• Title/Summary/Keyword: medium condition

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Studies on Aseptic Culture of Seed in Dendrobium Monile I. Effects of Basal Media and Growth Regulators on Germination of Seeds and Growth of Plantlet (한약자원식물인 Dendrobium monile 종자의 무균배양에 관한 연구 I. 기본배지 및 생장조절 생질이 종자의 발아와 유묘 생육에 미치는 영향 ( Studies on Aseptic Culture of Seed in Dendrobium Monile I. Effect of Basal Media and Growth Regulators on Germination of Se)

  • 최성규
    • Korean Journal of Plant Resources
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    • v.1 no.1
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    • pp.88-92
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    • 1988
  • The study was conducted to determine the optimal basal media and the concentration of plant growth regulator for germination of seeds and growth of plantlet from Dendrobium monile. The results obtained were summarized as follows. Germination was similar in light and dark condition, but the growth of plantlet after germination was better under dark than under light condition in several media. Germination was best in Hyponex and Kyoto solution medium among the 9 media tested. The number of roots/shoot was most in the Hyponex medium containing 0,1ppm NAA and 1.Oppm BA.

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Servo Track Writing for Ultra-High TPI Disk Drive in Low Density Medium Condition (초고밀도 디스크 드라이브를 위한 저밀도 작동 환경에서 서보 트랙 기록 방법에 대한 연구)

  • 한윤식;김철순;강성우
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2004.05a
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    • pp.736-741
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    • 2004
  • In high-capacity disk drives with ever-growing track density, the allowable level of position error signal (PES) is becoming smaller and smaller. In order to achieve the high TPI disk drive, it is necessary to improve the writing accuracy during the STW(servo track writing) process through the reduction of TMR sources. Among the main contributors of the NRRO(Non-Repeatable Run-out) PES, the disk vibration and the HSA(head-stack assembly) vibration is considered to be one of the most significant factors. Also the most contributors of RRO(Repeatable Run- out) come from the contributors of NRRO which is written-in at the time of STW(servo track writing) process. In this paper, the experimental test result shows that the effect of NRRO on servo written-in RRO effectively can be reduced through a STW process under low dense medium condition such as semi-vacuum.

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Phytomitogen Induced Blast Transformation of Guinea Pig : I. Effect of Medium, Phytomitogen and Cell Concentration on the Uptake of 3H-Thymidine (Phytomitogen에 의한 기니픽 임파구의 Blast Transformation : I. 유사분열에 미치는 배지, 유사분열촉진물질 및 세포농도의 효과)

  • Kim, Jong-shu;Bak, Ung-bok
    • Korean Journal of Veterinary Research
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    • v.26 no.2
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    • pp.245-249
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    • 1986
  • The present study has been carried out to investigate the optimal condition on the blastogenesis of guinea pig lymphocytes. A microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vito optimal condition of guinea pig lymphocytes. Data were presented to show many variables that are Involved in studying the responses of guinea pig lymphocyte in a microculture system to the stimulation of Concanavalin A(Con A) and lipopolysaccharide (LPS). Analysis indicated that the conditions for optimal Con A as measured by incorporation of $^3H$-TdR include : (1) use of RPMI-1640 as culture medium, (2) use of $6{\mu}g$ of Con A, per culture, (3) use of $1{\times}10^6$ cells per culture. Conditions for optimal stimulation with LPS mitogen were similar to those used for Con A.

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Phytomitogen induced blast transformation of rabbit I. Effect of medium, phytomitogen and culture hours on the uptake of 3H-thymidine (Phytomitogen에 의한 토끼 임파구의 blast transformation I. 유사분열에 미치는 배지, 유사분열촉진물질 및 배양시간의 효과)

  • Kim, Jong-shu;Kim, Chung-hui
    • Korean Journal of Veterinary Research
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    • v.31 no.1
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    • pp.49-53
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    • 1991
  • The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the In vitro optimal condition of rabbit lymphocytes. Data were presented to show many variables that are involved in studying the phytohemagglutinin(PHA) and lipopolysaccharide(LPS) response of rabbit lymphocyte in a microculture system. Analysis indicated that the conditions for optimal PHA as measured by incorporation of $^3H$-TdR include: (1) use of RPMI-1640 as culture medium. (2) use of $6{\mu}g$ of PHA, per culture. (3) 48-hours culture period. Conditions for optimal stimulation with LPS mitogen were similar to those used for PHA.

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Degradation oof Triphenylmrthane Dyes by Citobacter sp. (Citrobacter sp.에 의한 Triphenylmethane계 색소의 분해)

  • 민상기;조영배;전홍기
    • Journal of Life Science
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    • v.5 no.1
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    • pp.8-19
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    • 1995
  • The Optimal condition for degradation of crystal violet and other triphenylmethane dyes by Citrobacter sp. SK-3 isolated from the activated sludge of dye manufacturing factory was investigated. The optimal culture medium for the degradation of triphenylmethane dye was composed of minimum inorganic salt medium supplemented with 0.5% galactose, 0.1% beef extract, with the initial pH of 8.0 to 9.0. Under this condition, Citrobacter sp. SK-3 degraded 200 ppm of crystal violet completely within 24 hours. Citrobactre sp. SK-3 also degraded efficiently malachite green, pararosaniline, brilliant green, methyl violet, basic fuchsin and methyl red. Analysis of the degradation products of crystal violet through this layer chromatography and high performance liquid chromatography indicated that the methyl groups bound to crystal violet backborn were gradually demethylated to pentamethyl-, tetramethyl- and trimethylpararosaniline.

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A Empirical Study on University Students' Perception and Condition of Information (대학생들의 정보화 인식과 실태에 관한 실증적 연구)

  • 최락인
    • Journal of the Korea Society of Computer and Information
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    • v.6 no.4
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    • pp.179-187
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    • 2001
  • The purpose of this study is to survey university students' perception and condition of information and then, indicate the problems and suggest their solutions. As a result, it was found that supply ratio. insufficient using a medium of information, excessive cost, and depending on a private institute This study suggested the information equipment should be supplied. to develop the effective programs. controling unnecessary using a medium of information. the role of university in information education.

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Optimized Shoot Induction and Histological Study of in vitro Cultured Korean Soybean Cultivars

  • Kantayos, Vipada;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.32 no.3
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    • pp.237-243
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    • 2019
  • Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, 'Dawon', 'Pungsan', 'Daewon', 'Taekwang' and 'Chongdoo 1' by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, 'Dawon' and 'Pungsan'. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 mg/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in 'Dawon' cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.

A study on performance improvement of ultrasonic ranging system (초음파 거리계의 성능 개선에 관한 연구)

  • 박종건;임영철;조경영;박철수;김영민
    • 제어로봇시스템학회:학술대회논문집
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    • 1991.10a
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    • pp.1209-1213
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    • 1991
  • This paper describes a new method for performance improvement ultrasonic ranging system using V40 controller. Detectable range of ultrasonic ranging system depends on transducer frequency and bandwidth of the receiver, and damping of the transducer, etc. Conventional ranging systems are somewhat inaccurate because they do not have capability of compensating changes in medium condition. The novel ranging system overcomes this disadvantage by placing in the same medium a dummy sensor functioning as a standard calibrating instrument and by providing data processing capability using V40 controller.

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A Study on the Fatigue Crack Propagation of Partly Heat Treated Medium Carbon Alloy Steel (부분 열처리한 기계 구조용 합금강의 피로균열 전파에 관한 연구)

  • 이억섭;김선용
    • Journal of the Korean Society of Safety
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    • v.12 no.1
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    • pp.3-8
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    • 1997
  • It is well known that mechanisms of fracture and fatigue crack propagation depend upon various characteristics such as environmental condition, crack geometry, heat treatment and mechanical properties. It seems to be important for the detailed evaluation of structural components which contain flaws. In this paper, it is studied that the fatigue crack propagation of partly heat treated medium carbon alloy steel(SCM440) by high frequency heat treatment.

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Plant Regeneration from Mesophyll Protoplast of Nicotiana africana (Nicotiana africana의 엽육 원형질체로부터 식물체 재분화)

  • 최상주;이승철
    • Journal of the Korean Society of Tobacco Science
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    • v.15 no.2
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    • pp.130-136
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    • 1993
  • Protoplasts of palisade cells were aseptically isolated from leaves of Nicotiono apicona Merxm. by the one step enzymatic method. Efficiency of colony formation were depended on cell density and light condition during incubation, but an intensity of 47 ft-c during a period of 2 weeks after isolation of the protoplasts in the Nagata and Takebe's medium promoted the planting efficiency. Protoplast - derived calli of N. africana can be differentiated into shoot when cultured on Murashige and skoog's medium containing IAA(0.Smg/$\ell$) and zeatin(5.0mg/$\ell$).

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