• Title/Summary/Keyword: miR27a

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Fecal Microflora of Korean Neonates (한국인 신생아의 분변 미생물 균총)

  • Lee, Seung-Gyu;Jeong, Seok-Geun;Oh, Mi-Hwa;Kim, Dong-Hun;Kang, Dae-Kyung;Lee, Wan-Kyu;Ham, Jun-Sang
    • Journal of Dairy Science and Biotechnology
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    • v.27 no.2
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    • pp.1-6
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    • 2009
  • Probiotic bacteria have been administered to neonates to serve as maturational stimuli for the developing gut and intestinal immune system, establish and develop the intestinal microbiota, and mediate host-microbe interactions; further, these bacteria have shown beneficial effects In the treatment and reduction of the risk of infectious diseases, necrotizing enterocolitis, and atopic disease. An LAB isolation project to identify effective lactic acid bacteria for Korean people is in progress. The average total counts of lactic acid bacteria, lactobacilli, bifidobacteria, and coliforms in the fecal samples from 2 provinces were estimated as 8.31, 5.98, 8.13, and 3.01 CFU/g. Additional samples from other provinces will be analyzed to examine the changes in the lactic bacterial counts according to the area, sex of the neonate, mode of delivery, and type of feeding. A database containing the 16S rDNA sequences and the ribosomal protein profile of all the lactic acid bacteria isolated from fecal samples will be constructed. For the effective use of probiotics, a number of clinical studies are needed to formulate guidelines for strain, subject, purpose, and dose.

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Antioxidant Activities and Antioxidant Compounds of Some Specialty Rices (여러 가지 특수미의 항산화 활성 및 항산화 성분)

  • Seo, Sun-Jung;Choi, Young-Min;Lee, Seon-Mi;Kong, Su-Hyun;Lee, Jun-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.2
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    • pp.129-135
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    • 2008
  • The objectives of this study were to determine antioxidant activity of the methanolic extracts from some specialty rices and to investigate relationships between antioxidant activities and antioxidant contents in the extracts. ABTS (2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) radical cation scavenging activity, inhibitory effect on lipid peroxidation, chelating activity, reducing power and inhibitory effect on xanthine oxidase have been used to investigate the relative antioxidant activity of the extracts from specialty rices. The concentrations of total polyphenolics, phytic acid, and anthocyanin in the extracts were measured by spectrophotometric methods and vitamin E analysis was carried out by HPLC. The methanolic extracts prepared from black and red rices showed higher antioxidant activities and contained higher antioxidant compounds compared with other rices, apparently due to their intense red-purple color. The correlation coefficient between total polyphenolic content of methanolic extracts and ABTS radical cation scavenging activity, reducing power, and inhibitory effect of xanthine oxidase were 0.9921, 0.9856, and 0.8032, respectively.

Detection Method for Identification of Pueraria mirifica (Thai kudzu) in Processed Foods (가공식품 중 태국칡(Pueraria mirifica) 혼입 판별법 개발)

  • Park, Yong-Chjun;Jin, Sang-Wook;Kim, Mi-Ra;Kim, Kyu-Heon;Lee, Jae-Hwang;Cho, Tae-Yong;Lee, Hwa-Jung;Lee, Sang-Jae;Han, Sang-Bae
    • Journal of Food Hygiene and Safety
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    • v.27 no.4
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    • pp.466-472
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    • 2012
  • In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls.

The Motivation of selecting major, the satisfaction of major and view of occupation for EMT students (응급구조(학)과 학생의 전공선택 동기와 전공만족도 및 직업관)

  • Kim, Mi-Sook;Park, So-Mi;Wang, Chengying;Seo, Ha-Yan;Joo, Young-Ju;Lee, Kyoung-Youl;Choi, Eun-Sook
    • The Korean Journal of Emergency Medical Services
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    • v.14 no.3
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    • pp.29-40
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    • 2010
  • Purpose: We investigated the motivation of selecting major, the satisfaction of major and the view on occupation of emergency medical technology (EMT) students. The results of study will be used to increase the satisfaction of major of university students. Also, it can be used to support decision of major for high school students. Method: We conducted 1,586 surveys from 665 students at six four-year colleges and 921 students at seven three-year colleges. Our research period was from Aug. 27th to Oct. 31st in 2010. The surveys were analyzed by SPSS 18.0 using description statistics, t-test, ANOVA, Scheffe and Pearson correlation coefficient. Result: In our study, the motivation of selecting major is 35.8% of students selected 'good employment prospects', satisfaction of major's $M{\pm}SD$ is $3.15{\pm}.486$, adaptation of major's $M{\pm}SD$ is $3.11{\pm}.472$. The satisfaction of major show difference (t = 4.548 p = .000) by sex, also the adaptation of major show difference (t = 2.279, p = .023) by sex. The satisfaction of major show first grade students higher (F = 3.605, p = .013) than fourth grade students at four-year colleges. If satisfaction of major is high, accumulation evaluation score is high (F = 3.276, p = .011), too. Clinical practice experienced students was higher (t = -2.878, p = .004) than non experienced it satisfaction. In view of occupation, ideal job's factors and actual job's factors a lot of students selected 'aptitude'. Also, there is a statistically significant correlation (r = .618, p = .000) between the satisfaction of major and the adaptation of major. High satisfaction indicates high adaptation of major. Conclusion: In our study satisfaction of major and adaptation of major was very high score. EMT students concern about employment prospects at most. It is inferred that they select job which match with one's aptitude. We can suppose that students select major as a tool for employment by seeing result that a lot of students consider employment prospects at most when they select major. A method to improve the satisfaction and adaptation of major should be developed by realizing problems which occur the dissatisfaction of major. Also, there as on why students conflict between ideal and actual job should be revealed. There as on seems students want stable occupation in unstable job market situation. Therefore, the expansion of job matching aptitude and being stable should be processed. Finally, university should actively support the method that help to finding jobs for student by identifying job preparation of students.

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Effect of Plant Growth Regulators on Regeneration from the Cotyledon Explants in Watermelon (Citrullus lanatus (thunb.) Matsum. & Nakai) (수박(Citrullus lanatus (thunb.) Matsum. & Nakai) 자엽 절편의 재분화에 미치는 생장조절물질의 영향)

  • Cho, Song Mi;Oh, Sang A;Choi, Yong Soo;Park, Sang Bin
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.51-59
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    • 2014
  • In this study, we developed a high frequency watermelon regeneration system using three breeding lines ('B02', 'B05' and 'D04') of watermelon (Citrullus lanatus (thunb.) Matsum. & Nakai) which are differed in their fruits in shape, color of pericarp and flesh. The highest frequency of explants with callus was observed by using explants that consist of cotyledon proximal part end in all breeding lines, and the highest rate of callus induction was obtained on MS medium containing 1.0 mg/L BAP + 0.5 mg/L IAA for 'B02' (94%), 3.0 mg/L BAP for 'B05' (95%), 3.0 mg/L BAP + 0.1 mg/L IAA for 'D04' (90%). The highest shoot regeneration rates from derived callus were obtained on MS medium containing 1.0 mg/L BAP + 0.5 mg/L IAA for 'B05' (94%), and then a 'B02' (81%) with a same culture conditions, and the lowest regeneration was obtained on MS medium containing 1.0 mg/L BAP for 'D04' (56%). Regenerated plants showed the best rates of root formation on MS containing 0.1 mg/L IBA for 'B02' (67%), 0.1 mg/L NAA for 'B05' (87%), 0.5 mg/L IAA for 'D04' (88%). The regenerated plants showed a 100% survival rate in soil condition. The tissue culture and regeneration conditions obtained from this study will be useful for regenerating plants in breeding applications, and will be a useful tool for further genetic transformation studies on watermelons.

Geographical origin discrimination of Korean variety, Angelica gigas Nakai by using TDU (Thermal Desorption Unit)-GC/MS (TDU-GC/MS를 이용한 한국품종 참당귀 국산 판별 기법)

  • Lee, Mi-Na;Kim, Yoon-Suk;Kim, Won-Il;Kim, Jeong-Gyu;Kwon, Oh-Kyung
    • Journal of Applied Biological Chemistry
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    • v.63 no.1
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    • pp.29-33
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    • 2020
  • The objective of this study was to develop a fast and accurate method of variety discrimination and geographical discrimination origin of Korean angelica (Korean variety, Angelica gigas Nakai) by using TDU-GC/MS. Two peaks of decursin and decursinol, which are coumarin derivatives were identified in the range of Total Ion Chromatogram (TIC) RT 26.9-27.2 of the Korean angelica by GC/MS analysis at the time of condensation in a refrigerated condensation system after thermal desorption of sample extracts. In case of Chinese angelica (Chinese variety), ligustilide peak was detected at the RT 17.2. In order to investigate the difference of volatile components according to the geographical origin of Korean variety, the mass spectra were measured by TDU-GC/MS at the range of m/z 40-400 amu. The TIC of domestic cultivation and Chinese cultivation of the Korean variety, Angelica gigas Nakai showed the same tendency as a whole. However, in partial scans of TIC, two peaks detected at 15.54 and 16.05 of RT showed different peak patterns between Korean angelica (Korean variety) cultivated in Korea and in China. The ratio of Peak A (RT 15.54) and B (RT 16.05) was 0.0-0.2 for domestic cultivation and 0.5-2.8 for Chinese cultivation, confirming the possibility of discriminating origin by comparing the TIC peak pattern of TDU-GC/MS.

Leg Length Inequality, Habitual Posture, and Pain in Women's College Students (여대생의 다리길이 차이와 생활습관 자세 및 통증)

  • Kwon, Sung-Bok;Yi, Yeo-Jin;Han, Hye-Ja;Cho, Kyung-Sook;Lim, Nan-Young;Lee, Eun-Hee;Son, Haeng-Mi;Park, Young-Sook;Kim, Joo-Hyun;Kang, Hyun-Sook
    • Journal of muscle and joint health
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    • v.19 no.1
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    • pp.27-36
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    • 2012
  • Purpose: The purpose of this study was to investigate the leg length Inequality, habitual posture, and pain in women's college students. Methods: The subjects were 281 students, in 8 women's college in Korea. The tapelines were used for measuring leg length Inequality and questionnaires were used for measuring habitual posture, and pain. The data were collected between August and October 2010 and analyzed using SPSSWIN 11.5. length Inequality(<1 cm). The subjects of 2.8% were the length differences of above 2cm. The worst habitual posture were leaning habits. Especially, the habits of crossing her legs were significantly different to leg length Inequality(F=3.342, $p$=.037). The subjects of 84% were felt a severe pain on the upper body such as waist, back, scapula, neck or shoulder. But there is no difference between pain and leg length Inequality. Habitual posture are related to pain(r=.212, $p$=.001). Conclusion: To protect the severe health problem of musculoskeletal system, this study results will be give aid to health education in women's college students.

A Study on Risk Factors of Musculoskeletal Disorders among Selected Female Hair Dressers (일부 여성 미용종사자들의 근골격계 증상의 위험요인)

  • Song, Mi-Ra;Han, Sung-Hyun
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.15 no.3
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    • pp.250-260
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    • 2005
  • This study was designed to find the symptom prevalence rates of musculoskeletal disorders and the risk factors of musculoskeletal disorders among selected hair dressers. Methods; The survey were conducted from July 1 to 30, 2004 for 459 hair dressers working in Seoul and Gwangju using self-administration questionnaire. The risk factors on musculoskeletal disorders have analysed by multiple linear regression analysis. The results of this study are summarized as follows: The experience rate of musculoskeletal disorders symptom in the last one year was 56.4%. The experience rates of each body parts were 36.4% on the shoulders, 30.5% on the legs and the feet, 28.5% on the low back, 27.0% on the hand and the fingers, 23.3% on the neck and 17.0% on the arms and the elbows. The prevalence rate of musculoskeletal disorders symptom in the last one week was 40.5%, those of each body parts were 24.0% on the legs and the feet, 21.4% on the shoulders, 20.7% on the low back, 15.7% on the hand and the wrists, 15.0% on the neck, 9.4% on the arms. The risk factors on musculoskeletal disorders were working posture(${\beta}=0.32$), perceived stress(${\beta}=0.19$), working period(${\beta}=0.16$), standing working over 10 hour(${\beta}=0.16$), hard working(${\beta}=0.11$). The adjusted determinant coefficient($R^2$) of this regression model was 0.267. Based on the results, in order to prevent musculoskeletal disorders of hair dressers, working posture shall be improved and leisure opportunities to relieve stress, and health management program shall be provided.

Analysis on the Donators' Characteristics of Government-dominant and Non-governmental Food Bank Programs (정부주도형 및 민간주도형 푸드뱅크(Food Bank) 사업의 기탁 특성 분석)

  • Jo, Mi-Na;Hong, Min-Ah;Kang, Hye-Seung;Yang, Il-Sun
    • Korean Journal of Community Nutrition
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    • v.11 no.5
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    • pp.618-628
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    • 2006
  • The purpose of this study was to examine the donators' characteristics and donative foods of both government-dominant and non-governmental food bank program, to understand the problems and benefits of food bank program, and to find the solutions to activate food bank program. The questionnaires were distributed to 120 food bank operators and 3 donators were selected from each food bank for the survey from April 2002 to May 2002. 118 sheets (32.8%) from government-dominant food bank and 53 sheets (20.1%) from non-governmental food bank were collected. The main results of this study were as follows: The largest donators to either government-dominant or non-governmental food bank programs were bakery and confectionery companies 31.4% and 45.3% respectively. The majority of donated foods were 'goods in stock' (55.6%) and frequency of donation were largely on a daily basis (27.1% for government-dominant and 22.6% for non-governmental). Some of the donators who had more donative food did not donate, and the reasons were closeness to expiration date of food (67.3%), lack of legal protection in the event of food poisoning (54.5%), and poor public image of food related accidents (52.3%).

Ethical Dilemma and Coping Types in Nurses (간호사의 윤리적 딜레마와 대처유형)

  • Park, Hyun-Ju;Kim, Mi-Ye
    • The Journal of Korean Academic Society of Nursing Education
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    • v.10 no.1
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    • pp.75-83
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    • 2004
  • This study was conducted to examine the ethical dilemma and coping types of nurses, and identify the relationship between ethical dilemma and coping types of nurses. The subjects of this study consisted of 210 nurses from two university hospitals in Taegu and one university hospital and one general hospital in Pohang. The data were collected with self reported questionnaire from June 2003 to July 2003. The data were analysed using the SPSS program. The results of this study were as follows : 1. The mean score of level of ethical dilemma was 0.55/1, and client area was the highest(M=0.65). 2. The mean score of level of coping about ethical dilemma was 2.22/4, and seeking social support area was the highest (M=2.42). 3. The conflict of proffering the best care with courtesy to impolite patient was the highest in total items of ethical dilemma. Criticized or lectured myself was the highest in total items of coping type. 4. In general characteristics, age(F=4.74, p<.01), marital status(t=2.67, p<.01), career (F=5.95, p<.01) were significantly related to level of ethical dilemma and religion(t=2.27, p<.05) was significantly related to types of coping. There was positive correlation between ethical dilemma and types of coping(r=.228, p<.01). On the basis of these findings, it is needed a correlation study according to category of ethical dilemma and coping types of nurses, and to develop the coping strategy that resolves ethical dilemma in nursing practice.

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