• 한국약용작물학회:학술대회논문집
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• 2009.05a
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• pp.409-410
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• 2009

• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.95-99
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• 2007

The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at $46^{\circ}C$ for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.

• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.100-104
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• 2007

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; $OD_{600}$ = 0.8), co-cultivation period (3 days), and acetosyringone concentration $(100\;{\mu}M)$ had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.506-509
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• 2014

This study was conducted to identify transition characteristics of mercury in several selected medicinal plants and to find the appropriate management for production of safety food. Cultivated soils and medicinal plants were collected at 29 sites for Angelica gigas (Korean angelica root), 68 sites for Platycodon grandiflorum (Balloon flower), 35 sites for codonopsis lanceolata (Deoduck), 36 sites for Dioscorea batatas (Chinese yam), 32 sites for Rehmannia glutinosa (Foxglove), 16 sites for Cnidium officinale makino (cnidium), and 26 sites for Astragalus membranaceus (milk vetch root) during the harvest season of 2013. Mercury in the soils and medicinal roots were analyzed with a Direct Mercury Analyzer. Average content of mercury in soils cultivated medicinal plants was $0.023mg\;kg^{-1}$ (range: from 0.003 to $0.074mg\;kg^{-1}$) and average content of mercury in medicinal plants was $0.003mg\;kg^{-1}$ (range: from 0.001 to $0.011mg\;kg^{-1}$), indicating that mercury in the surveyed soils and medicinal plants were not exceeded the Korean regulation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.95-95
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• 2018

In this study, we investigated the effect of branch extracts from Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}-MSH$) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein level in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, it is thought that VOB may stimulate melanin synthesis through activating tyrosinase activity.

• Bulletin of the Korean Chemical Society
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• v.29 no.10
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• pp.1977-1982
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• 2008

Novel bicyclo[3.1.0]hexanyl purine nucleoside analogues were synthesized as potential antiherpetic agents via a bicyclo[3.1.0]hexanol (${\pm}$)-8, which was prepared using a highly efficient carbenoid cycloaddition reaction. A highly diastereoselective reduction of ketone and a Mitsunobu reaction for the condensation of glycosyl donor (${\pm}$)-12 with 6-chloropurine were employed.

• The Korea Journal of Herbology
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• v.21 no.3
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• pp.35-45
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• 2006

Objectives : The plans can be used for medicinal purposes among Alismatales in korea and examined their effects and distributions. Methods : The examined herbalogical books and research papers which published at home and abroad. Results : 1. There were totaled to 15 genera and 45 species in Alismatales in Korea and among them medicinal plants are 8 genera, 17 species, some 38% in total. 2. Potamogeton genus is main kind enough that it has 14 species among 45 species in the Alismatales , of which medicinal plants are 6 species. 3. The herb is the main medicinal parts of medicinal plants in the Alismatales which is used in 14 species. 4. According to nature and flavor of medicinal plants in the Alismatales , they were classified into cool 8 species; sweet taste 10 in the order. 5. According to meridian propism of medicinal plants in the Alismatales, they were classified into liver meridian 8 species. 6. According to the properties and principal curative action, they were classified into drugs for antifebrile 12 species, drugs for detoxicant 11 , drugs for drain damp 9 in the order. 7. It was noticed that every medicinal plant in the Alismatales is nontoxic. Conclusion : There were totaled to 15 genera and 45 species in Alismatales in Korea and among them medicinal plants are 8 genera, 17 species, some 38% in total.

• The Korea Journal of Herbology
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• v.23 no.1
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• pp.39-44
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• 2008

Objectives : Through surveying the vegetation of woody plants naturally growing in the Chonnam Area. we expect to make good use of the surveyed data for the conservation and utilization of woody plants in the Chonnam Area. And also we hope that we could make a good opportunity to love and conserve the Chonnam Area through this report. Methods : we inspected native and cultivated medicinal woody plants in the Chonnam Area by report and survey, and researched medicinal part through the herbalogy literature. Results : 1. According to the investigation of medicinal woody plant in the Chonnam area, it bears 189 species, 139 genera and 62 families. 2. Rosaceae and Quercus are the biggest share of medicinal woody plants in the Chonnam area. 3. In aspect of medicinal part, roots of 100 species, leaves of 102 species, fruits of 80 species, and barks of 67 species have been using. The leaves part of the most plants are abundantly used. 4. About 30 species of medicinal woody plants are distributed distinctively in Korea. Conclusions : According to the investigation of medicinal woody plant in the Chonnam area, it bears 189 species, 139 genera and 62 families.

• Bulletin of the Korean Chemical Society
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• v.29 no.12
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• pp.2487-2490
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• 2008

• Journal of Information Processing Systems
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• v.13 no.5
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• pp.1103-1125
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• 2017

This paper aims to extract an ObjectProperty-UsageMethod relation, in particular the HerbalMedicinalProperty-UsageMethod relation of the herb-plant object, as a semantic relation between two related sets, a herbal-medicinal-property concept set and a usage-method concept set from several web documents. This HerbalMedicinalProperty-UsageMethod relation benefits people by providing an alternative treatment/solution knowledge to health problems. The research includes three main problems: how to determine EDU (where EDU is an elementary discourse unit or a simple sentence/clause) with a medicinal-property/usage-method concept; how to determine the usage-method boundary; and how to determine the HerbalMedicinalProperty-UsageMethod relation between the two related sets. We propose using N-Word-Co on the verb phrase with the medicinal-property/usage-method concept to solve the first and second problems where the N-Word-Co size is determined by the learning of maximum entropy, support vector machine, and naïve Bayes. We also apply naïve Bayes to solve the third problem of determining the HerbalMedicinalProperty-UsageMethod relation with N-Word-Co elements as features. The research results can provide high precision in the HerbalMedicinalProperty-UsageMethod relation extraction.