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Observations on Some of the Mycelial Growth and Pigmentation Characteristics of Cordyceps militaris Isolates

  • Shrestha, Bhushan;Lee, Won-Ho;Han, Sang-Kuk;Sung, Jae-Mo
    • Mycobiology
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    • v.34 no.2
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    • pp.83-91
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    • 2006
  • Characteristic growth patterns of Cordyceps militaris isolates on various media, under varying light conditions and at varying incubation periods were examined. Light was found to be the most critical single factor in determining the density, texture, and pigmentation of the mycelial culture of the fungus. However, under the light condition, the degree of pigmentation and mycelial density were found to be affected by the incubation period and type of medium. Irrespective of the variations in medium type or incubation period, there was no pigmentation of the mycelium under dark condition. Radial growth of the mycelium was faster under dark incubation rather than under light incubation. Abundant mycelial density and darkest pigmentation of C. militaris isolates were produced in nutritionally rich media like SDAY, SMAY and CZYA, suggesting that these media may fulfill all the requirements for vegetative growth of the fungus. Growth characteristics of C. militaris isolates could be easily observed by the simple agar culture method, which would be useful to characterize the phenotypic characteristics of large number of pure cultures of the fungus under given conditions of growth factors such as medium, light and temperature.

A Study on the Layout Type and Space Size in Elementary School Library (초등학교 도서실의 배치유형과 실내공간 규모에 대한 연구)

  • Heo Young-Hwan;Lee Sang-Ho
    • Korean Institute of Interior Design Journal
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    • v.14 no.4 s.51
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    • pp.79-86
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    • 2005
  • According to the new multi-media appeared as a result of rapid growth of the information and science technique, the school library must be changed to the integration of the teach-study media resource from the existing print media. It means that the school library has to play a role of the study center with a different media from the existing teaching material, teaching instrument, facility and opportunity. The Information education must be conducted in the way of an integrated education course and able to do both information transaction and problem settlement. The education facility to solve this problem is the school library. Therefore, the school library becomes important more and more. It means that the school library is the center of entire study and education material for the teacher and student is focused on it. It is urgent to make the standard of the school library In order to cope with the new education and information environments. And especially it is very important to make a plan about the location and scale of the school library to improve the quality of the children's studying activity. The location of the school library is decided under consideration of the relationship with other room of school house and the proper scale of it is between 2.5units and 7.0units of classroom based on the number of class.

Drainage and Treatment Characteristics of Runoff by Media (여재에 따른 도로면 유출수의 배수 및 처리특성)

  • Kim, Seog-gu;Yun, Sang-Ieen;Kim, Young-im;Lee, Yong-jae;Kim, Ree-ho;Kim, Jong-oh
    • Journal of Korean Society on Water Environment
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    • v.20 no.5
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    • pp.500-505
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    • 2004
  • While more attention has been paid in recent years to urban point source pollution control, no considerable measures have been taken to control urban non-point source pollution. Contaminants from non-point sources deposited on urban impermeable surfaces such as road and highway are easily released to the public waters by rainfall, leading to the deterioration in water quality in urban area. The present study investigated a system using filter media to reduce the level of contamination in urban road runoff. Three different media, designated as J-P (polypropylene, blowing type), B-P (polypropylene, fiber type), and P-F (Polyvinyl alcohol, cube and sheet type) were evaluated on the basis of pollutant removal efficiency as well as hydraulic property. Experiments were peformed using a 1/4-scale treatment unit at various pollutant roading and rainfall intensity with real rainwater runoff collected from road surfaces. The results at different rainfall intensities shows that the medium J-P is superior to the other media for water permeability. J-P was not blocked at 10 mm/hr rainfall and its permeability was stable. On the other hand, B-P and P-F resulted in substantial overflow ratio even at I mm/hr rainfall intensity. Comparing treatment efficiencies for suspended solids, all media showed similar removal ranged from 91 % to 95%. However, J-P seems to be better than the other media considering its higher water permeability. J-P and P-F, both hydrophobic, yielded over 76% of heavy metal removal. But, the hydrophilic media B-P showed much smaller removal ranging from 26% to 47%. This indicates that J-P and P-F are beneficial to remove heavy metal due to their hydrophobic property. The treatment efficiencies over PAHs (poly aromatic hydrocarbons) showed the similar removal efficiency ranging from 66% to 97% for all three media.

Crisis Communication on Social Media during COVID-19 Pandemic: An Analysis of Facebook and YouTube (코로나19 상황에서의 소셜미디어를 활용한 위기 커뮤니케이션: 주요국의 페이스북 및 유튜브 활용 비교)

  • Kim, Sohui;Kim, Dongyeon;Ryu, Min Ho
    • Journal of Korea Society of Industrial Information Systems
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    • v.26 no.6
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    • pp.47-60
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    • 2021
  • Since the outbreak of COVID-19 in 2019, the pandemic has been prolonged. This study compares and analyzes the degree of social media usage, the information type of posts (infectious disease information, promote action, psychological communication), and the level of user engagement in conducting crisis communication by country. We conduct text analysis by collecting information on Facebook and YouTube posts from January 2020 to March 2021 of disease control and prevention agencies in Korea, US, UK, and EU. As a result, the use of social media in Korea and US is higher than of the UK and EU, and all four countries are using social media as a means to provide infectious disease information and to promote action. Although social media can be a means to reach the public psychologically, such as sympathy and respect, there are no posts of psychological communication type on social media in countries other than the US. User engagement with posts is highest in the promotion action type. This study can help define the importance and role of social media in establishing an infectious disease crisis communication strategy.

A Relationship with Sports Casualwear Consumer′s Fashion life Style and information source (스포츠 캐주얼웨어 소비자의 패션 라이프 스타일과 정보원과의 관계)

  • 박경연;유태순
    • Journal of the Korean Society of Costume
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    • v.50 no.4
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    • pp.103-115
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    • 2000
  • The purpose of this study is to provide consumers with practical information for reasonable purchasing by analyzing apparel purchasing behavior. information source use, demographic differences in terms of life style patterns, moreover, to support apparel manufacturers in producing goods and making a plan by developing more effective advertisements and efficient marketing strategy, such as media strategy. This study targeted 832 men and women wearing sports casual wear. Information source is used to analyze the data and MANOVA, ANOVA. Scheffe is employed for post-inspection and demographic bases are based on the frequency of each type. The followings are the conclusions of this study : 1. In case of print media, TV, PC factor, fashion advocates and individuality advocates most frequently used information source. In case of purchasing experience factor, individuality advocates skewed higher frequency than conformity type. and then, in case of observation information use factor, individuality advocates were ranked as the highest and conformity type as the lowest. In case of human information use factor, fashion advocates and individuality advocates showed higher frequency than practical type and conformity type. 2 In case of female, Individuality advocates was ranked as the highest. In case of male, practical type and conformity type were rank as the highest. Fashion advocates ranked as the lowest in any case. 3. Individuality advocates were ranked as the highest among people aged 14 to 16, practical type among 17 to 19, conformity type among 20 to 23, conformity type among over 20. 4. Middle school students tended to be the individuality advocates, high school students the practical type, university students the practical type and the conformity type, and company workers conformity type.

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Evaluation of vitrification for cryopreservation of teeth

  • Dissanayake, Surangi C.;Che, Zhong-Min;Choi, Seong-Ho;Lee, Seung-Jong;Kim, Jin
    • Journal of Periodontal and Implant Science
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    • v.40 no.3
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    • pp.111-118
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    • 2010
  • Purpose: The aim of this study was to investigate whether vitrification in the cryopreservation of periodontal ligament (PDL) cells could be useful for tooth banking. Methods: In step 1, primary cultured human PDL cells were cryopreserved in 100% conventional cryopreservation media and 100% vitrification media (ESF40 media) in different temperatures for 2 weeks. In step 2, a series of modified vitrification formulae named T1 (75% vitrification media + 25% F-media), T2 (50% vitrification media + 50% F-media) and T3 (25% vitrification media + 75% F-media) were used to store PDL cells for 2 weeks and 4 weeks in liquid nitrogen. MTT assay was performed to examine the viability of PDL cells. Results: Maximum cell viability was achieved in cells stored in 100% conventional cryopreservation media at $-196^{\circ}C$ (positive control group) in step 1. Compared to the positive control group, viability of the cells stored in 100% vitrification media was very low as 10% in all test conditions. In step 2, as the percentage of vitrification media decreased, the cell viability increased in cells stored for 2 weeks. In 4-week storage of cells in step 2, higher cell viability was observed in the T2 group than the other vitrification formulae while the positive control group had the highest viability. There was no statistically significant difference in the cell viability of 2-week and 4-week stored cells in the T2 group. Conclusions: These observations indicate 100% vitrification media is not successful in PDL cell cryopreservation. Conventional cryopreservation media is currently the most appropriate media type for this purpose while T2 media would be interesting to test for long-term storage of PDL cells.

Analysis of partially embedded beams in two-parameter foundation

  • Akoz, A.Yalcin;Ergun, Hale
    • Structural Engineering and Mechanics
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    • v.42 no.1
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    • pp.1-12
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    • 2012
  • In this study, Pasternak foundation model, which is a two parameter foundation model, is used to analyze the behavior of laterally loaded beams embedded in semi-infinite media. Total potential energy variation of the system is written to formulate the problem that yielded the required field equations and the boundary conditions. Shear force discontinuities are exposed within the boundary conditions by variational method and are validated by photo elastic experiments. Exact solution of the deflection of the beam is obtained. Both foundation parameters are obtained by self calibration for this particular problem and loading type in this study. It is shown that, like the first parameter k, the second foundation parameter G also depends not only on the material type but also on the geometry and the loading type of the system. On the other hand, surface deflection of the semi infinite media under singular loading is obtained and another method is proposed to determine the foundation parameters using the solution of this problem.

On the Identification of Strains and the Cultural Characteristics of Flammulina velutipes in Korea (한국산 팽나무 버섯의 계통류별과 그 배양적 특성에 관한 연구)

  • 윤정구
    • Korean Journal of Microbiology
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    • v.12 no.4
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    • pp.159-179
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    • 1974
  • In order to investigate morphological and cultural characteristics of strains in species, Flammulina velutipes, the author collected isolates of Flammultina velutipes at 49 locations in Korea and cultivated these isolates on the various kinds of solid media. After investigating the cultural characteristics, appeared on the various media, he obtained the following results : 1. The variation of colors in the fruit bodies is connected with the variation of climatic environments(composite effects between mean temperature in January and number of rain days of 1mm and over precipitation). The author, therfore, can find out the trend that brown type is distributed in the midland climatic region and yellow type in the southern climatic regoin. 2. Two types can be classified into several strains respectively : the strain of abundant or insufficient productivity, and strain of selectivity or non-selectivity of media. 3. According to the results of mutual comparison of soluble mycelial proteins by disc electrophoresis using polyacrylamide gels, each type has special common protein fractions(brown type : band located at 26..5mm position from surface of gel, yellow type : band located at 24.5mm position from surface of gel), and each strain has special protein fractions too. Therefore this phenomenon seems to support the results obtained by the above-stated morphological and cultural studies. 4. In the adaptability of strains to the temperature, every strain has the nature of growing in lower temperature(the optimum temperature of 20.deg.C to 25.deg.C) except that YI-1 strain has the optimum temperatue of $25^{\circ}C$-26^{\circ}C. And mycelial growth of every strain is discontinued at $35^{\circ}C.$ 5. In the adaptability of strains to the H-ion concentration, every strain has wide adaptable range of H-ion concentration, and has optimum range of pH 5.5 to 6.6 in mycelial growth excepting YA01, BI-2 and YI-1. 6. In the utility of carbon sources, the mycelial growth of every strain is very poor on the media containing xylose(average diameter of mycelial growth : 18mm), and most strains utilize favorably sucrose(39mm), maltose(37mm) and dextrose(35mm) in mycelial growth. In the utility of nitrogen sources, every strain utilizes favorably organic nitrogens(36 mm)more than inorganic nitrogens (25 mm), and utilizes fully peptone nad asparagine in organic nitrogens. Especially BA-1, BIK-2 and YA-1 strains grow vigorously on each media containing various carbon and nitrogen sources. 7. The characteristic tests of the number of days required for mycelial growth, the number of days requried for sprout of young bodies, the length of stipe and the number of fruit bodies formation seem to be useful methods in the early selection of the strain of the abundant productivity.

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Analysis to Select Filter Media and The Treatment Effect of Non-point Pollution Source in Road Runoff

  • Lee, Tae Goo;Han, Young Hae
    • KIEAE Journal
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    • v.14 no.3
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    • pp.55-63
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    • 2014
  • This study selected and analyzed filter media that can be applied in non-point pollution reduction devices aimed at processing the source of pollution on site for road runoff that increases rapidly in rainfall-runoff in order to improve the water quality of urban areas. First, the factors that affect the quality of runoff caused by sources of non-point pollution include physical and social factors such as the usage of land around the area of water collection, type of pavement and movement of cars and people, as well as rainfall characteristics such as frequency, intensity, amount and duration of rainfall. Second, the purification tests of the filter media were processed for pH, BOD, COD and T-P, and the filter media showed to have initial purification effect at that items. However, the filter media showed to be very effective for the processing of SS, T-N, Zn and Cd from the beginning to the end. Third, for filter media, zeolite and vermiculite showed to be effective for processing SS, T-N, Zn and CD constantly, and composite filter media including zeolite showed to have strong processing effects. The authors conclude that this study can be applied to technical areas and policies aimed at reducing non-point pollution in urban areas and can also contribute to allowing eco-friendly management of rainfall as well as improvement of water quality.

Stabile Fermentation of Citric Acid Using Immobilized Saccharomycopsis lipolytica

  • Kim, Eun-Ki;Ronnie S. Roberts
    • Journal of Microbiology and Biotechnology
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    • v.1 no.2
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    • pp.130-135
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    • 1991
  • The effects of media composition on citric acid fermentation using surface immobilized Saccharomycopsis lipolytica were studied. The use of the standard medium for these organisms resulted in rapid decrease of citric acid production and a transformation of immobilized cell morphologies from a yeast-type to a mycelium-type. When the standard medium was enriched with vitamins, trace minerals, a growth factor and ammonium to form a Vigorous Stationary Phase (VSP) fermentation type medium, relatively stable citric acid production (10 mg/lㆍh) was obtained. Using the VSP type medium, the surface immobilized cells also retained their yeast-type form.

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