Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Optimized Shoot Induction and Histological Study of in vitro Cultured Korean Soybean Cultivars

  • Kantayos, Vipada (Department of Well-being Resources, Sunchon National University) ;
  • Bae, Chang-Hyu (Department of Well-being Resources, Sunchon National University)
  • Received : 2019.06.06
  • Accepted : 2019.06.13
  • Published : 2019.06.30
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Abstract

Soybean is the one of recalcitrant legume species for shoot induction. Shoot regeneration via direct organogenesis was investigated in five soybean cultivars, 'Dawon', 'Pungsan', 'Daewon', 'Taekwang' and 'Chongdoo 1' by using cotyledonary node explants. Out of 5 soybean cultivars, an efficient shoot regeneration condition was developed in the two soybean cultivars, 'Dawon' and 'Pungsan'. When various kinds of plant growth regulators with different concentration were estimated, the optimum medium condition for shoot induction in both soybean cultivars was MS + B5 vitamin supplemented with BA at concentration 2 mg/L. In addition, shoot formation efficiency was increased with 97.09% and 93.88% by the pretreatment of BA onto the explants before in vitro culture in both cultivars. Shoot induction in 'Dawon' cultivar was originated from epidermal tissue and sub-epidermal layers when histological changes were investigated under shoot regeneration after culturing cotyledonary node segments on shoot induction medium for 0 to 21 days. Especially, cell dedifferentiation was observed from parenchyma cells to meristematic cell in 3-day cultured segments.

Keywords

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Fig. 1. Types of explant used in this experiment. Cotyledon (A), cotyledonary node (B) and hypocotyl (C) from Glycine max L. cv. ‘Dawon’.

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Fig. 2. Two kinds of cotyledonary node segments. Explants with cotyledonary node (A) and half-split cotyledonary node (B) from Glycine max L. cv. ‘Dawon’, respectively.

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Fig. 3. Response of three-kind explants on shoot induction media (MS+BA 2 ㎎/L) in Glycine max L. cv. ‘Dawon’. Cotyledon segment (A), Cotyledonary node segment (B) and Hypocotyl segment (C, arrow point showed callus induced from the segment).

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Fig. 4. Histological analysis of the shoot induction process in Glycine max L. cv. ‘Dawon’.

Table 1. Shoot induction from cotyledonary node explant of Glycine max L. 5 cultivars on shoot induction media supplemented with different concentration and kinds of plant growth regulators after 3 weeks

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Table 2. Effect of pretreatment with high concentration of BA on percentage of shoot induction in half-split cotyledonary node and cotyledonary node explants after 21 days cultures in soybean cultivars (Glycine max L.)z

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References

  1. Bailey, M.A., H.R. Boerma and W.A. Parrott. 1993. Genotype effects on proliferative embryogenesis and plant regeneration of soybean. In Vitro Cell. Dev. Biol. 29P:102-108.
  2. Choi, H., J.C. Yang, S.H. Ryu, S.M. Yoon, S.Y. Kim and S.Y. Lee. 2019. In vitro multiplication of Hosta Tratt. species native to Korea by shoot-tip culture. Korean J. Plant Res. 32:53-62 (in Korean). https://doi.org/10.7732/KJPR.2019.32.1.053
  3. Compton, M.E. and D.J Gray. 1993. Somatic embryogenesis and plant regeneration from immature cotyledons of watermelon. Plant Cell Rep. 12:61-65. https://doi.org/10.1007/BF00241935
  4. Dibax, R., G.B. Alcantara, M.P. Machado, J.C.B. Filho and R.A. Oliveira. 2013. Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB 93509 sugarcane cultivars. Ciencia Rural, Santa Maria 43(1):49-54.
  5. Franklin, C.I., T.N. Trieu, B.G. Cassidy, R.A. Dixon and R.S. Nelson. 1993. Genetic transformation of green bean callus via Agrobacterium mediated DNA transfer. Plant Cell Rep. 12:74-79. https://doi.org/10.1007/BF00241938
  6. Franklin, G., L. Carpenter, E. Davis, C.S. Reddy, D. Al-Abed, W. Abou Alaiwi, M. Parani, B. Smith, S.L. Goldman and R.V. Sairam. 2004. Factors influencing regeneration of soybean from mature and immature cotyledons. Plant Growth Regul. 43:73-79. https://doi.org/10.1023/B:GROW.0000038359.86756.18
  7. Hong, H.P., H. Zhang, P. Olhoft, S. Hill, H. Wiley, E. Toren, H. Hillebrand, T. Jones and M. Cheng. 2006. Organogenic callus as the target for plant regeneration and transformation via Agrobacterium in soybean (Glycine max (L.) Merr.). In Vitro Dev-Pl. 43(6):558-568.
  8. Jackson, J.A. and S.L.A. Hobbs. 1990. Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.). In Vitro Cell Dev-Pl. 26:825-835.
  9. Kantayos, V. 2019. Characterization of plantlet regeneration and genetic transformation of legume plants. School of Plant Production Science, Ph.D. Thesis, Sunchon National Univ., Korea (in Korean).
  10. Kantayos, V., and C.-H. Bae. 2019. Optimization of shoot induction, histological study and genetic stability of in vitro cultured Pisum sativum cv. 'Sparkle'. Korean J. Plant Res. 32:19-28. https://doi.org/10.7732/kjpr.2019.32.1.019
  11. Kim, D-g., V. Kantayos, D.-K. Kim, H.-G. Park, H.-H. Kim, E.-S. Rha, S.-C. Lee and C.-H. Bae. 2016. Plant regeneration by in vitro tissue culture in Korean soybean (Glycine max L.). Korean J. Plant Res. 29:143-153 (in Korean). https://doi.org/10.7732/kjpr.2016.29.1.143
  12. Ma, X.-H. and T.-L. Wu. 2008. Rapid and efficient regeneration in soybean [(Glycine max (L.)] Merrill from whole cotyledonary node explants. Acta Physiol Plant 30:209-216. https://doi.org/10.1007/s11738-007-0109-3
  13. Mariashibu, T.S., V.R. Anbazhagan, S-Y. Jiang, A. Ganapathi and S. Ramachandran. 2013. A comprehensive survey of international soybean research-genetics, physiology, agronomy and nitrogen relationships. Chapter-20: In vitro regeneration and genetic transformation of soybean: Current Status and Future Prospects. Intec Open, http://dx.doi.org/10.5772/54268.
  14. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Raveendar, S., A. Premkumar, S. Sasikumar, S. Ignacimuthu and P. Agastian. 2009. Development of a rapid, highly efficient system of organogenesis in cowpea Vigna unguiculata (L.) Walp. S. Afr. J. Bot. 75:17-21. https://doi.org/10.1016/j.sajb.2008.05.009
  16. Raza, G., M.B. Singh and P.L. Bhalla. 2017. In vitro plant regeneration from commercial cultivars of soybean. Bio. Med. Res. Int. 2017, Article ID 7379693, 1-9.
  17. Sairam, R.V., G. Franklin, R. Hassel, B. Smith, K. Meeker, N. Kashikar, M. Parani, D. Al. Abed, S. Ismail, K. Berruy and S.L. Goldman. 2003. A study on the effect of genotypes, plant growth ragulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell Tiss. Org. Cult. 75:79-85. https://doi.org/10.1023/A:1024649122748
  18. Thomas, T.D. 2007. Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn, an endangered medicinal plant. Acta Physiol Plant 29:455-461. https://doi.org/10.1007/s11738-007-0055-0
  19. Tie, M., Q. Luo, Y. Zhu and H. Li. 2013. Effect of 6-BA on the plant regeneration via organogenesis from cotyledonary node of cowpea (Vigna unguiculata L. Walp). J. Agric. Sci. 5(5), doi: 10.5539/jas.v5n5p1
  20. Wang, H., M. Li, Y. Yang, J. Dong and W. Jin. 2015. Histological and endogenous plant growth regulators changes associated with adventitious shoot regeneration from in vitro leaf explants of strawberry (Fragaria ${\times}$ ananassa cv. 'Honeoye'). Plant Cell Tiss. Org. Cult. 23(3):479-488.
  21. Yan, B., M.S. Srinivasa-Reddy, G.B. Collins and R.D. Dinkins. 2000. Agrobacterium tumefaciens-mediated transformation of soybean [Glycine max (L.) Merrill.] using immature zygotic cotyledon explants. Plant Cell Rep. 19(11):1090-1097. https://doi.org/10.1007/s002990000236
  22. Yildiz, M., S. Ozcan and C. ER. 2002. The effect of different explant sources on adventitious shoot regeneration in flax (Linum usitatissimum L.). Turk J. Biol. 26:37-40.
  23. Yue-Sheng, Y., K. Wada and Y. Futsuhara. 1990. Comparative studies of organogenesis and plant regeneration in various soybean explants. Plant Sci. 72(1):101-108. https://doi.org/10.1016/0168-9452(90)90191-P
  24. Zhang, F., C. Chen, H. Ge, J. Liu, Y. Luo, K. Liu, L. Chen, K. Xu, Y. Zhang, G. Tan and C. Li. 2014. Efficient soybean regeneration and Agrobacterium-mediated transformation using a whole cotyledonary node as an explant. Biotechnol. Appl. Biochem. 61(5):620-625. https://doi.org/10.1002/bab.1207

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