• Molecules and Cells
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• v.38 no.10
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• pp.895-903
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• 2015

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unclear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysis in PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through which these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.

• Journal of Korean Medicine Rehabilitation
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• v.19 no.4
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• pp.115-126
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• 2009

Objectives : The aim of this study was to evaluate whether the foot pressure distribution correlates with the lumbo-sacral curvature, and the Oswestry Disability Index in chronic low back pain patients. Methods : We measured the fore foot pressure and the rare foot pressure using the foot analyzer in 28 women subjects with chronic low back pain. The lumbo-sacral curvature and the Oswestry Disability Index(ODI) were also measured. Results : 1. Subjects with higher ODI(%) had significantly lower Fore foot pressure/Rare foot pressure ratio(F/R ratio) (p<0.01). 2. Lumbar lordotic angle and Ferguson angle were inversely related to ODI(%) (p<0.05, p<0.01). 3. Lumbar lordotic angle and Ferguson angle were positively related to F/R ratio (all p<0.05). Conclusions : Using the Foot Analyzer(FA-48S, Tech storm Inc.) we have shown that F/R ratio has significant correlation with the lumbo-sacral curvature and the Oswestry Disability Index. These result suggest that the Foot analyzer may be used in assessing back pain in chronic low back pain patients.

• Fire Science and Engineering
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• v.24 no.6
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• pp.126-132
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• 2010

Due to the change of future environment like high-rise building and the use of basement space etc, new types of disaster occur in recent years. The scale of disasters is changing to bigger than past and the environment of fire industry is varied with Korea-America FTA etc. As the reasons above expressed, the necessity of technical innovation of fire industry comes to the fore. Domestic fire products don't have competitive technique than one of developed countries and also don't have competitive price than one of Southeast Asia and China. It is very important that the independent fire industry must be established using R&D vitalization to overcome these problems. In this study, we have analysed the status of domestic R&D and the domestic fire industry R&D. And we suggested the problems of above analysis and improvement. Consequently, we want to suggest the development strategy through R&D vitalization.

• Journal of Industrial Convergence
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• v.22 no.2
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• pp.119-126
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• 2024

The purpose of this study was to examine the effects of social support and Grit on academic self-efficacy among nursing students. Data were collected from October to November 2022 from nursing students in five nursing departments located in City D. A total of 167 subjects participated in the study. The collected data were analyzed using SPSS/WIN statistics program 26.0. As a result, social support(r=0.379, p<.001) and Grit(r=0.532, p<.001) were correlated with academic self-efficacy, and grit was identified as a factor affecting academic self-efficacy(Adju-R²=.277, p<.001). Based on the findings of this study, it is recommended that future programs be developed to increase academic self-efficacy in nursing students.

• Journal of Sensor Science and Technology
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• v.17 no.2
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• pp.120-126
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• 2008

Glucose concentrations in the interstitial fluid were measured based on optical spectroscopy. Prediction of glucose concentrations was made using partial least squares regression and accuracy improvement was achieved by data preprocessing as well as by selecting an optimal wavelength region. For this purpose, artificial interstitial fluid samples were prepared where their glucose levels varied between 0 and 10 g/dl. Infrared spectral regions where glucose absorption lies were investigated. A region of 1000 - 1500 $cm^{-1}$ produced the best accuracy among the regions of 1000 - 1500 $cm^{-1}$, 4000 - 4545 $cm^{-1}$1 and 5500 - 6500 $cm^{-1}$. Further accuracy improvement in 1000 - 1500 $cm^{-1}$ was achieved by selecting specific wavelength bands based on a loading vector analysis method. For the samples whose glucose concentrations ranged between 0 and 0.5 g/dl, SEP= 0.0266 g/dl and R =0.9863 were achieved with 1000 - 1500 $cm^{-1}$. However, the loading vector optimized band of 1002 - 1095 $cm^{-1}$ reduced the prediction error up to 47 % (SEP =0.0125 g/dl and R=0.9970).

• Parasites, Hosts and Diseases
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• v.37 no.3
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• pp.163-169
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• 1999

House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophogoides pteronyssinus, were produce. Four monoclonal antibodies produced wee species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p2 in house dust. The sensitivity limit was 4ng/ml with rDer p2 and $8{\;}\mu\textrm{g}/ml$ with the d. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

• Journal of the Korean Society of Radiology
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• v.84 no.1
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• pp.240-252
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• 2023

Purpose To assess the effect of deep learning image reconstruction (DLIR) for head CT in pediatric patients. Materials and Methods We collected 126 pediatric head CT images, which were reconstructed using filtered back projection, iterative reconstruction using adaptive statistical iterative reconstruction (ASiR)-V, and all three levels of DLIR (TrueFidelity; GE Healthcare). Each image set group was divided into four subgroups according to the patients' ages. Clinical and dose-related data were reviewed. Quantitative parameters, including the signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR), and qualitative parameters, including noise, gray matter-white matter (GM-WM) differentiation, sharpness, artifact, acceptability, and unfamiliar texture change were evaluated and compared. Results The SNR and CNR of each level in each age group increased among strength levels of DLIR. High-level DLIR showed a significantly improved SNR and CNR (p < 0.05). Sequential reduction of noise, improvement of GM-WM differentiation, and improvement of sharpness was noted among strength levels of DLIR. Those of high-level DLIR showed a similar value as that with ASiR-V. Artifact and acceptability did not show a significant difference among the adapted levels of DLIR. Conclusion Adaptation of high-level DLIR for the pediatric head CT can significantly reduce image noise. Modification is needed while processing artifacts.

• Journal of Korean Clinical Nursing Research
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• v.24 no.2
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• pp.255-262
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• 2018

Purpose: This study aimed to identify the view of life and death among ICU nurses and to analyze the problems related to end-of-life care in the current ICUs. Methods: A descriptive study design was used. The participants were 975 nurses working in the intensive care units of 16 general hospitals. Using a descriptive survey design, data were collected from August to December in 2016 and were analyzed using descriptive statistics, t-test, ANOVA, correlation analysis, and multiple regression analysis. Results: As a result of a correlation analysis of the data, Death perception had a significant positive correlation with EOL of nursing attitudes(r=.100, p=.002), and negative correlation with EOL stress care(r=-.221, p=<.001). The regression model explained for individual characteristics in the model, age(${\beta}=.126$, p<.001) and death perception(${\beta}=.182$, p<.001), Satisfaction of the EOL care(${\beta}=.173$, p<.001), Healing training needs on the EOL(${\beta}=-.144$, p<.001) were the most influential factors for EOL stress. Conclusion: Results reveal that ICU nurses have a moderate level of EOL stress, and that individual, age, death perception, Satisfaction of the EOL care, Healing traning needs on the EOL relevant in ICU nurses' EOL stress. Programs or interventions to reduce EOL stress and to should be developed taking into account these multidimensional factors.

• Journal of the Korean Society of School Health
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• v.31 no.2
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• pp.117-126
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• 2018

Purpose: The leading cause of death among teenagers in Korea is suicide. Also, it is known that mental health of Korean adolescents identified based on the levels of perceived stress or depression is very poor. The purpose of this study was to examine the current status of student mental health programs in Korea and the associated problems and to explore ways to make the programs more effective. Methods: The subjects of the study were a total of 115 school nurses and school counselors in elementary, middle and high schools. The study examined the current status and the associated problems of school mental health programs as well as the needs for teacher training programs related to student mental health. Results: Few mental health programs other than counseling for at-risk students were being carried out. The problems associated with student mental health management were, in terms of management, 'accountability in case of incidents such as student suicide' and 'parents uncooperative in their kid's mental health management (visit to hospital, etc.)' and, in terms of manpower, 'unclear R&R between school nurses and school counselors' and, more fundamentally, 'the lack of human resources.' The needs for teacher training programs related to student mental health were very high and the content required most was 'ways to manage stress of students and teachers themselves,' followed by 'interpersonal conflicts management & conservational skills,' and 'ways to manage student mental illness.' Conclusion: In conclusion, to increase the effectiveness of student mental health programs, we should move on from the current system which focuses only on early discovery and intervention to a more comprehensive system that includes preventive approaches, while having clearer R&R and more workforce dedicated to student mental health programs and attracting more parental cooperation and support.

• Journal of Yeungnam Medical Science
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• v.38 no.1
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• pp.34-38
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• 2021

Background: We aimed to determine whether routine second trimester complete blood cell (CBC) count parameters, including neutrophil-lymphocyte ratio (NLR), lymphocyte-monocyte ratio (LMR), and platelet-lymphocyte ratio (PLR), could predict obstetric outcomes. Methods: We included singleton pregnancies for which the 50-g oral glucose tolerance test and CBC were routinely performed between 24 and 28 weeks of gestation in our outpatient clinic from January 2015 to December 2017. The subjects were divided into three groups according to their pregnancy outcomes as follows: group 1, spontaneous preterm births, including preterm labor and preterm premature rupture of membranes; group 2, indicated preterm birth due to maternal, fetal, or placental causes (hypertensive disorder, fetal growth restriction, or placental abruption); and group 3, term deliveries, regardless of the indication of delivery. We compared the CBC parameters using a bivariate correlation test. Results: The study included 356 pregnancies. Twenty-eight subjects were in group 1, 20 in group 2, and 308 in group 3. There were no significant differences between the three groups in neutrophil, monocyte, lymphocyte, and platelet counts. Although there was no significant difference in NLR, LMR, and PLR between the three groups, LMR showed a negative correlation with gestational age at delivery (r =-0.126, p =0.016). Conclusion: We found that a higher LMR in the second trimester was associated with decreased gestational age at delivery. CBC parameters in the second trimester of pregnancy could be used to predict adverse obstetric outcomes.