Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis

  • Hyunjin, Koo (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Yun Sun, Lee (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Van Binh, Nguyen (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Vo Ngoc Linh, Giang (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Hyun Jo, Koo (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Hyun-Seung, Park (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Padmanaban, Mohanan (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Young Hun, Song (Department of Agricultural Biotechnology, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University) ;
  • Byeol, Ryu (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Kyo Bin, Kang (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Sang Hyun, Sung (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University) ;
  • Tae-Jin, Yang (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2021.10.11
  • Accepted : 2022.07.11
  • Published : 2023.01.02
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Abstract

Background: The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood. Methods: Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles. Results: The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species. Conclusion: These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ015903)" Rural Development Administration, Republic of Korea. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2021R1A4A1032888 to Y.H.Song and T.J.Yang).

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