• Journal of Naturopathy
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• v.10 no.2
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• pp.86-92
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• 2021

Background: The presence of antioxidants was not confirmed in the medicinal plant Equisetum hyamale grass. Purposes: This study was to determine the antioxidant and elastase inhibition effects of extracts of E. hyemale. Methods: Antioxidant functions of E. hyemale stems and roots were measured and extracted with hot water (HW) and ethyl alcohol (70EOH, 100EOH). Results: The extraction yield of stems was higher in HW extraction than in ethyl alcohol extraction. The polyphenol content was significantly higher in the root extract than in the stem. Total flavonoid content of 70EOH extract was significantly higher in root extract than in stem. The elastase inhibitory function of the extract was 46% in the root and 49% in the stem at 100 ppm of the extract. The ABTS free radical scavenging function was in the order of HW<70EOH<100EOH

• Journal of Ginseng Research
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• v.47 no.1
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• pp.44-53
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• 2023

Background: The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood. Methods: Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles. Results: The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species. Conclusion: These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.449-455
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• 2022

The Colony of Selaginella involvens (Sw.) Spring in Sanyang-myeon, Mungyeong-si, can be divided into five types, and in the past, it was said that Selaginella involvens (Sw.) Spring formed colonies throughout the colony and grew naturally, but now it remains only in some sections. It was found that the Selaginella involvens (Sw.) Spring colony was damaged by artificial and natural factors, and as an artificial factor, the Selaginella involvens (Sw.) Spring was proven to be effective for medicinal and cremation, and many people were damaging the Selaginella involvens (Sw.) Spring colony without permission. Naturally, vines thrive and Selaginella involvens (Sw.) Spring die, and the reality is that the entire colony of Selaginella involvens (Sw.) Spring is in danger of being damaged if maintenance is not performed. On the other hand, there are sections that reproduce with symbiosis with some herbaceous plants, so it is necessary to plan and implement conservation strategies. In order to preserve the Selaginella involvens (Sw.) Spring colony, CCTV is needed to prevent artificial damage, and on the contrary, install facilities such as fences can easily burn up and create worse scenery, so it can preserve the good environment, restore recoverable areas, and install supplementary buffer zones.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.43-43
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• 2022

우리나라의 많은 자생식물은 약용 및 관상용으로 이용되어 안정적인 공급을 위해서는 적절한 재배 조건에 대한 정보가 필요하다. 본 연구에 사용된 비수리[Lespedeza cuneata (Dum.Cours.) G.Don]와 제비쑥(Artemisia japonica Thunb.)은 각 야관문과 모호라는 이름으로 불리며 약용자원으로서 가치가 높은 식물이나 적정 재배조건에 대한 정보가 부족하다. 따라서 효율적인 재배 조건을 제시하기위해 재배 시 토양, 차광, 추비가 식물의 생육에 미치는 영향을 비교하였다. 실험에 사용된 식물의 종자는 2022년 국립생물자원관에서 분양 받았다. 두 종의 종자를 페트리디시에 파종한 후 15, 20, 25, 30℃(명 또는 암조건)과 25/15℃(16/8h, 명/암) 조건에 파종하여 발아 및 휴면 여부를 확인하였다. 재배 실험은 유리온실에서 원예용상토에 파종한 다음 비수리는 4주, 제비쑥은 6주 동안 생육시킨 균일한 묘를 각 지름 7, 9cm 포트에 이식하여 실험에 사용하였다. 토양실험은 원예상토와 마사토의 비율을 각 1:1, 2:1 및 3:1로 혼합하여 사용하였으며, 차광조건은 무차광, 55, 75%로 설정하였다. 추비 조건은 500 및 1,000ppm 하이포넥스 용액을 재배 6주 후 2주 간격으로 총 3회 엽면시비 하였으며, 원예상토 단용을 모든 실험의 대조구로 사용하였다. 발아실험결과, 비수리는 광조건의 모든 온도에서 발아하였으며, 암조건에서도 유사한 결과를 보였다. 반면 제비쑥은 20℃ 이하의 광조건에서 발아율이 유의적으로 감소하는 경향이었으며, 암조건에서는 모든 온도에서 발아율이 저조하였다. 재배조건 실험 결과, 두 식물 모두 원예상토와 마사토를 3:1로 혼합한 토양이 가장 생육에 적합하였으며, 1,000ppm의 하이포넥스로 추비 처리시 생육이 촉진되었다. 반면 모든 차광조건에서는 식물의 생육이 부진한 모습을 보여, 생육에 부정적인 영향을 주었다.

• Korean journal of applied entomology
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• v.62 no.4
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• pp.355-363
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• 2023

In addition to causing direct feeding damage to a variety of greenhouse crops, Frankliniella occidentalis also inflicts indirect harm by facilitating the transmission of the tomato spotted wilt virus. Historically, the prevention of F. occidentalis infestations has relied heavily on pesticide use. However, this approach has led to significant side effects in agricultural ecosystems, including the development of pest resistance and challenges in effective prevention. In response to these issues, research has been directed towards identifying alternative substances that circumvent the tolerance developed against chemical pesticides. Extracts from sixty-seven medicinal plants were prepared by soaking them in water for 24 hours at room temperature. These extracts were then applied to adult F. occidentalis, with particular attention to moutan extract treatment. This treatment demonstrated a 100% insecticidal effect on the first day. The moutan extract, specifically, was prepared using 50% ethanol, after which the ethanol and water were removed via a rotary evaporator. The resultant product was then lyophilized into a powder and used after being diluted with water. In indoor experiments, a 40% diluted solution was sprayed onto F. occidentalis, exhibiting a 100% insecticidal effect 24 hours post-treatment. Furthermore, a pot test indicated a 78% insecticidal effect on the first day of application. Ongoing research includes the analysis of active substances that demonstrate exceptional insecticidal properties and the conduct of on-site validation tests. The application of the aforementioned extract is anticipated to be effective in the prevention of F. occidentalis infestations.

• The Plant Pathology Journal
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• v.40 no.1
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• pp.73-82
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• 2024

Gardenia (Gardenia jasminoides) is a popular and economically vital plant known for its ornamental and medicinal properties. Despite its widespread cultivation, there has been no documentation of plant viruses on gardenia yet. In the present study, gardenia leaves exhibiting symptoms of plant viral diseases were sampled and sequenced by both metatranscriptome and small RNA sequencing. As a consequence, bean common mosaic virus (BCMV) was identified in gardenia for the first time and named BCMV-gardenia. The full genome sequence of BCMV-gardenia is 10,054 nucleotides (nt) in length (excluding the poly (A) at the 3' termini), encoding a large polyprotein of 3,222 amino acids. Sequence analysis showed that the N-termini of the polyprotein encoded by BCMV-gardenia is less conserved when compared to other BCMV isolates, whereas the C-termini is the most conserved. Maximum likelihood phylogenetic analysis showed that BCMVgardenia was clustered closely with other BCMV isolates identified outside the leguminous plants. Our results indicated that the majority of BCMV-gardenia virus-derived small interfering RNAs (vsiRNAs) were 21 nt and 22 nt, with 21 nt being more abundant. The first nucleotide at the 5' termini of vsiRNAs derived from BCMV-gardenia preferred U and A. The ratio of vsiRNAs derived from sense (51.1%) and antisense (48.9%) strands is approaching, and the distribution of vsiRNAs along the viral genome is generally even, with some hot spots forming in local regions. Our findings could provide new insights into the diversity, evolution, and host expansion of BCMV and contribute to the prevention and treatment of this virus.

• Journal of Life Science
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• v.25 no.10
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• pp.1156-1163
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• 2015

The present study investigated the immunomodulatory properties of four different medicinal plants in a cyclophosphamide-treated Balb/c mouse model. One of the four plants, Ulmus macrocarpa, showed partial resistance against immune suppression induced by cyclophosphamide. The bark of U. macrocarpa, commonly known as the Chinese elm, has been used as a pharmaceutical material in Korean traditional medicine to treat bacterial inflammation and induce wound healing. In this study, water extract of U. macrocarpa, named DEU-7, was used for its immunomodulating functional activity. DEU-7 increased the weight of the spleen and the number of splenocytes but did not significantly affect the liver, kidney, and thymus in vivo. A splenocyte viability assay confirmed that DEU-7 influenced ex vivo splenocyte survival. DEU-7 also increased the levels of cytokines, such as IL-2 and IL-4, and immunoglobulins, such as IgM, IgG, and IgA. These results indicated that DEU-7 is involved in the activation of T and B lymphocytes. In addition, DEU-7 was able to maintain the production of cytokines, such as TNF-α, IL-12, and IFN-γ, in the condition of cyclophosphamide-induced immune suppression, suggesting that DEU-7 activated innate immune cells, even under immune suppression. We concluded that DEU-7 aids immunological homeostasis, thereby preventing immune suppression, and aids both innate and adaptive immune response by maintaining the levels of various cytokines and immunoglobulins. Consequently, it is worth investigating the potential of DEU-7 as a supplemental source for immune-enhancing agents.

• Journal of Bio-Environment Control
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• v.20 no.2
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• pp.93-100
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• 2011

Maintenance of adequate soil water potential during the period of crop growth is necessary to support optimum plant growth and yields. A better understanding of soil water movement within and below the rooting zone can facilitate optimal irrigation scheduling aimed at minimizing the adverse effects of water stress on crop growth and development and the leaching of water below the root zone which can have adverse environmental effects. The objective of this study was to evaluate the feasibility of using a portable irrigation controller with an Watermark sensor for the cultivation of drip-irrigated vegetable crops in a greenhouse. The control capability of the irrigation controller for a soil water potential of -20 kPa was evaluated under summer conditions by cultivating 45-day-old tomato plants grown in three differently textured soils (sandy loam, loam, and loamy sands). Water contents through each soil profile were continuously monitored using three Sentek probes, each consisting of three capacitance sensors at 10, 20, and 30 cm depths. Even though a repeatable cycling of soil water potential occurred for the potential treatment, the lower limit of the Watermark (about 0 kPa) obtained in this study presented a limitation of using the Watermark sensor for optimal irrigation of tomato plants where -20 kPa was used as a point for triggering irrigations. This problem might be related to the slow response time and inadequate soil-sensor interface of the Watermark sensor as compared to a porous and ceramic cup-based tensiometer with a sensitive pressure transducer. In addition, the irrigation time of 50 to 60 min at each of the irrigation operation gave a rapid drop of the potential to zero, resulting in over irrigation of tomatoes. There were differences in water content among the three different soil types under the variable rate irrigation, showing a range of water contents of 16 to 24%, 17 to 28%, and 24 to 32% for loamy sand, sandy loam, and loam soils, respectively. The greatest rate increase in water content was observed in the top of 10 cm depth of sandy loam soil within almost 60 min from the start of irrigation.

• Journal of Biosystems Engineering
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• v.6 no.1
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• pp.15-27
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• 1981

In order to investigate on the field performance of 3.5PS power tiller and to obtain the optimum using conditions of it, this experiment has been carried on the plowing operation, the rotary harrowing operation and the ridging operation that compared 3.5PS power tiller(KC-450) with the existing 5PS(DT-40) and 8PS(DT-85) power tiller. It has been performed at Suck Jung-Dong, Ansung Country, from March to October in 1980. The results of this field test were obtained as follows; 1. The plowing performance of KC-450 tiller was found to be 125min/10a that was lower than that of DT-85 tiller by 26min/10a in the effective plowing speed 0.9m/sec. 2. The field efficiency of KC-450 tiller was the highest among the power tillers as to be 82 persent that was higher than that of DT-85 tiller by 5 persent in the effective plowing speed 1m/sec. 3. The rotary harrowing performances of DT-40 tiller and DT-85 tiller were higher than that of KC-450 tiller in the rotary harrowing operation of the same speed, and the field efficiency of KC-450 tiller and DT-40 tiller were higher than that of DT-85 tiller in the rotary harrowing operation. 4. The ridging Performance of testing power tillers were nearly equality in the ridging operation of the same speed, and the field efficiency of testing tillers were higher KC-450 tiller than DT-40 tiller, DT-40 tiller than DT-85 tiller. 5. The work performance of power tiller increased with increase in the size of field area. Work performance and field efficiency of KC-450 tiller were higher than other tillers below 8a in field size 6. DT -85 tiller was more useful in deep plowing depth. But below 8a in field size KC-450 tiller was more easily operated and its field efficiency was higher 7. KC-450 tiller should be more useful to operate in the small size field or in the small scale farming and for the cultivation of vegetables, gardening plants, medicinal herb and tobacco plants, or for the cultivation in vinyl house.

• Korean Journal of Pharmacognosy
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• v.7 no.3
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• pp.179-190
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• 1976

1. The Korean Acanthopanax genus includes 12 kinds consisting of 9 species and 3 forma. 2. The Korean Oga-pi which is on market sale has been used as bark for the medicinal purpose, and Oga-pi shall use Radicis Cortex. That is why it is basic rule that herbalogy shall use Radicis Cortex. 3. The origin of Oga-pi on sale is Acanthopanax sessiliflorum forma chungbunensis C.S. Yook. 4. $C_{HUNG}\;and\;N_AKAI'S}$ report on A. koreanum told us that there are brown hair on the mid-leaf junction, but in addition to it, our investigation was resulted in the fact that there are thorn along mid-rib sometimes. 5. 2 kinds of new forma are similar to A. sessiliflorum, but are different in the view-point of chemotaxonomy, compared with A. sessiliflorum. In its morphology, we can find some difference between 2 kinds of new forma and A. sessiliflorum. Our effort of examination on documents tell us that the all plants growing in the central part of our country is A. sessiliflorum forma chungbunensis C.S. Yook. The one which has thorn on both side among the plants collected in Mt. Dukyu, is called A. sessiliflorum forma nambunensis C.S. Yook. 6. A. sessiliflorum is growing in the southern part in Korea, and most Chungbu Oga-pi A. sessiliflorum forma chungbunensis in the central part of our country. For the convenience of our study, the key of Korean Acanthopanax plant is classified into, I-IV, as shown on the following items: I. No hair on both side of leaf A. Flower stalk is longer than petiole, and there are thorn under the petiole (5-7 stigma).${\cdots}A.\;sieboldianum$. B. Flower stalk is longer than petiole, or same length. The serration lie down, and the stem has short thorn (stigma is divided into 3 part).${\cdots\;\cdots}A.\;seoulense$ II. There are a lot of thorn or hair on back of leaf. A. A lot of thorn and hair on the vein of leaf back, and a number of small thorn on petiole.${\cdots}A.\;chiisanensis$. B. There are thorn on the vein of leaf back.${\cdots\;\cdots}A.\;sessiliflorum\;forma\;chungbunensis.$ III. There are hairs on both side of leaf. A. There are small hairs on the back of leaf.${\cdots\;\cdots}A.\;sessiliflorum.$ B. There are small hairs on both side of leaf.${\cdots\;\cdots}A.\;sessiliflorum\;f.\;nambunensis.$ C. There are thick hairs on junction of main vein on back of leaf.${\cdots\;\cdots}A.\;koreanum.$ D. There are brown hairs on vein of leaf back, and brown hairs on small petiole.${\cdots\;\cdots}A.\;rufinerve.$ E. There are shrunk hairs in grey-brown on back of leaf, and tense hairs on new branch (one stigma).${\cdots\;\cdots}A.\;divaricatum.$ IV. There are long thorn, just like needles, on the stem and petiole. A. Long needle grows on whole stem tensely, and long needles on petiole.${\cdots\;\cdots}A.\;senticosus.$ B. There are no needles, just like needles aid hairs on petiole, and needles grow on the stem thinly.${\cdots\;\cdots}A.\;asperatus.$ C. There are no needle on small brarch, leaf and inflorescence are larger than A. senticosus. ${\cdots\;\cdots}A.\;senticosus\;forma\;inermis.$.