• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.369-375
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• 2001

Urushiol, a natural monomeric oil, was used to prepare a detergentless micro-emulsion with water and 2-propanol The formation of micro-emulsion was verified by conductivity measurements and dynamic light scattering. The conductivity data showed phase change dynamics, a characteristics of micro-emulsions, and subsequent dynamic light scattering study further confirmed the phenomenon. Average water droplet diameter was 10 nm to 500 nm when the molar ratio of 2-propanol ranged from 0.40 to 0.44 . Earlier studies were performed on toluene and hexane, in which the insoluble substrate in water phase was added to the solvents to be reacted on by enzymes. However, in the present urushiol system, urushiol was used as both solvent and substrate in the laccase polymerization of urushiol. The laccase activity in the system was examined using polymerization of urushiol. The laccase activity in the system was examined using syringaldezine as a substrate, and the activity increased rapidly near the molar ratio of 2-propanol at 0.4, where micro-emulsion started. The activity rose until 0.46 and fell dramatically thereafter. The study of laccase activity in differing mole fractions of 2-propanol showed the existence of an ‘optimal zone’, where the activity of laccase was significantly higher. In order to analyze urushiol polymerization by laccase, a bubble column reactor using a detergentless micro-emulsion system was constructed. Comparative study using other organic solvents systems were conducted and the 2-propanol system was shown to yield the highest polymerization level. The study of laccase activity at a differing mole fraction of 2-propanol showed the existence of an ‘optimal zone’ where the activity was significantly higher. Also, 3,000 cP viscosity was achieved in actual urushi processing, using only 1/100 level of laccase present in urushi.

• Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.115-117
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• 2006

A micro algal growth-enhancing polysaccharide compound was isolated from the green alga Monostroma nitidum using water extraction, molecular fractionation, a DEAE-cellulose column, and fast protein liquid chromatography using a Superose-12 column. The yield of the compound from the seaweed powder was 8.3$\times$l0$^{-3}$%. At 2 mg/mL concentration, the polysaccharide enhanced Tetraselmis suecica cell growth in f/2 medium by approximately 160%.

• International Journal of Concrete Structures and Materials
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• v.2 no.1
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• pp.63-68
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• 2008

High performance concrete (HPC) is widely used in civil engineering due to its high durability and low permeability etc. Compared with ordinary concrete, HPC may develop much higher AS (autogenous shrinkage) at early age due to the relative low water cement (w/c) ratio and adding of mineral admixtures, which is one of the main reasons for early age micro-cracking of HPC structures. This paper studies the early age property of HPC columns under similar construction-site surroundings by embedded strain transducers. Results show that for HPC structure, early-age autogenous shrinkage especially within the first day after concrete pouring is pretty large. AS within the first day are 60% larger than those for 14 days in this research for all specimens. Therefore it should be taken into account for structure durability. By comparison of PHPC (plain HPC column) and RHPC (reinforced HPC column) specimens, the effects of reinforced bars on AS and temperature distribution have been analyzed. Also the influence of w/c ratio on AS is demonstrated.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.12
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• pp.64-72
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• 2003

This paper presents a small loading and positioning device using VCM (voice coil motor). The developed device consists of a VCM-based linear actuating system, a capacitance displacement sensor and a cantilever deflection sensing system. The trust force of the VCM proportional to applied current moves the column supported on two pairs of parallel leaf springs. The infinitesimal displacement of moved column is detected by capacitance displacement sensor with a resolution of 0.1nm and a repeatability of 1nm. Also, a micro cantilever with known stiffness (200N/m), which is mounted on the end of the column, is used as a force sensor to detect the load applied to a specimen. After the cantilever contacts with the specimen, the deflection of cantilever and the load applied to the specimen are measured by using an optical lever system which consists of a diode laser, a mirror and a PSD (position sensitive detector). In this paper, an experimental system was constructed and its actuator and sensing parts were tested and calibrated. Also, the constructed system was applied to the indentation experiment and the load-displacement curve of aluminum was obtained. Experimental results showed that the developed device can be applied for performing nano indentation.

• Journal of Ocean Engineering and Technology
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• v.16 no.2
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• pp.72-79
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• 2002

In this study, time-dependent distributions of temperature and stresses, in the box-column welded from ultra thick plates with center segregation, has been analyzed by the commercial finite element package SYSWELD+, for several types and angles of groove. The major points of investigation are the optimum type and angle of groove that minimize weld stress specially at the center segregation, as well as temperature distribution, residual stresses and changes in the mechanical properties. The results can be summarized as follows; 1) Generally the thermal cycle at the root of groove exhibits relatively rapid cooling pattern, however, most of the other part weldment have a slow cooling pattern in all groove types. 2) Most of the micro-structures of weldment are composed of ferrite and pearlite, meanwhile we could find martensite and bainite locally a the root of the groove. 3) Optimum groove type for high heat input welding of box-column corner is a double groove type, and the optimum angle for the groove is 30~$45^{\circ}$ that minimize deformation and weld stress at the center segregation.

• Advances in concrete construction
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• v.9 no.3
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• pp.235-248
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• 2020

Progressive collapse in a structure occurs when load bearing members are failed and the adjoining structural elements cannot resist the redistributed forces and fails subsequently, that leads to complete collapse of structure. Recently, construction using precast concrete technology is adopted increasingly because it offers many advantages like faster construction, less requirement of skilled labours at site, reduced formwork and scaffolding, massive production with reduced amount of construction waste, better quality and better surface finishing as compared to conventional reinforced concrete construction. Connections are the critical elements for any precast structure, because in past, major collapse of precast structure took place because of connection failure. In this study, behavior of four different precast wet connections with U shaped reinforcement bars provided at different locations is evaluated. Reduced 1/3^rd scale precast beam column assemblies having two span beam and three columns with removed middle column are constructed and examined by performing experiments. The response of precast connections is compared with monolithic connection, under column removal scenario. The connection region of test specimens are filled by cast-in-place micro concrete with and without polypropylene fibers. Performance of specimen is evaluated on the basis of ultimate load carrying capacity, maximum deflection at the location of removed middle column, crack formation and failure propagation. Further, Finite element (FE) analysis is carried out for validation of experimental studies and understanding the performance of structural components. Monolithic and precast beam column assemblies are modeled using non-linear Finite Element (FE) analysis based software ABAQUS. Actual experimental conditions are simulated using appropriate boundary and loading conditions. Finite Element simulation results in terms of load versus deflection are compared with that of experimental study. The nonlinear FE analysis results shows good agreement with experimental results.

• Journal of the Earthquake Engineering Society of Korea
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• v.22 no.6
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• pp.353-360
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• 2018

This paper is to investigate the micro-behavior of the double-span beams with WUF-W seismic connection under combined axial tension and moment and to propose the rational rotational capacity of it for progressive collapse-resistant analysis and design addressing the stress and strain transfer mechanism. To this end, the behavior of the double-span beams under the column missing event is first investigated using the advanced nonlinear finite element analysis. The characteristics of fracture indices of double-span beams with WUF-W connection under combined axial tension and flexural moment are addressed and then proposed the rational rotational capacity as the basic datum for the progressive collapse-resistant design and analysis. The distribution of fracture indices related to stress and strain for the double-span beams is investigated based on a material and geometric nonlinear finite element analysis. Furthermore, the micro-behavior for earthquake and progressive collapse is explicitly different.

• Bulletin of the Korean Chemical Society
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• v.26 no.12
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• pp.1996-2000
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• 2005

Headspace hanging drop liquid phase micro-extraction (HS-HD-LPME) is studied as a novel solvent-based sample pretreatment method for floral volatile aroma compounds. This paper reports on application of the HSHD- LPME combined with GC-MS for the analysis of linalool component emitted from evening primrose flowers. The effect of several variables on the method performance was investigated. Additionally, the separation of enantiomers on a cyclodextrin capillary column was performed to identify chirality of (−)-linalool component. Since the unsurpassed volume of a few micro-liters of solvent is used, there is minimal waste or exposure to toxic organic solvents. This method enables to combine extraction, enrichment, clean-up, and sample introduction into a single step prior to the chromatographic process.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2005.03a
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• pp.413-417
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• 2005

A lattice model of a typical bridge column section is analyzed, and results are presented. The lattice is built as an ensemble of line elements and masses, that can capture strain rate dependency of concrete material. The research mainly breaks up into two parts: First, a micro level analysis of the material is executed, and control parameters of the governing equations are derived by matching the results with the common macroscopic properties of concrete material. Then, the properties exhibited by the micro model, which extends the classical material properties are applied to the mesoscale model. Hence, the analysis of the target structure can be performed. In the mesoscale analysis, ramp-like impulse loads are applied at different velocity, so that the contribution of the material level rate dependency to the global behavior of the structure can be tracked.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.29 no.6 s.237
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• pp.809-814
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• 2005

The goal of this paper is to minimize the weight of the T-structure supporting steering system in reducing the vibration level on steering wheel which could be amplified by the resonance. Presently, requirements for reducing noise, vibration and harshness (NVH) in automotive area are more stringent than ever. One of them is the vibration of steering system which occurs sometimes at high speeds or when the engine is idling. Besides, the reduction of weight is also one of requirements for improvement of vehicle performance. This paper used the micro genetic algorithm as an optimization method to satisfy above two requirements. The whole T-structure assembly including steering column was used for frequency analysis.