• The Korea Journal of Herbology
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• v.27 no.6
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• pp.1-6
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• 2012

Objectives : ROS are involved in a wide spectrum of diseases including chronic inflammation and cancer. S.chinensis Baill, a perennial herb commonly called Chinese lizard's tail or Sam-baek-cho in Korea, is used for the treatment of edema and inflammatory diseases in the Oriental folk medicine. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the two extracts, water(WE) and ethyl acetate(EAE) from S.chinensis Baill. Methods : Anti-oxidant activity was evaluated using Fe2+ chelating and hydroxyl radical scavenging assay. DNA cleavage assay, and western blot and immunostaining for phospho-p65 were performed to evaluate anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell. Results : In Fe2+ chelating activity and hydroxyl radical scavenging activity, WE showed more strong scavenging activity for hydroxyl radical than EAE. WE scavenged hydroxyl radical by 12% at 3.2 ${\mu}g/ml$, 21% at 16 ${\mu}g/ml$, 32% at 80 ${\mu}g/ml$, 66% at 400 ${\mu}g/ml$ and 82% at 2000 ${\mu}g/ml$, respectively. In addition, WE showed more strong chelating activity than EAE. WE chelated Fe2+ ion by 1.1% at 3.2 ${\mu}g/ml$, 8.2% at 16 ${\mu}g/ml$, 26.3% at 80 ${\mu}g/ml$, 72% at 400 ${\mu}g/ml$ and 89% at 2000 ${\mu}g/ml$, respectively. Also, WE inhibited oxidative damage via its anti-oxidant activity. In anti-inflammatory effect, EAE inhibited NO production and iNOS expression. In addition EAE suppressed the NF-${\kappa}B$ and MAPK signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, these data indicate that S. chinensis Baill, shows anti-oxidant activity and anti-inflammatory effect.

• Biomedical Science Letters
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• v.17 no.1
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• pp.69-77
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• 2011

In this study, we evaluated the protective effects of supercritical extracts and two step ethanol extracts after supercritical extraction from Schizandra chinensis on antioxidant activities and oxidative DNA and cell damages. Supercritical extracts removed DPPH (1,1-diphenyl-2-picryldrazyl) radical by 85.5% at 200 ${\mu}g$/ml, but showed low activities of scavenging and chelating the hydroxyl radical and ferrous iron. However, two step ethanol extracts showed low activities of scavenging the DPPH radical, but removed the hydroxyl radical by 86% at 200 ${\mu}g$/ml. In addition, we tested the activities of extracts for reducing hydroxyl radical-induced DNA and cell damage. Two step ethanol extracts showed protective effect against the oxidative DNA damage by reducing DNA segmentation, inhibiting DNA migration and decreasing the expression of phospho-H2AX. Also, two step ethanol extracts showed protective effect against the oxidative cell damage by inhibiting lipid peroxidation and increasing the expression of p21 protein. Taken together, we suggest that two step ethanol extracts from S. chinensis have a role as useful inhibitors against oxidative damages.

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.304-305
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• 2011

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.362-363
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• 2011

• Korean Journal of Plant Resources
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• v.33 no.5
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• pp.460-466
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• 2020

In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of leaves from Rodgersia podophylla against human colorectal cancer cells, HCT116. RPL dose-dependently decreased the cell viability through RPL-induced apoptosis in HCT116 cells. RPL induced inactivation the nuclear factor κB(NF-κB) through blocking IκB-α degradtion and P65 nuclear accumulation. The inhibition of GSK3β by LiCl attenuated RP-L-mediated NF-κB signaling inactivation. In addition, RP-L induced GSK3β activation. Based on these findings, RPL may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.

• Korean Journal of Plant Resources
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• v.36 no.4
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• pp.257-263
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• 2023

Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, water extracts from Rosa acicularis leaves (RAL) increased the production of immunostimulatory mediators and phagocytic activity in RAW264.7 cells. RAL increased p62/SQSTM1 expression. Inhibition of TLR4, JNK, and PI3K/AKT blocked RAL-mediated increase of p62/SQSTM1. RAL activated JNK and PI3K/AKT signaling. RAL-mediated activations of JNK and PI3K/AKT signaling were reversed by TLR4 inhibition. Taken together, it is believed that RAL-mediated autophagy may be dependent on activating via TLR4-dependent activation of JNK and PI3K/AKT signaling in macrophages.

• Preventive Nutrition and Food Science
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• v.3 no.4
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• pp.324-328
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• 1998

We screened the methanol extracts from 133 plant species growing in Korea for antimicrobial activity against enterohemorrhagic Escherichia coli 0157 : H7. Those are selected from three plant grouping ; traditional medicinal herbs, edible plants, and flowers. They were tested by disk diffusion assay. From evaluation of the inhibition zone diameter of microbial growth, we found that the flower extract of Rhododendron Schilpenbachii Max had the most significant antimicrobial activity against this bacteria. Extracts from most of the vegetables and plants did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and seeds of Prunus Dallicina L. did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and the seeds of Prunus sallicina L.

• Advances in Traditional Medicine
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• v.9 no.2
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• pp.182-185
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• 2009

Amoora cucullata (Meliaceae), a mangrove plant, has folkloric reputation as a medicinal agent in Bangladesh. In this study, the n-hexane, ethyl acetate and methanolic extracts of the stem bark of this plant were subjected to microbiological investigation and brine shrimp lethality bioassay. In case of antimicrobial screening, the ethyl acetate and methanolic extracts appeared to be potent in terms of both zone of inhibition and spectrum of activity showing the average zones of inhibition 8 - 14 mm and 9 - 16 mm, respectively. In the brine shrimp lethality bioassay, the methanolic extract demonstrated highest cytotoxicity having $LC_{50}$ of $0.549{\mu}g/ml$, whereas the ethyl acetate and n-hexane extract showed $LC_{50}$ of 7.943 and $17.180{\mu}g/ml$, respectively.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.82-82
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• 2018

본 연구는 국내에 자생하는 자원식물들의 살초활성을 조사하여 식물생장억제물질을 활용한 환경친화형 제초제 개발에 요구되는 기초 자료를 확보하기 위해 수행되었다. 국내 자생 식물 101종을 식물체 부위별로 나누어 MeOH로 추출하여 획득된 시료 129점에 대해 돌피(Echinocholoa crus-galli P.B. var. formosensis Ohwi)를 이용한 살초활성 검정을 실시하였다. 시료를 소량의 methanol로 녹여 직경 5.5cm petri dish 상의 Whatman No. 2 여지에 균일하게 흡착시킨 후, fume hood 내에서 용매를 제거하고 1mL의 증류수를 첨가하였다. 돌피 종자를 15립씩 3반복으로 파종하여 $28^{\circ}C$, 5,000lux의 growth chamber에 치상하고, 7일 후 시험구 당 균일하게 자란 10개의 유묘를 선발해 초장 및 근장을 조사하였으며, 살초활성 평가를 위해 대조구 개체의 초장과 근장에 대한 생장억제율을 산출하였다. $1,000{\mu}g/mL$ 농도에서 101종 129점 MeOH 추출물들의 돌피 유묘 뿌리 생장에 대한 억제율을 조사한 결과, 80% 이상을 나타낸 식물 시료는 가죽나무 줄기, 누린내풀 지상부, 단풍취 뿌리, 두릅나무 지상부, 백양꽃 전초, 백양꽃 지하부, 병조희풀 경엽부, 산사나무 잎 가지, 삽주 뿌리, 상사화 전초, 상사화 지하부, 석산 뿌리, 애기송이풀 전초 등 17점이었다. 이들 중 90% 이상 고활성을 나타낸 단풍취 뿌리, 두릅나무 지상부, 백양꽃 전초, 상사화 지하부, 석산 뿌리 유래의 추출물에는 새로운 환경친화형 제초제 개발소재로써 활용할 수 있는 살초활성물질들이 함유되어 있는 것으로 생각된다.

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.373-374
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• 2010