• 약학회지
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• 제41권5호
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• pp.539-546
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• 1997

Capillary electrophoresis (CE) is perceived as an attractive tool for the analysis of pharmaceuticals and biological materials because of their high separation efficiency, easy separation and low running cost. New concept of micellar electrokinetic capillary chromatography (MECC) expanded the application of CE to the separation of neutral molecules. Validation of CE as an analytical technique for quality control of pharmaceuticals should be confirmed by quantitative analysis and the peak confirmation. In this study, the quantitative analyses of various types of neutral, acidic and basic components (acetaminophen, caffeine, ascorbic acid, riboflavin, thiamine, chlorpheniramine, phenylpropanolamine, dl-methylephedrine and dextromethorphan) in complex cold medicines have been accomplished using CE. Combined methods of MECC using SDS and capillary zone electrophoresis lowering the pH of running buffer were adopted to determine the ingredients in capsule type or liquid formula complex medicines without particular sample pretreatment. The results indicate that CE is a promising technique for quality control analysis of pharmaceuticals as a validation method.

• 분석과학
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• 제8권4호
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• pp.881-886
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• 1995

Several forms of gangliosides have been separated from various types of biological matrices using cyclodextrin-modified capillary zone electrophoresis (CZE). Quantitative analysis of phospholipids from biological fluids was achieved by micellar electrokinetic capillary chromatography (MECC) using 35mM sodium dodecyl sulfate. Phosphorylation, one of the most important post-translational modifications of proteins at serine, threonine and tyrosine residues in small peptides were identified and quantitative analyses of phosphopeptides were performed. Seven different neuropeptides which are relative the pain reachanism in the vertebrate central nervons system were also separated by CZE.

• 분석과학
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• 제19권1호
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• pp.50-57
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• 2006

계면활성제를 완충용액에 섞어서 사용하는 미셀 모세관 전기영동법(micellar electrokinetic capillary chromatography, MECC)을 이용하여, 아조염료의 합성성분이면서 동시에 아조염료가 분해될 때 생성되는 H-acid modifier 혹은 2-naphthylamine-1,5-disulfonic acid 등의 디아조 성분에 대한 분석을 수행하고, 이 분리 결과를 Ion-Paring 메카니즘을 이용한 고성능 액체 크로마토그래피법과 비교하였다. 이 방법을 이용하여 Direct Blue 2, Direct Blue 6, Direct Blue 15 등의 직접염료 그리고 Reactive Orange 4와 같은 반응성 염료의 혼합용액을 완전히 분리할 수 있었다. 특히 각 염료의 환원용액을 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 2-naphthylamine-1,5-disulfonic acid 등의 표준물질과 비교 분석한 결과, 사용한 각 염료의 디아조 성분인 H-acid modifier 혹은 2-naphthylamine-1,5-disulfonic acid에 대한 정보를 얻을 수 있었다. 본 연구의 결과를 토대로 Ion-Pair 크로마토그래피법과 모세관 전기영동법은 미지의 염료에 대한 성분확인 및 디아조 혹은 커플링 성분분석에 응용할 수 있음을 알 수 있었다.

• 분석과학
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• 제19권1호
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• pp.31-38
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• 2006

Ion-Paring을 이용한 고성능 액체 크로마토그래피법과 계면활성제를 완충용액에 섞어서 사용하는 미셀 모세관 전기영동법(micellar electrokinetic capillary chromatography, MECC)을 이용하여 아조염료의 합성성분이면서 동시에 독성을 나타내는 분해물인 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 그리고 2-naphthylamine-1,5-disulfonic acid 등의 디아조 성분에 대하여 분석을 수행하였다. 같은 방법으로 Acid Orange 7, Acid Orange 5, Acid Blue 92 등의 산성염료와 Direct Red 80 등의 직접염료와 같은 반응성 염료 및 Calcion에 대한 분리를 시도한 결과 모든 염료에 대한 완전한 분리를 얻었으며, 특히 각 염료의 환원용액을 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 혹은 2-naphthylamine-1,5-disulfonic acid 등의 표준물질과 비교 분석한 결과 사용한 각 염료의 디아조 혹은 커플링 성분을 완벽하게 분석할 수 있음을 알 수 있었고, 따라서 Ion-Pair 크로마토그래피법과 모세관 전기영동법은 미지의 염료에 대한 성분확인 및 디아조 혹은 커플링 성분분석에 응용할 수 있음을 보였다.

• Archives of Pharmacal Research
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• 제24권6호
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• pp.601-606
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• 2001

A simple assay method of recombinant human epidermal growth factor (rhEGF) in a pharmaceutical preparation was studied and validated by capillary electrophoresis (CE) using micellar electrokinetic chromatography (MEKC) techniques. Factors affecting the migration behavior and separation performances of the peptide; type of buffers pH, butler concentration, and concentration of sodium dodecyl sulfates (SDS) were investigated to optimize the analytical performance. CE was performed using running buffers 50.0 mM borate (pH 8.5) containing 12.5 mM SDS at 20 $mutextrm{V}$ of the applied voltage. Calibration curves for the rhEGF showed good linearity (r>0.999) over the wide dynamic range from 1.25 to $100{\mu\textrm{g}}/ml$. Sample analysis was performed by using standard addition method to eliminate the matrix effects of dosage vehicle. This method is assumed to be useful for quality control (QC) of various forms of pharmaceutical products of the peptide.