • 2004
  • The purpose of this study was to estimate the microbial and physicochemical quality of some raw vegetables and suggest safer methods of sanitization and storage for foodservice operations. Three sanitization methods were utilized during pre-preparation (tap water, chlorine water and electrolyzed water). Leek and Chicory were monitored as ingredient, with different storage temperatures $(3,\;10^{\circ}C)$ and periods (1, 2, 3, 4 and 7 days). The largest reduction in the microbial counts was shown with the electrolyzed water and for the case before immersion in chlorine water, performing a first washing was more effective in reducing the microbial counts than with no washing. The results showed that the storage temperature, pH, moisture content and microbial loads were important factors affecting the quality of vegetables.

Environmental Change and Its Enhancement of a Bay Sediment by Using Useful Microbial and Chemical Treatments (연안저질 환경 개선을 위한 유용 미생물제제 및 산화제의 사용에 따른 환경변화 및 효율성 관찰)

  • Cho, Dae-Chul;Bae, Hwan-Jin;Lee, Jung-Yeol;Kwon, Sung-Hyun
    • Journal of Environmental Science International
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    • v.19 no.11
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    • pp.1355-1362
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    • 2010
  • This study was carried out in order to observe how the bay sediment would be changed with microbial treatments and a chemical oxidant like $CaO_2$. The sediment during the treatments was analyzed in terms of pH, ORP, volatile organics content, COD, AVS, T-N, and T-P. With $CaO_2$ treatment, pH was kept over 9.66 and ORP ranged from +4.70~+46.0, which meant an aerobic state meanwhile with the microbial treatment those were worse. In addition the chemical treatment showed better environmental index values than the microbial one: volatile organics content and COD values in the former were 12.9% and 37.9% while those in the latter were 4.5% and 18.7%, respectively. AVS and T-P were 71.1% and 100% versus 56.5% and 85.8%, respectively. However, the microbial treatment was better for T-N(66% higher). On the other hand, both treatment at a time enhanced all the environmental indices but COD meantime pH and ORP values were lower than with the chemical treatment only. Thus additional input of an oxygen generator like $CaO_2$ could improve the environmental state of a bay sediment where the biological treatment is going on.

Effects of TLB Microbial fertilizer application on Soil Chemical Properties, Microbial Flora and Growth of Chniese Cabbage (Brassica Compestris subsp. napus var. pekinensis MAKINO) (미생물제 비료시용이 배추의 생육과 토양 화학성 및 미생물상에 미치는 영향)

  • Yun, Sei-Young;Shin, Joung-Du
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.1
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    • pp.8-16
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    • 2001
  • This experiment was conducted to investigate the effects on plant growth, soil chemical properties and microbial flora with microbial fertilizer to chinese cabbage cultivation. The plant growth was promoted and the yield increased by application of Tian Li Bao(TLB) microbial fertilizer as compared with the control. However, yield a littler decreased in case of the reduced amount of urea application as a top dressing and half of compost chicken manure as a basial fertilizer even if treated with TLB microbial fertilizer. Organic matter and total nitrogen contents decreased as compared to those in the control, and total nitrogen ranged in 0.76~1.44% in the treatments at harvesting time, and decreased with application of TLB microbial fertilizer compared to that of the control. The available phosphorus content in the field before experiment was 559ppm, but it was 755ppm and 653 in the control and treatments at harvesting time, respectively. Therefore, it was shown that phosphorus content in the treatment was lower than that of the control. On the other hand, total nitrogen, phosphorous and K ranged from 2.62 to 2.94%, from 1.48 to 1.55% and from 3.60 to 4.38% in plants after harvest, respectively. There were no significant differences among the treatments. For the soil microbial flora, the population of bacteria in the treatments decreased with application of microbial fertilizer as compared with the control over all cultivation periods. It was shown that the population of pseudomonas spp. was over 3 times higher than that of the control after harvesting. The population of actinomycetes didn't show difference among the treatments, but high density of fungi after harvesting were observed in the treatments.

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Evaluation of Fluoride Removal Effect with Growth of Attached Microbial Community in Middle and Small Stream (중·소하천에서 부착미생물군집의 성장에 따른 불소 제거 효과 평가)

  • Kim, Tae-Kyung;Ryu, Seo-Young;Park, Yoon-A;Lee, Jong-Jun;Joo, Kwang-Jin;Chang, Kwang-Hyeon;Oh, Jong-Min
    • Journal of Environmental Impact Assessment
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    • v.27 no.6
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    • pp.595-603
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    • 2018
  • This study was conducted to understand the growth of attached microbial community in small and medium streams of Gyeonggi-do and the removal efficiency of fluoride with changes in growth. The sites monitoring were carried out for 12 weeks at the P1 and P2(P1 is the point where the discharge water flows. P2 is the downstream point of about 2 km flow), and investigated the water quality and the biomass analysis of the attached microbial community. The growth of the attached microbial community increased up to the 7th investigation, and it was observed that detachment phenomenon occurred. Influencing factors about growth of the attached microbial community were flow rate, velocity, and organic material(T-N & T-P). Meanwhile, fluoride content of attached microbial community also tended to increase until the 7th investigation, and decrease from the 8th. It is assumed that fluoride content has also be reduced with the detachment phenomenon of the attached microbial community. It is expected that this will contribute to the evaluation and management of the use of attached microbial community as a means of stream management. The application of techniques using the attached microbial community should include basic investigation of factors that may affect the growth of the attached microbial community and replacement of the attachment plate according to the time of removal.

Development of Models for the Prediction of Domestic Red Pepper (Capsicum annuum L.) Powder Capsaicinoid Content using Visible and Near-infrared Spectroscopy

  • Lim, Jongguk;Mo, Changyeun;Kim, Giyoung;Kim, Moon S.;Lee, Hoyoung
    • Journal of Biosystems Engineering
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    • v.40 no.1
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    • pp.47-60
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    • 2015
  • Purpose: The purpose of this study was to non-destructively and quickly predict the capsaicinoid content of domestic red pepper powders from various areas of Korea using a pungency measurement system in combination with visible and near-infrared (VNIR) spectroscopic techniques. Methods: The reflectance spectra of 149 red pepper powder samples from 14 areas of Korea were obtained in the wavelength range of 450-950 nm and partial least squares regression (PLSR) models for the prediction of capsaicinoid content were developed using area models. Results: The determination coefficient of validation (RV2), standard error of prediction (SEP), and residual prediction deviation (RPD) for the capsaicinoid content prediction model for the Namyoungyang area were 0.985, ${\pm}2.17mg/100g$, and 7.94, respectively. Conclusions: These results show the possibility of VNIR spectroscopy combined with PLSR models in the non-destructive and facile prediction of capsaicinoid content of red pepper powders from Korea.

Ferulic Acid Content of Barley and Wheat Grains and Head Blight Resistance (맥류 종자의 페룰산(ferulic acid) 함량과 붉은곰팡이 저항성)

  • Baek, Seul Gi;Kim, Sosoo;Jang, Ja Yeong;Kim, Jeomsoon;Lee, Theresa
    • Research in Plant Disease
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    • v.26 no.4
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    • pp.250-255
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    • 2020
  • In order to find if a ferulic acid (FA) can be used as a selection index in cereal breeding for resistance to head blight and mycotoxin production, we analyzed FA in the grains of 80 cultivars of barley, rice, and wheat. FA content ranged 1.66-2.77 mg/g in barley (n=20), 0.56-1.53 mg/g in wheat (n=40), and 0.91-2.13 mg/g in rice (n=20). Among these, 7 cultivars each of barley and wheat with different FA content were tested for head blight and mycotoxin production by 2 Fusarium graminearum and 2 F. asiaticum strains. Mean pathogenicity of the wheat cultivars was significantly less than that of barley with higher FA and among wheat cultivars, there was no correlation between FA content and pathogenicity. Mycotoxin production was also lower in the wheat than in the barley as pathogenicity. However, pathogenicity and toxins produced by F. asiaticum were negatively correlated with FA content in barley. These results indicate that FA is not a resistance factor to head blight by F. asiaticum and F. graminearum or its mycotoxin production in barley and wheat.

Studies on the Microbial Population and the Amylase Activity of the Forest Soil (삼림토양의 미생물군집과 아밀라아제 활성에 관한 연구)

  • Lee, Hee-Sun;Shim, Jae-Kuk
    • The Korean Journal of Ecology
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    • v.17 no.2
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    • pp.171-183
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    • 1994
  • Soil condition, total number of bacteria, soil amylase activity and microbial biomass $(CO_2-C)$ were measured at soil of different forest types. And the difference of the allelopathic effect was determined between fresh leaf extract of Quercus acutissima and Pinus rigida to the bacteria isolated from soil of different forest types. 1. Total number of bacteria in Carpinus laxiflora forest soil was 4~7 times larger than that in pinus desiflora forest soil. 2. Soil amylase activity was positively correlated with total number of soil bacteria and soil organic matter content. The amylase activity at F layer was 4~5 times larger than that at H layer, and that at H layer was 2~4 times larger than that at A layer. 3. Seasonal changes of microbial biomass showed a peak in summer, and vertical distribution of microbial biomass decreased with increasing soil depth. The microbial biomass in Pinus densiflora forest soil was larger than that in Quercus serrata forest soil. 4. Fresh leaf extract of Pinus rigida and Quercus acutissima showed an acceleration or inhibition effect on the growth of soil bacteria, and that of !. acutissima inhibited larger number of soil bacterial strains than that of P. rigida. 4.2% and 25% of soil bacterial strains isolated from soil of P. rigida and Q. acutissima forests were inhibited by fresh leaf extract of P. rigida and Q. acutissima, respectively.

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Enhancement of Microbial Immobilization on the Surface of a Reticulated PU-g-PAAc Foam prepared through Graft Copolymerization induced by Atmosoheric Pressure Plasma Treatment (대기압 플라즈마 유도 그라프트 공중합으로 합성된 망상형 PU-g-PAAc 폼의 미생물 고정화능 향상)

  • Myung Sung Woon;Jang Yung Mi;Nam Ki Chun;Choi Ho Suk;Cho Dae Chul
    • KSBB Journal
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    • v.19 no.5
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    • pp.399-405
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    • 2004
  • A reticulated PU-g-PAAc foam was modified through the surface treatment of PU foam by one atmospheric pressure plasma. The synthesized PU-g-PAAc foam was prepared for the purpose of immobilizing microbial organisms. We also attempted different plasma treatment methods including simple plasma treatment, plasma induced grafting and plasma induced grafting followed by plasma re-treatment. The effect of grafting on equilibrium water content (EWC) of PU forms was examined by swelling measurements. Adhesion test was performed to investigate the effect of different plasma treatment methods on the improvement of microbial immobilization. Two foams modified by plasma induced grafting and plasma re-treatment after grafting showed 2.7 and 3.0 fold higher microbial immobilization than unmodified one, respectively. Meanwhile, simple plasma treatment showed a little enhancement. FT-IR analysis of each sample verified the contribution of surface functional groups on the enhancement of microbial immobilization. SEM observation confirmed microbial adherence.

Potential Application of the Recombinant Escherichia coli-Synthesized Heme as a Bioavailable Iron Source

  • Kwon, Oh-Hee;Kim, Su-Sie;Hahm, Dae-Hyun;Lee, Sang-Yup;Kim, Pil
    • Journal of Microbiology and Biotechnology
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    • v.19 no.6
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    • pp.604-609
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    • 2009
  • To investigate the potential use of microbial heme as an iron source, recombinant Escherichia coli coexpressing ALA synthase (HemA) as well as the NADP-dependent malic enzyme (MaeB) and dicarboxylic acid transporter (DctA) were cultured. The typical red pigment extracted from the recombinant E. coli after 38 h showed highest absorbance at 407 nm, and the amount of iron in 38.4 mg of microbial heme extract derived from 6-1 fermentation broth was 4.1 mg. To determine the commercial potential of the recombinant E.coli-synthesized iron-associated heme as an iron source, mice were fed the iron-free provender with the microbial heme extract. The average body weight reduction of mice fed non-iron provender was 2.3%, whereas no detectable weight loss was evident in mice fed microbial heme addition after 15 days. The heme content of the blood from microbial heme fed mice was 4.2 mg/ml whereas that of controls was 2.4 mg/ml, which implies that the microbial heme could be available for use as an animal iron source.

Enhancement of Polyphenol Content and Antioxidant Activity of Brown Alga Eisenia bicyclis Extract by Microbial Fermentation

  • Eom, Sung-Hwan;Kang, Young-Mi;Park, Jae-Hong;Yu, Dae-Ung;Jeong, Eun-Tak;Lee, Myung-Suk;Kim, Young-Mog
    • Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.192-197
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    • 2011
  • The objective of this study was to select an effective microbial strain to improve the functional qualities of Eisenia bicyclis water extract by fermentation. For this purpose, several microorganisms isolated from traditional Korean fermented foods were inoculated and cultivated in E. bicyclis water extract. Ultimately, yeast strain YM-1 was selected for further study based on its total phenolic compound (TP) content and antioxidant activity, which were enhanced by microbial fermentation. The extract fermented by YM-1 exhibited a superior TP content and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to extracts fermented by other microbes. The highest TP content and DPPH radical scavenging activity were observed after one day of YM-1 fermentation. Yeast strain YM-1 was identified as Candida utilis based on an analysis of its physiological characteristics. During fermentation of the extract by C. utilis YM-1, no significant difference was observed in the proximate composition, including moisture, crude lipid, crude protein, and crude ash. Fermentation by C. utilis YM-1 resulted in enhanced biological activity, including increases in the TP content and antioxidant activity. Thus, fermentation by C. utilis YM-1 is an attractive strategy for developing value-added food ingredients.