Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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PGC-Enriched miRNAs Control Germ Cell Development

  • Bhin, Jinhyuk (Department of Chemical Engineering, Pohang University of Science and Technology) ;
  • Jeong, Hoe-Su (Graduate School of Biomedical Science and Engineering, Department of Biomedical Science, Hanyang University) ;
  • Kim, Jong Soo (Graduate School of Biomedical Science and Engineering, Department of Biomedical Science, Hanyang University) ;
  • Shin, Jeong Oh (Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University, College of Dentistry) ;
  • Hong, Ki Sung (Graduate School of Biomedical Science and Engineering, Department of Biomedical Science, Hanyang University) ;
  • Jung, Han-Sung (Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University, College of Dentistry) ;
  • Kim, Changhoon (Graduate School of Biomedical Science and Engineering, Department of Biomedical Science, Hanyang University) ;
  • Hwang, Daehee (Department of Chemical Engineering, Pohang University of Science and Technology) ;
  • Kim, Kye-Seong (Graduate School of Biomedical Science and Engineering, Department of Biomedical Science, Hanyang University)
  • Received : 2015.05.26
  • Accepted : 2015.07.08
  • Published : 2015.10.31
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Abstract

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unclear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysis in PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through which these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.

Keywords

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