• KSBB Journal
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• v.29 no.3
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• pp.165-178
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• 2014

Statistical medium optimization has been carried out for the production of succinic acid in anaerobic fermentations of Actinobacillus succinogenes. Succinic acid utilized as a precursor of many industrially important chemicals is a fourcarbon dicarboxylic acid, biosynthesized as one of the fermentation products of anaerobic metabolism by A. succinogenes. Through OFAT (one factor at a time) experiments, corn steep liquor (CSL), a very cheap agricultural byproduct, was found to have significant effects on enhanced production of succinic acid, when supplemented along with yeast extract. Hence, using these factors including glucose as a carbon/energy source, interactive effects were investigated through $2^n$ full factorial design (FFD) experiments, showing that the concentration of each component (i.e., glucose, yeast extract and CSL) should be higher. Further statistical experiments were conducted along the steepest ascent path, followed by response surface method (RSM) in order to find out optimal concentrations of each constituent. Consequently, optimized concentrations of glucose, yeast extract and CSL were observed to be 180 g/L, 15.08 g/L and 20.75 g/L respectively (10 g/L of $NaHCO_3$ and 100 g/L of $MgCO_3$ to be supplemented as bicarbonate suppliers), with the estimated production level of succinic acid to be 92.9 g/L (about 3.5 fold higher productivity as compared to the initial medium). Notably, the RSM-estimated production level was almost similar to the amount of succinic acid (92.9 g/L vs. 89.1 g/L) produced through the actual fermentation process performed using the statistically optimized production medium.

• KSBB Journal
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• v.14 no.2
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• pp.255-258
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• 1999

Effects of growth conditions on the growth of Zoogloea ramigera and the production of zooglan were investigated. The production of zooglan was greatly reduced in the phosphate-limiting medium. $NH_4Cl$ and ${(NH_4)}_2SO_4$ improved cell growth when they were used as a nitrogen source. The medium containing 45 g/L of glucose and 27 g/L of $NaNO_3$ resulted in the highest production of zooglan at 18.5 g/L.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.401-406
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• 1994

The medium compositions of Alcaligenes eutrophus were optimized for increasing PHB productivity. It is very important to optimize the concentrations of inorganic salts and trace eleme- nts as well as carbon and nitrogen sources to maximize cell growth rate and productivity. The fed-batch culture of Alcaligenes eutrophus by dual feeding of ammonia water and glucose under optimized initial medium concentrations was carried out. Glucose was fed manually according to glucose consumption rate and ammonia water by pH-stat. The final cell concentrations and PHB content in 30 hours were 122 g/l and 65% of dry cell weight(yielding 79 g of PHB/l), respectively and 2.64 g/l/hr of PHB production rate was obtained.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.57-64
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• 2000

The present work investigates the heat transfer characteristics for laminar fully developed forced convection in an internally finned tube with axially uniform heat flux and peripherally uniform temperature through analytical models of convection in a porous medium. Using the Brinkman-extended Darcy flow model and the two equation model fur heat transfer, analytical solutions fur fluid flow and heat transfer are obtained and compared with the exact solution for fluid flow and the numerical solutions for conjugate heat transfer to validate the porous medium approach. Using the analytical solutions, parameters of engineering importance are identified and their effects on fluid flow and heat transfer are studied. Also, the expression fur total thermal resistance is derived from the analytical solutions and minimized in order to optimize the thermal performance of the internally finned tubes.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1932-1937
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• 2008

The R-amidase production by a newly isolated strain of Delftia tsuruhatensis ZJB-05174 was optimized in this paper. Effects of factors such as carbon sources, nitrogen sources, and inducers on amidase production were investigated. The medium composition was optimized using central composite designs and response surface analysis. The optimal medium components for enhanced amidase production were found to be as follows: glucose, 8.23 g/l; yeast extract, 11.59 g/l; 2,2-(R,S)-dimethylcyclopropane carboxamide, 1.76 g/l; NaCl, 1 g/l; ${KH_2}{PO_4}$ 1 g/l; and ${K_2}{HPO_4}$ 1 g/l. A maximum enzyme production of 528.21 U/l was obtained under the optimized conditions, which was 4.7 times higher than that obtained under initial conditions.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.717-725
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• 2021

This study aimed to optimize medium composition and culture conditions for enhancing the biomass of Lactobacillus plantarum 200655 using statistical methods. The one-factor-at-a-time (OFAT) method was used to screen the six carbon sources (glucose, sucrose, maltose, fructose, lactose, and galactose) and six nitrogen sources (peptone, tryptone, soytone, yeast extract, beef extract, and malt extract). Based on the OFAT results, six factors were selected for the Plackett-Burman design (PBD) to evaluate whether the variables had significant effects on the biomass. Maltose, yeast extract, and soytone were assessed as critical factors and therefore applied to response surface methodology (RSM). The optimal medium composition by RSM was composed of 31.29 g/l maltose, 30.27 g/l yeast extract, 39.43 g/l soytone, 5 g/l sodium acetate, 2 g/l K₂HPO₄, 1 g/l Tween 80, 0.1 g/l MgSO₄·7H₂O, and 0.05 g/l MnSO₄·H₂O, and the maximum biomass was predicted to be 3.951 g/l. Under the optimized medium, the biomass of L. plantarum 200655 was 3.845 g/l, which was similar to the predicted value and 1.58-fold higher than that of the unoptimized medium (2.429 g/l). Furthermore, the biomass increased to 4.505 g/l under optimized cultivation conditions. For lab-scale bioreactor validation, batch fermentation was conducted with a 5-L bioreactor containing 3.5 L of optimized medium. As a result, the highest yield of biomass (5.866 g/l) was obtained after 18 h of incubation at 30℃, pH 6.5, and 200 rpm. In conclusion, mass production by L. plantarum 200655 could be enhanced to obtain higher yields than that in MRS medium

• Journal of Applied Reliability
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• v.18 no.3
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• pp.229-239
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• 2018

Purpose: There is a difficulty in Environmental Stress Screening (ESS) test design for weapon system's electrical/electronic components/products in small and medium-sized enterprises. To overcome this difficulty, I propose an easy ESS test design approach algorithm that is optimized with only one environment tolerance design information parameter (${\Delta}T$). Methods: To propose the mass production weapon system ESS test design for cost-effective optimization, I define an optimum cost-effective mathematical model ESS test algorithm model based on modified MIL-HDBK-344, MIL-HDBK-2164 and DTIC Technical Report 2477. Results: I clearly confirmed and obtained the quantitative data of ESS effectiveness and cost optimization along our ESS test design algorithm through the practical case. I will expect that proposed ESS test method is used for ESS process improvement activity and cost cutting of mass production weapon system manufacturing cost in small and medium-sized enterprises. Conclusion: In order to compare the effectiveness of the proposed algorithm, I compared the effectiveness of the existing ESS test and the proposed algorithm ESS test based on the existing weapon system circuit card assembly for signal processing. As a result of the comparison, it was confirmed that the test time was reduced from 573.0 minutes to 517.2minutes (9.74% less than existing test time).

• Steel and Composite Structures
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• v.51 no.6
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• pp.647-659
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• 2024

Given the increased interest in enhancing structural sustainability, the current study sought to apply multiobjective optimization to a footbridge with a steel-concrete composite I-girder structure. It was considered as objectives minimizing the cost for building the structure, the environmental impact assessed by CO2 emissions, and the vertical accelerations created by human-induced vibrations, with the goal of ensuring pedestrian comfort. Spans ranging from 15 to 25 meters were investigated. The resistance of the slab's concrete, the thickness of the slab, the dimensions of the welded steel I-profile, and the composite beam interaction degree were all evaluated as design variables. The optimization problem was handled using the Multiobjective Harmony Search (MOHS) metaheuristic algorithm. The optimization results were used to generate a Pareto front for each span, allowing us to assess the correlations between different objectives. By evaluating the values of design variables in relation to different levels of pedestrian comfort, it was identified optimal values that can be employed as a starting point in predimensioning of the type of structure analyzed. Based on the findings analysis, it is possible to highlight the relationship between the structure's cost and CO2 emission objectives, indicating that cost-effective solutions are also environmentally efficient. Pedestrian comfort improvement is especially feasible in smaller spans and from a medium to a maximum level of comfort, but it becomes expensive for larger spans or for increasing comfort from minimum to medium level.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.207-209
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• 2005

Serum is a potential source of bacterial, mycoplasmal and viral contamination, and it has a possibility of the introduction of serum proteins, prion and pyrogens into the final vaccine product. For porcine Rotavirus vaccine production, it is necessary to develop serum free medium which do not cause those problems. A new serum free medium was developed for porcine Rotavirus vaccine based on DMEM, and the performance of developed serum free medium was evaluated in terms of Vero cell growth and Rotavirus vaccine production. The cell density, gown in serum free medium developed, was similar with that in serum supplemented medium. Also, it was higher than that in other commercially available serum free medium. The productivity of Rotavirus vaccine using serum free medium developed and optimum production strategies will be also discussed.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.153-156
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• 2003

The MRS medium is widely used as an optimized medium for the growth of Lactobacillus spp. and also used for the growth of Leuconostoc spp. Leuconostoc mesenteroides shows quite different physicochemical properties compared to Lactobacilli spp. and it is one of the major strain of kimchi fermenting microorganisms with its usefulness in our traditional foods and availability in biotechnology in the future, specifically tailor-made medium is necessary for the growth of Leuconostoc mesenteroides. Sequential experimental designs (Plackett-Burman, fractional factorial, steepest ascent, central composite design and response surface methodology) were introduced to optimize and improve the Leuconostoc medium. Fifteen medium ingredients were investigated and fructose, sodium acetate and ammonium citrate were determined to give a critical and positive effect for cell-growth. The yield of biomass using the optimal medium was improved more than that of the MRS medium and the result of fed-batch culture showed the capability of the improvement in cell mass similar to the E.coli system.