• Tuberculosis and Respiratory Diseases
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• 제80권3호
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• pp.270-276
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• 2017

Background: A disputed rpoB mutation is a specific type of rpoB mutation that can cause low-level resistances to rifampin (RIF). Here, we aimed to assess the frequency and types of disputed rpoB mutations in Mycobacterium tuberculosis isolates from South Korea. Methods: Between August 2009 and December 2015, 130 patients exhibited RIF resistance on the MTBDRplus assay at Asan Medical Center. Among these cases, we identified the strains with disputed rpoB mutation by rpoB sequencing analysis, as well as among the M. tuberculosis strains from the International Tuberculosis Research Center (ITRC). Results: Among our cases, disputed rpoB mutations led to RIF resistance in at least 6.9% (9/130) of the strains that also exhibited RIF resistance on the MTBDRplus assay. Moreover, at the ITRC, sequencing of the rpoB gene of 170 strains with the rpoB mutation indicated that 23 strains (13.5%) had the disputed mutations. By combining the findings from the 32 strains from our center and the ITRC, we identified the type of disputed rpoB mutation as follows: CTG511CCG (L511P, n=8), GAC516TAC (D516Y, n=8), CTG533CCG (L533P, n=8), CAC526CTC (H526L, n=4), CAC526AAC (H526N, n=3), and ATG515GTG (M515V, n=1). Conclusion: Disputed rpoB mutations do not seem to be rare among the strains exhibiting RIF resistance in South Korea.

• Genomics & Informatics
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• 제21권4호
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• pp.44.1-44.10
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• 2023

Tumor hypoxia, oxygen deprivation state, occurs in most cancers and promotes angiogenesis, enhancing the potential for metastasis. The vascular endothelial growth factor (VEGF) family genes play crucial roles in tumorigenesis by promoting angiogenesis. To investigate the malignant processes triggered by hypoxia-induced angiogenesis across pan-cancers, we comprehensively analyzed the relationships between the expression of VEGF family genes and hypoxic microenvironment based on integrated bioinformatics methods. Our results suggest that the expression of VEGF family genes differs significantly among various cancers, highlighting their heterogeneity effect on human cancers. Across the 33 cancers, VEGFB and VEGFD showed the highest and lowest expression levels, respectively. The survival analysis showed that VEGFA and placental growth factor (PGF) were correlated with poor prognosis in many cancers, including kidney renal cell and liver hepatocellular carcinoma. VEGFC expression was positively correlated with glioma and stomach cancer. VEGFA and PGF showed distinct positive correlations with hypoxia scores in most cancers, indicating a potential correlation with tumor aggressiveness. The expression of miRNAs targeting VEGF family genes, including hsa-miR-130b-5p and hsa-miR-940, was positively correlated with hypoxia. In immune subtypes analysis, VEGFC was highly expressed in C3 (inflammatory) and C6 (transforming growth factor β dominant) across various cancers, indicating its potential role as a tumor promotor. VEGFC expression exhibited positive correlations with immune infiltration scores, suggesting low tumor purity. High expression of VEGFA and VEGFC showed favorable responses to various drugs, including BLU-667, which abrogates RET signaling, an oncogenic driver in liver and thyroid cancers. Our findings suggest potential roles of VEGF family genes in malignant processes related with hypoxia-induced angiogenesis.

• 한국융합학회논문지
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• 제9권2호
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• pp.43-50
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• 2018

본 연구의 목적은 만성요통을 가진 여성 노인의 자기 효능감과 건강행위를 파악하기 위함이다. 본 연구는 2012년 1월부터 10월까지 G도에 있는 종합병원 정형외과 및 신경외과 병동에 요추 및 천추의 척추질환을 진단받고 6개월 이상 요통을 경험하고 요통치료를 받기 위해 입원한 65세 이상의 여성 노인 환자 130명을 대상으로 자기 효능감과 건강행위간의 관계를 분석하기 위해 상관관계분석 방법을 사용하였다. 본 연구에서 자기효능감과 건강 행위간에는 통계적으로 유의한 양의 상관관계가 나타난 것을 확인할 수 있었고, 만성 요통을 가진 여성 노인 대상자의 자기 효능감 및 건강행위는 서로 관련이 있다는 것을 확인하였다(r=.80, p<.001). 본 연구 결과를 통하여 만성요통을 가진 여성 노인의 건강행위 증진을 위해 자기효능감을 강화시키는 것이 필요함을 알 수 있었다. 따라서 만성 요통을 가진 여성 노인의 간호시 자기 효능감을 증진시키고, 건강 행위에 긍정적인 영향을 줄 수 있는 융복합적 전략이 고려되어야 한다.

• 한국직업건강간호학회지
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• 제22권2호
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• pp.130-139
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• 2013

Purpose: This study was to identify the relationship among clinical nurse's job overload, self-efficacy, emotional exhaust and turnover intention. Methods: The study was conducted through a survey on 537 clinical nurses in five general hospitals. The data were collected by self-reporting questionnaires and analysed by the SPSS/WIN 17.0 program using descriptive statistics, t-test, ANOVA, Pearson correlation coefficient and stepwise multiple regression. Results: The mean score of turnover intention was 3.04. Turnover intention according to age, total career, job objective, and work off showed significant differences. The significant positive correlations between emotional exhaust and turnover intention (r=.531, p<.001), job overload and turnover intention (r=.514, p<.001) were found. The significant factors influencing turnover intention were emotional exhaust (${\beta}$=.488, p<.001), career (${\beta}$=.175, p<.001), job overload (${\beta}$=.119, p= .003), and number of night (${\beta}$=.117, p=.002), which accounted for 40.3% of the variance. Conclusion: Thought this result, nursing managers should analyze the reasons for high turnover intention, focusing on the main factors affecting turnover intention and consider the solutions to decrease the turnover intention.

• Journal of Korean Biological Nursing Science
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• 제19권3호
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• pp.123-130
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• 2017

Purpose: This study aimed to identify factors influencing intra-operative core body temperature (CBT), and to develop a predictive model for intra-operative CBT in laparoscopic abdominal surgery. Methods: The prospective observational study involved 161 subjects, whose age, weight, and height were collected. The basal pre-operative CBT, pre-operative blood pressure, and heartbeat were measured. CBT was measured 1 hour and 2 hours after pneumoperitoneum. Results: Explanatory factors of intra-operative hypothermia (< $36^{\circ}C$) were weight (${\beta}=.361$, p< .001) and pre-operative CBT (${\beta}=.280$, p= .001) 1 hour after pneumoperitoneum (Adjusted $R^2=.198$, F= 7.56, p< .001). Weight was (${\beta}=.423$, p< .001) and pre-operative CBT was (${\beta}=.206$, p= .011) 2 hours after pneumoperitoneum (Adjusted $R^2=.177$, F= 5.93, p< .001). The researchers developed a predictive model for intra-operative CBT ($^{\circ}C$) by observing intra-operative CBT, body weight, and pre-operative CBT. The predictive model revealed that intra-operative CBT was positively correlated with body weight and pre-operative CBT. Conclusion: Influence of weight on intra-operative hypothermia increased over time from 1 hour to 2 hours after pneumoperitoneum, whereas influence of pre-operative CBT on intraoperative hypothermia decreased over time from 1 hour to 2 hours after pneumoperitoneum. The research recommends pre-warming for laparoscopic surgical patients to guard against intra-operative hypothermia.

• 의료커뮤니케이션
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• 제13권2호
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• pp.149-158
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• 2018

Background: This study was to survey the effect of the degree of spiritual nursing care performance on the spiritual nursing care ability of the nursing students Methods: The researcher sampled 130 nursing students for a questionnaire survey conducted from September 17 until September 27, 2018. The data of analysis used SPSS 23.0 program. Results: The spiritual nursing care ability was $4.4{\pm}0.8$(total score 6) and the degree of spiritual nursing care performance was $2.9{\pm}1.8$(total score 4). The spiritual nursing care ability differed significantly depending on religion(F=7.570, p<.001), the level of spiritual nursing knowledge(F=19.873, p<.001), education type(F=14.626, p<.001), necessity of hospice(t=2.280, p=.024). The degree of spiritual nursing care performance differed significantly depending on spiritual nursing education time(F=2.932, p=.036). The correlation of two variable was statistically significant difference(r=.206, p=.019). The influencing factors on the spiritual nursing care ability was religion, the level of spiritual nursing knowledge, education type, dying experience($R^2=0.378$, Adj $R^2=0.353$), the degree of spiritual nursing care performance was spiritual nursing education time($R^2=0.065$, Adj $R^2=0.043$). Conclusion: These results show that nursing students are not able to perform spiritual nursing care properly to subjects who need spiritual nursing care. Therefore, it is necessary to develope programs to meet the spiritual nursing care needs of nursing students and to provide practical education in accordance with the program by cooperating with the nursing education staff, clergy and clinical nurse etc. And spiritual nursing care should be taught as a required subjects in the curriculum.

• 한국산학기술학회논문지
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• 제21권5호
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• pp.121-130
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• 2020

간호 대학생의 실습 전, 후 임상실습 스트레스, 수면의 질, 주간 졸림증 차이와 관계를 확인하는데 목적이 있다. 연구대상은 간호학과 3학년 학생 130명이다. 연구결과 연구 대상자의 임상실습 스트레스는 총점 5점 만점 중 평균점수 3.15점으로 나타났다. 임상실습 스트레스의 외부조정 요인 중 과제물에 대한 점수가 3.64로 가장 높았고, 성별(t=-3.88, p<.001), 주관적 건강상태(F=5.78, p=.004), 거주형태(F=3.20, p=.026)에 유의한 차이가 있는 것으로 나타났다. 간호대학생은 수면의 질이 낮고(실습 전 80.0%, 실습 후 70.0%), 주간 졸림증이 있는 대상이(실습 전 26.9%, 실습 후 42.3%) 일반 대학생에 비해 많음을 확인 할 수 있었다. 임상실습 전, 후 수면의 질은(χ²=4.06, p=.004), 주간 졸림증은(χ²=6.08, p=.009)로 유의한 차이가 있었다. 또한 주간 졸림증과 수면의 질은 음의 상관관계(r=-.259, p<0.01)가 있는 것으로 나타났다. 따라서 간호 대학생들의 신체적 건강 및 정신적 건강을 위하여 임상실습 스트레스를 줄이고 수면의 질을 높일 수 있는 전략 개발이 필요하며, 변화하는 간호 교육현장에서 임상실습 적응에 도움이 되도록 하여 추후 간호의 질을 향상시킬 수 있도록 해야겠다.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.211-216
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• 2001

The growth of Selenastrum capricornutum in culture was investigated as a function of ionic copper concentrations to verify the toxicity and physiological variation. In a $Cu^{++}$ excess culture (E: $Cu^{++}$ of $130{\mu}g/l$), the growth rate (K)(0.32) was lower than that of the control culture (C: $Cu^{++}$ of $0.065{\mu}g/l$) (0.61) after 8 days and the exponential growth rate ($R_E$) was also found to be lower in culture E (1.1) than culture C (2.9). On the contrary, the K of S. capricornutum in trace $Cu^{++}$ culture ($T_1$ and $T_2$, 0.72) after 6 days tended to be more increased than culture C (0.68). From 8 to 14 days of culture, the amounts of chlorophyllls a and b were increased in culture C (chlorophyll a, $106->126{\mu}g/g$ dry wt; chlorophyll b, $158->208{\mu}g/g$ dry wt; chlorophyll b were decreased in culture E (chlorophyll a, $309->235{\mu}g/g$ dry wt; chlorophyll b, $405->352{\mu}g/g$ dry wt). The amounts of chlorophylls in ionic copper trace culture ($T_1$ and $T_2$) [(chl a/b) of $T_1$: $384/620\;{\mu}g/g$ dry wt; (chl a/b) of $T^2$:$320/467{\mu}g/g$ dry wt] were increased more than the culture C($260/387{\mu}g/g$ dry wt). Howeveer, when photosynthetic rates were normalized to the dry weight of algae, the control culture continued to showed higher values than the treated culture ($T_1$). An appropriate amount of ionic copper ($T_1:\;26\;{\mu}g/l$) stimulated the grwoth of S. capricornutum than the ionic copper content of $13\;{\mu}g/l$ ($T_2$), while the excess amount of ionic copper ($130\;{\mu}g/l$) resulted in the highest toxicity to the growth of S. capricornutum.

• Journal of Applied Biological Chemistry
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• 제57권1호
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• pp.47-51
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• 2014

등심초(Juncus effusus)를 70% ethanol로 추출하고 이 추출물을 ethyl acetate분획, n-butanol 및 $H_2O$ 분획으로 나누었다. Ethyl acetate 분획에 대하여 silica gel, Sephadex LH-20 column chromatography를 반복 실시하여 2종의 화합물을 분리하였다. 화합물 1 (dehydroeffusol)과 2 (effusol)에 대하여 free radical 소거활성을 측정한 결과, dehydroeffusol (DPPH; $130{\pm}3.21{\mu}M$, ABTS; $39{\pm}3.51{\mu}M$)과 effusol (DPPH; $79{\pm}1.53{\mu}M$, ABTS; $24{\pm}2.73{\mu}M$)은 강한 항산화 활성을 나타내었다. 또한 이들 화합물들의 인체유래 위암세포(AGS), 폐암세포(A549)에 대한 성장억제 효과를 측정한 결과 모두 항암약물(CPA)과 유사하거나 더 높은 성장 저해 효과를 보였다.

• 한국환경생태학회지
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• 제27권5호
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• pp.571-578
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• 2013

본 연구는 도시의 주요 상록 조경수종인 소나무와 잣나무를 대상으로, 직접수확법을 통해 탄소저장 및 흡수를 용이하게 추정하는 회귀모델을 제시하고 도시수목의 탄소저감 효과를 계량화하는데 필요한 기반정보를 구축하였다. 수종별로 유목에서 성목에 이르는 일정 간격의 흉고직경 크기를 고려하여 개방 생장하는 조경수목을 구입한 후, 근굴취를 포함하는 직접수확법에 의해 개체당 부위별 및 전체 생체량을 측정하고 탄소저장량을 산출하였다. 또한, 흉고 부위의 수간원판을 채취하여 직경생장을 분석하고 탄소흡수량을 산정하였다. 흉고직경을 독립변수로 생장에 따른 수종별 단목의 탄소저장 및 흡수를 계량화하는 활용 용이한 회귀모델을 유도하였다. 이들 회귀식의 $r^2$는 0.98 이상으로서 적합도가 상당히 높았다. 동일 직경의 탄소저장 및 흡수량은 유목의 경우 소나무가 잣나무보다 더 많았으나, 20cm 이상 성목의 경우 생장량 차이에 기인하여 그 반대인 경향을 보였다. 흉고직경 25cm인 소나무와 잣나무는 각각 115.6kg, 130.0kg의 탄소를 저장하고, 연간 9.4kg, 14.6kg의 탄소를 흡수하는 것으로 나타났다. 본 연구는 조경수목의 직접 벌목 및 근굴취의 난이성에 기인하여, 생체량 확장계수, 지하부/지상부 비율, 직경생장 등 산림수목의 계수를 대용하여 대상수종의 탄소저감을 계량화한 기존 연구의 한계성을 극복할 새로운 초석을 마련하였다.