Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Study for the separation and comparison of azo dyes and their diazo components

아조염료와 디아조 성분의 분리 및 비교에 관한 연구

  • Jeong, Hyuk (Department of Chemistry, Sookmyung Women's University)
  • 정혁 (숙명여자대학교, 자연과학부, 화학전공)
  • Received : 2006.01.11
  • Accepted : 2006.02.10
  • Published : 2006.02.27
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Abstract

Well known environmental wastes from dye industry were separated by the micellar electrokinetic capillary chromatography(MECC). These wastes include H-acid modifier and 2-naphthylamine-1,5-disulfonic acid, and are known to be the diazo components of the azo dye. The results of the separation were compared with the result obtained by the HPLC using ion-pairing mechnism. MECC method was also applied to separate a few direct dyes including Direct Blue 2, Direct Blue 6 and Direct Blue 15, and reactive dye such as Reactive Orange 4. Informations about the diazo components of any azo dye could be obtained by comparison of electropherogram of the reduction solution of given dye with those obtained from standard materials such as H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid and 2-naphthylamine-1,5-disulfonic acid which are used as diazo components of the typical azo dyes. It has been concluded that MECC and HPLC with ion-pairing mechanism could be successfully applied for the analysis of unknown dyes and their diazo components.

계면활성제를 완충용액에 섞어서 사용하는 미셀 모세관 전기영동법(micellar electrokinetic capillary chromatography, MECC)을 이용하여, 아조염료의 합성성분이면서 동시에 아조염료가 분해될 때 생성되는 H-acid modifier 혹은 2-naphthylamine-1,5-disulfonic acid 등의 디아조 성분에 대한 분석을 수행하고, 이 분리 결과를 Ion-Paring 메카니즘을 이용한 고성능 액체 크로마토그래피법과 비교하였다. 이 방법을 이용하여 Direct Blue 2, Direct Blue 6, Direct Blue 15 등의 직접염료 그리고 Reactive Orange 4와 같은 반응성 염료의 혼합용액을 완전히 분리할 수 있었다. 특히 각 염료의 환원용액을 H-acid, J-acid, ${\gamma}$-acid, orthanilic acid, sulfanilic acid 2-naphthylamine-1,5-disulfonic acid 등의 표준물질과 비교 분석한 결과, 사용한 각 염료의 디아조 성분인 H-acid modifier 혹은 2-naphthylamine-1,5-disulfonic acid에 대한 정보를 얻을 수 있었다. 본 연구의 결과를 토대로 Ion-Pair 크로마토그래피법과 모세관 전기영동법은 미지의 염료에 대한 성분확인 및 디아조 혹은 커플링 성분분석에 응용할 수 있음을 알 수 있었다.

Keywords

References

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