• 디지털융복합연구
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• 제19권9호
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• pp.453-461
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• 2021

본 연구는 중소병원 간호사의 환자안전에 대한 태도, 도덕적 민감성, 표준주의지침 수행과의 관련성을 파악하고, 표준주의지침 수행에 영향을 미치는 요인을 규명하기 위해 시도되었다. 자료수집은 2021년 1월 11일부터 1월 22일까지 중소병원에 근무하는 150명의 간호사를 대상으로 설문조사를 시행하였다. 자료분석은 Descriptive statistics, Independent t-test, one-way ANOVA, Pearson's correlation coefficient, Multiple regression analysis를 이용하였다. 연구결과 표준주의지침 수행에 환자안전에 대한 태도 (r=.539, p<.001), 도덕적 민감성(r=.249, p=.002)에 유의한 상관관계가 있는 것으로 나타났다. 다중회귀분석결과 중소병원 간호사의 표준주의지침 수행에 영향을 미치는 요인은 환자안전에 대한 태도와 근무부서로 설명력은 33.8%이었다(F=18.47, p<.001). 연구결과를 통해 중소병원 간호사의 표준주의지침 수행을 높이기 위해서는 환자안전에 대한 올바른 태도를 정립할 수 있는 교육프로그램 개발이 필요하다.

• Molecules and Cells
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• 제40권8호
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• pp.587-597
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• 2017

MicroRNAs (miRNAs) are essential small RNA molecules that regulate the expression of target mRNAs in plants and animals. Here, we aimed to identify miRNAs and their putative targets in Hibiscus syriacus, the national flower of South Korea. We employed high-throughput sequencing of small RNAs obtained from four different tissues (i.e., leaf, root, flower, and ovary) and identified 33 conserved and 30 novel miRNA families, many of which showed differential tissuespecific expressions. In addition, we computationally predicted novel targets of miRNAs and validated some of them using 5' rapid amplification of cDNA ends analysis. One of the validated novel targets of miR477 was a terpene synthase, the primary gene involved in the formation of disease-resistant terpene metabolites such as sterols and phytoalexins. In addition, a predicted target of conserved miRNAs, miR396, is SHORT VEGETATIVE PHASE, which is involved in flower initiation and is duplicated in H. syriacus. Collectively, this study provides the first reliable draft of the H. syriacus miRNA transcriptome that should constitute a basis for understanding the biological roles of miRNAs in H. syriacus.

• 대한치과의료관리학회지
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• 제6권1호
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• pp.11-18
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• 2018

The aim of this study was to evaluate the competency of the Easyperio test, a genetic test method based on real time PCR for the detection of bacteria that cause dental caries and periodontal disease. To verify the validity of this text, various dental health evaluations were administered to 33 boys between the ages of 12 to 14, as this age group commonly experiences dental caries. These evaluations included a dental caries experience survey, a first molar health evaluation, the Dentocult Streptococcus mutans (SM) strip mutans, the Dentocult Lactobacillus spp (LB) test, and the Easyperio test. The correlation coefficients between the level of the Dentocult SM strip mutans and the dental caries experience were DT (R=0.570, p=0.001), DMFT (R=0.376, p=0.031), and first molar health (R=-0.395, p=0.023). The correlation coefficients between the amount of SM in the Easyperio test and dental caries experience were DT (R=0.528, p=0.002), DMFT (R=0.369, p=0.035), and first molar health (R=-0.426, p=0.013). The correlation coefficients between the level of the dentocult SM strip mutans and the SM amounts of the Easyperio test were S.mi (R=0.564 p=0.001) and S.mu (R=0.621, p=0.002). The correlation coefficients between the level of the Dentocult LB test and the SM amount of Easyperio test was S.mi (R=0.495, p=0.003). In conclusion, Easyperio test may be an easy and effective method for the differentiation and diagnosis of dental caries through quantitative and qualitative analysis of oral bacteria.

• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.219-229
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• 2020

Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORT^TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

• 한국컴퓨터정보학회논문지
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• 제24권5호
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• pp.113-120
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• 2019

This study was conducted to identify the level of cultural empathy, self-esteem, and cultural competence and factors affecting cultural competence of nursing students. Participants were 157 nursing students who were 2nd, 3rd, and 4th grade in one university located at the Chungbuk provinces. Data collected using questionnaires which consisted of general characteristics, cultural empathy, self-esteem, and cultural competence. The mean score for cultural empathy was $3.63{\pm}.34$(scores ranged from 1 to 5), The mean score for self-esteem was $2.91{\pm}.42$(scores ranged from 1 to 4). The mean score for cultural competence was $3.31{\pm}.43$(scores ranged from 1 to 5). There were significant differences between cultural competence and age(t=-2.242, p=.027), grade(F=3.473, p=.033), and fluency of foreign language(F=5.053, p=.007). There were significant correlation between cultural competence and cultural empathy(r=.49, p<.001), and self-esteem(r=.33, p<.001)), and self-esteem and cultural empathy(r=.33, p<.001). Cultural empathy and self-esteem explained 29.4% of cultural competence and major predictor variable for cultural competence was cultural empathy. In this paper, we propose various multicultural education programs in curriculum and extra curriculum that can enhance cultural empathy and self-esteem to improve cultural competence of nursing students. Especially, continuous education of cultural knowledge among cultural competence will be done.

• 과학교육연구지
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• 제36권1호
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• pp.14-21
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• 2012

본 연구에서는 뇌 활용 성향과 수학 및 과학 학업성취도 간의 관계를 분석하고자 중학생 131명(남: 74, 여: 57)을 대상으로 뇌 활용 성향검사를 실시하고 수학 및 과학 과목의 성적을 조사하였다. 수학 성적과 과학 성적에 따라 집단을 구분하였을 때 수학 성적 상위집단과 과학 성적 상위집단 모두 좌하뇌를 주로 활용하는 학생의 수가 가장 많은 것으로 나타났다(수학: 33.8%, 과학: 35.0%). 반면에, 수학 성적 하위집단과 과학 성적 하위집단은 우하뇌를 주로 활용하는 학생의 수가 가장 많은 것으로 나타났다(수학: 38.7%, 과학: 36.1%). 수학 및 과학 성적 상위집단과 하위집단간에 뇌 활용 성향 점수의 차이를 분석한 결과 과학성적 상위집단과 하위집단 간에는 좌하뇌 점수(p<.05)에서 차이를 나타내었다. 상관도 분석결과 수학 성적과 과학 성적 모두 좌하뇌 점수(수학: r=.28, 과학: r=.26)에서 가장 높은 상관도를 보였으며 좌상뇌 점수(수학: r=.18, 과학: r=.24)와 두 번째로 높은 상관도를 보였다. 이러한 결과는 수학 및 과학 과목에서 우수한 성적을 얻는 것이 좌하뇌 및 좌상뇌의 활용 성향과 연관성이 있다는 것을 보여주는 것으로 수학 및 과학 과목의 평가방법의 개발 시 이러한 특성을 고려할 필요성이 있을 것이다.

• 지역사회간호학회지
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• 제14권4호
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• pp.686-698
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• 2003

Purpose: This is a descriptive correlation study purposed to provide basic data for comprehensive nursing care by analyzing the relationship between the burden and the quality of life of family caregivers of cancer patients treated with chemotherapy. Methods: As for the subjects of this study, 66 family caregivers who take care of cancer patients hospitalized and treated with chemotherapy participated in the research. The tool of this study was a structured questionnaire including questions concerning burden(19 items) and the quality of life(33 items). Collected data were analyzed using SPSS PC+ program through descriptive statistics, t-test, ANOVA, Pearson's correlation coefficient, and stepwise multiple linear regression. Results: 1) The mean score of burden was 2.6383. The mean score of quality of life was 3.3034. These score show that family caregivers perceive a moderate level of burden and the quality of life. 2) Family caregivers' burden was significantly related to symptoms in the cancer patient (r= 0.3501, P=0.0042) and family caregivers(r= 0.5340, P=0.0001). Family caregivers' quality of life was significantly related to symptoms in the cancer patient(r= -0.3528, P=0.0039) and family caregivers(r= -0.5472, P=0.0001). According to the result of examining' the relationship between burden and the quality of life, there was a statistically negative correlation(r= -0.6326, P=0.0001). 3) 'Family income after the onset of the patient' was the most important predictor of the burden of family caregivers($R^2$=0.158). 'Usual relationship with the patient' was the most important predictor of the quality of life of family caregivers($R^2$=0.138). Conclusions: The results presented above indicate that we must consider burden of family caregivers and symptoms experienced by them in order to improve the quality of life of family caregivers of cancer patients treated with chemotherapy.

• 한국산학기술학회논문지
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• 제17권12호
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• pp.81-90
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• 2016

본 연구의 목적은 대학생을 대상으로 플립러닝을 적용한 교육방법의 효과를 알아보고자 하는 것이다. 플립러닝을 적용하기 전과 후의 자기주도학습 준비도, 핵심역량을 파악하고, 플립러닝을 적용한 후 교수-학생 상호작용, 학습만족도, 학습동기를 파악하여, 플립러닝 적용 후 각 변수의 상관관계를 살펴보고, 학습동기에 미치는 영향을 알아보고자 하였다. 단일군 전후 비교 연구로 대상자는 경기도 S시 일개 간호대학에서 플립러닝을 활용하여 건강사정 및 실습을 수강하는 2학년 학생을 대상으로 하였다. 최종 분석은 68명을 대상으로 하였고, 사전 사후의 차이는 paired t-test로, 각 변수간 상관관계는 Pearson's correlation coefficient로 분석하였으며, 종속변수인 학습동기에 미치는 영향은 단계적 다중회귀방법을 사용하였다. 그 결과 자기주도학습 준비도는 사전 사후 통계적으로 유의하게 증가하였고, 핵심역량은 그 차이가 유의하지 않았다. 플립러닝교육 후 학습동기는 자기주도학습 준비도(r=0.33, p=.006), 대학생 핵심역량(r=0.51, p<.001), 교수-학생 상호작용(r=0.72, p<.001), 학습만족도(r=0.79, p<.001)와 유의한 정적 상관관계를 보였고, 모든 다른 변수들 간에도 정적으로 유의한 상관관계를 보였다. 학습동기에 영향을 미치는 요인은 학습만족도와 교수-학생 상호작용이었으며, 이 2개 변수로 구축된 회귀모형의 학습동기에 대한 설명력은 71.3% (F=80.66, p<.001)였다. 따라서 플립러닝 교육을 적용하였을 때 학습동기를 증진시키기 위해서는 학습만족도를 증진시키고, 교수-학생 상호작용을 활발히 하는 전략의 마련이 필요하겠다.

• 여성건강간호학회지
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• 제19권4호
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• pp.295-305
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• 2013

Purpose: The objective of this study was to identify the relationships among symptom bother, physical and mental stress and health-related quality of life (HRQoL) in women with overactive bladder (OAB) syndrome. Methods: The participants were 106 women who were diagnosed with OAB (urgency, urge urinary incontinence, frequency, and/or nocturia) at P university hospital. Data were collected from Dec 23, 2011 to Aug 31, 2012. Results: The mean score for symptom bother was 43.1 points, for physical stress, 12.8 which was slightly higher than mental stress (11.8), and for HRQoL, 63.9. For symptom type, there were statistically significant differences in the symptom bother (F=8.67, p<.001) and HRQL (F=3.32, p=.023). The Symptom bother of OAB was positively correlated with physical stress (r=.23, p=.014) and mental stress (r=.33, p<.001) and negatively correlated with the subscales of HRQoL; coping (r=-.66, p<.001), concern (r=-.71, p<.001), sleep (r=-.59, p<.001), and social interaction (r=-.58, p<.001). Conclusion: From the results, bother symptom was associated with physical, mental stress and HRQoL. These results suggest that nursing intervention programs for OAB should be developed not only to relieve the symptoms but also to reduce stress and improve the quality of life.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.138.2-139
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• 2003

The inhibitory effects of (1R, 9S)- and (1S, 9R)-enantiomers of $\beta$-hydrastine (BHS) on dopamine biosynthesis in PC12 cells were investigated. (1R, 9S)-BHS decreased the intracellular dopamine content with the $IC_{50}$ value of 14.3 $\mu\textrm{M}$ at 24 h, but (1S, 9R)-BHS did not. In these conditions, (1R, 9S)-BHS inhibited TH activity mainly in a concentration-dependent manner(33％ inhibition at 20 $\mu\textrm{M}$) and decreased TH mRNA level. (omitted)