• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.303-311
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• 2002

This study evaluated the concentrations of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) in industrial waste incineration workers. The effect of genetic polymorphisms of xenobiotic metabolism enzymes on urinary concentration of PAH metabolites was assessed. And, aromatic DNA adduct levels were also determined in total white blood cells. Fifty employees were recruited from a company handling industrial wastes located in Ansan, Korea: non-exposed group (n=21), exposed group (n=29). Sixteen ambient PAHs were determined by GC/MSD (NIOSH method) from personal breathing zone samples of nine subjects near incinerators. Urinary 1-hydroxypyrene glucuronide (1-OHPG), a major pyrene metabolite, was assayed by synchronous fluorescence spectroscopy after immunoaffinity purification using monoclonal antibody 8E11 (SFS/IAC). Multiplex PCR was used for genotyping for GSTMI/TI and PCR-RFLP for genotyping of CYP1A1 (MspI and Ile/Val). PAH-DNA adducts in peripheral blood WBC were measured by the nuclease P1-enhanced postlabeling assay. Smoking habit, demographic and occupational information were collected by self-administered questionnaire. The range of total ambient PAH levels were 0.00-7.00 mg/㎥ (mean 3.31). Urinary 1-OHPG levels were significantly higher in workers handling industrial wastes than in those with presumed lower exposure to PAHs (p=0.006, by Kruskal-Wallis test). There was a statistically significant dose-response increase in 1-OHPG levels with the number of cigarettes consumed per day (Pearson correlation coefficient=0.686, p<0.001). Urinary 1-OHPG levels in occupationally exposed smoking workers were highest compared with non-occupationally exposed smokers (p=0.053, by Kruskal-Wallis test). Smoking and GSTMI genotype were significant predictors for log-transformed 1-OHPG by multiple regression analysis (overall model R²=0.565, p<0.001), whereas smoking was the only significant predictor for log-transformed aromatic DNA adducts (overall model R²=0.249, p=0.201). Aromatic DNA adducts was also a significantly correlation between log transferred urinary 1-OHPG levels (pearson's correlation coefficient=0.307, p=0.04). However, the partial correlation coefficient adjusting for Age, Sex, and cigarette consumption was not significant (r=0.154, p=0.169). The significant association exists only in individuals with the GSTMI null genotype (pearsons correlation coefficient=0.516, p=0.010; partial correlation coefficient adjusting for age, sex, and cigarette consumption, r=0.363, p=0.038). Our results suggest that the significant increase in urinary 1-OHPG in the exposed workers is due to higher prevalence of smokers among them, and that the association between urinary PAH metabolites and aromatic DNA adducts in workers of industrial waste handling may be modulated by GSTMI genotype. There results remain to be confirmed in future larger studies.

• 핵의학기술
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• 제12권3호
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• pp.147-156
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• 2008

목적 : 방사성옥소 치료팀에서 이루어지고 있는 모든 행위가 나름대로의 원칙과 규정이 있으므로 단일화 된 모델은 적절한 최적화가 아닐 것으로 생각되나 통일성 있는 기준제시에 접근하고자 함이다. 실험재료 및 방법 : 2007년 11월 5일부터 11월 17일까지 29개 병원을 대상으로 핵의학 담당자에게 설문조사하였다. 설문조사의 내용은 환자에 대한 진료부문, 환자관리, 검사 부문, 폐기물관리, 안전관리 등 5개의 카테고리로 정하였으며 질문에 대한 응답내용을 선택하는 방식으로 조사하였다. 결과 : 2007년 10월 기준으로 전체 30개 병원에서 58개의 병실을 운영하고 있었으며 치료계획에 따른 검사 및 일정관리는 약간의 차이가 있었다. 환자에 대한 교육부문은 의사 및 간호사가 주로 담당하였으며 교육에 대한 환자의 만족도는 높은 것으로 평가되었다. 폐기물 관련 자료와 정기적으로 실시되고 있는 정기 감사로 인하여 각 병원별 폐기물 관리 수행은 원만히 이루어지고 있는 것으로 나타났다. 환자 및 작업종사자들에 대한 안전관리는 원자력법에 근거하여 잘 수행되고 있었으며 치료병실에 대한 오염관리에 대해서 대부분 병원에서 철저하게 수행하고 있는 것으로 나타났다. 결론 : 항목별 자료 수집내용을 충분히 검토하여 현실성 있는 설문조사를 통해 구체적인 표준안이 제시되었어야 하나 그러지 못한 부족함이 많다. 그럼에도 불구하고 본 조사에 대해 임상의 활용도 가치를 응답자 중 70.9%로 비교적 높이 평가해 주심에 감사를 표한다. 향후 본 자료를 토대로 각 항목별 세부사항에 대한 조사와 연구가 필요할 것으로 본다.

• 청정기술
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• 제29권2호
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• pp.95-101
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• 2023

폐굴껍질을 원료로 vaterite 형 침강성 탄산칼슘 제조 실험을 하였다. 굴껍질을 800℃의 온도에서 산화칼슘이 주성분인 소성 굴껍질을 제조하였다. 이 굴껍질을 질산이나 염산 용액에 녹여 0.1 M 질산칼슘이나 염화칼슘 수용액을 만들고, 여러 실험 조건에서 0.1 M 탄산나트륨 수용액과 탄산화 반응을 시켰다. 실험 조건은 aspatic acid 첨가량, 반응온도, 교반속도, NH₄OH 첨가량, 반응시간, 용해 산 종류 등이다. XRD, SEM, Size 분석을 하고 vaterite 함유량을 계산하였다. 25℃, 600 rpm, aspatic acid 0.1몰/ 1몰 CaO과 2 cm³의 NH₄OH를 첨가한 최적 조건에서 1시간 반응에서 vaterite 함유량 95.9%의 구형의 침강성 탄산칼슘을 합성하였다. 평균 입경은 12.11 ㎛이었다. 고 vaterite 함유 탄산칼슘은 의료용, 식용, 잉크첨가제 등 고부가가치 탄산칼슘으로 활용된다.

• 한국환경과학회지
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• 제22권11호
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• pp.1457-1464
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• 2013

We investigated the optimal conditions for keratinase production by feather-degrading Pseudomonas geniculata H10 using one variable at a time (OVT) method. The optimal medium composition and cultural condition for keratinase production were determined to be glucose 0.15% (w/v), beef extract 0.08% (w/v), $KH_2PO_4$ 0.12% (w/v), $K_2HPO_4$ 0.02% (w/v), NaCl 0.07% (w/v), $MgSO_4{\cdot}7H_2O$ 0.03%, $MgCl_2{\cdot}6H_2O$ 0.04% along with initial pH 10 at 200 rpm and $25^{\circ}C$, respectively. The production yield of keratinase was 31.6 U/ml in an optimal condition, showing 4.6-fold higher than that in basal medium. The strain H10 also showed plant growth promoting activities. This strain had ammonification activity and produced indoleacetic acid (IAA), siderophore and a variety of hydrolytic enzymes such as protease, lipase and chitinase. Therefore, this study showed that P. geniculata H10 could be not only used to upgrade the nutritional value of feather wastes but also useful in situ biodegradation of feather wastes. Moreover, it is also a potential candidate for the development of biofertilizing agent applicable to crop plant soil.

• 대한영양사협회학술지
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• 제7권1호
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• pp.87-104
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• 2001

This study was conducted as a part of the project developing the standards of national board test for the dietitian's licence. The purpose of this study was to define the job description of dietitians, and to describe the task elements based on the detailed analysis of the dietitians' work. This study team established the research team which was composed of food and nutrition experts, 7 of registered dietitians and 3 of university professors. Draft job description form was made to fully explain the dietitians' task elements, process and sequence by the research team. Final job description form was confirmed after the validity of 576 task elements was reevaluated upon 4 point scale test based on the frequency, importance, and difficulty by 21 field registered dietitian by the study team. It was also confirmed by the university. This form was formated by following DACUM method which analyzed the job description containing duty, task and task elements. It was organized to include every dietitians' work and to describe the every detailed process of work. Duty was defined as the specific and independent work as a dietitian, and was composed of 17 parts, i.e 10 parts in food supply management area and 6 parts in nutritional care service area and self promotion area. Duties were also divided into 99 tasks containing the 576 detailed task elements to describe definite action, steps and decisions. Seventeen parts are following; menu planning, storage and inventory control, food production, meal service, waste management, sanitation, equipment and facility management, human resource management, financial management, nutrition assessment of life cycle, nutritional assessment of several disease, nutritional assessment in specific condition, medical nutrition therapy, nutritional education, public health nutrition and self promotion. If these protocols are properly performed and presented, they can provide the likelihood that dietetics professionals work as a primary contributors in promoting health care and preventing disease nationally.

• Environmental Analysis Health and Toxicology
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• 제28권
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• pp.17.1-17.3
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• 2013

The fact that hazardous metals do not bio-degrade or bio-deteriorate translates to long-lasting environmental effects. In the context of evidently rapid global industrialization, this ought to warrant serious caution, particularly in developing countries. In the Republic of Fiji, a developing country in the South Pacific, several different environmental studies over the past 20 years have shown levels of lead, copper, zinc and iron in sediments of the Suva Harbor to be 6.2, 3.9, 3.3 and 2.1 times more than the accepted background reference levels, respectively. High levels of mercury have also been reported in lagoon shellfish. These data inevitably warrant thorough assessment of the waste practices of industries located upstream from the estuaries, but in addition, an exposure and health impact assessment has never been conducted. Relevant government departments are duty-bound, at least to the general public that reside in and consume seafood from the vicinities of the Suva Harbor, to investigate possible human effects of the elevated hazardous metal concentrations found consistently in 20 years of surface sediment analysis. Furthermore, pollution of the intermediate food web with hazardous metals should be investigated, regardless of whether human effects are eventually confirmed present or not.

• Journal of Plant Biotechnology
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• 제35권1호
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• pp.31-39
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• 2008

We analyzed the current research trends of living modified organisms (LMO) by questionnaires in the interest of making biosaftey laws and policies in Korea. We executed a pre-survey at the Crop Functional Genomics 2004 conference and obtained LMO research information from 423 LMO research organizations, including 32 national research institutes, 314 universities, and 77 industries. We found that the total 59 kinds of hosts including 26 kinds of plants, 15 kinds of animals, and 18 kinds of microbes were used for LMO research and E. coli was the most common host. The risk of the most experimental hosts was below a biosafety level of 1 (73.8%) and 2 (25.9%). LMO development use purpose was implemented in various developmental uses: 51.3% in test and research use, 19% in health and medical use, and 12.9% in agriculture use. The experiment product, waste product, and products of host for LMO development were 327.2, 223.6, and 13.5 in number of plants; 280.6, 52.4, and 8.7 in number of animals; and $8.3\;{\times}\;10^{11}CFU$, $7.7\;{\times}\;10^{11}CFU$, and $6.5\;{\times}\;10^{11}CFU$ in microbes in 2004. The survey results about how to possess the LMO were very unreliable, because only 10.6% of the researchers returned the questionnaires. Consequently, we strongly suggest the scientific organizations as well as scientists should have more interests in biosafety of LMO research and an LMO biosafety management system should be developed for Korea's future biotechnology.

• 한국컴퓨터정보학회논문지
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• 제19권8호
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• pp.19-26
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• 2014

신뢰성 있는 물 수요예측을 실시하기 위해서는 실측자료를 이용하여 다양한 수요구조의 변화를 합리적으로 반영할 수 있는 수요예측모형을 개발 활용하는 것이 필요하다. 본 연구에서는 가정에서사용하고 있는 욕실 내 용수사용량 특성을 파악하기 위하여 전국 140여개 가구를 대상으로 전자식 유량계와 무선송신시스템이 결합된 원격측정시스템을 이용하여 실측자료를 취득하고, 이를 이용하여 각 사용량의 기준이 되는 원단위를 도출하였다. 향후 사용량 예측을 위하여 욕실 내 용수를 욕조용수와 세면용수로 구분하여 시계열 모형을 적용함으로써 물 수요관리 및 정책수립을 위한 정보로서 활용할 수 있도록 하였다.

• 한국치위생학회지
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• 제16권2호
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• pp.313-319
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• 2016

Objectives: The objective of the study was to infection control by analyzing the influence of working environment on infection control activities in the dental hygienists. Methods: The cross-sectional study was based on a survey on a total of 377 dental hygienists working in dental settings. Multiple linear regression analysis was performed to examine the relationship of general characteristics and infection control activities. All statistical analyses were performed using the SPSS for Windows version 20.0, and p<0.05 was considered to be significant. Results: Predictive powers(=Adjusted $R^2$ of the investigated factors such as operation room, sterilization disinfection laundry, dental unit waterline, staff individual, infection control system, personal protective equipment, medical waste, hand hygiene, oral surgical procedures, clinical contact surfaces were adjusted $R^2=0.394$, 0.306, 0.277, 0.244, 0.241, 0.177, 0.165,, 0.154, 0.134, 0.124 respectively. Conclusions: In order to enhance infection control activities, the program development and implementation for the aseptic procedure is very important. The program should include periodic reinforcement of infection control education and regular monitoring of infection control activities.

• 대한방사선기술학회지:방사선기술과학
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• 제8권2호
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• pp.29-45
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• 1985

Computed Tomography Scanner (CT) is highly expensive in operation as well as purchasing. That reason may cause not only to increase the burden of patients but also to waste the capital resources leading to financial difficulties. However the numbers of CT installed throughout the country is increasing, because of efficiency in medical care, patient's concern, competitions among the hospitals within the same area. In the non-Metropolitan area the scanners were expected to be less utilized and less profitable. Nine hospitals equipped with the CT were studied on the utilization of that equipment during the period from November 1984 to February 1985 in non-Metropolitan area and break-even point in one hospital was analyzed for estimating profitabilities. The results were as follows ; 1. Among those nine hospitals, four hospitals had less than 400 beds, which is one of the restrictive minimum standards on the installation of Whole-body Computed Tomography Scanner. 2. The operating time during the normal operation period was longer than those of any other studies, but the accumulated down time was also longer than those of any other studies. The average number of scanning per week for each CT was 45, while the estimated number of for the break-even point was 56.7 cases. 3. When the downtime was excluded in calculating the average operation would be much closer to the cases for the break-even point. Therefore the break-down of the equipment was to be a main cause of the low profitability. 4. The average scanning rate for head area was 33.6%, however three of the nine hospitals showed about 20%. 5. If scanning ratio for the body parts excepting head was increased, the number of scanning for the break-even point would be diminished. 6. The small size hospital especially located near the Metropolitan area showed largest loss in the CT operation. In purchasing the highly expensive equipments in hospitals, demand should be taken into account and planning is recommended.