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http://dx.doi.org/10.7314/APJCP.2014.15.4.1511

MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells  

Zhang, Lei-Lei (Department of General Pathology, Huaihe Hospital, Henan University)
Wu, Jiang (Department of General Pathology, Huaihe Hospital, Henan University)
Liu, Qiang (Department of General Pathology, Huaihe Hospital, Henan University)
Zhang, Yan (Department of General Pathology, Huaihe Hospital, Henan University)
Sun, Zhu-Lei (Department of General Pathology, Huaihe Hospital, Henan University)
Jing, Hong (Department of General Pathology, Huaihe Hospital, Henan University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.4, 2014 , pp. 1511-1515 More about this Journal
Abstract
Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.
Keywords
MCF-7; miR-886-5p; caspase; MT1-MMP; MMP2;
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