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Inhibitory Effects of Methanolic Extracts of Medicinal Plants on Nitric Oxide Production in Activated Macrophage RAW 264.7 Cells  

Seo, Jin-Suk (Graduate School of Biotechnology, Kyung Hee University)
Lee, Tae-Hoon (Graduate School of Biotechnology, Kyung Hee University)
Lee, Sang-Min (Graduate School of Biotechnology, Kyung Hee University)
Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Seong, Nak-Sul (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Kim, Ji-Young (Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Korean Journal of Medicinal Crop Science / v.17, no.3, 2009 , pp. 173-178 More about this Journal
Abstract
A variety of herbs and plants have been traditionally used in oriental folk medicine for the treatment of inflammatory diseases. In our attempt to search for anti-inflammatory agents from natural products, we investigated 64 methanol extracts from 42 medicinal plants belonging to 10 families which were evaluated for inhibitory activities of NO production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Among them, 16 extracts exhibited inhibitory activities of NO production ($IC_{50}$ values ranging from 59.6 to 94.7 ${\mu}g/m{\ell}$). Only the extract from aerial parts of Hosta lancifolia (H. lancifolia) did not exert cytotoxic effects at the concentrations tested. The extract from H. lancifolia decreased the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in activated macrophage RAW 264.7 cells in dose-dependent manner. The results suggest that the extract may contain bioactive compounds that suppress expression of pro-inflammatory cytokines, which may prove beneficial with regard to the development of natural agents for prevention and treatment of inflammatory diseases.
Keywords
Plant Extracts; inducible Nitric Oxide Synthase (iNOS); Nitric Oxide (NO); Inflammation; Hosta lancifolia;
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Times Cited By KSCI : 3  (Citation Analysis)
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