• Title/Summary/Keyword: microCT

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A Study on the Usefulness of 3D Imaging in Micro-CT for Observing the Microstructure of Mice (흰쥐 미세구조 관찰을 위한 Micro-CT 3D 영상의 유용성에 관한 연구)

  • Lee, Sang-Ho;Lee, Jong-Seok;Lim, Cheong-Hwan;Jung, Hong-Ryang;Chai, Kyu-Yun;Han, Beom-Hee;Han, Sang-Hyun;Hong, Dong-Hee;Han, Tae-Jong;Park, Sung-Hoon;Mo, Eun-Hee;Jun, Hong-Young
    • Journal of Digital Convergence
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    • v.12 no.3
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    • pp.367-375
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    • 2014
  • In this thesis we observe microvascular structure in mice by using micro-computed tomography (CT), which is high-resolution X-ray imaging equipment that can acquire Real-time dynamic image, and it aims to investigate the usefulness of micro-CT developed by Institute for Radiological Imaging Science Wonkwang University School of Medicine. After acquiring the systemic images of rats, contrast-enhanced 3D images of vascular structures could be acquired by using Maximum Intensity Projection (MIP) and Volume Rending Technique (VRT), This was divided into each vascular system of head, abdomen and heart and systemic vascular system.

A study on the micro-structural and biomechanical properties of trabecular bone in intertrochanteric region (대퇴골 전자간부 해면골의 미세구조적 특성과 생역학적 특성에 관한 연구)

  • Baek M.H.;Won Y.Y;Cui W.Q.;Kim K.K;Kim H.S.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.06a
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    • pp.923-926
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    • 2005
  • To investigate the relationship between BMD, micro-structural and mechanical properties in intertrochanteric trabecular bone, the PIXI-mus2 system, micro-CT and FE model were used. The purpose of this study were (1) to apply high-resolution imaging techniques (micro-CT imaging) in combination with new computer modeling techniques (FEA) to quantify 3D microstructural and biomechanical properties of trabecular bone in the intertrochanteric region, and (2) determine if the prediction of bone elastic constant can be improved with structural index.

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3D micro-CT analysis of void formations and push-out bonding strength of resin cements used for fiber post cementation

  • Uzun, Ismail Hakki;Malkoc, Meral Arslan;Keles, Ali;Ogreten, Ayse Tuba
    • The Journal of Advanced Prosthodontics
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    • v.8 no.2
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    • pp.101-109
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    • 2016
  • PURPOSE. To investigate the void parameters within the resin cements used for fiber post cementation by micro-CT (${\mu}CT$) and regional push-out bonding strength. MATERIALS AND METHODS. Twenty-one, single and round shaped roots were enlarged with a low-speed drill following by endodontic treatment. The roots were divided into three groups (n=7) and fiber posts were cemented with Maxcem Elite, Multilink N and Superbond C&B resin cements. Specimens were scanned using ${\mu}CT$ scanner at resolution of $13.7{\mu}m$. The number, area, and volume of voids between dentin and post were evaluated. A method of analysis based on the post segmentation was used, and coronal, middle and apical thirds considered separately. After the ${\mu}CT$ analysis, roots were embedded in epoxy resin and sectioned into 2 mm thick slices (63 sections in total). Push-out testing was performed with universal testing device at 0.5 mm/min cross-head speed. Data were analyzed with Kruskal-Wallis and Mann-Whitney U tests (${\alpha}=.05$). RESULTS. Overall, significant differences between the resin cements and the post level were observed in the void number, area, and volume (P<.05). Super-Bond C&B showed the most void formation ($44.86{\pm}22.71$). Multilink N showed the least void surface ($3.51{\pm}2.24mm^2$) and volume ($0.01{\pm}0.01mm^3$). Regional push-out bond strength of the cements was not different (P>.05). CONCLUSION. ${\mu}CT$ proved to be a powerful non-destructive 3D analysis tool for visualizing the void parameters. Multilink N had the lowest void parameters. When efficiency of all cements was evaluated, direct relationship between the post region and push-out bonding strength was not observed.

Spiral scanning imaging and quantitative calculation of the 3-dimensional screw-shaped bone-implant interface on micro-computed tomography

  • Choi, Jung-Yoo Chesaria;Choi, Cham Albert;Yeo, In-Sung Luke
    • Journal of Periodontal and Implant Science
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    • v.48 no.4
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    • pp.202-212
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    • 2018
  • Purpose: Bone-to-implant contact (BIC) is difficult to measure on micro-computed tomography (CT) because of artifacts that hinder accurate differentiation of the bone and implant. This study presents an advanced algorithm for measuring BIC in micro-CT acquisitions using a spiral scanning technique, with improved differentiation of bone and implant materials. Methods: Five sandblasted, large-grit, acid-etched implants were used. Three implants were subjected to surface analysis, and 2 were inserted into a New Zealand white rabbit, with each tibia receiving 1 implant. The rabbit was sacrificed after 28 days. The en bloc specimens were subjected to spiral (SkyScan 1275, Bruker) and round (SkyScan 1172, SkyScan 1275) micro-CT scanning to evaluate differences in the images resulting from the different scanning techniques. The partial volume effect (PVE) was optimized as much as possible. BIC was measured with both round and spiral scanning on the SkyScan 1275, and the results were compared. Results: Compared with the round micro-CT scanning, the spiral scanning showed much clearer images. In addition, the PVE was optimized, which allowed accurate BIC measurements to be made. Round scanning on the SkyScan 1275 resulted in higher BIC measurements than spiral scanning on the same machine; however, the higher measurements on round scanning were confirmed to be false, and were found to be the result of artifacts in the void, rather than bone. Conclusions: The results of this study indicate that spiral scanning can reduce metal artifacts, thereby allowing clear differentiation of bone and implant. Moreover, the PVE, which is a factor that inevitably hinders accurate BIC measurements, was optimized through an advanced algorithm.

Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry

  • Kim, Jia;Song, Young-Sang;Min, Kyung-San;Kim, Sun-Hun;Koh, Jeong-Tae;Lee, Bin-Na;Chang, Hoon-Sang;Hwang, In-Nam;Oh, Won-Mann;Hwang, Yun-Chan
    • Restorative Dentistry and Endodontics
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    • v.41 no.1
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    • pp.29-36
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    • 2016
  • Objectives: The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.

Comparison of micro-computerized tomography and cone-beam computerized tomography in the detection of accessory canals in primary molars

  • Acar, Buket;Kamburoglu, Kivanc;Tatar, Ilkan;Arikan, Volkan;Celik, Hakan Hamdi;Yuksel, Selcen;Ozen, Tuncer
    • Imaging Science in Dentistry
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    • v.45 no.4
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    • pp.205-211
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    • 2015
  • Purpose: This study was performed to compare the accuracy of micro-computed tomography (CT) and cone-beam computed tomography (CBCT) in detecting accessory canals in primary molars. Materials and Methods: Forty-one extracted human primary first and second molars were embedded in wax blocks and scanned using micro-CT and CBCT. After the images were taken, the samples were processed using a clearing technique and examined under a stereomicroscope in order to establish the gold standard for this study. The specimens were classified into three groups: maxillary molars, mandibular molars with three canals, and mandibular molars with four canals. Differences between the gold standard and the observations made using the imaging methods were calculated using Spearman's rho correlation coefficient test. Results: The presence of accessory canals in micro-CT images of maxillary and mandibular root canals showed a statistically significant correlation with the stereomicroscopic images used as a gold standard. No statistically significant correlation was found between the CBCT findings and the stereomicroscopic images. Conclusion: Although micro-CT is not suitable for clinical use, it provides more detailed information about minor anatomical structures. However, CBCT is convenient for clinical use but may not be capable of adequately analyzing the internal anatomy of primary teeth.

Micromorphological Analysis of Primary Second Molar with Molar-incisor Malformation : A Case Report (Molar-incisor malformation에 이환된 제2유구치의 미세 형태학적 분석 : 증례 보고)

  • Kim, Myunghwan;Park, Howon;Lee, Juhyun;Seo, Hyunwoo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.48 no.3
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    • pp.352-358
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    • 2021
  • Molar-incisor malformation (MIM) is characterized by malformation in the root with a normal crown. While MIM mostly occurs in the permanent first molar, it has also been reported in the maxillary central incisor and the primary second molar (PSM), but anatomical analysis of the primary teeth with MIM has not been studied to date. In this case report, a patient with MIM was reported, and an extracted PSM with MIM was analyzed with micro computed tomography (CT). A cervical constriction morphology of the cementoenamel junction (CEJ) can be observed in extracted PSM. In micro CT analysis, characteristics such a mineralized plate (cervical mineralized diaphragm) in the CEJ area, complex root canal morphologies, a calcified mass inside the pulp chamber, and constricted pulp chamber of crown portion were observed.

Investigation of Microstructural Characteristics of Foamed Concrete with Different Densities (미세구조 분석방법을 활용한 서로 다른 밀도를 가진 기포콘크리트 특성 분석)

  • Chung, Sang-Yeop;Jo, Su-Sung;Oh, Seo-Eun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.43 no.1
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    • pp.1-7
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    • 2023
  • A set of foamed concrete specimens with different densities were prepared, and several microscopic techniques, such as scanning electron microscope (SEM) and X-ray micro-computed tomography (micro-CT) were used to characterize the foamed specimens. The pore and solid characteristics of the specimens at different ages were examined to investigate the effect of aging on the materials. The compressive strength and the thermal conductivity of the foamed specimens were also evaluated, and the relationship between the material characteristics and properties was integrated to identify the effect of density and aging on the material properties.

Microvascular Contrast Image in Portal Veins of Rat using Micro-CT (마이크로 CT를 이용한 BALB/C(흰쥐) 간문맥의 미세혈관 조영 영상)

  • Lee, Sang-Ho;Lim, Cheong-Hwan;Jung, Hong-Rayng;Han, Beom-Hee;Mo, Eun-Hee;Chai, Kyu-Yun
    • The Journal of the Korea Contents Association
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    • v.10 no.9
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    • pp.259-266
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    • 2010
  • The study focuses on the value of Micro CT, a high resolution X-ray imaging device, by using it on rats to observe the overall portal vein image of the liver and the microvasculature of each lobes, visualize the 4 segmental lobes and acquire 3D image of the microvasculature through the reconstruction of sectional image data. Less of the damage to liver of the 5 mice, the device was able to separate the liver into 4 segmental lobes and displayed the 4 portal vein microvasculature in 2D. By using the 3D MIP technique, observation of the whole portal vein system microvasculature in 3D image was made possible along with each of the portal vein segment's branches until the 6th branch. Measured the size of 6branch, the average was measured at 1branch : $0.51mm{\pm}0.08$, 2 branch : $0.32mm{\pm}0.12$, 3 branch : $0.23mm{\pm}0.11$, 4 branch : $0.19mm{\pm}0.08$, 5 branch : $0.13mm{\pm}0.06$, 6 branch : $70.5{\mu}m{\pm}14.1$. The 3D image and the images of the microvasculatures in the result of study proved that the Micro-CT can be considered many useful device in obtaining high resolution images.

Wahsing Effect of Micor-Bubbles and Changes in Quality of Lettuce (Lacutuca sativa L.) during Storage (마이크로버블에 의한 상추의 세척효과 및 저장 중 품질변화)

  • Lee, Seon-Ah;Youn, Aye-Ree;Kwon, Ki-Hyun;Kim, Byeong-Sam;Kim, Sang-Hui;Cha, Hwan-Soo
    • Food Science and Preservation
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    • v.16 no.3
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    • pp.321-326
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    • 2009
  • We assessed quality changes in and washing effects (time and method) on lettuce (Lactuca sativa L.) treated with micro-bubbles. Samples were treated with micro-bubbling for 1, 3, or 5 min, and the 5-min treatment yielded the best results in terms of reduced total microorganism counts, sensory aspects, and degree of washing. Total microorganism counts were 4.30 log colony-forming units (CFU)/g in unwashed lettuce(CT), 4.10 log CFU/g in hand-washed lettuce (HW), 3.98 log CFU/g in conventional, bubble-washed lettuce (BW) and 3.25 log CFU/g in micro-bubble-washed lettuce (MW). In comparison, total counts of samples examined after 10 days of storage were 7.00 log CFU/g for CT, 6.19 log CFU/g for HW, 6.02 log CFU/g for BW, and 5.89 log CFU/g for MW. The lowest counts were seen after micro-bubble treatment. BW and MW samples showed significantly higher counts than did CT and HW samples. In general, BW and MW samples did not vary significantly in count numbers. MW showed a 2.3-fold lower residual pesticide level compared with CT, and also had the lowest level of impurities. HW and BW samples were not well washed.