Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Inhibitory Effects of Methanolic Extracts of Medicinal Plants on Nitric Oxide Production in Activated Macrophage RAW 264.7 Cells

약용식물 추출물에 의한 면역세포 산화질소 생성 억제 활성 분석

  • Seo, Jin-Suk (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Tae-Hoon (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Sang-Min (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Seong, Nak-Sul (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Ji-Young (Graduate School of Biotechnology, Kyung Hee University)
  • 서진숙 (경희대학교 생명공학원) ;
  • 이태훈 (경희대학교 생명공학원) ;
  • 이상민 (경희대학교 생명공학원) ;
  • 이승은 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 성낙술 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김지영 (경희대학교 생명공학원)
  • Published : 2009.06.30
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Abstract

A variety of herbs and plants have been traditionally used in oriental folk medicine for the treatment of inflammatory diseases. In our attempt to search for anti-inflammatory agents from natural products, we investigated 64 methanol extracts from 42 medicinal plants belonging to 10 families which were evaluated for inhibitory activities of NO production in lipopolysaccharide (LPS)-stimulated macrophage RAW 264.7 cells. Among them, 16 extracts exhibited inhibitory activities of NO production ($IC_{50}$ values ranging from 59.6 to 94.7 ${\mu}g/m{\ell}$). Only the extract from aerial parts of Hosta lancifolia (H. lancifolia) did not exert cytotoxic effects at the concentrations tested. The extract from H. lancifolia decreased the mRNA and protein levels of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in activated macrophage RAW 264.7 cells in dose-dependent manner. The results suggest that the extract may contain bioactive compounds that suppress expression of pro-inflammatory cytokines, which may prove beneficial with regard to the development of natural agents for prevention and treatment of inflammatory diseases.

Keywords

References

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