• 대한화장품학회지
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• 제33권2호
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• pp.127-135
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• 2007

본 연구는 한방화장품의 원료로써 사용이 되는 약용식물들의 부위별 및 추출용매에 따른 추출물의 효능의 차이를 기술하였다. 기존의 단일성분 분석법보다 실제로 성분의 복합체인 추출물에 대하여 항산화와 UV흡수 관련 효능효과 시험법을 적용하였다. 약효를 가지는 약용식물들을 추출할 때 고려해야 할 조건이 있다. 같은 약용식물이라도 채취시기, 채취장소, 채취부위와 같은 채취조건들과 용매의 종류, 추출시간, 추출온도와 같은 추출조건들을 고려하여야 한다. 조건 중에서 추출용매와 채집부위는 효과에 있어서 매우 중요한 요인이다. 약용식물에 대한 관심이 증가하면서 여러 가지의 채취조건과 추출조건을 연구하여 한방원료의 개발에 있어 좀더 다양한 적용이 요청되고 있다. 본 연구에서는 약용과 식용으로 널리 사용되는 식물(Terminalia chebula, Syzygium aromaticum, Paeonia lactiflora, Morus alba, Scutellaria baicalensis)을 선정하여 추출용매와 채집부위를 변경시킴으로써 용매와 부위에 따른 효능의 차이를 확인하였다. 이 결과로 추출시 적당한 조건을 선택한다면 사용되는 약용식물의 양을 줄이거나 좀더 효과적인 결과를 얻을 수 있다.

• Archives of Pharmacal Research
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• 제14권1호
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• pp.81-86
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• 1991

Dry powder and different extracts of Agave lophantha were tested against Biomphalaria alexandrina. The results showed that the butanol extract has high molluscicidal activity. The activity of the dry powder has been found to be stable under the effect of some simulated field conditions. Also the toxicological effect of the plant on mice was tested through determination of certain parameters such as total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and acid phosphatase enzymes as well as histopathological study on liver and kidney.

• 한국약용작물학회지
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• 제17권1호
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• pp.21-25
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• 2009

The results on the useful functional compounds of kenaf (Hibiscus cannabinus L.) leaves cultivated in reclaimed lands and the biological activity effects of extracts were as follows. On 98 days after sowing (DAS) Tainung-2 showed the highest total chlorophyll content (1.68 mg/g), and on 141 DAS Dowling showed higher chlorophyll content (1.50 mg/g) than the other two did. In all cultivars the total chlorophyll content was higher on 141 DAS than on 98 DAS. Total polyphenol and total flavonoid contents were the highest in Tainung-2 (30.50 mg/g and 57.03 mg/g, respectively), and total polyphenol and total flavonoid contents (30.50 mg/g and 57.03 mg/g, respectively) were the highest in 30% ethanol extraction. Ascorbic acid contents were higher on 141 DAS than on 98 DAS in three cultivars. SOD activities of kenaf leaf extract were generally over 90%. DPPH radical scavenging activity of Tainung-2 was higher than others.

• 한국약용작물학회지
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• 제17권3호
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• pp.173-178
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• 2009

A variety of herbs and plants have been traditionally used in oriental folk medicine for the treatment of inflammatory diseases. In our attempt to search for anti-inflammatory agents from natural products, we investigated 64 methanol extracts from 42 medicinal plants belonging to 10 families which were evaluated for inhibitory activities of NO production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Among them, 16 extracts exhibited inhibitory activities of NO production ($IC_{50}$ values ranging from 59.6 to 94.7 ${\mu}g/m{\ell}$). Only the extract from aerial parts of Hosta lancifolia (H. lancifolia) did not exert cytotoxic effects at the concentrations tested. The extract from H. lancifolia decreased the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in activated macrophage RAW 264.7 cells in dose-dependent manner. The results suggest that the extract may contain bioactive compounds that suppress expression of pro-inflammatory cytokines, which may prove beneficial with regard to the development of natural agents for prevention and treatment of inflammatory diseases.

• 생명과학회지
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• 제13권5호
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• pp.604-617
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• 2003

안전성이 확보된 식용, 또는 약용식물로부터 항캔디다 활성이 우수한 항진균 후보물질을 개발하기 위해, 298종의 천연물로부터 추출부위 및 추출 용매를 달리하여 384종의 추출물을 제조하였다. 제조된 추출물의 항캔디다 활성을 disc-paper를 이용한 생육 저지환의 유무 및 크기 판정과 microbroth dilution법을 이용하여 C. albicans KCTC 1940과 S. cerevisiae IFO 0233를 대상으로 평가하였다. 그 결과 20종 식물로부터 조제된 25종의 추출물이 우수한 항캔디다 활성을 나타냄을 확인하였고, 그 중에서도 멍석딸기, 정향, 천황련, 작약, 모란, 대극잎 추출물은 강력 한 항캔디다 활성$(MIC=50 \mug/m\ell)$을 보였다. 특히, 멍석딸기(에틸아세테이트 추출물), 정향(부탄올 추출물), 호장근(에틸아세테이트 추출물), 작약(부탄올 추출물), 대극잎(메탄올 추출물)의 경우에는 C. albicans에 높은 선택독성을 나타내어, 안정성이 확보된 식물 추출물로부터 새로운 진균 감염증 치료제 개발이 가능함을 제시하였다.

• Natural Product Sciences
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• 제28권3호
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• pp.121-129
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• 2022

Despite considerable efforts, cancer remains an aggressive killer worldwide. Chemotherapeutic drugs that are currently in use lead to destructive side effects and have not succeeded in fulfilling expectations. For centuries, medicinal plants are used for treating various diseases and are also known to have anticancer activity. The main aim of this research was to evaluate antiproliferative activity of saffron, clove, fenugreek, and green tea on Vero and MDA-MB-231 cell lines and to subsequently analyze the effect of these extracts on IRAK-4, TAK1, IKK-alpha, IKK-beta, NF-Kappa B, IRF3, IRF7 genes in Toll Like Receptors (TLRs) pathway. Antiproliferative assay was done by Neutral Red Dye uptake assay. Methanolic extract of green tea was found to be most effective against both cell lines as IC₅₀ was achieved at least concentration of the extract. For molecular studies, MDAMB-231 cells were sensitized with methanolic extract of green tea at same IC₅₀, and RT-PCR was performed to determine the relative expression of genes. Expression of IRAK-4, TAK1, IKK-beta, NF-Kappa B, IRF3 genes was down regulated and IRF7 and IKKalpha was upregulated. Green tea has a potential cytotoxic effect on both cell lines which was demonstrated by its effect on the expression of (TLRs) pathway genes.

• Preventive Nutrition and Food Science
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• 제10권1호
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• pp.81-87
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• 2005

The objective of this study was to determine the radical scavenging activity, total phenolic content, antimicrobial activity, minimum inhibitory concentration (MIC) of ethanol extracts of 32 medical plant species that have been commonly used in medicinal plants. Total phenolic index of T. chebula exhibited the highest value (498.01㎎/g), followed by R. coreanus miquel (400.33㎎/g), Sanguisorba officinalis (368.25㎎/g), P. thumbergiana (259.74㎎/g) and Eugenia aromaticum (229.38㎎/g). Radical scavenging activity for the DPPH radical was highest in T. chebula (40.91%, p<0.01), followed by C. sappan (36.50%), S. officinalis (32.92%), R. coreanus miquel (26.54%) and P. thumbergiana (24.50%). The extracts from T. chebula, R. coreanus muquel, C. sappan, E. aromaticum, S. officinalis and C. japonica possessed outstanding antimicrobial activity against Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Lactobacillus plantarum. MIC was determined on those extracts that showed high efficacy against the test organisms. The most potent MIC values were seen for T. chebula extract against P. aeruginosa, S. aurusa, E. coli, B. subtilis, L. plantarum and S. Typhimurium at 7.8, 7.8, 15.6, 7.8, 125 and 31.2㎍/mL, respectivley. Furthermore, the total phenolic content and radical scavenging activity were very closely correlated for all samples (r=0.78). The coefficient correlations between total phenolic index and antimicrobial activity were 0.91 (E. coli), 0.91 (B. subtillis), 0.79 (P. aeruginosa), 0.79 (S. Typhimurium) and 0.70 (L. plantarum).

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.80-103
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• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth pormoting effect were measured using hair growth index As a result, Polygonum muitifiorum Thunb and Terrninalia chebula Retz. showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Morus alba L., Chaenomelis Fructus, Saussureae Radix, Angelicae Gigantis Radix, Polygonum multifiorum Thunb, and Angelica dahurica (Fischer) Bentham et Hooker f. showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of effects of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT-PCR analyses were performed. As a consequences, Mentha haplocalyx Briq., Cimicifuga foetida L., Eclipta prostrata (L.) L., Pinus densiflora S. et. Z, and Polygonum muitifiorum Thunb revealed the regulatory roles on the expression of growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another test for inhibition of microbial such as P. acne and P. ovale were also carried out to find whether these plant extracts have anti-microbial activities. Morus alba L. and Chaenomelis Fructus showed anti-microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• 셀메드
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• 제3권4호
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• pp.28.1-28.7
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• 2013

Careya arborea Roxb. (Lecythidaceae) is a significant medicinal plant known as kumbhi in Ayurveda. Though, most of the plant parts are used in traditional systems of medicine, bark and leaves constitute the important medicinal parts. The present review gives an account of the updated information on its phytochemical and pharmacological properties. The review reveals that numerous phytochemical constituents have been isolated from the plant possessing hepatoprotective, antioxidant, antimicrobial, anticoagulant, analgesic, antidiarrhoeal and various other important activities. Leaves are used in filaria, colic, loose motions and ulcers. Bark is used as an antipyretic, abortifacient, antipruritic and in smallpox, urinary discharges and rheumatic pain. Since last few decades, extensive exploration has been done to establish the biological activities and pharmacology of the extracts and plentiful chemical constituents including flavonoids, tannins, alkaloids, terpenoids and many other have been isolated.

• 한국자원식물학회지
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• 제10권3호
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• pp.213-220
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• 1997

The present study has been undertaken to characterize availability of citrus as a medicinal plant with antineoplastic property. The crude extracts from 40 species of fruits with 12 species of the local Citrus in Cheju island were evaluated on their potential activities against mouse P388 lymphocytic leukaemia in vitro. The percent cytotoxicity varied from 25.40 to 97.94% at a concentration of $100{\mu}g/mL$. Among 40 spp., 8 species showed high toxicity more than 90% against P388 cells and Cheongkyool(C. nippokoreana) exhibited the most cytotoxicity as 97.94%($IC_{50}=20.2{\mu}g/mL$). Nine varieties of C. junos were showed insiginicant cytotoxicity. In trifoliate orange, immature fruit was stronger than mature and peel extract showed higher cytotoxicity($IC_{50}=18{\mu}g/mL$) than the other tissues. Hexane fraction from methanol(MeOH) extract of trifoliate orange showed highly significant inhibition of cell growth($IC_{50}=3.9{\mu}g/mL$). In addition, its cytotoxicity increased remarkably from 3.95 to $0.40{\mu}g/mL$ as exposure time legthened. Cytotoxic activities of crude extracts were decreased considerably during a six months storage period. It was apparent that there is considerable variation in cytotoxicity, depending upon species, maturity and storage time of extracts. There was no meaningful cytotoxic difference between archicitrus and metacitrus in the genus Citrus.