• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1511-1515
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• 2014

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

• Research in Community and Public Health Nursing
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• v.17 no.1
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• pp.125-133
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• 2006

Purpose: This study was performed to identify the relationship between PMS and sex role. Method: Data were collected from 417 female college students in Seoul and Gyeonggi-do area during the period from the 6th of September to the 15th of October in 2004. Collected data were analyzed using SPSS WIN 11.0. Result: The results of this study were as follows: 1) Perceived subjective symptoms were clustered endogenous depressive feature ($2.76{\pm}.97$), anxiety ($2.76{\pm}1.09$), and impulsivity ($2.72{\pm}1.02$). 2) PMS was significantly different according to the general characteristics of menarche age (F=5.910, p=.003), pain (F=7.886. p=.000) and family history (F=5.366, p=.005), masculinity was significantly different according to menarche age (F=3.174, p=.043), and femininity was significantly different according to menarche age (F=3.742, p=.025) and pain (F=3.256. p=.040). 3) Femininity and PMS were in a positive correlation with each other (r=.623. p=.000). Conclusion: This results showed that there is a significant correlation between PMS and sex role. For future research, it is recommended to identify major factors affecting PMS and the relationships between them and various subjects.

• The Journal of Korean Academic Society of Nursing Education
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• v.12 no.1
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• pp.43-51
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• 2006

Purpose: This study was performed to identity the relationship between PMS and menstrual attitudes. Method: Data were collected from 417 female college students in Seoul and Gyeonggi-do area during the period from the 6th of September to the 15th of October in 2004. Collected data were analysed using the SPSS WIN 11.0. Result: The results of this study were as follows: 1) Perceived subjective symptoms were clustered endogenous depressive feature($2.76{\pm}.97$), anxiety($2.76{\pm}1.09$), and impulsivity($2.72{\pm}1.02$). 2) PMS was significantly different according to the general characteristics of grade(F=3.414, p=.018), major(F=2.755, P=.004), menarche age(F=5.910, p=.003), pain(F=7.886, p=.000), family history(F=5.366, p=.005). 3) Menstrual attitudes was significantly different according to the general characteristics of grade(F=3.315, p=.020), major(F=3.813, P=.000), religion(F=6.313, p=.000), interval of menstruation(F=2.834, p=.016), pain(F=3.059, p=.048), family history(F=3.062, p=.048). 4) Menstrual attitudes and PMS were in a positive correlation with each other (r=.112, p=.002). Conclusion: This results showed that there is a significant correlation between PMS and menstrual attitudes. For further research, it is recommended to identity major factors affecting PMS and the relationships between them and various subjects.