• Bulletin of the Korean Chemical Society
• /
• v.30 no.11
• /
• pp.2655-2660
• /
• 2009

A breed of cattle, i.e., Korean cattle (Hanwoo), was identified based on the DNA mobilities of their microsatellites (MSs) by capillary gel electrophoresis (CGE) with a laser-induced fluorescence (LIF) detector. The MS markers were used for the accurate identification of species-specific genes. The DNA mobilities of the MS markers of Hanwoo and Holstein were measured using a CGE system with a fused-silica capillary (inner diameter of 75 ${\mu}m$, outer diameter of 365 ${\mu}m$, and total length of 50 cm). The capillary was dynamically coated with 1.0% (w/v) polyvinylpyrrolidone ($M_r$ = 1,000,000) and then filled with a mixture of 1.3% (w/v) poly(ethylene oxide) ($M_r$ = 600,000) and 1.9% (w/v) poly(ethylene oxide) (Mr = 8,000,000) as a sieving gel matrix. The species-specific genes of Hanwoo and Holstein were clearly distinguished within 33 min. This CGE assay technique is expected to be a useful analytical method for the fast and accurate identification of breeds of cattle.

• Journal of Korean Clinical Nursing Research
• /
• v.16 no.3
• /
• pp.51-61
• /
• 2010

Purpose: The purpose of this study was to determine the effect of needs for professional development and organizational climate on organizational socialization of clinical nurses. A cross-sectional analysis were performed to assess the factors affecting organizational socialization. Methods: The data used in this study were obtained from clinical nurses who were employed in a hospital (N=606). Using multiple regression, we tested variables to assess their effects on organizational socialization in this sample. The data were analyzed using descriptive test, t-test, ANOVA, Pearson correlation coefficiency and stepwise multivariate regression. SPSS 17.0 program was utilized for data analysis. Results: The mean scores of organizational socialization, needs for professional development and organizational climate were statistically differed by career ladder, educational level and position. Organizational socialization had significant positive correlations with the needs for professional development (r=.332, p<.01) and organizational climate (r=.523, p<.01). Those variables including career ladder explained 33.4% of organizational socialization. Conclusion: Our findings indicate that organizational socialization of clinical nurses could be enhanced by meeting the needs for professional development and organizational climate. Developing innovative educations for encouraging clinical nurses' carrier development and creating a positive organizational climate are mandated for clinical nurses to have constructive organizational socialization.

• Journal of Digital Convergence
• /
• v.16 no.8
• /
• pp.253-261
• /
• 2018

The purpose of this study was to examine the predictive relationships between the predictors and self-esteem among hemodialysis(HD) patients. Predictive correlational design, participants 154 HD patients in the Seoul. SPSS for Window 22.0 used to perform descriptive statistics, Pearson's Correlation Coefficient and Stepwise Regression analysis. The strongest predictor was uncertainty (${\beta}=-.33$, p<.001), economy (${\beta}=.18$, p<.05), occupation (${\beta}=.17$, p<.05) and health status (${\beta}=.16$, p<.05) were followed. A total explained variance was 30.0% of self-esteem (Adjust $R^2=.30$). Managing or preventing uncertainty, providing vocational education in consideration of the dialysis environment, providing employment, increasing economic level, and maintaining good health status are factors that enhance self-esteem. Conducted to investigate the self-esteem of HD patients considering the machine-dependent characteristics, future, provide the data of the study to improve the self-esteem of HD patients. Also, nursing intervention should be developed based on the findings.

• Restorative Dentistry and Endodontics
• /
• v.27 no.1
• /
• pp.54-65
• /
• 2002

Black-pigmented bacteria have been implicated in the endodontic infections. This group of microorganisms includes Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, and Prevotella nigrescens. The organisms display a wide variety of virulence factors that may be pertinent to acute endodontic infections. The aim of this study was to identify P. endodontalis, P. gingivalis, P. intermedia, and P. nigrescens by using special potency disk test, filter paper spot test, 165 rRNA gene-directed PCR, and API 32A. Microbial samples were collected from root canals of 33 intact teeth with necrotic pulp and/or apical periodontitis. Conventional laboratory methods were used for identification of the strains of black pigmented bacteria. Eighteen of 33 samples were positive for the growth of black-pigmented bacteria Five colonies were cultured from each pure cultured colonies from Brucella agar plate. Seventy seven colonies were positive for the growth of black-pigmented bacteria. Thirty three of 77(42.6%) were identifed as P. nigrescens, 10 of 77(12.9%)were P. gingivalis, 6 of 77(7.8%) were P. endodontalis, 10 of 77(12.9%) were P. intermedia. On the contrary the reference strains of P. nigrescens, experimental strains of P nigrescens was sensitive to kanamycin in special potency disk test. 165 rRNA gene PCR and API test after rapid presumptative identification methods, such as special potency disk test and filter paper spot test, would be accurate detection methods for black-pigemented bacteria.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.33 no.4
• /
• pp.624-632
• /
• 2006

In recent years, xenon plasma arc lamp was introduced for high-intensity curing of composite filling materials in direct resin restorations. In this study, two types of restorative materials, namely composites point $4^{(R)}$ and $Z250^{(R)}$ were selected and curing was conducted using a conventional halogen light and two plama curing lights. Two different resin composites were cured using the different units($Flipo^{(R)}$, Ultra-lite 180A, and $TriLight^{(R)}$) and tested for microhardness. The purpose of this study was to test the hypothesis that exposure to a plasma curing lamp for 3, 6. 9 seconds is equivalent to 20 or 40 seconds of irradiation using a conventional halogen curing unit. 1. $Flipo^{(R)}$ and Ultra-lite 180A were able to polymerize point $4^{(R)}$ at 6 seconds to a degree equal to that of the $TriLight^{(R)}$(control) at 40 seconds. 2. $Flipo^{(R)}$ was able to polymerize $Z250^{(R)}$ at 9 seconds to a degree equal to that of the $TriLight^{(R)}$(control) on the bottom surface at 20 seconds. whereas Ultra-lite 180A could not do. 3. Two plasma curing units were able to cure the test-composites with bottom/top ratios approximately 61% to 96% at 3 to 9 seconds. There were some differences between the two composite brands, with $Z250^{(R)}$ displaying less difference between top and bottom hardness values. For point $4^{(R)}$ and $Z250^{(R)}$, at least 6 or 9 seconds were necessary to produce microhardness equivalent to that of the $TriLight^{(R)}$ curing at 20 or 40 seconds.

• Communications for Statistical Applications and Methods
• /
• v.13 no.3
• /
• pp.733-743
• /
• 2006

To apply and evaluate the effectiveness of genetic markers on Hanwoo traceability systems, samples of 33 Hanwoo individuals from Korean elite sire families were used, and five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Ten major microsatellite markers were selected from alleles amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

• 한국데이터정보과학회:학술대회논문집
• /
• 2006.04a
• /
• pp.213-219
• /
• 2006

Considering all the factors involved in beef production individual identification using DNA marker testing is the most appropriate solution to give all the breeders' information to the consumers. After taking into account the genealogical information from the Hanwoo, only animals that did not share some parent or grandparent were analysed 33 from the 305 initially sampled. Ten major microsatellite markers were selected from allele amplified and their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

• 한국데이터정보과학회:학술대회논문집
• /
• 2006.11a
• /
• pp.155-159
• /
• 2006

Samples of 33 Hanwoo individuals from Korean elite sire families were used and five microsatellite markers were selected finally, which were located on chromosomes different chromosomes with the end sequencing of 100 HW-YUBAC that were recorded in the NCBI by Yeungnam University. Ten major microsatellite markers were selected from alleles amplified, their frequencies, H(Heterozygosity) and PIC(Polymorphism information content) with Hardy-Weinberg equilibrium. Next, in order to evaluate the Power of the markers selected on the individual animal identification, the match probability(MP) and the relatedness coefficient(R) were computed.

• Journal of Oriental Neuropsychiatry
• /
• v.23 no.3
• /
• pp.23-32
• /
• 2012

Objectives : The purpose of this study was to validate the Sasang Personality Questionnaire (SPQ), based on the diagnosis of Sasang constitutional medicine clinical specialist. Methods : We performed Sasang type differentiation of 103 college students by certified specialist, and analyzed biopsychological traits of each groups with SPQ, Temperament and Character Inventory (TCI), Positive and Negative Affection Schedule (PANAS) and Body Mass Index (BMI). Results : The SPQ score of So-Yang ($33.1{\pm}0.79$), Tae-Eum ($29.03{\pm}0.67$) and So-Eum ($25.76{\pm}0.98$) Sasang groups showed significant differences from each other. The SPQ showed significant correlation with TCI Novelty-Seeking (r=.346), TCI Harm-Avoidance (r=-.348) and TCI Persistence (r=.346). But the BMI and PANAS did not show significant correlation with SPQ, although the PANAS Positive Affection (r=.332) has significant correlation with SPQ-Behavior. Conclusions : We confirmed the clinical validity of SPQ with clinical specialist and established biopsychosocial instruments. This study would contribute to a more objective diagnosis and studies on biopsychosocial mechanism of Sasang constitutional medicine.

• Journal of the Korea Convergence Society
• /
• v.9 no.2
• /
• pp.33-42
• /
• 2018

The purpose of this study is to identify the degree of dating violence experience, violence tolerance, and social problem solving ability of nursing college students and to investigate the relationship between them. 219 college students from nursing in 5 universities of Chungcheong region were convenience selected for the study. Data were collected through self-report questionnaires. Data were analyzed by t-test, ${\chi}^2$ test and Partial correlation coefficients using the SPSS 20.0 program. As a result, nursing college student's the more experience dating violence, the higher the tolerance for dating violence(r=0.31, p<.001), the higher the tolerance of dating violence is, the more negative the ability to solve social problems(r=-0.26, p<.001), and the degree of their departmental satisfaction and drinking are related to the violence of dating violence. Therefore, it provided a rational basis for the establishment of a dating violence preventive education and training program to raise awareness of dating violence tolerance and social problem solving abilities in order to prevent dating violence.