• 제목/요약/키워드: miR-33

검색결과 255건 처리시간 0.031초

한국의 멸종위기종인 꼬마잠자리(Nannophya pygmaea Rambur: 잠자리과, 잠자리목) 알의 발육과 온도의 관계 (Relationship between Temperature and Egg Development of Nannophya pygmaea Rambur (Odonata: Libellulidae), an Endangered Dragonfly in Korea)

  • 김동건;황정미;윤태중;배연재
    • 환경생물
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    • 제27권3호
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    • pp.292-296
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    • 2009
  • 본 연구는 한국의 멸종위기곤충인 꼬마잠자리 알의 발육과 온도의 관계를 추정하고자 8가지 서로 다른 온도 조건(17, 20, 22, 25, 28, 30, 33 및 $36^{\circ}C$)에서 실시하였다. 꼬마잠자리의 알은 2007년 6월 경상북도 문경시 일대의 산간 습지에 서식하는 암컷 성충으로부터 채취하였다. 실험의 결과, 부화율은 17, 20, 22, 25, 28, 30, 33, 그리고 $36^{\circ}C$에서 각각 2.86, 17.09, 24.32, 39.67, 34.43, 40.57, 44.79 및 1.75%였다. 각 온도에서의 알의 발달율(평균발육속도)은 변형된 Sharpe와 DeMichele의 비선형 모형에 부합하였다. 알의 저온발육임계온도는 $14.02^{\circ}C$(y=0.005988x-0.084, $r^2$=0.99)로 추정되었고, 발육최적온도는 $30{\sim}35^{\circ}C$로 추정되었다.

마사지 요법과 운동요법이 비만여성의 신체조성과 혈중 콜레스테롤에 미치는 영향 (The Effect of Massage Therapy and Exercise Therapy on Body Composition and Blood Cholesterol in the Obese women)

  • 이미선;한미정;이영선
    • 한국패션뷰티학회지
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    • 제2권1호
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    • pp.93-102
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    • 2004
  • The purpose of this study is to examine the effect of massage therapy and exercise therapy on body composition and blood cholesterol in the obese women. Sixteen college women were randomly assigned to aged $20.1{\pm}2.7$ years who had not exercised regurlarly, volunteered to participate in the present study as subjects. They completed a 12 weeks massage therapy and exercise therapy performed 3 times/week, for 60min/session. Massage put in exercise treatment effect of 12 weeks in BMI has shown a significant decrease those are before test $25.1{\pm}2.2$ to after test $23.7{\pm}3.2$(p<0.05). The waist measure/height has a difference by before test r=0.5 to after a test r=0.47, and waist measure/hips measure by before test r=0.8 to after test r=0.75. A total cholesterol(TC) average value has $187.6{\pm}36.9$ before test to $168{\pm}32.4$(p<0.05) after test, a significant difference. Also average value of LDLC has $114{\pm}33.8$ before test to $99.2{\pm}30.4$(p<0.05) that shows a difference, but HDLC was $52{\pm}7.5$ before test to $52.3{\pm}7.7$(p<0.05) after test which means a none difference. HDLC does not have any difference but total cholesterol and LDLC were significantly different. It was a short program of 3 times a week, 60 min/session but, a dance meditation exercise program with high strength self massage had given a big effect in consideration. Using these scientific studies data, wish a program related by obese could make an activation in a beauty part.

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The impact of freeze-drying on the glycoproteomic profiles of human milk

  • Hahn, Won-Ho;Bae, Seong-Phil;Lee, Hookeun;Park, Jong-Moon;Park, Suyeon;Lee, Joohyun;Kang, Nam Mi
    • 분석과학
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    • 제33권4호
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    • pp.177-185
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    • 2020
  • Human milk (HM) glycoproteins play important roles protecting infants against various pathogens. Recently, freezing HM is reported to affect some glycoproteins and freeze-drying is suggested as an alternative method. However, the effects of freeze-drying on HM glycoproteins were not evaluated yet. Six fresh HM samples were collected from three healthy mothers at 15 and 60th days of lactation from each mother. Each sample was divided into frozen and freeze-dried subgroups yielding totally 12 samples, and the glycoproteomic analysis was performed by liquid chromatography mass spectrometry. The results were compared between samples of 15 and 60th days of lactation, and before and after the freeze-drying. Totally, 203 glycoproteins were detected. The glycoprotein levels were not different between two groups of 15/60th day of lactation and before/after freeze-drying groups (P > 0.050). In addition, significant correlation of glycoprotein levels was found between the different lactation stages (r = 0.897, P < 0.001) and the status of freeze-drying (r = 0.887, P < 0.001) in a partial correlation analysis. As no significant change of HM glycoproteins was not found after the freeze-drying, we hope that introducing freeze-drying to HM banks is supported by the present study. This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Korea government (MSIP) (No.2017R1D1A1B03034270; No.2020R1A2C1005082).

황기추출물의 항노화 효과 (Anti-aging Effect of Astradalus membranaceus Root Extract)

  • 윤경섭;김미진;임경란;정택규
    • 대한화장품학회지
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    • 제33권1호
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    • pp.33-40
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    • 2007
  • 예로부터 황기는 민간에서 강장제로서 인삼 다음의 보약으로 쓰이고 있다. 본 연구에서는 황기의 성분 중 플라보노이드 화합물로 알려진 calycosin과 formononetin을 유효성분으로 함유하는 황기추출물의 항노화 효과에 대해 알아보았다. Calycosin과 formononetin의 함량은 효소처리 추출방법에 따라 차이가 있었으며, 두 가지 성분들의 함량에 따라 항산화효과 및 tyrosinase 활성 저해효과가 다르게 나타났다. 이러한 연구 배경으로 calycosin과 formononetin을 유효성분으로 함유하는 황기추출물의 피부주름개선 효과를 알아보기 위해 콜라겐 발현효과 및 MMP-1 발현 억제효과를 각각 측정하였다. 그 결과 황기추출물의 콜라겐합성은 증가하였으나, MMP-1 발현 저해효과는 크지 않았다. 또한 0.1% 황기추출물 함유 크림은 대조군에 비하여 피부주름 개선효과를 나타냈다. 이러한 결과를 볼 때 황기추출물은 주름개선용 기능성화장품 신소재로의 응용이 기대된다.

당뇨환자의 신경병증 통증과 혈당조절정도 및 자가관리간의 관계 (The Relationship between Neuropathic Pain and Glycemic Control, Self Management in Type II Diabetes Mellitus Patients)

  • 서영미;최원희
    • 한국산학기술학회논문지
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    • 제14권4호
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    • pp.1774-1780
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    • 2013
  • 본 연구는 당뇨 환자를 대상으로 신경병증 통증과 혈당조절정도 및 자가관리간의 관계를 파악하는 서술적 상관관계 연구이다. 자료는 일 대학교 병원의 내분비 내과에서 2011년 5월 10일부터 5월 31일까지 당뇨로 방문한 환자 중 연구기준에 적합한 108명을 대상으로 자가보고식 설문지와 의무기록을 이용하여 수집되었다. 자료분석은 SPSS WIN 18.0을 이용하여 서술통계와 Pearson's correlation coefficient를 실시하였다. 연구 결과 당뇨환자의 통증은 $9.3{\pm}0.4$점이었으며 당화혈색소 수치는 7.5% 이상인 군이 33.3%를 차지하였고 자가관리는 $5.40{\pm}0.76$점이었다. 당뇨환자의 신경병증 통증은 혈당조절 정도가 양호할수록(r=.18, p=.035), 자가관리 수행정도가 높을수록(r=-.19, p=.023) 적었다. 결론적으로 당뇨환자의 신경병증 통증을 조절하기 위하여 보다 엄격한 혈당관리와 자가관리를 향상시키는 노력이 필요하다.

해산 녹조류 참홑파래, Monostroma nitidum의 원형질체 분리와 분화 (Protoplast Isolation and Differentiation of Marine Green Alga Monostroma nitidum)

  • 조용철;공용근;윤장택;선상미;정규화
    • 한국수산과학회지
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    • 제32권1호
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    • pp.117-120
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    • 1999
  • 해산 녹조류 참흩파래, Monostroma nitidum의 엽체를 효소처리하여 다량의 원형질체를 분리하였다. 최적 효소액의 조합은 $4\%$ R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power로서 생체조직 300mg 당 $4.41\times10^6$개의 원형질체가 분리되었다. 원형질체의 수율은 효소처리 270분에 최대였다. 분리 직후의 원형질체는 구형으로 직경 $13\~33\mu$m의 크기였다. 분리된 원형질체는 0.4M mannitol을 함유한 f/2배지에서 배양한 후 매주 mannitol이 함유되지 않은 f/2 배지로 절반씩 교환함으로서 분화율을 높일 수 있었다. f/2배지를 사용한 적정 배양조건에서 원형질체는 배양 3일 후 새로운 세포벽을 형성하였으며 10일 후 발아하기 시작하여 엽체로 발달하였다. 항생물질의 배지내 첨가는 배양체의 분화를 저해하였다.

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비닐포스폰산 비스-${\beta}$-클로로에틸과 초산비닐의 혼성중합 (Copolymerization of Bis(${\beta}$-chloroethyl)vinylphosphonate and vinyl Acetate)

  • 진정일;변회섭;박유미
    • 대한화학회지
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    • 제21권3호
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    • pp.219-224
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    • 1977
  • 비닐포스폰산 비스-${\beta}$-클로로에틸(BCVP)과 초산비닐(VAc)의 혼성중합을 $50^{\circ{C$에서 행하여 단위체 반응성비를 구하였다. 이때 과산화벤조일을 개시제로 사용하였으며, 얻은 반응성비는 아래와 같다. $r_1 (VAc) = 0.33,\;r_2 (BCVP) = 0.47$ 이 값과 Alfrey-Price식을 이용하여 비닐포스폰산 비스-${\beta}$-클로로에틸의 Q와 e값을 계산하니 Q = 0.06 및 e = 1.1을 얻었다. 이 두 단위체로부터 얻어지는 혼성중합체중의 단위체 서열분포와 평균서열 길이를 통계적으로 분석한 결과 혼성중합체가 비교적 높은 교대경향을 갖고 있음을 알았다. 또한 혼성중합체중에 비닐포스폰산 비스-${\beta}$-클로로에틸의 함량이 많을 수록 열안정성이 나뻐짐을 열시차분석에서 관찰하였다.

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다공성 폴리에틸렌을 이용한 하악골 함몰의 교정: 증례보고 (The Correction of the Mandibular Defect Using Porous Polyethylene: Case Report)

  • 임재형;김수관;문성용;오지수;박진주;정미애
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제33권4호
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    • pp.363-367
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    • 2011
  • Genioplasy with osteotomy is a flexible and useful procedure. However, osteotomy can cause different types of morbidity. Chin augmentation with porous polyethylene ($Medpor^{(R)}$) has become popular in the recent years. Porous polyethylene ($Medpor^{(R)}$) is an excellent biomaterial for reconstructing facial deformities. $Medpor^{(R)}$ has a porous architecture, which prevents capsule formation and decreases the degree of foreign-body reaction. In addition, it can be easily cut with scissors and molded, and it also maintains its shape. We report here on a satisfactory case of chin augmentation with using porous polyethylene ($Medpor^{(R)}$).

한국인의 치은연하 치태에서 Fusobacterium nucleatum의 분리 (Isolation of Fusobacterium nucleatum from subgingival plaque in Korean)

  • 장현선;김세훈;김화숙;국중기;김미광;유소영;김병옥
    • Journal of Periodontal and Implant Science
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    • 제33권2호
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    • pp.149-158
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    • 2003
  • The purpose of this study was to isolate and characterize the Fusohacrerium nucleatum (F. nucleatum) from subgingival plaque in Korean periodontitis patients. The subgingival plaque samples of periodontitis patient were collected with sterilized paper point. The paper point was put into reduced transfer medium and then immediately transferred to laboratory. The subgingival samples were diluted by 10,000 folds and plated on F. nucleatum-selective media agar plate. The plates were incubated at 37$^{\circ}C$ in an anaerobic chamber for 3 days. The violet-colored colonies were selected and subjected to further verification whether those are F. nucleatum or not. For further confirmation, 16S rRNA genes (rDNA) were cloned from each of bacterial clones and determined sequence of 16S rDNA. In this study, we found 17 distinct clinical isolates of F. nucleatum from subgingival plaque. The clinical isolates will be a useful in various studies in periodontology.

초등학생의 코로나19에 대한 인식과 손 씻기 지식, 태도 및 실천 (Perception of COVID-19, and Knowledge, Attitude and Practice of Hand washing in Elementary School Students)

  • 이영희;유미애
    • 한국보건간호학회지
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    • 제36권1호
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    • pp.20-32
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    • 2022
  • Purpose: This study aimed to investigate factors affecting handwashing practice among elementary school students by assessing the perception of coronavirus disease-19 (COVID-19) and characteristics related to knowledge, attitude, and practice of handwashing. Methods: The cross-sectional study design and an online self-reported survey were used in this study. The subjects were 211 elementary students at a school located in G province, Korea. Data were collected from January 4 to 8, 2021. The data were analyzed using SPSS version 25.0. Results: Mean scores for knowledge, attitude, and practice of handwashing were 17.09 (out of 20), 21.15 (out of 24), and 27.51 (out of 33), respectively. There were significant positive correlations between the perception of COVID-19 and knowledge of handwashing (r=.23, p=.001), between knowledge and attitude toward handwashing (r=.45, p<.001), between knowledge and practice of handwashing (r=.18, p=.010), and between attitude and practice of handwashing (r=.28, p<.001). The factors influencing handwashing practice were handwashing frequency at home (β=.39, p<.001), handwashing frequency at school (β=.18, p=.006), and attitude toward handwashing (β=.15, p=.026), which explained 34.2% of the variance. Conclusion: These results suggest a need to raise awareness of the importance of handwashing and develop an education program for infectious disease prevention.