• Journal of Chest Surgery
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• v.17 no.1
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• pp.101-109
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• 1984

The influences of acute respiratory and metabolic acid-base disturbances on the carotid, renal and coronary blood flow were measured in dogs. Respiratory acidosis was induced by artificial respiration with 8% CO2 -02 gas mixture and respiratory alkalosis was induced by hyperventilation under the control of respirator. Metabolic acidosis and metabolic alkalosis were induced by intravenous infusion of 0.3N hydrochloric acid and 0.6M sodium bicarbonate solution. To observe the effect of hyperkalemia, isotonic potassium chloride solution was infused. CVI electromagnetic flowmeter probes were placed on the left common carotid artery, left renal artery and left circumflex coronary artery. Each flow was recorded on polygraph. 1. The carotid blood flow showed rapid showed rapid and marked increase in acute respiratory acidosis. Even in the cases when arterial blood pressure was lowered during the state of respiratory acidosis, carotid blood flow increased. By the infusion of hydrochloric acid, carotid blood flow increased slowly and returned to the previous label after discontinuation of the infusion. Carotid blood flow also increased by the infusion of large amount of sodium bicarbonate, but it might be the combined effect of expansion of extracellular fluid and compensatory elevation of carbon dioxide tension. 2.The renal blood flow remained unchanged during the acute acid-base disturbances, suggesting effective autoregulation. Renal blood flow, however, increased very slowly when the infusion of potassium chloride continued for a long period. 3.Although less marked than the carotid blood flow, the coronary blood flow increased in the acute respiratory and metabolic acidosis. In asphyxiated condition, coronary blood flow increased most markedly and this might be the combined effect of hypoxia, hypercapnea, and lowering of pH. In summary, the carotid blowflow showed more marked change in the acute respiratory and metabolic acidosis than the renal and coronary blood flow. Respiratory and metabolic components of acid-base disturbances may influence the local blood flow concomitantly, there being more differences in the individual responses, but respiratory component manifested more rapid and marked effect than metabolic component.

• The Korean Journal of Zoology
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• v.31 no.2
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• pp.81-86
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• 1988

The relationship between cumulus cell expansion, cocyte maturation and metabolic cooperativitiy was investigated by using mouse and pig cocyte-cumulus complexes in vitro. Cocyte germinal vesicle breakdown (GVBD) and cumulus expansion were manipulated with hormones or reagents which increase intracellular cAMP leveL Metabolic cooperativity between oocyte and cumulus cells was assessed by determination of the fraction of radiolabelled uridine marker that was transferred from the cumulus mass to the oocyte. Uptake of uddine marker by mouse and pig cumulus mass was increased by about fourfold of basal level with the stimulation of hormones (human choriononic gonadotrophin, HCG; follicle stimulating hormone, FSH) or cyclic AMP sttmulators (3-isobutyl-1-methylxanthine, IBMX; forskolin) during culture. However, the fraction of uridine that was transferred from the cumulus mass to the cocyte (transfer ratio) was gradually decreased during culture, irrespective with the presence of hormones or stimulators. The decrease of the transfer ratio was not correlated with the state of occyte whether they have GV or not, or with the degree of cumulus expansion. In mouse complexes, HCG induced more significant reducton of transfer ratio than other treatments. These results do not support the idea that modulations of metabolic cooperativity between cumulus cells and oocytes are important for the regulation of meiotic resumption in mammals.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1163-1169
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• 2004

An unstructured mathematical model is presented for lactic acid fermentation based on the energy balance. The proposed model reflects the energy metabolic state and then predicts the cell growth, lactic acid production, and glucose consumption rates by relating the above rates with the energy metabolic rate. Fermentation experiments were conducted under various initial lactic acid concentrations of 0, 30, 50, 70, and 90 g/l. Also, a genetic algorithm was used for further optimization of the model parameters and included the operations of coding, initialization, hybridization, mutation, decoding, fitness calculation, selection, and reproduction exerted on individuals (or chromosomes) in a population. The simulation results showed a good fit between the model prediction and the experimental data. The genetic algorithm proved to be useful for model parameter optimization, suggesting wider applications in the field of biological engineering.

• Bulletin of the Korean Chemical Society
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• v.11 no.6
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• pp.557-560
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• 1990

The transition from one stable steady state branch to another stable steady state branch in a simple metabolic system with positive feedback is discussed with the aid of the bimodal Gaussian probability distribution method. Fluctuations lead to transitions from one stable steady state branch to the other, so that the bimodal Gaussian evolves to a new distribution. We also obtain the fractional occupancies in the two stable steady states in terms of a parameter characterizing conditions of the system.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.515-518
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• 2003

Six mature purebred Angus cows ($3.5{\pm}1.5$ years of age, $631.36{\pm}8.63kg$), three pregnant ($250{\pm}7$ days pregnant) and three non-pregnant and eight mature Suffolk ewes ($5{\pm}1.2$ years of age, $68.18{\pm}2.3kg$) four pregnant $115{\pm}5$ days pregnant) and four nonpregnant were utilized in a five-day metabolic trial to determine the effects of gestational status (pregnant vs. non-pregnant) on apparent absorption and retention of copper (Cu) and zinc (Zn). Animals were selected based on body weight, age, and gestational status, and randomly assigned to metabolic crates for total fecal and urine collection. Animals were allowed to acclimate to their new environment for seven days. Pregnant and non-pregnant cows and ewes were then paired (within a species) by body weight and pair-fed throughout the 5 day collection period. Copper and Zn intakes were similar for pregnant and non-pregnant animals within a species. Apparent absorption of Cu (p<0.06) and Zn (p<0.04) were higher in pregnant cows relative to non-pregnant cows. Pregnant cows also had a higher apparent retention of Cu (p<0.05) and Zn (p<0.06) relative to non-pregnant cows. Pregnant ewes had a higher (p<0.01) apparent absorption and retention of Zn compared to non-pregnant ewes. However, apparent absorption and retention of Cu were similar for pregnant and non-pregnant ewes. These data indicate that certain physiological and/or metabolic parameters are altered in pregnant cows and ewes consuming an alfalfa-based diet that enhance the apparent absorption and retention of certain trace minerals.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.311-314
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• 2003

Under hyperosmotic stress, rCHO cells display decreased specific growth rate $({\mu})$ and increased specific antibody productivity $(q_{Ab})$. The effects of hyperosmotic stress on batch culture cellular dynamics are not well understood. To this end, we conducted a proteome profile of rCHO cells, using 2D-gel, MALDI-TOF-MS and MS/MS. As a result, the proteome profile of rCHO cells could be established using 41 identified proteins. Based on this proteome profile of rCHO cells, we have found at least 8 differently expressed spots at hyperosmotic osmolality (450 mOsm/kg). Among these spots, two metabolic enzymes were found to be up-regulated (pyruvate kinase and GAPDH), while down-regulated protein was identified as tubulin. It shows that hyperosmotic stress can alter metabolic state, by up-regulated activities of two glycolysis enzymes, which could lead to activate the generation of metabolic energy. Tubulin expression was down-regulated, suggesting a reduction of cell division. Finally, the increased conversion energy could leads to improve overall productivity.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.1002-1008
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• 1999

Essential metabolic functions have been identified for seven macrominerals (calcirum, phosprorus, magnesium, sodium, potassium, chloride, and sulfur), and eight microminerals (cobalt, copper, iodine, iron, manganese, molybdenum, selenium, and zinc). Major functions for each of these minerals are summarized. Considerable research suggests that chromium is also essential and that it functions by facilitating insulin activity. Studies are reviewed which indicate that chromium supplementation of animal diets may: 1) increase glucose removal from blood, 2) reduce carcass fat and increase lean in nonruminants, 3) alter egg cholesterol content, and 4) enhance immunity and disease resistance in ruminants. A number of other minerals including nickel, boron, vanadium, arsenic, silicon, lithum, and lead have been reported to be essential, but specific metabolic functions have not been defined for any of these elements. Limited research in poultry suggests that boron may be of practical significance in some instances.

• Molecules and Cells
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• v.39 no.12
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• pp.847-854
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• 2016

The early landmark discoveries in cancer metabolism research have uncovered metabolic processes that support rapid proliferation, such as aerobic glycolysis (Warburg effect), glutaminolysis, and increased nucleotide biosynthesis. However, there are limitations to the effectiveness of specifically targeting the metabolic processes which support rapid proliferation. First, as other normal proliferative tissues also share similar metabolic features, they may also be affected by such treatments. Secondly, targeting proliferative metabolism may only target the highly proliferating "bulk tumor" cells and not the slowergrowing, clinically relevant cancer stem cell subpopulations which may be required for an effective cure. An emerging body of research indicates that altered metabolism plays key roles in supporting proliferation-independent functions of cancer such as cell survival within the ischemic and acidic tumor microenvironment, immune system evasion, and maintenance of the cancer stem cell state. As these aspects of cancer cell metabolism are critical for tumor maintenance yet are less likely to be relevant in normal cells, they represent attractive targets for cancer therapy.

• Nutritional Sciences
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• v.2 no.2
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• pp.113-118
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• 1999

Critical evaluation of energy intake data from dietary studies is difficult but important. To investigate the underreporting of total energy intake, we analyzed the one-day dietary intake data collected by 24-hour recall method from 550 elderly Koreans aged 60 years or older. Underreporting was addressed by computing the ratio of energy intake (EI) to estimated basal metabolic rate (BMRest). EI : BMRest ratio was found to be 1.38 for, men and 1.33 for women, with about 14% of men and women classified as underreporters. Underreporting of energy intake was highest in men and women who were overweight, had lower family income, or no school education. For men, the most significant variables to predict the ratio of energy intake to estimated basal metabolic. rate (EI : BMRest) were weight status, members of household, alcohol consumption and age, while income and education level were most significant for women.

• Journal of Korean Public Health Nursing
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• v.26 no.3
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• pp.417-427
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• 2012

Purpose: The purpose of this study was to examine the effect of a 12-week combined exercise training program on the body composition, physical fitness levels, and metabolic syndrome profiles of obese women. Methods: Twelve obese women were assigned to the combined exercise training program group. The women underwent training for 70-90 min/d, three times per week for a period of 12 weeks. Paired samples t-tests were performed using SPSS ver. 17.0 for analysis of the results. Results: The results of this study showed that body-composition parameters such as weight, fat-free mass, body fat mass, body-mass index, body fat, waist-hip ratio, basal metabolic rate, and intra-abdominal fat, physical fitness parameters such as muscle strength, muscle endurance, flexibility, and cardiac endurance, and metabolic syndrome biomarkers such as triglyceride levels, high-density lipoprotein cholesterol levels, glucose levels, systolic blood pressure, and waist circumference before participation the training program differed significantly from those after participation in the training program (p<0.05). However, diastolic blood pressure before participation in the training program did not differ significantly from that after participation in the training program (p>0.05). Conclusion: We concluded that a 12-week combined exercise training program could be a good exercise program for improvement of the body composition, physical fitness levels, and metabolic syndrome profiles of obese women.