• Title/Summary/Keyword: micro cell counter

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Micro Cell Counter Integrated with An Oxygen Micropump (산소 미세 펌프가 내장된 미세 세포 계수기)

  • Son, Sang-Uk;Choi, Yo-Han;Lee, Seung-Seob
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.28 no.8 s.227
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    • pp.1159-1165
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    • 2004
  • This paper describes fabrication of a micro cell counter integrated with an oxygen micropump and counting experiment with Sephadex G-25 beads ($70{\sim}100\;{\mu}m$). The pumping part consisted of a microheater, catalyst (manganese dioxide) enveloped with paraffin, hydrogen peroxide, and microchannel, and the counting part consisted of collimated light, a microwindow, and a phototransistor including an external circuit. The micropump generated oxygen gas by decomposing hydrogen peroxide with manganese dioxide, which was initiated by melting the paraffin with the microheater, and pumped beads in the microchannel. When the beads passed the microwindow, they shaded the collimated light and changed the illumination on the phototransistor, which caused the current variation in the circuit. The signals, according to the bead size, reached up to 22 mV with noise level of 2 mV during 50 seconds and the numbers of peaks were analyzed by magnitude.

전기 저항법을 이용한 Micro Particle Counter Micro Fluidic Device 개발

  • Lee Jun;Yun Deok-Won;Chae Ho-Cheol;Han Chang-Su
    • Proceedings of the Korean Society Of Semiconductor Equipment Technology
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    • 2005.05a
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    • pp.134-138
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    • 2005
  • Recently many researches related with biotechnology are processed and it is the situation that research about micro fluidic devices is active. Micro fluidic devices has been one of the most widely used devices for the analysis in biotechnology because they have many advantages, flexibility, transparency, thermal and electrical stability, nontoxic, etc. In this study, micro fluidic device with PDMS is developed for particle counter which separates a small quantity of particles, The principle of micro particle counter is electrical-impedance method, and it was also applied hydrodynamic flow focusing. It is more efficient method to analyzing particles furthermore it can be applied to cell count ins for biotechnology.

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Micro Cell Counter Using a Fixed Control Volume Between Double Electrical Sensing Zones (다수의 계수구역간의 검사체적을 이용한 소형 세포농도센서)

  • Lee Dong Woo;Yi Soyeon;Cho Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.12 s.243
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    • pp.1615-1620
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    • 2005
  • We present a novel flow-rate independent cell counter using a fixed control volume between double electrical sensing zones. The previous device based on the single electrical cell sensing in a given flow-rate requires an accurate fluid volume measurement or precision flow rate control. The present cell counter, however, offers the flow-rate independent method for the cell concentration measurement with counting cells in a fixed control volume of $22.9{\pm}0.98{\mu}{\ell}$. In the experimental study, using the RBC (Red Blood Cell), we have compared the measured RBC concentrations from the fabricated devices with those from Hemacytometer. The previous and present devices show the maximum errors of $20.3\%\;and\;16.1\%$, which are in the measurement error range of Hemacytometer (about $20\%$). The present device also shows the flow-rate independent performance at the constant flow-rates ($5{\mu}{\ell}/min$ and $10{\mu}{\ell}/min$) and the varying flow-rate (4, 2, and $4{\mu}{\ell}/min$). Therefore, we demonstrate that the present cell counter is a simple and automated method for the cell concentration measurement without requiring an accurate fluid measurement and precision flow-rate control.

A Micro Cell Counter Integrated with Oxygen Micropump

  • Son, Sang-Uk;Choi, Yo-Han;Lee, Seung-S.
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2441-2444
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    • 2003
  • This paper describes fabrication of a micro cell counter integrated with an oxygen micropump and Sephadex G-25 beads counting experiment. The device utilized a phototransistor, microwindow, and light source of microscope for beads detection. Microheater and microchannel were used for pumping and guiding of beads to the microwindow. Counting capability of the device was tested with a peristaltic pump and the measured signals (${\sim}10\;mV$) with oscilloscope showed peak shape when beads passed the microwindow. Pumping of beads by the oxygen micropump was carried out by heating paraffin, which enveloped manganese dioxide (catalyst), to trigger the decomposition of hydrogen peroxide into water and oxygen. It lasted for 5 min with $7\;{\mu}l$ of wt. 30 % hydrogen peroxide. Beads counting by oxygen micropump showed peaks ($2{\sim}20\;mV$) with $30\;{\mu}l$ of beads sample and the number of peaks by magnitude was acquired.

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Effect of Transparency of CNT counter electrodes on the Efficiency of DSSCs

  • Lee, Won-Jae;Ramasamy, Easwaramoorthi;Lee, Dong-Yun;Song, Jae-Sung
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.07a
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    • pp.615-616
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    • 2005
  • Carbon Nanotubes (CNT) on flexible indium tin oxide (ITO) PET films were prepared for dye-sensitized solar cell (DSSC). These CNTs were prepared by spray coating method for various amount of light transparency. Also, Pt counter electrode was prepared by electro deposition method. All $TiO_2$ electrodes were deposited on ITO-PET films by spray coating method. Micro structural images show that CNT counter electrodes prepared by spray-coating have more dense structure with increasing spraying time (0 to 60 seconds). DSSC consisting of $TiO_2$ electrode and CNT counter electrode was fabricated with various amount of light absorption. DSSC have higher light energy conversion efficiency with increasing the thickness of CNT counter electrode. CNT counter electrode is at least compatible to that of CNT counter electrode.

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An Experimental Study on Heat Transfer Characteristics and Pressure Drop in Micro Plated Heat Exchangers with S-shape of Microchannel (S 형상의 마이크로 채널을 가진 마이크로 판형 열교환기의 열전달 특성 및 압력강하에 관한 실험적 연구)

  • Seo, Jang-Won;Kim, Yoon-Ho;Moon, Chung-Eun;Lee, Kyu-Jung
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.1876-1881
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    • 2007
  • The micro plated heat exchangers were designed to transfer more heat/volume or mass than previous heat exchangers within the context of the design constraints specified. The increase of the surface-to-volume ratio results in an increase of the interfacial area. This enhances considerably the performance of a heat exchanger. This can be an important component in a wide range of applications fuel cell, aerospace, automotive, electronic system and home heating, etc). In this study, the performance evaluation of micro plated heat exchangers under the counter flows with straight and S-shaped channel are carried out. The pressure drop as well as inlet and outlet fluid temperature were measured at steady state under various operating conditions and the total heat transfer rate were also calculated.

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Pulsed Electrochemical Deposition for 3D Micro Structuring

  • Park, Jung-Woo;Ryu, Shi-Hyoung;Chu, Chong-Nam
    • International Journal of Precision Engineering and Manufacturing
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    • v.6 no.4
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    • pp.49-54
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    • 2005
  • In this paper, micro structuring technique using localized electrochemical deposition (LECD) with ultra short pulses was investigated. Electric field in electrochemical cell was localized near the tool tip end region by applying pulses of a few hundreds of nano second duration, Pt-Ir tip was used as a counter electrode and copper was deposited on the copper substrate in mixed electrolyte of 0.5 M $CuSO_4$ and 0.5 M $H_2SO_4$, The effectiveness of this technique was verified by comparison with ECD using DC voltage. The deposition characteristics such as size, shape, surface, and structural density according to applied voltage and pulse duration were investigated. The proper condition was selected based on the results of the various experiments. Micro columns less than $10{\mu}m$ in diameter were fabricated using this technique. The real 3D micro structures such as micro spring and micro pattern were made by the presented method.

A Simulation Study of the Effect of Microstructural Design on the Performance of Solid Oxide Fuel Cells With Direct Internal Reforming (내부개질형 고체산화물 연료전지의 마이크로 전극구조가 성능에 미치는 영향에 관한 해석적 연구)

  • Sohn, Sangho;Nam, In Hyun
    • Transactions of the Korean hydrogen and new energy society
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    • v.24 no.5
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    • pp.401-412
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    • 2013
  • The paper is to study on the simulation of the micro/macroscale thermo-electrochemical model of a single cell of anode-supported SOFC with direct internal reforming. The coupled heat and mass transport, electrochemical and reforming reactions, and fluid flow were simultaneously simulated based on mass, energy, charge conservation. The micro/macroscale model first calculates the detailed electrochemical and direct internal reforming processes in porous electrodes based on the comprehensive microscale model and then solve the macroscale processes such as heat and mass transport, and fluid flow in SOFCs with assumption of fully-developed flow in gas channel. The simulation results evaluate the overall performance by analyzing distributions of mole fraction, current density, temperature and microstructural design in co/counter flow configurations.

Temperature measurement of the spray flame using micro scale absorption bands and line strength (마이크로 스케일의 흡수선과 흡수강도를 이용한 분무화염의 온도측정)

  • Choi, G.M.
    • Journal of ILASS-Korea
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    • v.7 no.2
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    • pp.1-6
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    • 2002
  • It is necessary to develope a high frequency diode laser sensor system based on the absorption spectroscopy for the measurement of temperature of the spray flame. DFB diode laser operating near $2.0{\mu}m$ was used to scan over selected $H_2O$ transitions near $1.9{\mu}m\;and\;2.2{\mu}m$, respectively. The measurement sensitivity at wide range of sweep frequency was evaluated using multi-pass cell containing $CO_2$ gas. This diode laser absorption sensor with high temporal resolution up to 10kHz was applied to measure the gas temperature in the spray flame region of liquid-gas 2-phase counter flow flame. The successful demonstration of time series temperature measurement in the spray flame gives us motivation of trying to establish non-intrusive temperature measurement method in the practical spray flame.

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Measurement of Linear Energy Spectra for 135 MeV/u Carbon Beams in HIMAC Using Prototype TEPC (프로토 타입 조직등가비례계수기의 중입자가속기연구소의 135 MeV/u 탄소 이온에 대한 선형에너지 스펙트럼 측정)

  • Nam, Uk-Won;Lee, Jaejin;Pyo, Jeonghyun;Park, Won-Kee;Moon, Bong-Kon;Lim, Chang Hwy;Moon, Myung Kook;Kitamure, Hisashi;Kobayashi, Shingo;Kim, Sunghwan
    • Journal of Sensor Science and Technology
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    • v.23 no.3
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    • pp.197-201
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    • 2014
  • TEPC (Tissue Equivalent Proportional Counter) was usually used for high LET radiation dosimetry. We developed a prototype TEPC for micro-dosimetry in the range of $0.2{\sim}300 keV/{\mu}m$. And, the simulated site diameter of the TEPC is $2{\mu}m$, of similar size to a cell nucleus. For purposes of characterization the response for high LET radiation of the TEPC has been investigated under 135MeV/u Carbon ions in HIMAC (Heavy Ion Medical Accelerator). We determined the gas multiplication factor and measured the lineal energy spectrum [yd(y)] of 135 MeV/u Carbon ions. The value of the gas multiplication factor was 315 at 700 V bias voltage. As a result of the experiment, we could more understand the performance of the TEPC for high LET (Linear Energy Transfer) radiation. And the procedure of high LET radiation dosimetry using TEPC is established.