• Title/Summary/Keyword: metacercaria

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The Localization of the Specific Antigenic Protein in the Tissue of Paragonimus westermani Metacercaria (폐흡충 피낭유충 조직에 있어서 특정항원성 단백질의 분포)

  • Kim, Soo-Jin;Roh, Tae-Hoon;Joo, Kyoung-Hwan;Rim, Han-Jong
    • Applied Microscopy
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    • v.27 no.4
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    • pp.403-416
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    • 1997
  • In order to observe the localization of the specific antigenic protein in the tissue of Paragonimus westermani metacercaria, immunogoldlabeling method was applied using IgG of the dog which were infected with Paragonimus westermani metacercaria and IgG of rabbits which were immunized with purified 23 kDa protein from metacercaria of the Paragenimus westermani. The metacercaria worm tissues obtained from Cambaroides similis were embedded in Lowicryl HM20 medium, treated with infected and immunized IgG and protein A gold complex (particle size; 12 nm) and observed by electron microscope. In the tissue antigen of Paragonimus westermani metacercaria, the content of excretory bladder which was highly dense electron density was constituted in the excretory bladder of the parenchymal tissue. In the metacercaria tissues antigen reacted with IgG of infected dog. Labeled gold particles distributed on the interstitial matrix of parenchymal cells, fibrous granules of parenchymal tissue and the content of excretory bladder. High antigenicity was observed on content of excretory bladder. It was found to be specifically distributed at the tissue of Paragonimus westermani metacercaria. In the tissues antigen reacted with IgG of immunized rabbit. Labeled gold particles randomly distributed on the interstitial matrix and fibrous granules of parenchymal tissue but in the content of excretory bladder of Paragonimus westermani metacercaria, gold particles were richly labeled. Therefore, the 23 kDa protein contained with Paragonimus westermani metacercaria was found protein which was specifically constituted at the content of excretory bladder of Paragonimum westermani metacercaria. The 23 kDa protein was commonly contained from of Paragonimus westermani metacercaria to adult and showed strong antigenicity against the immunized and infected IgG.

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Studies on the Infection Rate and Distribution Pattern of Metacercaria of Clonorchis sinensis in Pseudorasbora Parva (참붕어내 간흡충 피낭유충의 감염률 및 분포양상에 관한 연구)

  • Kim, Ki-Hong;Yie, Jae-Hyun;Joo, Kyoung-Hwan;Lee, Joon-Sang;Rim, Han-Jong
    • Journal of agricultural medicine and community health
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    • v.14 no.1
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    • pp.44-53
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    • 1989
  • The distribution pattern of Clonorchis sinensis metacercaria in Pseudorasvora parva population and correlation between P. parva and metacercaria of C. sinensis were studied. The surveved areas were Chomanpo and Bulamdong, Kim-Hae Gun which were endemic area of clonorchiasis, and wansa, Sa-Chon Gun, Souh Kyong-Sang Do. The results are as follows: 1) The areas of Chornanpo and Wansa showed high infectivity in 99-100% of infection rate and 282-308 of average infection number per-fish. But the area of Bulamdong showed relatively. low infectivity in 95.8% of infection rate and 44 of average infection number. 2) The distribution patterns of C. sinensis metacercaria in P. parva population which were collected in Chomanpo and Wansa were shown Poisson distribution and the distribution pattern in Bulamdong showed mid-pattern of shifting over from Poisson distribution to Negative binomial distribution. 3) The correlation between P. parva length and average number of C. sinensis metacercaria in the present studied areas represented as direct proportion relationship.

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Studies on Reproductive Ecology and Parasite of the Venus Clam, Cyclina sinensis, on the West Coast of Korea. 2. On the Metacercaria of Himasthla kusasigi Yamaguti, 1939 (Trematoda) found in the Venus Clam, Cyclina sinensis (한국 서해산 가무락조개, Cyclina sinensis의 번식생태 및 기생충에 관한 연구 2. 가무락조개에서 검출된 흡충류, Himasthla kusasigi Yamaguti, 1939에 대하여)

  • 김영길;정의영;김용호
    • The Korean Journal of Malacology
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    • v.16 no.1_2
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    • pp.43-48
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    • 2000
  • We investigated the effects of death and gametogenesis by infection of the trematode in the venus clam, Cyclina sinensis. The specimens of C. sinensis were collected monthly at the tidal flat of Kaehwado, Puan-gun and Sangpo aquafarms, Kochang-gun, Chollabuk-do, Korea, from June 1999 to May 2000. One species of a trematode, the metacercaria of Himasthla kusasigi Yamaguti, 1939 (Enchinistomatidae), was found in the venus clam, C. sinensis. Infection rates in Kaehwado area and Sangpo aquafarm were average 93% and 81%, respectively. Infection rates during the study period reached the maximum (100%) in September 1999 and March-April 2000 and showed the minimum (80%) in July in Kaehwado, while those in Sangpo aquafarm showed the maximum (99%) in January 2000 and the minimum (47%) in November 1999. The infection parts of the metacercaria of H. kusasigi in the venus clam were the visceral mass, gill, mantle including the foot. Their infection rates in Kaehwado area and Sangpo aquafarm were 76.7% and 81.3% in the visceral mass, 19.1% and 14.5% in the gill, and 5,1% and 4.2% in the mantle, respectively. Infection rates of H. kusasigi showed higher with the increase of the size of the clams. No abnormal characteristics in the various tissues by histological observations were found in the clams infected by the metacercaria of H. kusasigi because this clam is the second intermediate host.

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Changing patterns of infection with digenetic larval trematodes from Iresh-water fish in River Taewha, Kyongnam Province (경남 태화강에서 채집된 담수어에 있어서 흡충류 피낭유충 기생상의 변화)

  • 주종윤
    • Parasites, Hosts and Diseases
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    • v.26 no.4
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    • pp.263-274
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    • 1988
  • Recent patterns of infection with digenetic larval trematodes in fresh- water and brackish-water ask were studied in three locations of the river Taewha during the period from April to October, 1988, and compared with the data reported previously in the same river, 0(16 species of fish examined, the encysted larvae of Cyathocotyle orientalis were found most frequently from 9 species of fresh.water fish. The metacercariae of Echinochasmus sp. , Metacercaria hasegawai and Metagonimus yokogawai were found from 8 species, those of Clonorchis sinensis from 7 species, and Exorchis oviformis and Metorchis orientalis from 5 species of fish. The infection rates of fish with C. sinensis metacercariae were not lower than those reported in 1980, whereas their intensity of infection was found lowered in 3 species, Ccreobagrus brevicorpus, Gnathopogon atromaculatus, and Funtungia herzi. The infection rates of 3 species of fish with M. yokogawai metacercariae were lower than the results in 1982, while the rate was higher in 2 species, Zacco platypus, and Z. temmincki, and rather stationary in Flecoglossus altivelis. The intensity of infection in several species of fish appeared rather higher than in 1980. The encysted larvae of C. orientalis, Echinochasmus sp., E. oviformis and Metacercaria hasegawai showed variations in infection rates of fish in 1980 and in the present study. It was found that the rate of infection with digenetic larval trematodes in fresh- water fish was still relatively high in the river Taewha, and the metacercarial burden in the fish varied greatly by different fish in 1980 and in the present study.

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Studies on A Trematode Parasitized on Bivalves V. On metacercaria of Echinostomatidae detected from Mactra veneriformis, Cyclina sinensis and Solen strictus (조개류(類)에 기생(寄生)하는 흡충류(吸蟲類)에 관(關)한 연구(硏究) V. 동죽, 가무락조개 및 맛조개에서 검출(檢出)되는 극구흡충류(棘口吸蟲類)의 유충(幼蟲)에 대(對)하여)

  • Kim, Young-Gill
    • Journal of fish pathology
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    • v.1 no.1
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    • pp.31-37
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    • 1988
  • An experiment was carried out in order to reveal the infection species of trematode and the infection demage in the marine bivalves. Metacercaria of Echinostomatidae were found in Mactra veneriformis, Cyclina sinensis and Solen strictus in the vicinity sea of Naecho-do, the estuary of the Keum River in the western coast of Korea. The metacercaria detected from Solen strictus were fed by Larus crassiostris, at 16 days after infection, the adult worm could be seceded. The metacercaria of Himasthla kusasigi were detected from Mactra veneriformis and Cyclina sinensis, their infection rates were 70.4% and 85.7% respectively. The partial infection rates with respect to the body parts were 40.4% and 77.3% in the foot, 17.0% and 12.4% in the gill, 12.6% and 10.3% in the mantle in order. The metacercaria of Acanthoparyphium were found from Solen strictus. The total infection rate was 63% and the partial infection rate was 63% and the partial infection rate was shown foot, mantle and gill in order. The adult worm ceded from Larus crassiostris was indentified as Acanthoparyphium tyosenensis Yamaguti, 1938 from view point of its physical characteristics. Therefore, Mactra veneriformis, Cyclina sinensis would be added as new intermediate hosts, and Echoinostoma decteded from Mactra sulcataria which was studied by author(1969) was revealed as larvae of Acanthoparyphium tyosenensis.

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Experimental Studies on the Second Intermediate Host of Clonorchis sinensis I. Survey on the metacercariae of some trematodes in fresh-water shrimps and experimental infection of Palaemon spp. with Clonorchis cercariae (간흡충(肝吸蟲)의 제이중간숙주(第二中間宿主)에 관(關)한 연구(硏究) I. 민물 새우의 metacercaria 조사(調査) 및 간흡충(肝吸蟲) cercaria의 Palaemon spp.에 대(對)한 감염시험(感染試驗))

  • Rhee, Jae Ku
    • Korean Journal of Veterinary Research
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    • v.9 no.1
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    • pp.71-78
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    • 1969
  • The present studies were undertaken to determine accurately whether Korean native fresh-water shrimps can serve as the second intermediate host of Clonorchis sinensis. The experimental results obtained were as follows; 1. Metacercaria of Clonorchis sinensis was not discovered in 36 Macrobranchium nipponensis (De Hann), 1992 Palaemon paucidens (De Hann) and Palaemon madestus Heller, and 860 Caridina leucastica Stimpson, collected in Kimhae district, which were digested in artificial gastric juice, using binocular dissecting microscope. 2. Cercariae of the Chinese liver fluke could not approach the body wall of young shrimps due to streaming caused by their motion. 3. Young Palaemon spp. of 247 were divided into two groups and placed in two different room aquariums, one is at high temperature, the other is normal. Then they were contacted with the cercariae. Only 11 degenerated metacercariae were found in 9 Palaemon spp. It was suggested that they had died immediately or within a few days after penetration. Furthermore the temperature did not affect the infective ratio. 4. At the field experimental aquarium, no metacercaria of Clonorchis sinensis was discovered tn 100 Palaemon spp. which had been brought into contact with 5000 Parafossarulus manchouricus two trials. 5. Specific changes in the cercariae mixed with the extract of shrimps meat were not observed through the dissecting microscope. 6. These results suggest that the Korean native shrimps cannot serve as the intermediate host of Clonorchis sinensis.

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Infection of Clinostomum complanatum (Treamdoda: Digenea) metacercaria in cultured southern top mouthed minnow Pseudorasbora parva (참붕어, Pseudorasbora parva의 인두흡충, Clinostomum complanatum 피낭유충 감염증)

  • Jung, Eun-Bin;Kim, Dong-Wan;Kim, Jin-Do;Park, Sung-Woo
    • Journal of fish pathology
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    • v.25 no.2
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    • pp.85-93
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    • 2012
  • Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were $4.02{\pm}1.80$ mm (1.66-5.81) in length and $1.27{\pm}0.27$ mm (1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring $0.18{\pm}0.05$ mm (0.11-0.24) in length and $0.23{\pm}0.07$ mm (0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured $0.47{\pm}0.08$ mm (0.35-0.53) in length and $0.55{\pm}0.09$ mm (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.

Studies on the Life History of Bacciger harengulae (Bacciger harengulae의 생활사에 관한 연구)

  • KIM Young-Gill;CHUN Seh-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.5
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    • pp.449-470
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    • 1984
  • The cercaria of Bacciger herengulae which is parasitized on the gonad of Solen strictus was investigated in order to reveal its entire life history. The area covered for the study was in the vicinity sea of Naechodo, the estuary of the Kum river in the western coast of Korea during the period of 1980-1983. Morphology and development as well as infection rates of sporocyst and cercaria within Solen strictus were examined. For accomplishing the objectives of this study, an artificial infection experiment and some investigations on the second intermediate host, the final host and the growing stages were also studied in both laboratory and natural habitat of Solen strictus. According to the study, it was revealed that the first intermediate hosts were Meretrix lusoria, Solen strictus, Tapes japonica and Laternula limicola, the second intermediate host was Palaemon (Exopalaemon) carinicauda and the final hosts were Konosirus punctatus and Harengula zunasi. A mature sporocyst which was found in the gonad of Solen strictus was $4.0-4.3{\times}0.2-0.21\;mm$ insize, and the cercaia with 27 pairs of setae, each seta consisting of 6 tufts, was $270{\times}147{\mu}m$ in body size and $550{\times}52{\mu}m$ in tail size. Oral sucker($52{\times}42{\mu}m$), pharynx, vental sucker and two testese were obviously seen within the cercaria. The excretory vesicles of cercaria were in V-shape and the flame cell were formula was expressed as 2[(3+3)+(3+3)]=24. The infection of cercaria in the first intermediate host, Solen strictus, was found throughout the year regardlless of the water temperature, and its mean infection rate was $9.67\%$ during the study period. The infection rate fluctuated with temperature, the highest being $28.0\%\;at\;28.0^{\circ}C$ water temperature in July and the lowest $2.4\%\;at\;19.5^{\circ}C$ in October, and it increased in proportion to the shell length on the host. But cercaria was not detected at below 4.0 cm in size of the host. Mature cercariae were found 6 months from May to October when water temperature was above $19.5^{\circ}C$. On the other hand, when water temperature was below $19.5^{\circ}C$, only immature cercariae and sporocysts were found. The cercariae were active for 35 hours and survived for 71 hours at $20^{\circ}C$, and 29 and 34 hours at $25^{\circ}C$ respectively, whereas the cercariae were inactive at less than $20^{\circ}C$ in water temperature. Cercaria, from Solen strictus, approached shrimp of 1-3 cm in body length as its second host. Then, it began to intrude in to the muscle of shrimp after 2-3 hours. The infected cercaria formed cyst after 7-8 hours, and became mature metacercaria. $420{\times}310{\mu}m$ in size, 15 days afer infection. The infection rate of metaceria to shrimp in the laboratory was highest, at $25^{\circ}C$ being $61\%$ and at $20^{\circ}C\;17%$. The infection rate of metacearia in shrimp was highest in the first abdominal segment, followed by cephalothorax, the second, and fifth abdominal segments, and in that order. Also, the infection rate of metacercaria in wild shrimp was high $9.6-11.1\%$ at $26.5^{\circ}C$ in June, and low $1.56-2.5\%$ at $28-29.5^{\circ}C$ from July to August. The infected shrimp with metacercaria was experimentally fed to Konosirus punctatus in the laboratory in order to know its final host. The metacercaria developed into the adult worm, $440-520{\times}310-360{\mu}m$ in size, within the intestine of Konosirus punctatus 20 days after infection. The adult worm was oval shape and $20-24{\times}11-20{\mu}m$ in size. The infection rate of adult worm to Konosirus punctatus and Harengula zunasi ranged 87.3 to $100\%$, the mean being $95.2\%$, regardless of the body length of their hosts. The infection rate was $100\%$ in June and July, but it decreased in September and October. The size and body structure of the trematode observed during the present study were well agreed with those ievestigated by Yamaguti(1938), thus, it may be concluded that the adult worm it identified as Bacciger harengulae.

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Experimental Infection of Clonorchis sinensis to Cyprinus curpio nudes (간흡충의 이스라엘잉어(향어)에 대한 감염실험)

  • 이재구;김평길
    • Parasites, Hosts and Diseases
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    • v.21 no.1
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    • pp.11-18
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    • 1983
  • As a part of observation on the suitability as a second intermediate host of Clonorchis sinensis, various infection experiments were tried to Cyprinus carpio nodus. The results obtained were summarized as follows: 1. The cercariae which attempted to contact with Cyprinus carpio nodus in the water were observed under the stereomicroscope. After contact, cercariae began to separate its tails from the bodies at 4 minutes increasingly, and then the numbers ranged to 80% at 13 minutes after the encounter. But very few cercariae could actually invade into the epidermis of the fish. 2. When Cyprinus carpio nudes were exposed to a number of cercariae in the beaker, only a few cercariae could invade through the epidermis. Most of the invaded cercariae were killed before forming the cyst. Also rare encysted cercariae were found to be dead within 48 hours. 3. Cyprinus carpio nudes were reared with Parafossarulus manchouricus shedding numerous cercariae in the indoor aquarium. When observed after 12 days, to determine the susceptibility, no metacercaria was found infected. 4. Cyprinus carpio nudum were reared with Parafossarulus manchouricus shedding numerous cercariae in Kimhae lake for 2 months in summer of 1982, in order to check the natural infection. No metacercaria of Clonorchis sinensis was found from the fish. 5. In the cases of control fish, Pseudoraibora larva, numerous cercariae were inspected on the course of invasion through epidermis of the fish under the stereomicroscope. And many metacercariae were also found from the Ssh whenever they were kept in beaker, indoor aquarium, or the lake for natural infection. The results suggested that Cyprinus carpio kudus might not be proper intermediate host of Clonorchis sinensis.

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Effects of gamma-irradiation on the survival and development of Metagonimus yokogawai metacercariae in rats (감마선 조사가 요코가와흡충의 흰쥐 내 생존 및 발육에 미치는 영향)

  • 채종일;김상준
    • Parasites, Hosts and Diseases
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    • v.33 no.4
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    • pp.297-304
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    • 1995
  • To evaluate the feasibility of irradiation as a control measure for metagonimiasis, the metacercariae of Metagonimus yokogcwni were irradiated with gamma ray, either after isolation from the sweetfish (Plecoglossus cltivelis) or in situ of the fish, and their survival and development in rats were observed at 7 days post-infection. The radiation dose varied from 5 to 100 Gy for the metacercaria-irradiation group and from 5 to 500 Gy for fish-irradiation group. The results showed that the worm recovery rate from the irradiation groups decreased as the radiation dose was increased. Higher doses of radiation were required for the fish-irradiation group to obtain the same results as the metacercaria-irradiation group. The LD50 of the metacercaria-irradiation group was 4.5 Gy, whereas that of the fish-irradiation group 6.2 Gy A few number of worms which survived until 7 days in rats were severely retarded especially in the growth of their reproductive organs, j.e., complete or partial failure in the development of testes and formation of uterine eggs . The present study revealed that irradiation of sweetfish by 200 Gy is effective to control infectivity as well as development of M. vokogawai metacercariae in rats.

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