• Korean Journal of Medicinal Crop Science
• /
• v.10 no.3
• /
• pp.162-166
• /
• 2002

(Z)-ocimene+limonene, $(E)-{\beta}-ocimene$ and citronellal showed seasonal variation in the leaves of Z. schinifolium. Especially estragole was detected at fruiting stage regardless of collection sites. Common variation components in the leaves of Z. piperitum at all collection sites were (Z)-3-hexenol, ${\alpha}-pinene$, limonene and citronellal. The compositions with monthly variation in Z. schinifolium at arboretum were ${\alpha}-pinene$, myrcene, (Z)-3-hexenyl acetate, ${\alpha}-phellandrene$, (Z)-ocimene+limonene, ${\beta}-phellandrene$, linalool, geranyl acetate while in Z. piperitum were hexanal, (Z)-3-hexenol, (E)-2-hexenal, hexanol, ${\alpha}-pinene$, (Z)-ocimene, limonene, citronellal, geranyl acetate, ${\beta}-caryophyllene$. Estragole was not detected in Z. schinifolium leaves at arboretum due to too young tree to bearing fruit on it.

• Korean Journal of Medicinal Crop Science
• /
• v.13 no.4
• /
• pp.133-137
• /
• 2005

Experiments were conducted to find the optimal acclimatization conditions for Eleutherococcus senticosus plantlets regenerated from bioreactor cultured somatic embryos, various acclimatizing conditions were compared regarding both survival rate and growth of the plantlets. Among the various temperature and artificial soil tested, the highest survival rate (88%) was observed when plantlets were acclimatized in Klasmann bed soil at $10^{\circ}C$ When in vitro plantlets directly transplanted to field environment, shading treatment was necessary and 50% shading was more effective than 30% shading. Transplanting season were also important for successful acclimatization of in vitro cultured plantlets, transplanted on March 20 with 50% shading exhibited the best survival rate and further growth.

• Korean Journal of Medicinal Crop Science
• /
• v.15 no.2
• /
• pp.73-81
• /
• 2007

An efficient regeneration system was developed using leaf, petiole, and internode explants. Highly embryogenic callus was obtained following cultivation on MS basal nutrient supplemented with 2 $mg/{\ell}$ 2,4-D. Globular, heart, torpedo and cotyledon shaped somatic embryo were produced from the surface of embryogenic callus. Direct shoot regeneration without intermediate callus formation has been achieved on MS medium supplemented NAA and BAP. The percentage of response varies with different concentration of auxin and cytokinin treated individually or in combination. The best shoot regeneration response (54.28%) and number of shoot per explant (12.67) were achieved on the medium supplemented with 0.1 $mg/{\ell}$ NAA and 1 $mg/{\ell}$ BAP. The regenerated shoot transformed into young plant when cultured into elongation and root induction medium. More than 90% of in vitro propagated plants could survive when transferred to the greenhouse for acclimation. This optimized regeneration system can be used for rapid shoot proliferation and genetic transformation.

• Korean Journal of Medicinal Crop Science
• /
• v.20 no.5
• /
• pp.339-344
• /
• 2012

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

• Journal of Korean Medical classics
• /
• v.31 no.3
• /
• pp.1-32
• /
• 2018

Objectives : This paper seeks to discover and compile the information of the types of Korean medical herbs that are recorded in China's medial herbs literatures. Following this, the special characteristics of each herb and the differences in the description of each Korean herb found in different literatures and ages. Methods : First, the literatures were searched for the names of countries, regions, and people used in Korean peninsula, and a database was compiled based on the findings. Texts with no relation to the subject matter were discarded, and the rests were listed in the order of publication. The special characteristics of the medical herbs found in these literatures were then studied. Results & Conclusions : Total of 69 medical herbs were found in 34 literatures. These medical herbs could be categorized into three: those with superior quality, those that are different from the medical herbs used in China, and those that are not available in China. The medical herbs with superior quality are: Gonpo(昆布), Insam(人參), Omija(五味子), Tosaja(?絲子), Haesongja(海松子), Sesin(細辛), Baekbuja(白附子) and more. We can deduce that these medical herbs were regarded as superior in quality compared to their counterparts in China because they grow in the northeast region where pine tree thrive and a home to many areas with seas and mountains, and also with four distinctive seasons.

• Korean Journal of Medicinal Crop Science
• /
• v.19 no.1
• /
• pp.24-30
• /
• 2011

This text was analyzed and investigated the vegetation and floristic composition by cluster analysis and classification of phytosociological method, to evaluate the species composition, habitat environment and community structure of Liriope platyphylla and Liriope spicata group in Korea. The southeast slope gradient of the habitat of L. platyphylla and L. spicata was 6.7 to 8.4%, and the habitat altitude of L. platyphylla (41.0 m), L. spicata (114.9 m) was different. Habitat distribution of L. spicata was broader than L. platyphylla. Appearing plants of L. platyphylla and L. spicata group was 58 taxa, 99 taxa, respectively, and Coverage of tree layer was 87.5%, 92.5% respectively. In genus Liriope group, the highest appearing frequency of plant grow in the moist valley as Quercus serrata. Thus, plants of genus Liriope growth was better in moist shade. The vegetation of L. platyphylla group was classified into Quercus serrata community, Castanopsis sieboldii community, Pinus densiflora community and Pinus thunbergii community, and the Liriope spicata group was classified into Quercus serrata community, Quercus alien community, Quercus acutissima community, Prunus verecunda community, Robinia pseudoacacia community, Pinus densiflora community and Pinus thunbergii community. In genus Liriope group, Quercus serrata and Pinus densiflora communities was the closest the similarities.

• Korean Journal of Medicinal Crop Science
• /
• v.10 no.2
• /
• pp.126-131
• /
• 2002

We investigated the high yield capacity of Hwangchil lacquer for selection and identification of major aromatic components of the selected trees as well. The Hwangchil lacquer showed the difference yields by different habitat, tree ages and individual character. The selected trees showed high yield capacity of Hwangchil lacquer. We also investigated the essential oil contents and its the main components to analysis the potent of mass propagation. The major compounds were 1,6-atadiene-3-ol., ${\alpha}-terpinene,\;{\alpha}-cubebene,\;{\alpha}-ylangene,\;{\alpha}-copane,\;{\beta}-elemene,\;germacrene-D,\;{\beta}-selinene,\;{\alpha}-selinene,\;{\delta}-cadinene,{\gamma}-cardinene$, germacrene B, germacrene D-4-ol. The most principle component was germacrene D among them respectively. However, the difference of relative content ratio of each major compound was showed by individuals, population and native areas in the selected trees.

• Korean Journal of Medicinal Crop Science
• /
• v.11 no.2
• /
• pp.122-126
• /
• 2003

This study was conducted to investigate plant growth characteristics and anatomical studies of Korean mistel (Viscum album var. coloratum (Kom.) Ohwi). Parasite position was upper part that has a plenty of sunshine area 5 to 10m apart from the ground, regardless host tree. Stem developed two branch stems from the each basal stem, leaf was grown from the end stems as opposite. Fruit has a light yellow color and berry size was 7 to 8 mm long diameter and it contains seed. Floral bud differentiation initiated with forming rounded dome and arch-shaped floral apex at the beginning stage, and produced heart-shaped sepal following the differentiation of leaf primordia. Floral bud formation processed centeral part first and following bilateral sides were developed in order. Leaf was thick and stoma and guard cell were observed from the two or three inner layers from the epidermis. Mistletoe penetrated cork cambium of host plant and was growing by rooting on xylem. Korean mistel connected to host plant through parasitic organ, haustorium structurally and physiologically.

• Korean Journal of Medicinal Crop Science
• /
• v.22 no.1
• /
• pp.8-16
• /
• 2014

The present study was carried out to evaluate the antioxidant and anti-inflammatory activities of three extracts (hot water, 50% ethanol and mixed solvent;water, ethanol, butylene glycol, propylene glycol) of dried chestnut, chestnut shell, chestnut leaves and dried chestnut leaves obtained from Castanea crenata tree. When conducted DPPH assay, radical scavenging activity of ethanol extract of chestnut shell was the highest with $IC_{50}$ $10.8{\mu}g/mL$ among four extracts from these parts (p < 0.05). In additional results by the xanthine oxidase assay, antioxidant activity showed that water extract of chestnut leaves showed the highest xanthine oxidase inhibitory activity in the tested extracts (p < 0.05). Futhermore, extracts of chestnut shell and leaves exhibited no cytotoxicity in RAW 264.7 cells (p < 0.05). Also, anti-inflammatory activity by NO assay showed LPS-induced NO was significantly inhibited following treatment with extracts of chestnut shell and leaves of 3mg/mL (p < 0.05). These data suggest that extract of chestnut shell have antioxidant and anti-inflamantory activity including chestnut leaves. Therefore, it is considered that Castanea crenata research range and selection of functional material can broaden chestnut shell to other fractions such as chestnut and chestnut leaves.

• Korean Journal of Medicinal Crop Science
• /
• v.6 no.3
• /
• pp.204-209
• /
• 1998

Antiinflammatory and antioxidative effects of mulberry fruit were investigated by using bioassay screening system. The extract of muberry fruits showed inhibitory effect in phospholipase $A_2$ activity and cyclooxygenase II activity. Among nine varieties of mulberry tree, the antiinflammatory activities of the Shingwangppong, Ficus, Cheongilppong and Keomseolppong were higher than that of the other varieties. Also, antioxidative activity of mulberry fruit was examined by DPPH free radical scavenging method. The radical scavenging activity of the mulberry fruit decreased as following order : Shingwangppong > Keomseolppong > tetraploid Ficus> diploid Ficus.