• 한국자원식물학회지
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• 제19권2호
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• pp.265-272
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• 2006

본 연구는 포자체 배양을 통하여 참쇠고비의 효율적인 번식방법을 구하고자 수행되었다. 배양절편의 기원(근경, 엽병, 엽신)과 절편의 균질화가 신초 계생에 미치는 영향을 조사한 결과, 오직 근경 조직에서만 기관발생능력이 관찰되었고, 식물체 재생은 배양재료를 균질화함으로써 더욱 촉진되었다. MS배지의 무기물 농도를 2배로 첨가한 배지에서 신초의 증식과 생장이 활발히 이루어졌다. 신초 재생에 적합한 배지의 질소함량은 MS배지의 1/2배 수준 이었고, 적정 sucrose농도는 1%였다. 또한 배지에 $NaH_2PO_4$를 첨가함으로써 재생된 식물체의 생육이 전반적으로 촉진되었다. 근경절편의 기관형성능력은 kinetin과 IBA를 혼용 처리함으로써 촉진되었으며, $0.1\sim0.2%$의 활성탄과 생장조절물질을 혼용 처리한 결과, 다수의 싹이 뭉친 듯이 보이는 primordia 형태의 발달이 억제되었고, 포자체의 정상적인 형태형성이 향상되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.15-15
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• 2019

The walnut (Juglans regia L.), a member of the Juglandaceae, is native to the mountain ranges of central Asia. This species of walnut is valued commercially for its nuts and in some areas for its timber. The seeds of walnut are recalcitrant and it has strong integument dormancy and their germination is irregular, making its natural propagation difficult. Low percentage of seed germination and long propagation cycle are the main problems of propagation. This study was conducted medium composition on in vitro plantlet regeneration from axillary buds of walnut. It has proved to be the most generally applicable and reliable method of in vitro propagation. Micropropagation culture that axillary buds are excised aseptically enables faster multiplication of plants. The axillary buds of walnut new cultivar "Sinlyeong" were cultured on two basal media which contained the different plant growth regulators depending on the respective shooting and rooting stage. After 12 weeks, the shoot generation rate was 85.3%, the shoot number and its length were 1.9/explant and 2.7 cm in the most favorable medium composition. The percentage of rooting was 25.4%. From these results, it was found the optimum basal medium and plant growth regulator for in vitro plant regeneration from axillary buds of walnut new cultivar "Sinlyeong". However, we have continued to search the other medium additives to enhance the rate of walnut root.

• 생물환경조절학회지
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• 제16권4호
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• pp.309-313
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• 2007

본 연구는 배지의 종류 및 부피가 절화 장미의 발근과 발근 후 생육에 미치는 영향을 알아보기 위하여 수행하였다. 실험은 유리온실의 철재 베드에 높이 1.5m의 아치형 터널을 설치하고 습도유지를 위하여 fog(200sec./5min. or 100sec./5min) 가습하고, 삽목초기에 차광률 55%의 한랭사로 차광하였다. 실험재료는 Rosa hybrida 'Red Sandra'와 'Little Marble' 두 품종을 사용하였다. 배지의 종류에 따른 발근 차이를 알아보기 위하여 10가지 배지(토실이 상토, 암면큐브, phenolic foam, perlite, vermiculite, 또는 perlite와 peatmoss 1:1, perlite와 coir 1:1, perlite와 rockwool 1:1, rockwool 1:1, rockwool과 peatmoss 1:1, rockwool과 coir 1:1, v/v)를 준비하여 삽목하였다. 발근배지의 부피에 따른 발근 및 생육의 차이를 알아보기 위하여 3가지의 배지(토실이 상토, 암면큐브, phenolic foam)를 각각 3가지 부피$(3{\times}3{\times}3cm,\;5{\times}5{\times}5cm,\;7{\times}7{\times}7cm)$에 삽목하였다. 배지의 종류를 달리한 실험은 두 품종 모두에서 Rockwool 1: Peatmoss 1 배지에서 발근과 생육에서 가장 좋은 결과가 나타났으며, 배지의 부피에 따른 발근율과 모든 생육은 배지의 용적이 커질수록 좋았다.

• 한국자원식물학회지
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• 제27권1호
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• pp.43-50
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• 2014

본 연구는 '녹색 꽃잎 도라지'의 기내배양 시 배지구성물질의 적정농도 구명에 의한 대량번식을 목적으로 실시하였다. '녹색 꽃잎 도라지'의 절을 배양재료로 배양조건은 MS배지의 여러가지 구성물질의 농도를 달리한 결과, 1/4MS 배지에서 가장 양호한 부정근의 형성을 보였으나 생장은 1/2MS배지에서 좋았다. Sucrose첨가는 농도가 높을수록 신초와 부정근의 형성 및 생장이 좋았다. 활성탄은 무첨가구에서 가장 많은 부정근의 형성과 양호한 생장을 보였다. 배지의 pH는 4.8로 조절된 배지에서 가장 많은 부정근을 형성하였으며, pH가 높아질수록 그 형성은 낮아지는 경향을 보였고, 부정근과 신초의 생장 또한 pH 4.8에서 가장 왕성하였다. Agar 농도별 실험에서 부정근의 형성과 생장은 그 농도가 낮아질수록 양호한 경향을 보여 가장 낮은 첨가구인 0.4% 농도구에서 가장 많은 부정근의 형성과 왕성한 생장을 보였다. 생장조절제를 혼용 첨가한 경우 신초의 형성은 BA와 IAA의 혼용구가 kinetin과 IAA 또는 NAA 혼용구에 비해 효과적이었으며, BA 0.1 mg/L와 IAA 0.5 mg/L 혼용구에서 절편체당 3.9개로 가장 많은 신초가 형성되었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.24-24
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• 2021

The Cassava (Manihot esculenta Crantz) is one of the major food crops in the tropical or subtropical regions. Recently, clean planting materials of improved cassava cultivars are in high demand. Problems in the propagation of cassava are virus vulnerable and low rates of seed germination. Thus, the study was undertaken to develop an efficient in vitro mass propagation protocol of Manihot esculenta Crantz. So we tried to optimize protocols for mass production from axillary buds of Cassava. Young and actively growing stem segments were excised from adult plants of cassava. Samples were cut into a 3~4 cm nodal segments with axillary buds, and cultivated in the different medium supplemented with various plant growth regulators for 4 weeks. For shoot multiplication, axillary buds approximately 1 cm in length were taken from in vitro derived shoots and subcultured. After 4~6 weeks, the shoot generation rate showed 55.6%. The shoot number and its length was 1.0/explant and 2.3 cm in the most favorable medium composition. The auxin β-indolebutyric acid(IBA) 0~2.0 mg/L was proved to be effective on root development. Plantlets with fibrous roots easily generated tuberous roots in vitro. The tuberous roots were induced only when both kinetin and IBA were used in combination. after 8 weeks, the root generation rate showed 100%. The root number and its length was 17.2/explant and 2.2 cm in the most promising medium composition. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1622-1628
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• 2014

DagA, a ${\beta}$-agarase, was produced by cultivating a recombinant Streptomyces lividans in a glucose medium or a mixed-sugar medium simulating microalgae hydrolysate. The optimum composition of the glucose medium was identified as 25 g/l glucose, 10 g/l yeast extract, and $5g/l\;MgCl_2{\cdot}6H_2O$. With this, a DagA activity of 7.26 U/ml could be obtained. When a mixed-sugar medium containing 25 g/l of sugars was used, a DagA activity of 4.81 U/ml was obtained with very low substrate utilization efficiency owing to the catabolic repression of glucose against the other sugars. When glucose and galactose were removed from the medium, an unexpectedly high DagA activity of about 8.7 U/ml was obtained, even though a smaller amount of sugars was used. It is recommended for better substrate utilization and process economics that glucose and galactose be eliminated from the medium, by being consumed by some other useful applications, before the production of DagA.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.372-375
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• 2000

Lactobacillus cristatus KLB 46 isolated from Korean woman was grown on supplemented whey medium and medium compositions were optimized for maximum viable cell count. Among the nitrogen sources tested, beef extract yielded the highest viable cell number. When corn steep liquor was applied as an additional nitrogen source, the viable cell number was highest $(3.11{\times}10^9\;CFU/ml)$ in the medium containing 50g/ l corn steep liquor and 10g/ l beef extract. The highest viable cell $count(5.00{\times}10^9\;CFU/ml)$ was obtained from the supplemented whey medium that contains beef extract(10g/ l ), corn steep liquor(50g/ l ), tween 80(0.1%, v/v) and trace amounts of sodium acetate(5g/ l ), dipotassium phosphate(2g/ l ), magnesium sulfate(0.1g/ l ), and manganese sulfate (0.05g/ l ).

• 한국작물학회지
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• 제45권1호
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• pp.32-37
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• 2000

The in vitro culture of rice panicles is a culturing technique only panicle without other organs in culture solution containing organic substance, so that would be useful to study how assimilate supply affects grain development and maturation. To find the optimum stage for in vitro culture, rice panicles grown in greenhouse were sampled periodically after anthesis and cultured in nutrient medium. The panicles older than 1 weeks after anthesis had produced normal grains. Grain-filling was apparently dependent upon sucrose concentration (8-12 %) in medium, but not affected by nitrogen concentration supplied with glutamine. As far as rice panicle was supplied with sucrose and N in nutrient medium, grains continued accumulation of dry matter and maturation regardless to light condition. Considerably, grain-filling was improved when panicles were positioned horizontally inside flask, so that each grain was partially submerged to nutrient medium.

• 한국실내디자인학회논문집
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• 제21권6호
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• pp.186-193
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• 2012

This study is to reveal the periodical form of the 4 staged building sites with the size and the site composition of the master plan and floor plan of the Ungjindan (altar) from the Ungjindan excavation works in 2011. In order to project the research results aiming to the purpose of the study, the basic study was done with collecting data about shrine architecture for its architectural characteristics and case studies with ancestral facilities such as the Ak hae dok (national-level ancestral ritual to the big mountain, ocean and river) to understand the exact form of the site plan and architectural composition elements through the general information and excavation status. In addition, with the current situation and information from the excavation works the planned measurement scale will be calculated in inference for the size of the construction by stages and speculate the floor plan composition of the shrine architecture.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1997년도 춘계학술발표회논문집(2)
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• pp.341-344
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• 1997

A vitrification process is under development in KEPRI for the treatment of low-and medium-level radioactive waste. Although the project is for developing and building Vitrification Pilot Plant in Korea, one of KEPRI's concerns is the quality control of the vitrified glass. This paper discusses a methodology for the estimation of glass composition by on-line measurement of molten glass properties, which could be applied to the plant for real-time quality control of the glass product. By remotely measuring viscosity and density of the molten glass, the glass characteristics such as composition can be estimated and eventually controlled. For this purpose, using the database of glass composition vs. physical properties in isothermal three-component system of SiO$_2$-Na$_2$O-B$_2$O$_3$, a software TERNARY has been developed which determines the glass composition by using two known physical properties(e.g. density and viscosity).